The imprint of the Slave Trade in an African American population: mitochondrial DNA, Y chromosome and HTLV-1 analysis in the Noir Marron of French Guiana
© Brucato et al; licensee BioMed Central Ltd. 2010
Received: 25 February 2010
Accepted: 19 October 2010
Published: 19 October 2010
Retracing the genetic histories of the descendant populations of the Slave Trade (16th-19th centuries) is particularly challenging due to the diversity of African ethnic groups involved and the different hybridisation processes with Europeans and Amerindians, which have blurred their original genetic inheritances. The Noir Marron in French Guiana are the direct descendants of maroons who escaped from Dutch plantations in the current day Surinam. They represent an original ethnic group with a highly blended culture. Uniparental markers (mtDNA and NRY) coupled with HTLV-1 sequences (env and LTR) were studied to establish the genetic relationships linking them to African American and African populations.
All genetic systems presented a high conservation of the African gene pool (African ancestry: mtDNA = 99.3%; NRY = 97.6%; HTLV-1 env = 20/23; HTLV-1 LTR = 6/8). Neither founder effect nor genetic drift was detected and the genetic diversity is within a range commonly observed in Africa. Higher genetic similarities were observed with the populations inhabiting the Bight of Benin (from Ivory Coast to Benin). Other ancestries were identified but they presented an interesting sex-bias. Whilst male origins spread throughout the north of the bight (from Benin to Senegal), female origins were spread throughout the south (from the Ivory Coast to Angola).
The Noir Marron are unique in having conserved their African genetic ancestry, despite major cultural exchanges with Amerindians and Europeans through inhabiting the same region for four centuries. Their maroon identity and the important number of slaves deported in this region have maintained the original African diversity. All these characteristics permit to identify a major origin located in the former region of the Gold Coast and the Bight of Benin; regions highly impacted by slavery, from which goes a sex-biased longitudinal gradient of ancestry.
The genetic dispersal that occurred during the Slave Trade remains complex, due to the large diversity of populations involved. Modern African American populations show this genetic diversity, inherited from African, European and Amerindian populations. Nine to ten million Africans were deported to the American colonies during the 16th-19th centuries. In the Guianese regions , between Brazil and Venezuela, approximately 400,000 slaves arrived to work in the plantations from 1604 to 1815 for mainly Dutch, but also English, French and Portuguese Jew settlers. Although many of these slaves were shipped by the Dutch West Indische Compagnie (WIC, 1620-1674), which controlled several African trading posts notably in the Bight of Benin, most of them were sold by independent slavers who traded along the Atlantic coast of Africa. Historical reports show widespread origins of the enslaved working force in Guiana, ranging over the Atlantic African coast from Senegal to Angola: 12% of the slaves came from Senegambia (present-day Senegal, Guinea-Bissau, Guinea), Sierra Leone and the Windward Coast (present-day Liberia and a part of the Ivory Coast), 56% from the Gold Coast (the remaining part of the Ivory Coast and Ghana) and the Bight of Benin (present-day Togo, Benin and a part of Nigeria), 5% from the Bight of Biafra (the remaining part of present-day Nigeria, Cameroon, Equatorial Guinea and Gabon), and 28% from South West Africa (present-day Angola) . The slavers took advantage of this high cultural diversity, by breaking all ethnic and familial networks and by maintaining a sex-ratio (2/3 men and 1/3 women) to prevent rebellions during the Middle Passage (the crossing of Atlantic Ocean) and in the plantations.
Despite these precautions, many slaves managed to escape from European oppression once on American soil, a phenomenon known as marooning. In Dutch Guiana, these maroons took refuge in the equatorial Amazonian forest, and reconstructed entire communities known as the Noir Marron (or Bushinengué) . These escapes were favoured by conflicts between the Dutch and French, as was observed during the French embargo of Dutch Guiana in 1712. They rapidly adapted themselves to this new environment, to represent a real threat for the colonial power, forcing the government to sign peace treaties. Between 1760 and 1809, six Noir Marron communities were officially recognized: Saramaka, Ndjuka, Aluku, Paramaka, Matawai and Kwinti . From the beginning of the 19th century to 1986 and the Surinamese civil war, they were often evicted from their territories and many of them were forced to take refuge in French Guiana. Despite these fluctuating conditions of life, the Noir Marron communities have prospered and their culture has asserted itself. Although the maroon identity is their cornerstone, cultural exchanges with Europeans and Amerindians have been intense. Large influences are detectable, for example, in their languages composed of English, Dutch, Portuguese or Arawak words, and in the structure of their villages, which are inherited from Amerindian knowledge. Today, living in Surinam and French Guiana, 50,000 individuals constitute one of the last known American maroon society , and contribute to the large ethnic diversity of African American populations.
The complex variety of African American communities has been well-studied, notably with genetic data revealing a high diversity among them. These populations can be highly admixed, as an Afro-Brazilian group from the State of Saõ Paulo whose origins are at: 26% African, 62% European and 12% Amerindian . They can have a balanced admixed profile, as the Brazilian community of Cameta: 53% African, 24% European and 23% Amerindian . But some of them show a relatively preserved African ancestry, as the Gullah/Geechee in United States: 96% African and 3% European and 1% Amerindian . Furthermore, these studies have shown that gene-flow within each group is often sex-biased, adding another level of complexity, as in the Afro-Venezuelian community of Birongo which has a maternal African inheritance of 100% and a paternal European ancestry of 93% . Concerning the Noir Marron population, a preliminary anthropological study on the Gm allotypic system showed a high degree of conservation of the African genetic contribution (95.1%) and very low levels of European (2.6%) and Amerindian (1.7%) contributions. This contradicts expectations based on their cultural exchanges and emphasises the importance of the maroon identity which has shaped their profile of admixture . Moreover, this seems to constitute an original genetic characteristic in comparison with other African American populations inhabiting Latin America. Although the Gm system has a high power of discrimination between continental populations, it is limited for finer-scale analyses . These original findings have to be confirmed by more powerful tools. Consequently, three genetic markers have been analysed in the present study, to obtain a better picture of the genetic structure of the Noir Marron. Uniparental lineages were determined through the analysis of mitochondrial DNA (mtDNA) and non-recombinant Y chromosome (NRY) haplotypes. Through the large number of African American and African populations typed for these systems, updated by original data from the Ivory Coast and Benin, the data available are informative to identify the ancestry of each haplotype observed [9, 10]. A third particular genetic system, the HTLV-1 retrovirus, has been explored because of its interest in human populations [11, 12]. Due to its ability to integrate itself in human genome, its low mutation rate and its mode of transmission (mainly mother-to-child), it represents a relevant marker for infected groups. For some genotypes and especially concerning the Long Terminal Repeat (LTR) region, the phylo-geographical tree is highly discriminative . This study is the first to combine these three genetic systems to study an African American population. In obtaining a better understanding of the genetic structure of the Noir Marron in French Guiana, the aim of this study is dual: (i) to confirm or not the highly preserved African gene pool obtained from the Gm allotypic system, which contradicts the cultural exchanges between the Noir Marron, Amerindians and Europeans, and (ii) to retrace the origin of this community by estimating the contribution of the historical African areas of slavery.
Noir Marron from French Guiana
This region was extensively used for slaves after the 18th century. Belonging to the historical areas of the Windward Coast and the Gold Coast, approximately 700,000 Africans living in this region were deported to the Americas during the Triangular Trade . Today, about 60 ethnic groups constitute the cultural diversity of the Ivory Coast. Among them, blood samples from 128 Ahizi and 62 Yacouba were collected filling a lack in the available databases. All samples were obtained with the informed consent of the participants. The locations of these two populations are shown on Figure 2.
Maternal lineages were determined by a two-step procedure. The entire HVS-I (positions 16012-16400), the intermediate region (positions 16401-72) and part of HVS-II (positions 73-263) of the mtDNA control region were sequenced following the protocol described previously . A first alignment step with rCRS  was made using BioEdit v.22.214.171.124, to determine a preliminary haplogroup. Twenty-seven informative Single Nucleotid Polymorphisms (SNPs) on the coding region (positions 10873, 13276, 13789, 13470, 10810, 12950, 3594, 13710, 10400, 9818, 11899, 12049, 14088, 13485, 14034, 13803, 15939, 4158, 3693, 13958, 10086, 8618, 14905, 4218, 2352, 750, 7851) were typed by minisequencing SNaPshot® (PE, Applied Biosystems). All data were obtained on an ABI PRISM 3730 sequencer (PE, Applied Biosystems). The final haplogroup assignment was obtained by the most recent mtDNA phylogeny .
NRY were determined by two types of markers. Seventeen Short tandem repeats (STRs) were typed using the AmpFLSTR Yfiler® kit (PE, Applied Biosystems). Twenty-four Unique Event polymorphisms (UEPs; SRY 10831, M213, M9, M70, M22, Tat, 92R7, M173, P25, M96, M35, M78, M81, M123, M34, M17, M18, M73, M37, M63, M126, M153, M160, SRY 2627) were typed following the protocol published previously . An additional set of nine UEPs (M33, U174, M191, M75, U209, M2, P2, M91, M60) has been designed to precise the haplogroup assignment. All data were read on an ABI PRISM 3730 sequencer and analysed with Genemapper v.4.0 (PE, Applied Biosystems). The YAP analysis has been obtained following Hammer and Horai . The haplogroup assignment follows the most recently updated NRY phylogeny .
The overall HTLV-I seroprevalence in the Noir Marron population in French Guiana is about 6% in women and 4% in men [13, 22–25]. Determination of HTLV-1 genomic subtype in env and LTR regions, which are used as markers of the migration of infected populations, was performed for 23 samples of HTLV-1 infected Noir Marron individuals representative of the main Noir Marron communities: Saramaka (n = 6), Ndjuka (n = 6), Aluku (n = 5) and Paramaka (n = 6) (Genbank accession number: GU725032-GU725054) and compared with the corresponding database available in Genbank.
High molecular-weight DNA was extracted from peripheral blood buffy-coats using the QIAamp DNA Blood Mini Kit (Qiagen GmbH, Hilden, Germany). All samples were firstly determined to contain amplifiable DNA after being amplified by PCR for human β-globin. Five hundred nanograms, quantified by spectrophotometry, of each DNA sample was then subjected to two series of PCR to obtain the complete long terminal repeat (LTR) (755-bp) and a 522-bp region of the env gene, as previously described [26, 27]. To prevent false-positive reactions, all pre- and post-PCR operations were performed in separate facilities. The complete LTR was obtained for eight individuals while the 522-bp Env fragment was obtained for the 23 samples tested. The amplified products of the appropriate size were cloned, sequenced and phylogenetically analysed as described [28, 29].
All summary statistics for mtDNA and NRY haplotype variation, Tajima's D and Fu's Fs tests were calculated using the ARLEQUIN 3.11 software package . Four databases were compiled from published studies, updated with data from the African populations of Benin and Ivory Coast analysed in the present study. The African mtDNA database was composed of 170 populations representing 8727 HVS-I and 3500 HVS-II haplotypes associated with their corresponding haplogroup assignment. The African NRY database was composed of 145 populations representing 8909 individuals typed for UEP informative for the haplogroup assignment and 1200 Y-STR profiles. As the Y- SNP haplogroup information is lacking in some African regions, it was statistically inferred from the Y-STR data available, as previously explained . For some analyses, the African databases were divided into nine groups according to their historical region of slavery described by H.S. Klein , the genetic coherence and published genetic studies [2, 31]: North Africa (Algeria, Canary Island, Egypt, Mauritania, Morocco), Windward Coast, Senegambia and Sierra Leone (Cabo Verde, Guinee-Bissau, Mali, Senegal, Sierra Leone), Gold Coast and Bight of Benin (Benin, Burkina Faso, Ivory Coast), Bight of Biafra (Cameroon, Central African Republic, Chad, Equatorial Guinea, Gabon, Nigeria, Niger, Sao Tome), South West Africa (Angola, Cabinda, Democratic Republic of Congo), South Africa (Botswana, Malawi, Namibia, Zambia, Zimbabwe), South East Africa (Mozambique), East Africa (Ethiopia, Kenya, Rwanda, Somalia, Uganda, Sudan, Tanzania), and Pygmies. The mtDNA and NRY databases of African American and urban hybridised populations were composed of 95 and 90 populations, respectively. Due to the heterogeneous resolution of haplogroup assignment among populations, forming a non-relevant database, only the admixture rates of continental populations (European, Amerindian and African), given in published studies, have been considered. Despite this bias, as African ancestry is relatively common in all African Americans [1, 32], their discrimination is mostly due to differential contribution of non-African gene pools. All populations considered in the present study are located on Figure 2 and complete references are available in Additional file 1.
Haplotype networks were generated for mtDNA haplogroups L2a* and L1c*, and for the NRY haplogroup E1b1a* via the median-joining algorithm of Network v.126.96.36.199 http://www.fluxus-engineering.com from the Noir Marron data and all African and African American comparable data. To obtain the most parsimonious networks the reticulation permissivity was set to zero. Data were pre-processed using the star contraction option in Network v.188.8.131.52 . For the mtDNA data, hypermutable sites were identified by post-processing using the Steiner (MP) algorithm within Network 184.108.40.206, and removed from the analysis . Because of the high level of reticulation in the E1b1a* sample, Y-STR loci were subdivided into three mutation rate classes based on observed STR allelic variance and weighted as follows: 4 (low) for DYS391, DYS392; 2 (intermediate) for DYS389I, DYS389II, DYS19, DYS393, DYS390; or 1 (high) for DYS385a/b .
Cross-population comparisons of maternal and paternal lineages based on the frequency of haplogroups or rates of continental ancestry common to all samples in the database were performed using ARLEQUIN 3.11 . The significance of Fst values is given for p-values under a threshold of 0.05. All results obtained for the comparison between the Noir Marron and each population of the database were graphically plotted on a map using Surfer v.8.0, using the location of each population given in the corresponding study. Factorial Correspondence Analysis (FCA) based on mtDNA and Y-chromosome haplogroup frequencies were performed using XLstat v.7.5.2. Analyses of molecular variance (AMOVA) were performed with ARLEQUIN 3.11 . Admixture estimates were calculated by two different methods. The first, based on haplotypic homology (up to 99% of homology), was calculated by the percentage of shared lineages (LS) between the Noir Marron and each compared group . Haplotype comparisons were performed from HVS-I mtDNA sequences (16030-16360) and NRY core haplotypes (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b) to obtain the most relevant results from the compiled databases. The second estimator, mY, was calculated with the ADMIX2.0 program . Both mtDNA-based and NRY-based estimates were calculated from haplogroup frequencies without taking into account molecular distances between haplogroups. The parental populations were chosen among the groups that presented a Fst value, obtained by the AMOVA for the comparison with the Noir Marron, lower than an threshold fixed at 0.1.
Concerning HTLV-1 Env and LTR phylogenetic analysis, the phylogenetic trees were generated using the Neighbor-Joining method performed in the PAUP v.4.0b10 program using representative HTLV-1 sequences available in Genbank, including four env sequences and one LTR sequence typed in Noir Marron individuals of French Guiana . The strains were aligned with the DAMBE v.4.2.13 program and the final alignment was submitted to the Modeltest v. 3.6 program to select, according to the Akaike Information Criterion (AIC), the best model to apply to phylogenetic analyses. Confidence levels were estimated with the distance NJBOOT program (1,000 replicates).
Summary statistics estimating the mtDNA genetic diversity of the Noir Marron.
(9.402 - 169.084)
(38.367 - 96.897)
(2.907 - 29.191)
(14.525 - 55.171)
Total Noir Marron
(51.408 - 103.439)
Thus, the mtDNA pattern of the Noir Marron is closer to that of Africans than of most African Americans. This maternal African ancestry showed a low divergence with 41% of African groups (Fst < 0.05). Many of them are located in West Africa and South West Africa, such as Senegal , Cabo Verde , Sierra Leone , Guinea-Bissau , Mali , Ivory Coast (present study), Burkina Faso , Benin  (present study), Niger , Cameroon [58–63], Chad , Gabon , Equatorial Guinea , Angola  and Cabinda . However, inside this large area, three clusters of high genetic divergence from the mtDNA gene pool were observed identifying the Pygmy communities of Cameroon [61, 62], Gabon  and Central African Republic [63, 67], the Fang from Equatorial Guinea  and the Mbuti from the Democratic Republic of Congo . High divergences were also present in North African, East African, South East African and South African populations, even though some groups have low differences, as one Somalian population , one Sudanese population , one Namibian population , one Rwandan group  and three Bantu-speaking groups located in the Democratic Republic of Congo , Uganda  and Kenya .
Estimators of shared maternal ancestries of the Noir Marron.
Windward Coast, Senegambia and Sierra Leone
Gold Coast Bight of Benin
Bight of Biafra
South West Africa
South East Africa
Fst (versus Noir Marron)
Shared Lineages rate (LS)
Admixture rate (mY)
0 (± 0.17)
0.64 (± 0.20)
0.13 (± 0.16)
0.23 (± 0.17)
0 (± 0.18)
All mtDNA data of the five African groups presenting Fst values lower than the chosen threshold (Fst < 0.1) were used to evaluate the maternal African admixture percentage of the Noir Marron community (Table 2). Both estimators, LS and mY rates, identify a major origin in the "Gold Coast and Bight of Benin" (LS = 29%; mY = 64%). Two other important ancestries are detected in "South West Africa" (LS = 26%; mY = 23%) and in the "Bight of Biafra" (LS = 19%; mY = 13%). The two last regions present lower probabilities of ancestry: "Windward Coast, Senegambia and Sierra Leone" (LS = 13%; mY = 0%) and "South East Africa" (LS = 14%; mY = 0%).
The NRY gene pool of the Noir Marron showed low Fst values with only 10% of the African groups; the majority located in the Ivory Coast (present study), Ghana , Benin (present study) and in Cameroon [82, 83], and with one from Gabon , Angola , Namibia , Zimbabwe  and South Africa . The other groups: North Africans, East Africans, South East Africans, Pygmies [76, 77, 82] and other South Africans, notably the Khoisan groups [76, 82], presented high divergence (Fst>0.25). The paternal ancestry of the Noir Marron is observed to be less widespread than the maternal ancestry.
Estimators of shared paternal ancestries of the Noir Marron.
Windward Coast, Senegambia and Sierra Leone
Gold Coast Bight of Benin
Bight of Biafra
South West Africa
South East Africa
Fst (versus Noir Marron)
Shared Lineages rate (LS)
Admixture rate (mY)
0.26 (± 0.28)
0.74 (± 0.19)
0 (± 0.35)
0 (± 0.20)
0 (± 0.15)
All NRY data of the five African groups presenting Fst values lower than the chosen threshold (Fst < 0.1) were used to evaluate the paternal African admixture percentage of the Noir Marron community (Table 3). As for mtDNA analysies, both estimators, LS and mY rates, identify a major origin in the "Gold Coast and Bight of Benin" (LS = 28%; mY = 74%). One other non negligible ancestry is located in "Windward Coast, Senegambia and Sierra Leone" (LS = 25%; mY = 26%). The remaining regions show lower probabilities of ancestry: "Bight of Biafra" (LS = 19%; mY = 0%) "South West Africa" (LS = 13%; mY = 0%).
The analysis of the env 522-bp fragments of the 23 new HTLV-1 Noir Marron strains indicated neither deletion nor insertion compared with the ATK-1 reference strain. Comparison of the 23 strains indicated that four sequences and two pairs were identical. All together, the 23 strains showed a nucleotidic interstrain difference ranging from 0-1.5% and an amino acid divergence ranging from 0-1.7%. Nevertheless, 17 strains were closely related with 0-0.8% of nucleotide differences on 522 nucleotides and 0-1.7% at the amino acid level.
In parallel, LTR sequences obtained from eight representative individuals of the four Noir Marron communities: Saramaka (2), Ndjuka (2), Aluku (2) and Paramaka (2) were analysed and exhibited 0-2.4% divergence at the nucleotide level. Interestingly, six strains were closely related with only 0-0.7% nucleotidic divergence for the 757-bp LTR fragment.
An original African American population
The African American community is composed of highly diverse ethnic groups in terms of their historical, cultural and biological inheritances. Each of them has their specific identity. Being one of the last known American maroon community, the Noir Marron are an important part this diversity . A previous analysis of the Gm system showed their preserved African gene pool (>95%), revealing a first insight into their genetic originality in comparison with other neighbouring African American groups . The present study, exploring three different genetic systems, strengthens this conclusion. Although each parental lineage presents European and/or Amerindian contribution, the bulk (>95%) of their genetic diversity is inherited from African ancestors. Indeed, 99.3% of the mtDNA genetic profile of the Noir Marron comes from Africa, whereas the paternal African ancestry increases to 97.6% and the HTLV-1 strains, probably of African origin, represent 6/8 for the LTR and 20/23 for the env region. Thus, despite four centuries in America, neighboured by European settlers and Amerindian ethnic groups with whom the Noir Marron made cultural exchanges , none or restricted gene flow has influenced their African genetic identity. This emphasises the role of the maroon identity in shaping their genetic profile. Although exchanges were necessary for the survival of the community, the ethnic integrity of the maroon society is characterised by a struggle against foreign threats, and above all the struggles of former slavers. Thus, interethnic couples, which effectively exist as between the Aluku and the Wayana , would contradict this cultural rule. This does not mean that the Noir Marron have been genetically isolated in the equatorial forest as revealed by their genetic diversity (HmtDNA = 0.988; HNRY = 0.990). Comparable to the values of African populations, these values reflect the mass arrival of slaves into the Guianas over four centuries, an important proportion of which increased the number of the Noir Marron. Genetic diversities do not seem to have been impacted by demographic crises or geographical isolations which could have occurred during the history of Noir Marron which was punctuated by many conflicts (D = -0.55 (0.357); Table 1). Although some communities were briefly reduced to a hundred individuals, as the Aluku were , the constant flow of new maroons balanced the genetic lost (Figures 3 and 6). Forming sustainable groups, still increasing in number, their adaptation to the Guianese context has been facilitated. Their maroon identity, their adaptation to the Amazonian environment and the relatively important number of immigrants explain why the Noir Marron, who today constitute 30,000 individuals in French Guiana, have conserved their African genetic inheritance.
These characteristics are all the more interesting when compared with other African American groups. The composition of the uniparental haplogroups of this African gene pool is close to observations in other African American populations, notably because of the high frequencies of L2a (22,5%) and L1c (19%) for the maternal lineage and E1b1a (88%) for the paternal lineage (Figures 3 and 6), such in African American in the United States (L1c = 11%; L2a = 19%; E1b1a = 62%) or in Afro-Brazilian in Porto Alegre (L1c = 14%; L2a = 17%; E1b1a = 44%) [40, 46, 84]. As most of the slavers traded along the Atlantic coast of Africa, the average composition of each lineage is similar among the African American group. If this ancestral genetic pool was effectively common, the evolution of each one has produced highly divergent patterns. The population pairwise Fst comparisons of both uniparental systems showed that the majority of African American groups presented significant genetic differences from the Noir Marron (Fst>0.25; Figures 4 and 7). The discrepancies are mainly due to European and/or Amerindian contributions, which are most often sex-biased . This preferential gene flow is partly due to the importance of European male migration and an easier acceptance of Amerindian women in tri-hybridised communities . The African inheritance of the Noir Marron, consequently not sex-biased, is peculiar in the African American landscape. Even their neighbouring African American communities are much more hybridised, as observed in North East Brazil [5, 47, 48, 50, 79–81]. If this characteristic is mainly shaped by their maroon identity, it is surprising to notice that other maroon groups present an admixed and sex-biased genetic profile, such as the Maroons in Jamaica (African maternal lineage: 87%; African paternal lineage: 58%) . Even the Curiau quilombo, which could have originated from the same plantations as the Noir Marron, present a highly hybridised genetic profile (African maternal lineage: 54%; African paternal lineage: 37%) . Furthermore, most maroon groups have lost a part of their genetic diversity after genetic drift due to their relative isolation, as has occurred in the Garifuña in Honduras (HmtDNA = 0.897)  and in the Angolares in the African island of São Tomé (HmtDNA = 0.919) [88, 89]. Thus, the Noir Marron are also genetically original within the maroon populations cluster. The reason for this difference can be enlightened by examining the groups presenting similar patterns in both lineages (Fst < 0.15). They are all located on the East coast of the United States, such as the Gullah/Geechee in South Carolina [43, 78]. Although these groups never present an estimated African ancestry as high as in the Noir Marron, they present a low sex-bias. A historical cultural phenomenon can be evoked to link this common pattern. Although assimilation of Amerindian and African women was encouraged as a strategy for the occupation in Portuguese colonies , tolerated in Spanish territories , it was not the case in French, English and Dutch colonies [91, 92]. Such cultural traits have favoured inter-ethnic admixture in the first case and separated communities in the second. The common behaviours in these colonies have caused a low level of sex-biased admixture, bringing the gene pools of the Noir Marron and the African Americans of the East coast of the United States closer. The combination of the maroon identity in the former Dutch colony has preserved the African genetic diversity of the Noir Marron ancestors.
Retracing the African origin
The exceptional conservation of the African genetic diversity allows a relevant picture of the genetic inheritance of slaves that were deported to the Guianas to be determined. If the African inheritance is genetically marked by the predominance of L* haplogroup in maternal lineages, E1b1* in paternal lineages and HTLV-1 A strains of African origin, the detailed examination of each of these components gives a more precise location of their ancestries [31, 76]. Indeed, a close link with West African populations, also suggested by the Gm system analysis , is identifiable through the relatively high frequencies of some mtDNA sub-haplogroups as L2a1 (22.5%), L1b (14.1%), L3d (3.5%), L3f1 (3.5%), NRY sub-haplogroups as E1b1a7* (33.3%) and E1b1a8* (21.4%) [31, 76]. Most of the HTLV-1 Noir Marron strains analysed were clustered with West African strains originating from the Ivory Coast, Ghana, Senegal and Burkina-Faso (Figure 9). Secondly, genetic relationships between the Noir Marron and South West African populations can be distinguished. Although no HTLV-1 B and D subtypes (Central African genotypes) were detected among the studied samples, the observed frequencies of mtDNA sub-haplogroups L1c1 (12.7%) and L3e2 (7.4%), and the NRY sub-haplogroup B (4.7%) [9, 31, 62] sign the link between the Noir Marron and South West Africa. These two genetic affinities were confirmed by cross-comparisons between the gene pools of the Noir Marron and each African population (Figures 4 and 7). The ethnic groups inhabiting West and South West Africa presented the lowest genetic divergence (Fst < 0.15) compared with other African groups, at the noticeable exception of the Pygmy communities, the Fang and some groups from Senegal, Chad and Niger. North Africans, East Africans and Khoisans (Fst>0.25) have not contributed to the Noir Marron gene pool, confirming that the presence of NRY haplogroups A and B are probably of South West African inheritance. The West and South West African mixed-ancestry of the Noir Marron is attested by FCA performed for each lineage, plotting the Noir Marron diversity inside a cluster of these populations (Figures 5 and 8). As hypothesised, the populations located in the historical regions of slavery are the most probable Noir Marron ancestors.
Despite the fact that the relative genetic homogeneity within these populations make any grouping non-significant, as reported in many studies [41, 93], historical data give a strong significance to the clustering because of their importance during the Atlantic Slave Trade. All estimators of ancestry, for both uniparental systems, state the Gold Coast and the Bight of Benin as the major origin of the Noir Marron's ancestors (mtDNA: LS = 0.29; mY = 0.64; NRY: LS = 0.28; mY = 0.74; Tables 2 and 3). A region also suggested by the phylogenetic analyses of the HTLV-1 strains (Figure 9 and Additional file 5). These converging results are concordant with historical records showing that this region was the port of departure of slaves who were deported to the Guianas . Almost 224,000 individuals, natives of the Gold Coast and the Bight of Benin, were sold as slaves to work in the Guianese plantations. Their relative importance has largely imprinted the genetic background of the Noir Marron but also their culture. For example, Akan words are still present in their vocabulary; their pantheon is largely inherited from those present in populations in Ghana; funeral rites are common with Fanti-Ashanti customs . Thus, the Noir Marron culture and genes have kept the traces of individuals coming from the Gold Coast and the Bight of Benin, a region largely impacted by the Atlantic Slave Trade.
As highlighted by historical data, slaves never came from a unique region and the Noir Marron gene pool has also kept this characteristic. Indeed, other ancestries are detected in uniparental systems, in a sex-biased manner, contradictory to the Gold Coast and the Bight of Benin origin. The majority of the remaining maternal ancestry is located in the Bight of Biafra (LS = 0.19; mY = 0.13; Tables 2) and in South West Africa (LS = 0.28; mY = 0.23; Tables 2), while the largest part of the remaining paternal ancestry is located in the region of the Windward Coast, Senegambia and Sierra Leone (LS = 0.25; mY = 0.26; Tables 3). Thus, a sex-biased ancestry is detected in the Noir Marron gene pool. From a major origin in the Gold Coast and the Bight of Benin, a paternal gradient goes north, while a maternal gradient goes south. This divergent geographical gradient of the uniparental ancestries may be explained by regional-specific characteristics of trading during the Atlantic Slave Trade. In the Windward Coast, Senegambia and Sierra Leone, slave trade was also implied in the Trans-Saharan Slave Trade  in which women were more sold than men, reducing the number of women sent to the Americas. Men from these regions were judged by European settlers to be more robust than Angolans to work in the plantations, raising the demand of male slaves in these trading posts, such as in the Gorée Island. The maternal gradient, more surprisingly, may be the consequence of these practices. The rising price of male slaves from the Windward Coast, Senegambia and Sierra Leone would have forced the slavers to balance the cost of buying slaves in the south, where slave markets were created for the Atlantic Slave Trade, without competition from the Arab traders. Thus, in the Bight of Biafra and South West Africa, slavers could have bought more women to maintain a sex-ratio close to 2/3 men and 1/3 women .
Belonging to the wide African American cultural area, the Noir Marron in French Guiana is unique due to their African gene pool. Despite four centuries neighboured by Europeans and Amerindians with whom intense cultural exchanges were made, their maroon identity has limited gene flow. The conservation of the African diversity in each genetic system studied revealed a probable non-altered inheritance from their slave ancestors. A major origin was located on the Gold Coast and in the Bight of Benin; regions highly impacted by slavery. From this region, uniparental genetic markers showed a sex-biased origin, with the remaining male ancestry located from Senegal to Benin, and the remaining female ancestry from the Ivory Coast to Angola. Different historical and cultural traits of the Slave Trade have created a differential migration of the female and male enslaved ancestors of the Noir Marron. Thus, this sex-biased African ancestry is still genetically imprinted in the Noir Marron gene pool, a characteristic that deserves to be examined in other African American groups, such as the Creoles, in order to gain a relevant picture of the dynamics of the African gene flow that occurred during the Slave Trade.
We are grateful to all the people who contributed to the collection of blood samples, notably André Chaventré and Gil Bellis (samples from Ivory Coast). We wish to acknowledge support from the CRGS platform of Genopole Toulouse Midi Pyrénées, where mtDNA and NRY sequencing/genotyping work was performed. We are grateful for the scientific support of the Service de Protection Maternelle et Infantile de Guyane and the Centre de Prévention et de Vaccination de St Laurent du Maroni (D. Gaquière et Dr. B. Fougère). This work was partially financed by the following research grants: Programme Interdisciplinaire CNRS "Amazonie - Analyse, modélisation et ingénierie des systèmes amazoniens"; The French Agence Nationale de Recherche (2006 Microbiologie-06MIME-017-01). We thank Dan Zadik and Patricia Balaresque for their help in the Y-haplogroup predictions inferred from STR data and Luis L Quintana-Murci for discussions and critical reading of the manuscript.
- Klein HS: The Atlantic Slave Trade. 1999, Cambridge: Cambridge University PressGoogle Scholar
- Eltis D, Behrendt SD, Richardson D, Klein HS: The Trans-Atlantic Slave Trade: A Database on CD-ROM. 1999, CambridgeGoogle Scholar
- Price R, Price S: Les Marrons en Guyane. Châteauneuf-le-Rouge: Vents d'Ailleurs. 2002Google Scholar
- Muniz YC, Ferreira LB, Mendes-Junior CT, Wiezel CE, Simoes AL: Genomic ancestry in urban Afro-Brazilians. Ann Hum Biol. 2008, 35 (1): 104-111. 10.1080/03014460701782256.View ArticlePubMedGoogle Scholar
- Bortolini MC, Da Silva WAJW, De Guerra DC, Remonatto G, Mirandola R, Hutz MH, Weimer TA, Silva MC, Zago MA, Salzano FM: African-derived South American populations: A history of symmetrical and asymmetrical matings according to sex revealed by bi- and uni-parental genetic markers. Am J Hum Biol. 1999, 11 (4): 551-563. 10.1002/(SICI)1520-6300(1999)11:4<551::AID-AJHB15>3.0.CO;2-Z.View ArticlePubMedGoogle Scholar
- Parra EJ, Kittles RA, Argyropoulos G, Pfaff CL, Hiester K, Bonilla C, Sylvester N, Parrish-Gause D, Garvey WT, Jin L, et al: Ancestral proportions and admixture dynamics in geographically defined African Americans living in South Carolina. Am J Phys Anthropol. 2001, 114 (1): 18-29. 10.1002/1096-8644(200101)114:1<18::AID-AJPA1002>3.0.CO;2-2.View ArticlePubMedGoogle Scholar
- Brucato N, Tortevoye P, Plancoulaine S, Guitard E, Sanchez-Mazas A, Larrouy G, Gessain A, Dugoujon JM: The genetic diversity of three peculiar populations descending from the slave trade: Gm study of Noir Marron from French Guiana. C R Biol. 2009, 332 (10): 917-926. 10.1016/j.crvi.2009.07.005.View ArticlePubMedGoogle Scholar
- Dugoujon JM, Hazout S, Loirat F, Mourrieras B, Crouau-Roy B, Sanchez-Mazas A: GM haplotype diversity of 82 populations over the world suggests a centrifugal model of human migrations. Am J Phys Anthropol. 2004, 125 (2): 175-192. 10.1002/ajpa.10405.View ArticlePubMedGoogle Scholar
- Karafet TM, Mendez FL, Meilerman MB, Underhill PA, Zegura SL, Hammer MF: New binary polymorphisms reshape and increase resolution of the human Y chromosomal haplogroup tree. Genome Res. 2008, 18 (5): 830-838. 10.1101/gr.7172008.PubMed CentralView ArticlePubMedGoogle Scholar
- van Oven M, Kayser M: Updated comprehensive phylogenetic tree of global human mitochondrial DNA variation. Hum Mutat. 2009, 30 (2): E386-394. 10.1002/humu.20921.View ArticlePubMedGoogle Scholar
- Gessain A, Gallo RC, Franchini G: Low degree of human T-cell leukemia/lymphoma virus type I genetic drift in vivo as a means of monitoring viral transmission and movement of ancient human populations. J Virol. 1992, 66 (4): 2288-2295.PubMed CentralPubMedGoogle Scholar
- Miura T, Fukunaga T, Igarashi T, Yamashita M, Ido E, Funahashi S, Ishida T, Washio K, Ueda S, Hashimoto K, et al: Phylogenetic subtypes of human T-lymphotropic virus type I and their relations to the anthropological background. Proc Natl Acad Sci USA. 1994, 91 (3): 1124-1127. 10.1073/pnas.91.3.1124.PubMed CentralView ArticlePubMedGoogle Scholar
- Plancoulaine S, Buigues RP, Murphy EL, van Beveren M, Pouliquen JF, Joubert M, Remy F, Tuppin P, Tortevoye P, de The G, et al: Demographic and familial characteristics of HTLV-1 infection among an isolated, highly endemic population of African origin in French Guiana. Int J Cancer. 1998, 76 (3): 331-336. 10.1002/(SICI)1097-0215(19980504)76:3<331::AID-IJC8>3.0.CO;2-W.View ArticlePubMedGoogle Scholar
- Plancoulaine S, Gessain A, Joubert M, Tortevoye P, Jeanne I, Talarmin A, de The G, Abel L: Detection of a major gene predisposing to human T lymphotropic virus type I infection in children among an endemic population of African origin. J Infect Dis. 2000, 182 (2): 405-412. 10.1086/315741.View ArticlePubMedGoogle Scholar
- Plancoulaine S, Gessain A, Tortevoye P, Boland-Auge A, Vasilescu A, Matsuda F, Abel L: A major susceptibility locus for HTLV-1 infection in childhood maps to chromosome 6q27. Hum Mol Genet. 2006, 15 (22): 3306-3312. 10.1093/hmg/ddl406.View ArticlePubMedGoogle Scholar
- Ureta-Vidal A, Angelin-Duclos C, Tortevoye P, Murphy E, Lepere JF, Buigues RP, Jolly N, Joubert M, Carles G, Pouliquen JF, et al: Mother-to-child transmission of human T-cell-leukemia/lymphoma virus type I: implication of high antiviral antibody titer and high proviral load in carrier mothers. Int J Cancer. 1999, 82 (6): 832-836. 10.1002/(SICI)1097-0215(19990909)82:6<832::AID-IJC11>3.0.CO;2-P.View ArticlePubMedGoogle Scholar
- Excoffier L, Laval G, Schneider S: Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evol Bioinform Online. 2005, 1: 47-50.PubMed CentralGoogle Scholar
- Coudray C, Olivieri A, Achilli A, Pala M, Melhaoui M, Cherkaoui M, El-Chennawi F, Kossmann M, Torroni A, Dugoujon JM: The complex and diversified mitochondrial gene pool of Berber populations. Ann Hum Genet. 2009, 73 (2): 196-214. 10.1111/j.1469-1809.2008.00493.x.View ArticlePubMedGoogle Scholar
- Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N: Reanalysis and revision of the Cambridge reference sequence for human mitochondrial DNA. Nat Genet. 1999, 23 (2): 147-10.1038/13779.View ArticlePubMedGoogle Scholar
- Brion M, Sobrino B, Blanco-Verea A, Lareu MV, Carracedo A: Hierarchical analysis of 30 Y-chromosome SNPs in European populations. Int J Legal Med. 2005, 119 (1): 10-15. 10.1007/s00414-004-0439-2.View ArticlePubMedGoogle Scholar
- Hammer MF, Spurdle AB, Karafet T, Bonner MR, Wood ET, Novelletto A, Malaspina P, Mitchell RJ, Horai S, Jenkins T, et al: The geographic distribution of human Y chromosome variation. Genetics. 1997, 145 (3): 787-805.PubMed CentralPubMedGoogle Scholar
- Kazanji M, Gessain A: Human T-cell Lymphotropic Virus types I and II (HTLV-I/II) in French Guiana: clinical and molecular epidemiology. Cad Saude Publica. 2003, 19 (5): 1227-1240. 10.1590/S0102-311X2003000500002.View ArticlePubMedGoogle Scholar
- Tortevoye P, Tuppin P, Peneau C, Carles G, Gessain A: Decrease of human T-cell lymphotropic virus type I prevalence and low incidence among pregnant women from a high endemic ethnic group in French Guiana. Int J Cancer. 2000, 87 (4): 534-538. 10.1002/1097-0215(20000815)87:4<534::AID-IJC12>3.0.CO;2-M.View ArticlePubMedGoogle Scholar
- Tuppin P, Lepere JF, Carles G, Ureta-Vidal A, Gerard Y, Peneau C, Tortevoye P, de The G, Moreau JP, Gessain A: Risk factors for maternal HTLV-I infection in French Guiana: high HTLV-I prevalence in the Noir Marron population. J Acquir Immune Defic Syndr Hum Retrovirol. 1995, 8 (4): 420-425.View ArticlePubMedGoogle Scholar
- Moynet D, Pouliquen JF, Londos-Gagliardi D, Buigues RP, Moreau JF, Bedjabaga I, Georges MC, Talarmin A, Joubert M, Fleury H, et al: High variability of HTLV-I in a remote population of Gabon as compared to that of a similar population of French Guiana. Virus Genes. 2001, 23 (3): 257-261. 10.1023/A:1012513121028.View ArticlePubMedGoogle Scholar
- Mahieux R, Horal P, Mauclere P, Mercereau-Puijalon O, Guillotte M, Meertens L, Murphy E, Gessain A: Human T-cell lymphotropic virus type 1 gag indeterminate western blot patterns in Central Africa: relationship to Plasmodium falciparum infection. J Clin Microbiol. 2000, 38 (11): 4049-4057.PubMed CentralPubMedGoogle Scholar
- Meertens L, Rigoulet J, Mauclere P, Van Beveren M, Chen GM, Diop O, Dubreuil G, Georges-Goubot MC, Berthier JL, Lewis J, et al: Molecular and phylogenetic analyses of 16 novel simian T cell leukemia virus type 1 from Africa: close relationship of STLV-1 from Allenopithecus nigroviridis to HTLV-1 subtype B strains. Virology. 2001, 287 (2): 275-285. 10.1006/viro.2001.1018.View ArticlePubMedGoogle Scholar
- Cassar O, Capuano C, Bassot S, Charavay F, Duprez R, Afonso PV, Abel M, Walter H, Mera W, Martin PM, et al: Human T lymphotropic virus type 1 subtype C melanesian genetic variants of the Vanuatu Archipelago and Solomon Islands share a common ancestor. J Infect Dis. 2007, 196 (4): 510-521. 10.1086/519167.View ArticlePubMedGoogle Scholar
- Cassar O, Capuano C, Meertens L, Chungue E, Gessain A: Human T-cell leukemia virus type 1 molecular variants, Vanuatu, Melanesia. Emerg Infect Dis. 2005, 11 (5): 706-710.PubMed CentralView ArticlePubMedGoogle Scholar
- Schlecht J, Kaplan ME, Barnard K, Karafet T, Hammer MF, Merchant NC: Machine-learning approaches for classifying haplogroup from Y chromosome STR data. PLoS Comput Biol. 2008, 4 (6): e1000093-10.1371/journal.pcbi.1000093.PubMed CentralView ArticlePubMedGoogle Scholar
- Salas A, Richards M, De la Fe T, Lareu MV, Sobrino B, Sanchez-Diz P, Macaulay V, Carracedo A: The making of the African mtDNA landscape. Am J Hum Genet. 2002, 71 (5): 1082-1111. 10.1086/344348.PubMed CentralView ArticlePubMedGoogle Scholar
- Salas A, Richards M, Lareu MV, Scozzari R, Coppa A, Torroni A, Macaulay V, Carracedo A: The African diaspora: mitochondrial DNA and the Atlantic slave trade. Am J Hum Genet. 2004, 74 (3): 454-465. 10.1086/382194.PubMed CentralView ArticlePubMedGoogle Scholar
- Forster P, Torroni A, Renfrew C, Rohl A: Phylogenetic star contraction applied to Asian and Papuan mtDNA evolution. Mol Biol Evol. 2001, 18 (10): 1864-1881.View ArticlePubMedGoogle Scholar
- Polzin TDSV: On Steiner trees and minimum spanning trees in hypergraphs. Operations Research Letters. 2003, 32: 12-20. 10.1016/S0167-6377(02)00185-2.View ArticleGoogle Scholar
- Tishkoff SA, Gonder MK, Henn BM, Mortensen H, Knight A, Gignoux C, Fernandopulle N, Lema G, Nyambo TB, Ramakrishnan U, et al: History of click-speaking populations of Africa inferred from mtDNA and Y chromosome genetic variation. Mol Biol Evol. 2007, 24 (10): 2180-2195. 10.1093/molbev/msm155.View ArticlePubMedGoogle Scholar
- Maca-Meyer N, Arnay M, Rando JC, Flores C, Gonzalez AM, Cabrera VM, Larruga JM: Ancient mtDNA analysis and the origin of the Guanches. Eur J Hum Genet. 2004, 12 (2): 155-162. 10.1038/sj.ejhg.5201075.View ArticlePubMedGoogle Scholar
- Dupanloup I, Bertorelle G: Inferring admixture proportions from molecular data: extension to any number of parental populations. Mol Biol Evol. 2001, 18 (4): 672-675.View ArticlePubMedGoogle Scholar
- Salas A, Carracedo A, Richards M, Macaulay V: Charting the ancestry of African Americans. Am J Hum Genet. 2005, 77 (4): 676-680. 10.1086/491675.PubMed CentralView ArticlePubMedGoogle Scholar
- Achilli A, Rengo C, Battaglia V, Pala M, Olivieri A, Fornarino S, Magri C, Scozzari R, Babudri N, Santachiara-Benerecetti AS, et al: Saami and Berbers--an unexpected mitochondrial DNA link. Am J Hum Genet. 2005, 76 (5): 883-886. 10.1086/430073.PubMed CentralView ArticlePubMedGoogle Scholar
- Allard MW, Polanskey D, Miller K, Wilson MR, Monson KL, Budowle B: Characterization of human control region sequences of the African American SWGDAM forensic mtDNA data set. Forensic Sci Int. 2005, 148 (2-3): 169-179. 10.1016/j.forsciint.2004.06.001.View ArticlePubMedGoogle Scholar
- Ely B, Wilson JL, Jackson F, Jackson BA: African-American mitochondrial DNAs often match mtDNAs found in multiple African ethnic groups. BMC Biol. 2006, 4: 34-10.1186/1741-7007-4-34.PubMed CentralView ArticlePubMedGoogle Scholar
- Melton T, Clifford S, Kayser M, Nasidze I, Batzer M, Stoneking M: Diversity and heterogeneity in mitochondrial DNA of North American populations. J Forensic Sci. 2001, 46 (1): 46-52.View ArticlePubMedGoogle Scholar
- Lind JM, Hutcheson-Dilks HB, Williams SM, Moore JH, Essex M, Ruiz-Pesini E, Wallace DC, Tishkoff SA, O'Brien SJ, Smith MW: Elevated male European and female African contributions to the genomes of African American individuals. Hum Genet. 2007, 120 (5): 713-722. 10.1007/s00439-006-0261-7.View ArticlePubMedGoogle Scholar
- Monsalve MV, Hagelberg E: Mitochondrial DNA polymorphisms in Carib people of Belize. Proc Biol Sci. 1997, 264 (1385): 1217-1224. 10.1098/rspb.1997.0168.PubMed CentralView ArticlePubMedGoogle Scholar
- Benn-Torres J, Bonilla C, Robbins CM, Waterman L, Moses TY, Hernandez W, Santos ER, Bennett F, Aiken W, Tullock T, et al: Admixture and population stratification in African Caribbean populations. Ann Hum Genet. 2008, 72 (Pt 1): 90-98.PubMedGoogle Scholar
- Hunemeier T, Carvalho C, Marrero AR, Salzano FM, Pena SDJ, Bortolini MC: Niger-Congo speaking populations and the formation of the Brazilian gene pool: mtDNA and Y-chromosome data. Am J Phys Anthropol. 2007, 133 (2): 854-867. 10.1002/ajpa.20604.View ArticlePubMedGoogle Scholar
- Silva WA, Bortolini MC, Schneider MP, Marrero A, Elion J, Krishnamoorthy R, Zago MA: MtDNA haplogroup analysis of black Brazilian and sub-Saharan populations: implications for the Atlantic slave trade. Hum Biol. 2006, 78 (1): 29-41. 10.1353/hub.2006.0028.View ArticlePubMedGoogle Scholar
- Batista dos Santos SE, Rodrigues JD, Ribeiro-dos-Santos AK, Zago MA: Differential contribution of indigenous men and women to the formation of an urban population in the Amazon region as revealed by mtDNA and Y-DNA. Am J Phys Anthropol. 1999, 109 (2): 175-180. 10.1002/(SICI)1096-8644(199906)109:2<175::AID-AJPA3>3.0.CO;2-#.View ArticlePubMedGoogle Scholar
- Ribeiro-dos-Santos AK, Carvalho BM, Feio-dos-Santos AC, dos Santos SE: Nucleotide variability of HV-I in Afro-descendents populations of the Brazilian Amazon Region. Forensic Sci Int. 2007, 167 (1): 77-80. 10.1016/j.forsciint.2005.12.033.View ArticlePubMedGoogle Scholar
- Carvalho BM, Bortolini MC, Batista dos Santos SE, Ribeiro-dos-Santos AK: Mitochondrial DNA mapping of social-biological interactions in Brazilian Amazonian African-descendant populations. Genetics and Molecular Biology. 2008, 31 (1): 12-22. 10.1590/S1415-47572008000100002.View ArticleGoogle Scholar
- Feio-Dos-Santos AC, Carvalho BM, Batista dosSantos SE, Ribeiro-dos-Santos AK: Nucleotide variability of HV-I in admixed population of the Brazilian Amazon Region. Forensic Sci Int. 2006, 164 (2-3): 276-277. 10.1016/j.forsciint.2005.12.032.View ArticlePubMedGoogle Scholar
- Rando JC, Pinto F, Gonzalez AM, Hernandez M, Larruga JM, Cabrera VM, Bandelt HJ: Mitochondrial DNA analysis of northwest African populations reveals genetic exchanges with European, near-eastern, and sub-Saharan populations. Ann Hum Genet. 1998, 62 (Pt 6): 531-550. 10.1046/j.1469-1809.1998.6260531.x.View ArticlePubMedGoogle Scholar
- Brehm A, Pereira L, Bandelt HJ, Prata MJ, Amorim A: Mitochondrial portrait of the Cabo Verde archipelago: the Senegambian outpost of Atlantic slave trade. Ann Hum Genet. 2002, 66 (Pt 1): 49-60. 10.1017/S0003480001001002.View ArticlePubMedGoogle Scholar
- Jackson BA, Wilson JL, Kirbah S, Sidney SS, Rosenberger J, Bassie L, Alie JA, McLean DC, Garvey WT, Ely B: Mitochondrial DNA genetic diversity among four ethnic groups in Sierra Leone. Am J Phys Anthropol. 2005, 128 (1): 156-163. 10.1002/ajpa.20040.View ArticlePubMedGoogle Scholar
- Rosa A, Brehm A, Kivisild T, Metspalu E, Villems R: MtDNA profile of West Africa Guineans: towards a better understanding of the Senegambia region. Ann Hum Genet. 2004, 68 (Pt 4): 340-352. 10.1046/j.1529-8817.2004.00100.x.View ArticlePubMedGoogle Scholar
- Watson E, Bauer K, Aman R, Weiss G, von Haeseler A, Paabo S: mtDNA sequence diversity in Africa. Am J Hum Genet. 1996, 59 (2): 437-444.PubMed CentralPubMedGoogle Scholar
- Rowold DJ, Luis JR, Terreros MC, Herrera RJ: Mitochondrial DNA geneflow indicates preferred usage of the Levant Corridor over the Horn of Africa passageway. J Hum Genet. 2007, 52 (5): 436-447. 10.1007/s10038-007-0132-7.View ArticlePubMedGoogle Scholar
- Coia V, Destro-Bisol G, Verginelli F, Battaggia C, Boschi I, Cruciani F, Spedini G, Comas D, Calafell F: Brief communication: mtDNA variation in North Cameroon: lack of Asian lineages and implications for back migration from Asia to sub-Saharan Africa. Am J Phys Anthropol. 2005, 128 (3): 678-681. 10.1002/ajpa.20138.View ArticlePubMedGoogle Scholar
- Cerny V, Salas A, Hajek M, Zaloudkova M, Brdicka R: A bidirectional corridor in the Sahel-Sudan belt and the distinctive features of the Chad Basin populations: a history revealed by the mitochondrial DNA genome. Ann Hum Genet. 2007, 71 (Pt 4): 433-452. 10.1111/j.1469-1809.2006.00339.x.View ArticlePubMedGoogle Scholar
- Destro-Bisol G, Coia V, Boschi I, Verginelli F, Caglia A, Pascali V, Spedini G, Calafell F: The analysis of variation of mtDNA hypervariable region 1 suggests that Eastern and Western Pygmies diverged before the Bantu expansion. Am Nat. 2004, 163 (2): 212-226. 10.1086/381405.View ArticlePubMedGoogle Scholar
- Batini C, Coia V, Battaggia C, Rocha J, Pilkington MM, Spedini G, Comas D, Destro-Bisol G, Calafell F: Phylogeography of the human mitochondrial L1c haplogroup: genetic signatures of the prehistory of Central Africa. Mol Phylogenet Evol. 2007, 43 (2): 635-644. 10.1016/j.ympev.2006.09.014.View ArticlePubMedGoogle Scholar
- Quintana-Murci L, Quach H, Harmant C, Luca F, Massonnet B, Patin E, Sica L, Mouguiama-Daouda P, Comas D, Tzur S, et al: Maternal traces of deep common ancestry and asymmetric gene flow between Pygmy hunter-gatherers and Bantu-speaking farmers. Proc Natl Acad Sci USA. 2008, 105 (5): 1596-1601. 10.1073/pnas.0711467105.PubMed CentralView ArticlePubMedGoogle Scholar
- Ingman M, Kaessmann H, Paabo S, Gyllensten U: Mitochondrial genome variation and the origin of modern humans. Nature. 2000, 408 (6813): 708-713. 10.1038/35047064.View ArticlePubMedGoogle Scholar
- Mateu E, Comas D, Calafell F, Perez-Lezaun A, Abade A, Bertranpetit J: A tale of two islands: population history and mitochondrial DNA sequence variation of Bioko and Sao Tome, Gulf of Guinea. Ann Hum Genet. 1997, 61 (Pt 6): 507-518.View ArticlePubMedGoogle Scholar
- Coelho M, Sequeira F, Luiselli D, Beleza S, Rocha J: On the edge of Bantu expansions: mtDNA, Y chromosome and lactase persistence genetic variation in southwestern Angola. BMC Evol Biol. 2009, 9 (80):
- Beleza S, Gusmao L, Amorim A, Carracedo A, Salas A: The genetic legacy of western Bantu migrations. Hum Genet. 2005, 117 (4): 366-375. 10.1007/s00439-005-1290-3.View ArticlePubMedGoogle Scholar
- Soodyall H, Vigilant L, Hill AV, Stoneking M, Jenkins T: mtDNA control-region sequence variation suggests multiple independent origins of an "Asian-specific" 9-bp deletion in sub-Saharan Africans. Am J Hum Genet. 1996, 58 (3): 595-608.PubMed CentralPubMedGoogle Scholar
- Pinto F, Gonzalez AM, Hernandez M, Larruga JM, Cabrera VM: Genetic relationship between the Canary Islanders and their African and Spanish ancestors inferred from mitochondrial DNA sequences. Ann Hum Genet. 1996, 60 (Pt 4): 321-330. 10.1111/j.1469-1809.1996.tb01195.x.View ArticlePubMedGoogle Scholar
- Vigilant L, Stoneking M, Harpending H, Hawkes K, Wilson AC: African populations and the evolution of human mitochondrial DNA. Science. 1991, 253 (5027): 1503-1507. 10.1126/science.1840702.View ArticlePubMedGoogle Scholar
- Krings M, Salem AE, Bauer K, Geisert H, Malek AK, Chaix L, Simon C, Welsby D, Di Rienzo A, Utermann G, et al: mtDNA analysis of Nile River Valley populations: A genetic corridor or a barrier to migration?. Am J Hum Genet. 1999, 64 (4): 1166-1176. 10.1086/302314.PubMed CentralView ArticlePubMedGoogle Scholar
- Castri L, Tofanelli S, Garagnani P, Bini C, Fosella X, Pelotti S, Paoli G, Pettener D, Luiselli D: mtDNA variability in two Bantu-speaking populations (Shona and Hutu) from Eastern Africa: implications for peopling and migration patterns in sub-Saharan Africa. Am J Phys Anthropol. 2009, 140 (2): 302-311. 10.1002/ajpa.21070.View ArticlePubMedGoogle Scholar
- Horai S, Hayasaka K: Intraspecific nucleotide sequence differences in the major noncoding region of human mitochondrial DNA. Am J Hum Genet. 1990, 46 (4): 828-842.PubMed CentralPubMedGoogle Scholar
- Caglia A, Tofanelli S, Coia V, Boschi I, Pescarmona M, Spedini G, Pascali V, Paoli G, Destro-Bisol G: A study of Y-chromosome microsatellite variation in sub-Saharan Africa: a comparison between F(ST) and R(ST) genetic distances. Hum Biol. 2003, 75 (3): 313-330. 10.1353/hub.2003.0041.View ArticlePubMedGoogle Scholar
- Rosa A, Ornelas C, Jobling MA, Brehm A, Villems R: Y-chromosomal diversity in the population of Guinea-Bissau: a multiethnic perspective. BMC Evol Biol. 2007, 7: 124-10.1186/1471-2148-7-124.PubMed CentralView ArticlePubMedGoogle Scholar
- Knight A, Underhill PA, Mortensen HM, Zhivotovsky LA, Lin AA, Henn BM, Louis D, Ruhlen M, Mountain JL: African Y chromosome and mtDNA divergence provides insight into the history of click languages. Curr Biol. 2003, 13 (6): 464-473. 10.1016/S0960-9822(03)00130-1.View ArticlePubMedGoogle Scholar
- Cruciani F, Santolamazza P, Shen P, Macaulay V, Moral P, Olckers A, Modiano D, Holmes S, Destro-Bisol G, Coia V, et al: A back migration from Asia to sub-Saharan Africa is supported by high-resolution analysis of human Y-chromosome haplotypes. Am J Hum Genet. 2002, 70 (5): 1197-1214. 10.1086/340257.PubMed CentralView ArticlePubMedGoogle Scholar
- Berniell-Lee G, Calafell F, Bosch E, Heyer E, Sica L, Mouguiama-Daouda P, van der Veen L, Hombert JM, Quintana-Murci L, Comas D: Genetic and demographic implications of the Bantu expansion: insights from human paternal lineages. Mol Biol Evol. 2009, 26 (7): 1581-1589. 10.1093/molbev/msp069.View ArticlePubMedGoogle Scholar
- Kayser M, Brauer S, Schadlich H, Prinz M, Batzer MA, Zimmerman PA, Boatin BA, Stoneking M: Y chromosome STR haplotypes and the genetic structure of U.S. populations of African, European, and Hispanic ancestry. Genome Res. 2003, 13 (4): 624-634. 10.1101/gr.463003.PubMed CentralView ArticlePubMedGoogle Scholar
- Abe-Sandes K, Silva WA, Zago MA: Heterogeneity of the Y chromosome in Afro-Brazilian populations. Hum Biol. 2004, 76 (1): 77-86. 10.1353/hub.2004.0014.View ArticlePubMedGoogle Scholar
- Ribeiro GG, De Lima RR, Wiezel CE, Ferreira LB, Sousa SM, Rocha DM, Canas Mdo C, Nardelli-Costa J, Klautau-Guimaraes Mde N, Simoes AL, et al: Afro-derived Brazilian populations: male genetic constitution estimated by Y-chromosomes STRs and AluYAP element polymorphisms. Am J Hum Biol. 2009, 21 (3): 354-356. 10.1002/ajhb.20875.View ArticlePubMedGoogle Scholar
- Ribeiro-dos-Santos AK, Pereira JM, Lobato MR, Carvalho BM, Guerreiro JF, Batista dosSantos SE: Dissimilarities in the process of formation of Curiau, a semi-isolated Afro-Brazilian population of the Amazon region. Am J Hum Biol. 2002, 14 (4): 440-447. 10.1002/ajhb.10059.View ArticlePubMedGoogle Scholar
- Wood ET, Stover DA, Ehret C, Destro-Bisol G, Spedini G, McLeod H, Louie L, Bamshad M, Strassmann BI, Soodyall H, et al: Contrasting patterns of Y chromosome and mtDNA variation in Africa: evidence for sex-biased demographic processes. Eur J Hum Genet. 2005, 13 (7): 867-876. 10.1038/sj.ejhg.5201408.View ArticlePubMedGoogle Scholar
- Luis JR, Rowold DJ, Regueiro M, Caeiro B, Cinnioglu C, Roseman C, Underhill PA, Cavalli-Sforza LL, Herrera RJ: The Levant versus the Horn of Africa: evidence for bidirectional corridors of human migrations. Am J Hum Genet. 2004, 74 (3): 532-544. 10.1086/382286.PubMed CentralView ArticlePubMedGoogle Scholar
- Hammer MF, Chamberlain VF, Kearney VF, Stover D, Zhang G, Karafet T, Walsh B, Redd AJ: Population structure of Y chromosome SNP haplogroups in the United States and forensic implications for constructing Y chromosome STR databases. Forensic Sci Int. 2006, 164 (1): 45-55. 10.1016/j.forsciint.2005.11.013.View ArticlePubMedGoogle Scholar
- Stefflova K, Dulik MC, Pai AA, Walker AH, Zeigler-Johnson CM, Gueye SM, Schurr TG, Rebbeck TR: Evaluation of group genetic ancestry of populations from Philadelphia and Dakar in the context of sex-biased admixture in the Americas. PLoS One. 2009, 4 (11): e7842-10.1371/journal.pone.0007842.PubMed CentralView ArticlePubMedGoogle Scholar
- Sans M: Admixture studies in Latin America: from the 20th to the 21st century. Hum Biol. 2000, 72 (1): 155-177.PubMedGoogle Scholar
- Salas A, Richards M, Lareu MV, Sobrino B, Silva S, Matamoros M, Macaulay V, Carracedo A: Shipwrecks and founder effects: divergent demographic histories reflected in Caribbean mtDNA. Am J Phys Anthropol. 2005, 128 (4): 855-860. 10.1002/ajpa.20117.View ArticlePubMedGoogle Scholar
- Coelho M, Coia C, Alves V, Luiselli D, Useli A, Hagemeijer T, Amorim A, Destro-Bisol G, Rocha J: Human Microevolution and the Atlantic Slave Trade: A Case Study from Sao Tome. Current Anthropology. 2008, 49 (1): 134-143. 10.1086/524762.View ArticleGoogle Scholar
- Trovoada MJ, Pereira L, Gusmao L, Abade A, Amorim A, Prata MJ: Pattern of mtDNA variation in three populations from Sao Tome e Principe. Ann Hum Genet. 2004, 68 (Pt 1): 40-54. 10.1046/j.1529-8817.2003.00070.x.View ArticlePubMedGoogle Scholar
- Cruz E: Historia do Para. Belem, Governo do Estado do Para. 1973, 148-Google Scholar
- Donoghue E: Black women/white men: the sexual exploitation of female slaves in the Danish West Indies. 2002, Trenton, NJ: Africa World PressGoogle Scholar
- Hamshere C: The British in the Caribbean. 1972, Cambridge, Mass: Harvard University PressGoogle Scholar
- Tishkoff SA, Reed FA, Friedlaender FR, Ehret C, Ranciaro A, Froment A, Hirbo JB, Awomoyi AA, Bodo JM, Doumbo O, et al: The genetic structure and history of Africans and African Americans. Science. 2009, 324 (5930): 1035-1044. 10.1126/science.1172257.PubMed CentralView ArticlePubMedGoogle Scholar
- Harich N, Costa MD, Fernandes V, Kandil M, Pereira JB, Silva NM, Pereira L: The trans-Saharan slave trade - clues from interpolation analyses and high-resolution characterization of mitochondrial DNA lineages. BMC Evol Biol. 2010, 10: 138-10.1186/1471-2148-10-138.PubMed CentralView ArticlePubMedGoogle Scholar
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