Sexual conflict predicts morphology and behavior in two species of penduline tits
© van Dijk et al; licensee BioMed Central Ltd. 2010
Received: 9 December 2009
Accepted: 23 April 2010
Published: 23 April 2010
The evolutionary interests of males and females rarely coincide (sexual conflict), and these conflicting interests influence morphology, behavior and speciation in various organisms. We examined consequences of variation in sexual conflict in two closely-related passerine birds with contrasting breeding systems: the Eurasian penduline tit Remiz pendulinus (EPT) exhibiting a highly polygamous breeding system with sexually antagonistic interests over parental care, and the socially monogamous Cape penduline tit Anthoscopus minutus (CPT). We derived four a priori predictions from sexual conflict theory and tested these using data collected in Central Europe (EPT) and South Africa (CPT). Firstly, we predicted that EPTs exhibit more sexually dimorphic plumage than CPTs due to more intense sexual selection. Secondly, we expected brighter EPT males to provide less care than duller males. Thirdly, since song is a sexually selected trait in many birds, male EPTs were expected to exhibit more complex songs than CPT males. Finally, intense sexual conflict in EPT was expected to lead to low nest attendance as an indication of sexually antagonistic interests, whereas we expected more cooperation between parents in CPT consistent with their socially monogamous breeding system.
Consistent with our predictions EPTs exhibited greater sexual dimorphism in plumage and more complex song than CPTs, and brighter EPT males provided less care than duller ones. EPT parents attended the nest less frequently and less simultaneously than CPT parents.
These results are consistent with sexual conflict theory: species in which sexual conflict is more manifested (EPT) exhibited a stronger sexual dimorphism and more elaborated sexually selected traits than species with less intense sexual conflict (CPT). Our results are also consistent with the notion that EPTs attempt to force their partner to work harder as expected under sexual conflict: each member of the breeding pair attempts to shift the costs of care to the other parent. More brightly colored males benefit more from desertion than dull ones, because they are more likely to remate with a new female. Taken together, the comparison between two closely related species with contrasting breeding systems suggest that sexual conflict over care has influenced the evolution of behavior and morphology in penduline tits.
Reproduction has long been viewed as a cooperative exercise between male and female partners. Yet, the evolutionary interests of males and females are often different (sexual conflict, ). Only in the rare case of semelparity, or when there is full and lifelong monogamy of the pair members will the interests, e.g. in mating rate or amount of parental care, be equal for both sexes [2, 3]. Only recently, however, have researchers started to explore the implications of sexual conflict on speciation, breeding systems, and evolution of various life-history traits [4–7]. Sexual conflict is a potent evolutionary force that may mold morphology and behavior [8, 9], and promote speciation . For instance, behavioral traits of dung fly, Sepsis cynipsea, in populations undergoing intense sexual conflict diverged to a greater extent than flies under relaxed conflict, resulting in different levels of reproductive isolation . Extra-pair copulations in monogamous passerines may also result from sexual conflict. The negative selection for direct benefits from extra-pair copulations for females appears to be greater than the positive selection for indirect benefits, which supports the notion that extra-pair copulations reflect pre-zygotic sexual conflict .
Conflicts between parents over care (post-zygotic sexual conflict, ) emerge via a trade-off between parental effort and alternative mating opportunities for each parent. As a result, each parent is expected to avoid the costs of care and shift those costs to its partner [5, 14]. This may happen through a continuous adjustment of parental effort in response to the mate's current effort (best response rule, ), or through a discrete decision to either care for the offspring or to desert the partner and offspring [16, 17]. Conflict over care typically occurs when there is an opportunity to reduce parental contribution. For example, a parent may desert the brood when one parent is sufficient to successfully raise the offspring [17, 18]. This may occur when resources are plentiful  or when offspring require little care, as is often the case with precocial young [20, 21]. By deserting, the parent may benefit from finding a new mate and breeding again, thereby enhancing its reproductive success ([3, 22, 23], but see: ). With increasing levels of polygamy, variance in reproductive success increases and thus polygamous breeding systems are usually associated with intense sexual selection [24–28]. Subsequently, sexual selection is expected to act stronger in species experiencing greater disparity in care provisioning.
Here we test a priori predictions of sexual conflict theory about the impact of sexual conflict on morphology and behavior by comparing two closely related species of penduline tits [29, 30]: the sequentially polygynandrous Eurasian penduline tit Remiz pendulinus (henceforth EPT) and the socially monogamous Cape penduline tit Anthoscopus minutus (henceforth CPT).
Firstly, given that EPT is frequently polygamous and thus likely experiences a larger variance in reproductive success, we predicted more intense sexual selection in EPT than in the socially monogamous CPT. This is expected to result in a stronger sexual plumage dimorphism and more complex song in EPT than in CPT. Both song and plumage are often sexually selected traits in birds, including penduline tits ([39–41], Pogány, Á. et al. unpublished data).
Secondly, we tested the prediction that male EPTs with a wider eye-stripe, which signals male attractiveness [42, 43], desert the nest more often than males with a narrower eye-stripe, thus imposing the costs of parental care on their mate. We expected that males with wider eye-stripes desert more frequently than males with narrower ones, since males with wider eye-stripes more often and more quickly procure new mates after desertion . As such, males with wider eye-stripes should derive greater benefits from desertion to offset the potential cost of biparental desertion. Females mated to males with wider eye-stripes, however, face the costs of care and/or reduced reproductive success (; Figure 1).
Finally, nests of both species are sophisticated structures (see below) and built by both sexes. Following predictions from sexual conflict theory (e.g. ), however, we expected that in EPT, in which nest desertion is common, parents will attempt shifting the costs of care to their mate. Nest desertion by both males and females takes place at around the third day of the egg laying phase [44–46] and the process of desertion appears to be rapid in our population during which either the male or the female may desert first [36, 45]. This is consistent with the prediction from sexual conflict theory that parents attempt to force their mate to work harder . Given the intense conflict in EPT , a parent may abstain from building a nest expecting its mate to make up the shortfall. Specifically, we predicted EPT pairs to attend the nest less frequently and less synchronously during the egg-laying phase than in CPT, in which parents are expected to cooperate over nest attendance. In the cooperating CPT we predicted synchronous nest attendance and coordinated nest building by both parents.
Eyestripe-size of male and female penduline tits.
1.29 ± 0.23 (N = 155)
0.93 ± 0.20 (N = 34)
t = 8.419
0.13 ± 0.02 (N = 9)
0.14 ± 0.03 (N = 8)
Z = 0.627
From the song recordings of EPT (127.5 ± 48.4 min (mean ± SD), 16 males) 2229 syllables were analyzed. Adding all recordings from all males together, the total number of different syllables sung in the population ('repertoire size') in EPT did not increase after 46% of the total recording time. Additionally, after the first 52% of recorded syllables (i.e. the first hour of recording from all 16 males) we obtained 14 out of the 16 different syllables we recorded in total (i.e. 88%). All different syllables sung by an individual male were obtained after 71% ± 24% of the total number of syllables recorded per individual. We may have underestimated the repertoire size for individual males, although this would only make our results more conservative (see below). The song recorded from CPT (220.9 min ± 94.3 min, 9 males) contained a total 1918 syllables. We did not find variation in the number of different syllables sung by CPT, so we are confident that we obtained the full repertoire size for CPT.
Comparing the expected versus observed times at the nest by both parents, we found a significant effect of species (Figure 5; F = 20.366, P < 0.001, N = 28, η2 = 0.439, 1-β = 0.99): EPTs spent significantly less time together at the nest than expected by chance (Figure 5; 0.20% versus 3.23% of time, respectively;Z = ± 4.015, P < 0.001, N = 21 pairs, d = 2.898, 1-β > 0.99), whereas in CPT the expected versus observed times were not different (Figure 5; 5.56% versus 5.05% of time, respectively; Z = ± 0.845, P = 0.398, N = 7 pairs, d = 0.329, 1-β = 0.09, Nrequired = 146, Nd,1-β; = 4). Finally, EPTs spent significantly less time at the nest (36.0 ± 9.9%, N = 21 pairs) than CPTs (49.4 ± 15.7%, N = 7 pairs; F = 7.075, P = 0.013, η2 = 0.214, 1-β = 0.726).
Penduline tits (Remizinae) are emerging as one of the model systems in investigations of parental conflict [4, 23, 31, 48]. Here we found support for several a priori predictions of sexual conflict theory by comparing the behavior and plumage dimorphism of two closely related species. Firstly, we found substantial sexual plumage dimorphism in EPT, but not in CPT. Secondly, we found that EPT males had a more complex song (i.e. a larger song repertoire) than CPT males. These results together suggest that sexual conflict influences the evolution of plumage dimorphism and complexity of song through intensified sexual selection. We realize that the power to detect a significant difference between the sexes of CPT in size of the eye-stripe is low. However, the sample size required to detect a sexual dimorphism in the size of the eye-stripe in CPT, given the effect size and power (Nrequired), is unrealistically large (13581), yet with our sample we would have been able to detect a sex difference in eye-stripe size in CPT if it had been of a similar intensity as in EPT (Nd,1-β; Table 1). Therefore eye-stripes are sexually monomorphic in CPT. We note that EPT males and females can easily be distinguished in the field, but not CPTs (see various field guides), and we thus suspect our results would be robust even with larger samples sizes.
Thirdly, we found that EPT males with wider eye-striped were more likely to desert their mate than males with narrower eye-stripes. However, females mated to males with wider eye-stripes did not provide care more often than those females that were mated to males with narrower eye-stripes. This somehow contradicts the prediction of the differential allocation hypothesis, which predicts that females mated with more attractive males are willing to invest more in the offspring than females mated to less attractive males [49, 50]. One interpretation is retaliation by the females to avoid being exploited by the males: if an attractive male deserts, a female may still desert, despite the loss of the eggs (see also [3, 32]). Nevertheless, although females may obtain direct and/or indirect benefits from mating with attractive males, they also pay the full costs of caring or, in case they desert too, their efforts invested in nest building and egg-laying appear to be in vain. This points to the dilemma of EPT females: by choosing an attractive mate she may actually lose .
Sexual conflict may be associated to a process of manipulation by one parent and resistance by the other. This potentially affects the evolution of various traits [2, 8], and may also explain the difference in sexual dimorphism and song complexity between the two species of penduline tit. Evidence for this arms race between male and female partners derives from pre-copulatory sexual conflict where males are harmful to females during copulation [2, 9, 51, 52]. Males may, for instance, cause genital damage to the female , or force the female to mate at a suboptimal rate [4, 6, 9, 53, 54]. Males may also try to exploit the female's perception system during mate choice and parental investment, for instance through exaggerated sexually selected traits in males, such as ornaments or song. Females are expected to counter-adapt through more selective mate choice [4, 6, 8] leading to female resistance to mating and the evolution of exaggerated male display to overcome this resistance ('sexually antagonistic coevolution', [8, 55]; see also [56, 57]). In that light, the evolutionary driving force of preference is resistance to male-imposed costs, rather than gaining benefits from mating with preferred males, as described under classic sexual selection [6, 8, 55]. Mediated by such a dynamic process male EPTs may try to manipulate their partner via elaborate plumage and song. This, in conjunction with resistance by the female, may have led to the exaggeration of those traits in EPT, but not in CPT.
An alternative explanation for the elaboration of traits in EPT is the higher population density than in CPT and thus more intense selection driven by male-male competition [58, 59]. However, this argument has been challenged [60, 61] and breeding density itself is not a selective process, but rather an environmental trait that amplifies or de-amplifies sexual conflict. For instance, more potential mates may be available when breeding densities are high and a parent may then benefit more from desertion rather than caring for the offspring. We believe sexual conflict is a more parsimonious explanation than the alternatives, because our previous work showed strong evidence of intense sexual conflict in EPT  and the intensity of sexual conflict is unrelated to breeding density in EPT .
Finally, we found that EPT parents not only spend less time overall on nest attendance than CPT, but they also were at the nest less synchronously. The latter was also true when we compared the estimated absolute time the parents spent jointly at the nest, confirming that the difference in day length between our two study sites does not alter our results. These results suggest that EPT parents appear to avoid each other at the nest - consistent with our argument above that one parent may force the partner to work harder. An alternative explanation for the observed pattern in EPT is role division so that EPT partners may take over the job of nest building from each other rather than actively avoiding each other at the nest. This would also result in less time spent together at the nest, albeit that this should be interpreted as a more cooperative behavior as opposed to avoidance due to conflict. The fact that the total time spent on nest attendance (total frequency of individual and joint attendance) is lower in EPT than in CPT, however, corroborates the idea that they actively try to avoid each other, rather than taking over each other's work as a cooperative effort (see ).
An alternative hypothesis, mate guarding [62–64], predicts the opposite pattern to what we found: due to frequent mate change and dense breeding population in EPT, one would predict more intense mate guarding in EPT than in CPT and thus more synchronous appearance at the nest.
We acknowledge alternative selective processes to sexual conflict that may influence the evolution of morphology and behavior by acting themselves or acting with sexual conflict. To establish the generality of these results and to test alternative hypotheses, we need phylogenetic comparative studies using the appropriate framework. We are currently working on the first comprehensive phylogenetic hypotheses for Remizinae (van Dijk et al, in preparation), which will serve as backbone for future analyses [65–67].
We tested a priori predictions from sexual conflict theory using two closely-related species of penduline tits that exhibit different breeding systems. Differences in behavior and morphology between the two species were consistent with the predictions of sexual conflict theory. Although various studies have found support for these predictions in relation to pre-zygotic sexual conflict (sexual conflict over mating), whether the same is true for sexual conflict exhibited after fertilization was hitherto unclear. Detailed data from the field, collected from multiple, closely-related species exhibiting a variety of breeding systems in various habitats will further advance this field. The diverse breeding systems of penduline tits are an excellent model system to understand how sexual conflict and cooperation may have shaped the evolution of morphology, behavior, neuro-endocrinology and genome of organisms.
Study sites and data collection
We studied EPTs between April and August in five consecutive breeding seasons (2003-2007) in a reed marsh at a 1321ha fishpond system, Fehértó, in southern Hungary (46°19'N 20°6'E), where approximately 60-90 males and 45-50 females bred each year. We studied eight and six breeding pairs of CPT in September 2006 and 2007, respectively, in coastal scrubland at the 572ha Koeberg Nature Reserve near Cape Town, South Africa (33°40'S 18°26'E). The low number of monitored nests in CPT compared to EPT is due to the lower population density in CPT, as large territories are used by family groups . Both species build similar, domed nests, initiated by the male. In EPT males are unpaired, whereas most CPT males are paired at the onset of building. The nest is finished and maintained jointly by both male and female after pair formation in both species. The egg-laying phase is initiated at a similar stage of nest building, i.e. when the parents start building the entrance tube to the nest.
We searched both study areas for nest-building penduline tits, and visited each nest about every other day to determine which parent attended the nest . At each EPT nest we recorded the date of pair formation. We considered a male to be mated as soon as the pair was seen copulating near the nest or when male and female were seen to build the nest together. For time in season we used a date format as the number of days since 1 April in each year. We trapped and banded birds with one numbered metal band from the Hungarian Ornithological Institute (EPT) or the South African Bird Ringing Scheme (CPT), and a unique combination of three color bands (A.C. Hughes, Middlesex, UK). Three digital photographs were taken of each side of the bird's head using an Olympus FE-100 and a Fujifilm FinePix A203 digital camera. In all photographs we kept a ruler in the background as a reference to measure the size of the eye-stripes. The birds were hand held touching the ground and the camera was positioned at an approximately fixed distance (about 20 cm) from the bird to standardize aberrations. The area of the eye-stripe (to the nearest 0.01 cm2), signaling attractiveness in EPT [42, 43], was quantified from the digital photographs using Adobe Photoshop 7.0. We took the average of the three measurements for the size of the eye-stripe.
The song of 16 male EPTs was recorded in 2006 for 127.5 ± 48.4 min (mean ± SD) at a randomly selected time of day between 06:28 and 17:50 (CET), using a Marantz PMD 660 portable digital recorder with a Sennheiser ME66 directional microphone. It is worth noting here that Eurasian penduline tits, as well as other species of penduline tits in the genus Remiz, sing little compared to other species of song birds and do not engage in the dawn or dusk chorus (van Dijk, RE, Bot, S, and Pogány, Á, pers. obs.). Using the same equipment as for EPT, we recorded the song of 9 CPT males (recording time 220.9 min ± 94.3 min). All recordings for CPT were made during the morning (06:20 - 11:30 UTC). For both species, in the analyses we only included song recordings from mated males. Sonograms of the recordings were created and analyzed using Audacity v. 1.2.6 and Avisoft-SASLab Light v. 3.74.
To investigate nest attendance during nest building, which continues through the laying period, we filmed nests in 2006 and 2007 in both EPT and CPT using a time-lapse video camera (Sony digital handycam, DCR-HC44E) storing one frame every five seconds. In CPT we knew precisely the date when the first egg was laid, and nest attendance of parents was recorded during the second and third days of egg-laying (547 min ± 82 min per day, N = 7 pairs). In EPT egg-laying dates were often not known, therefore we recorded nest attendance from after pair formation and during egg-laying for EPT (329 min ± 184 min per day, N = 21 pairs), i.e. a more extended period than for CPT. The period before egg-laying involves more nest building than maintenance. We anticipate that this would not influence our results, since the parents are expected to spend more time at the nest during nest building than during nest maintenance, which would result in more nest attendance in EPT than in CPT. The pattern we predicted and found is opposite to this (see Results). Recordings were analyzed frame by frame using MATLAB v. 6.5 (256240 and 96632 frames in total for EPT and CPT, respectively), coding nest attendance (i.e. presence of bird on or inside the nest) as: (i) male-only, (ii) female-only, (iii) joint nest attendance by male and female, or (iv) both parents absent. When EPT parents are inside the nest, the head is still visible. CPT parents often close the entrance spout when they have entered the nest and when they leave it again. This was visible on our video recordings. We were thus able to accurately score the time the parents spent on as well as inside the nest. Behavioral observations carried out for a previous study showed that EPT parents are rarely near the nest together (for instance, one parent building at the nest with the partner perching nearby), so this will not have confounded our results (van Dijk, RE, Szentirmai, I, Székely, T unpublished data). CPT parents, however, are often together near the nest, as is shown by our results.
To distinguish male and female parents from intruders we used individual differences in plumage [43, 68], behavior (e.g. females are more often and for longer periods inside the nest than males; intruders are often on the outside of the nest and build very little), and color bands. Ambivalent records, i.e. when the identity of an individual was ambiguous, were excluded (7.1% and 4.8% of total records of EPT and CPT, respectively). These excluded frames consisted of those frames where an individual was present plus all following frames with none of the parents present until a frame where a new bird appeared that could be identified. The aim of latter was to avoid a bias towards estimated absence. As nest desertion takes place during egg-laying in EPT, we only included pre-desertion records.
We used binary logistic regression models with backward elimination to predict parental care strategy (male or female as response variable; care/desert) at the first clutch of EPT in response to the size of the male eye-stripes. The initial model included year as a categorical covariate and mating date as a continuous covariate. Neither covariate contributed significantly to the model (P > 0.255), so both were removed from the final model. The final model provided an adequate fit to both male strategy (Hosmer-Lemeshow goodness-of-fit; χ2 = 10.289, df = 8, P = 0.245), and female strategy (χ2 = 6.400, df = 8, P = 0.603).
All CPTs videotaped were color banded. The analyses for nest attendance by CPTs included one male that was recorded at two nests in consecutive years with a different female, so we included these as two data. Out of the 21 EPT nests filmed, one male and 18 females were not color banded. Adult returning rates between years are low (5% for males, 2% for females; ), therefore it is unlikely that we observed the same unbanded individuals in different years. Additionally, of eight unbanded females in 2006 and the ten in 2007, three and six bred simultaneously, respectively, and we can thus be certain that these are different individuals. For the remaining nine females we cannot exclude the possibility of pseudoreplication, although we suspect it is unlikely given (i) the size of our breeding population and (ii) that the composition of pairs was nearly always different (out of 194 pairs that produced a clutch, only six pairs remained together and produced a second clutch between 2002 and 2007). Pseudoreplication in the plumage analyses was avoided by randomly choosing one measurement per individual.
To examine the degree of synchrony in nest attendance by male and female, i.e. male and female being together at the nest simultaneously, we first calculated the time that the male and female can be expected to spend together at the nest by chance, by multiplying the total percentage nest attendance by the male, i.e. male-only attendance plus attendance by male and female together, with the total percentage nest attendance by the female. We then compared the difference between observed and expected patterns of nest attendance by both species using a General Linear Model (GLM). A GLM was also used to compare the total proportion of time the parents spent at the nest, i.e. the sum of male-only, female-only and joint nest attendance, between the two species. Both GLMs included year as a factor and the first day of filming as a covariate, although neither contributed significantly to either of the models (P > 0.138) so they were excluded from the final models. Day lengths are different between Hungary (15 h 46 min ± 0 h 11 min, Budapest) and South Africa (11 h 41 min ± 0 h 12 min, Cape Town) and to test whether this might confound our results we estimated the absolute time the parents attended the nest per day as the percentage of time spent at the nest × day length (day lengths for both study sites collected from http://www.timeanddate.com). We then compared whether the absolute time spent at the nest by both parents is different between EPT and CPT.
We provide effect sizes  and power analyses, and applied the asymptotic relative efficiency when estimating power of Mann-Whitney U-tests . If the power of the statistics was relatively low for CPT (i.e. 1-β ≤0.5), we provide the sample size that would be required to find a statistical significant difference between the two groups given Cohen's effect size d of the underlying data of CPT and power 1-β = 0.8 (Nrequired), and the required sample size given the effect size d in EPT and power 1-β = 0.8 (Nd,1-β). All statistical analyses were performed using SPSS 14.0.0 (SPSS Inc., USA), except power analyses, which were carried out in R (R Development Core Team 2005). We provide mean ± SD, and two-tailed probabilities.
We are grateful to all people who helped with the fieldwork in Hungary over the years. We thank the Kiskunság National Park, Szegedfish Ltd. (Hungary), and ESKOM (South Africa) for permission to carry out fieldwork at the field sites. We also thank Johan van der Dennen for writing the MATLAB script to analyze the time-lapse video recordings, and Sander Bot for analyzing EPT song. The research leading to these results has received funding from the European Community's Sixth Framework Programme [FP6/2002-2006] under contract n. 28696. The work received further financial support from a University of Bath studentship and from the Schure-Beijerinck-Poppingfonds of the Royal Netherlands Academy of Sciences [SBP/JK/2007-53] to REvD, from the Hungarian-South African Intergovernmental TÉT [OMFB-00564/2006] to TS, AP, and PL, and from the Hungarian National Science Foundation OTKA [T043390], a Royal Society Joint Project grant , and the Leverhulme Trust [RF/2/RFG/2005/0279] to TS.
- Parker GA: Sexual selection and sexual conflict. Sexual selection and reproductive competition in insects. Edited by: Blum MS, Blum NA. 1979, New York: Academic Press, 123-166.Google Scholar
- Lessells CM: The evolutionary outcome of sexual conflict. Phil Trans R Soc Lond B. 2006, 361: 301-317. 10.1098/rstb.2005.1795.View ArticleGoogle Scholar
- van Dijk RE, Székely T: Post-fertilization reproductive strategies. Encyclopedia of Life Sciences (ELS). 2008, Chichester: John Wiley & Sons, LtdGoogle Scholar
- Arnqvist G, Rowe L: Sexual Conflict. 2005, Princeton, New Jersey: Princeton University PressView ArticleGoogle Scholar
- Houston AI, Székely T, McNamara JM: Conflict between parents over care. Trends Ecol Evol. 2005, 20: 33-38. 10.1016/j.tree.2004.10.008.View ArticlePubMedGoogle Scholar
- Gavrilets S, Arnqvist G, Friberg U: The evolution of female mate choice by sexual conflict. Proc Roy Soc Lond B. 2001, 268: 531-539. 10.1098/rspb.2000.1382.View ArticleGoogle Scholar
- Hosken DJ, Snook RR: How important is sexual conflict?. Am Nat. 2005, 165: S1-S4. 10.1086/429355.View ArticleGoogle Scholar
- Chapman T, Arnqvist G, Bangham J, Rowe L: Sexual conflict. Trends EcolEvol. 2003, 18: 41-47. 10.1016/S0169-5347(02)00004-6.View ArticleGoogle Scholar
- Arnqvist G, Rowe L: Antagonistic coevolution between the sexes in a group of insects. Nature. 2002, 415: 787-789.View ArticlePubMedGoogle Scholar
- Arnqvist G, Edvardsson M, Friberg U, Nilsson T: Sexual conflict promotes speciation in insects. Proc Natl Acad Sci USA. 2000, 97: 10460-10464. 10.1073/pnas.97.19.10460.PubMed CentralView ArticlePubMedGoogle Scholar
- Martin OY, Hosken DJ: The evolution of reproductive isolation through sexual conflict. Nature. 2003, 423: 979-982. 10.1038/nature01752.View ArticlePubMedGoogle Scholar
- Arnqvist G, Kirkpatrick M: The evolution of infidelity in socially monogamous passerines: the strength of direct and indirect selection on extrapair copulation behavior in females. Am Nat. 2005, 165: S26-S37. 10.1086/429350.View ArticlePubMedGoogle Scholar
- Royle NJ, Hartley IR, Parker GA: Sexual conflict reduces offspring fitness in zebra finches. Nature. 2002, 416: 733-736. 10.1038/416733a.View ArticlePubMedGoogle Scholar
- Lessells CM: Sexual conflict in animals. Levels of selection in evolution. Edited by: Keller L. 1999, Princeton, NJ: Princeton University Press, 75-99.Google Scholar
- Houston AI, Davies NB: The evolution of cooperation and life history in the dunnock Prunella modularis. Behavioural Ecology: Ecological consequences of adaptive behaviour. Edited by: Sibley RM, Smith RH. 1985, Oxford: Blackwell Science, 471-487.Google Scholar
- Maynard Smith J: Parental investment: a prospective analysis. Anim Behav. 1977, 25: 1-9. 10.1016/0003-3472(77)90062-8.View ArticleGoogle Scholar
- Székely T, Webb JN, Houston AI, McNamara JM: An evolutionary approach to offspring desertion in birds. Current Ornithology. Edited by: Nolan VJ, Ketterson ED. 1996, Plenum Press, 13: 271-330.View ArticleGoogle Scholar
- Bart J, Tornes A: Importance of monogamous male birds in determining reproductive success: evidence for house wrens and a review of male-removal studies. Behav Ecol Sociobiol. 1989, 24: 109-116. 10.1007/BF00299642.View ArticleGoogle Scholar
- Beissinger SR, Snyder NFR: Mate desertion in the snail kite. Anim Behav. 1987, 35: 477-487. 10.1016/S0003-3472(87)80273-7.View ArticleGoogle Scholar
- Olson VA, Liker A, Freckleton RP, Székely T: Parental conflict in birds: comparative analyses of offspring development, ecology and mating opportunities. Proc Roy Soc Lond B. 2007Google Scholar
- Wisenden BD: Factors affecting mate desertion by males in free-ranging convict cichlids (Cichlasoma nigrofasciatum). Behav Ecol. 1994, 5: 439-447. 10.1093/beheco/5.4.439.View ArticleGoogle Scholar
- Pilastro A, Biddau L, Marin G, Mingozzi T: Female brood desertion increases with number of available mates in the Rock Sparro. J Avian Biol. 2001, 32: 68-72. 10.1034/j.1600-048X.2001.320109.x.View ArticleGoogle Scholar
- Szentirmai I, Székely T, Komdeur J: Sexual conflict over care: antagonistic effects of clutch desertion on reproductive success of male and female penduline tits. J Evol Biol. 2007, 20: 1739-1744. 10.1111/j.1420-9101.2007.01392.x.View ArticlePubMedGoogle Scholar
- Wiklund C, Forsberg J: Sexual size dimorphism in relation to female polygamy and protandry in butterflies: a comparative study of Swedish Pieridae and Satyridae. Oikos. 1991, 60: 373-381. 10.2307/3545080.View ArticleGoogle Scholar
- Gonzalez-Voyer A, Fitzpatrick JL, Kolm N: Sexual selection determines parental care patterns in cichlid fishes. Evolution. 2008, 62: 2015-2026. 10.1111/j.1558-5646.2008.00426.x.View ArticlePubMedGoogle Scholar
- Pérez-Barbería FJ, Gordon IJ, Pagel M: The origins of sexual dimorphism in body size in ungulates. Evolution. 2002, 56: 1276-1285.View ArticlePubMedGoogle Scholar
- Björklund M: A phylogenetic interpretation of sexual dimorphism in body size and ornament in relation to mating system in birds. J Evol Biol. 1990, 3: 171-183. 10.1046/j.1420-9101.1990.3030171.x.View ArticleGoogle Scholar
- Bedhomme S, Bernasconi G, Koene JM, Lankinen Å, Arathi HS, Michiels NK, Anthes N: How does breeding system variation modulate sexual antagonism?. Biol Letters. 2009, 5: 717-720. 10.1098/rsbl.2009.0401.View ArticleGoogle Scholar
- Gill FB, Slikas B, Sheldon FH: Phylogeny of titmice (Paridae): II. Species relationships based on sequences of the mitochondrial cytochrome-B gene. Auk. 2005, 122: 121-143. 10.1642/0004-8038(2005)122[0121:POTPIS]2.0.CO;2.View ArticleGoogle Scholar
- Alström P, Ericson PGP, Olsson U, Sundberg P: Phylogeny and classification of the avian superfamily Sylvioidea. Mol Phylogenet Evol. 2006, 38: 381-397. 10.1016/j.ympev.2005.05.015.View ArticlePubMedGoogle Scholar
- Persson O, Öhrström P: A new avian mating system: ambisexual polygamy in Penduline Tit Remiz pendulinus. Ornis Scand. 1989, 20: 105-111. 10.2307/3676876.View ArticleGoogle Scholar
- van Dijk RE, Szentirmai I, Komdeur J, Székely T: Sexual conflict over care in Penduline Tits: the process of clutch desertion. Ibis. 2007, 149: 530-534. 10.1111/j.1474-919X.2007.00679.x.View ArticleGoogle Scholar
- Franz D: Paarungsystem und Fortpflanzungstrategie der Beutelmeise Remiz pendulinus. J Ornithol. 1991, 132: 241-266. 10.1007/BF01640533.View ArticleGoogle Scholar
- Schleicher B, Hoi H, Valera F, Hoi-Leitner M: The importance of different paternity guards in the polygynandrous penduline tit (Remiz pendulinus). Behaviour. 1997, 134: 941-959. 10.1163/156853997X00241.View ArticleGoogle Scholar
- van Dijk RE, Brinkhuizen DM, Székely T, Komdeur J: Parental care strategies in Eurasian penduline tit are not related to breeding densities and mating opportunities. Behaviour.
- van Dijk RE: Sexual conflict over parental care in penduline tits. PhD. Bath. 2009, United Kingdom: University of BathGoogle Scholar
- Dean WRJ: Cape penduline tit Anthoscopus minutus. Roberts Birds of Southern Africa. Edited by: Hockey PAR, Dean WRJ, Ryan PG. 2005, Cape Town, South Africa: John Voelker Bird Book Fund, 736-737.Google Scholar
- Harrap S, Quinn D: Tits, Nuthatches & Treecreepers. 1996, London, United Kingdom: Christopher HelmGoogle Scholar
- Catchpole CK, Slater PJB: Bird song: Biological themes and variations. 2007, Cambridge: Cambridge University PressGoogle Scholar
- Kingma SA, Szentirmai I, Székely T, Bókony V, Bleeker M, Liker A, Komdeur J: Sexual selection and the function of a melanin-based plumage ornament in polygamous penduline tits Remiz pendulinus. Behav Ecol Sociobiol. 2008, 62: 1277-1288. 10.1007/s00265-008-0556-x.View ArticleGoogle Scholar
- Andersson M: Sexual selection. 1994, Princeton, New Jersey: Princeton University PressGoogle Scholar
- Pogány Á, Székely T: Female choice in the penduline tit Remiz pendulinus : the effects of nest size and male mask size. Behaviour. 2007, 144: 411-427. 10.1163/156853907780756021.View ArticleGoogle Scholar
- Kingma SA, Szentirmai I, Székely T, Bókony V, Bleeker M, Liker A, Komdeur J: Sexual selection and the function of melanin-based plumage ornament in promiscuous penduline tits Remiz pendulinus. Behavioral Ecol Sociobiol. 2008, 62: 1277-1288. 10.1007/s00265-008-0556-x.View ArticleGoogle Scholar
- Valera F, Hoi H, Schleicher B: Egg burial in penduline tits, Remiz pendulinus : Its role in mate desertion and female polyandry. Behav Ecol. 1997, 8: 20-27. 10.1093/beheco/8.1.20.View ArticleGoogle Scholar
- van Dijk RE, Szentirmai I, Komdeur J, Székely T: Sexual conflict over parental care in Penduline Tits Remiz pendulinus : the process of clutch desertion. Ibis. 2007, 149: 530-534. 10.1111/j.1474-919X.2007.00679.x.View ArticleGoogle Scholar
- Persson O, Öhrström P: A new avian mating system: ambisexual polygamy in the Penduline Tit Remiz pendulinus. Ornis Scand. 1989, 20: 105-111. 10.2307/3676876.View ArticleGoogle Scholar
- Houston AI, Székely T, McNamara JM: Conflict over parental care. Trends Ecol Evol. 2005, 20: 33-38. 10.1016/j.tree.2004.10.008.View ArticlePubMedGoogle Scholar
- McGraw L, Székely T, Young LJ: Pair bonds and parental behaviour. Social behaviour: genes, ecology and evolution. Edited by: Székely T, Moore AJ, Komdeur J. Cambridge: Cambridge University Press
- Burley N: Sexual selection for aesthetic traits in species with biparental care. Am Nat. 1986, 127: 415-445. 10.1086/284493.View ArticleGoogle Scholar
- Sheldon BC: Differential allocation: tests, mechanisms and implications. Trends Ecol Evol. 2000, 15: 397-402. 10.1016/S0169-5347(00)01953-4.View ArticlePubMedGoogle Scholar
- Crudgington HS, Siva-Jothy MT: Genital damage, kicking and early death. The battle of the sexes takes a sinister turn in the bean weevil. Nature. 2000, 407: 855-856. 10.1038/35038154.View ArticlePubMedGoogle Scholar
- Arnqvist G, Rowe L: Correlated evolution of male and female morphologies in water striders. Evolution. 2002, 56: 936-947.View ArticlePubMedGoogle Scholar
- Arnqvist G, Nilsson T: The evolution of polyandry: multiple mating and female fitness in insects. Anim Behav. 2000, 60: 145-164. 10.1006/anbe.2000.1446.View ArticlePubMedGoogle Scholar
- Fiumera AC, Dumont BL, Clark AG: Natural variation in male-induced 'cost-of-mating' and allele-specific association with male reproductive genes in Drosophila melanogaster. Phil Trans R Soc Lond B. 2006, 361: 355-361. 10.1098/rstb.2005.1791.View ArticleGoogle Scholar
- Holland B, Rice WR: Perspective: Chase-away sexual selection: Antagonistic seduction versus resistance. Evolution. 1998, 52: 1-7. 10.2307/2410914.View ArticleGoogle Scholar
- Krüger O, Sorenson MD, Davies NB: Does coevolution promote species richness in parasitic cuckoos?. Proc R Soc B. 2009, 276: 3871-3879. 10.1098/rspb.2009.1142.PubMed CentralView ArticlePubMedGoogle Scholar
- Westneat DF, Sih A: Sexual conflict as a partitioning of selection. Biol Letters. 2009, 5: 675-677. 10.1098/rsbl.2009.0195.View ArticleGoogle Scholar
- Kvarnemo C, Ahnesjö I: The dynamics of operational sex ratios and competition for mates. Trends Ecol Evol. 1996, 11: 404-408. 10.1016/0169-5347(96)10056-2.View ArticlePubMedGoogle Scholar
- Owens IPF: Male-only care and classical polyandry in birds: phylogeny, ecology and sex differences in remating opportunities. Phil Trans Roy Soc Lond B. 2002, 357: 283-293. 10.1098/rstb.2001.0929.View ArticleGoogle Scholar
- Kokko H, Rankin DJ: Lonely hearts or sex in the city? Density-dependent effects in mating systems. Phil Trans Roy Soc Lond B. 2006, 361: 319-334. 10.1098/rstb.2005.1784.View ArticleGoogle Scholar
- Head ML, Lindholm AK, Brooks R: Operational sex ratio and density do not affect directional selection on male sexual ornaments and behaviour. Evolution. 2008, 62: 135-144. 10.1111/j.1558-5646.2007.00277.x.View ArticlePubMedGoogle Scholar
- Kempenaers B, Verheyen GR, Dhondt AA: Mate guarding and copulation behaviour in monogamous and polygynous blue tits: do males follow a best-of-a-bad-job strategy?. Behav Ecol Sociobiol. 1995, 36: 33-42. 10.1007/BF00175726.View ArticleGoogle Scholar
- Birkhead TR: Sperm competition in birds. Reviews of reproduction. 1998, 3: 123-129. 10.1530/ror.0.0030123.View ArticlePubMedGoogle Scholar
- Møller AP, Ninni P: Sperm competition and sexual selection: a meta-analysis of paternity studies of birds. Behav Ecol Sociobiol. 1998, 43: 345-358. 10.1007/s002650050501.View ArticleGoogle Scholar
- Harvey P, Pagel MD: The comparative method in evolutionary biology. 1991, Oxford: Oxford University PressGoogle Scholar
- Freckleton RP, Harvey PH, Pagel M: Phylogenetic analyses and comparative data: a test and review of evidence. Am Nat. 2002, 160: 712-726. 10.1086/343873.View ArticlePubMedGoogle Scholar
- Thomas GH, Székely T: Evolutionary pathways in shorebird breeding systems: Sexual conflict, parental care, and chick development. Evolution. 2005, 59: 2222-2230.View ArticlePubMedGoogle Scholar
- Cramp S, Perrins CM, Brooks DM: Handbook of the birds of Europe, the Middle East and North Africa - The Birds of the Western Palearctic. 1993, Oxford: Oxford University Press, 7:Google Scholar
- van Dijk RE, Pilon AE, Szentirmai I, Székely T: The influence of habitat structure on sexual conflict over care in penduline tits. Ardea. 2008, 96: 3-11.View ArticleGoogle Scholar
- Cohen J: Statistical power analysis for the behavioral sciences. 1988, Hillsdale, NJ: Lawrence Earlbaum Associates, 2Google Scholar
- Lehmann EL: Nonparametrics. Statistical methods based on ranks. 1975, San Francisco: Holden-DayGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.