Complex evolutionary history of the Mexican stoneroller Campostoma ornatum Girard, 1856 (Actinopterygii: Cyprinidae)
© Domínguez-Domínguez et al; licensee BioMed Central Ltd. 2011
Received: 29 November 2010
Accepted: 4 June 2011
Published: 4 June 2011
Studies of the phylogeography of Mexican species are steadily revealing genetic patterns shared by different species, which will help to unravel the complex biogeographic history of the region. Campostoma ornatum is a freshwater fish endemic to montane and semiarid regions in northwest Mexico and southern Arizona. Its wide range of distribution and the previously observed morphological differentiation between populations in different watersheds make this species a useful model to investigate the biogeographic role of the Sierra Madre Occidental and to disentangle the actions of Pliocene tecto-volcanic processes vs Quaternary climatic change. Our phylogeographic study was based on DNA sequences from one mitochondrial gene (cytb, 1110 bp, n = 285) and two nuclear gene regions (S7 and RAG1, 1822 bp in total, n = 56 and 43, respectively) obtained from 18 to 29 localities, in addition to a morphological survey covering the entire distribution area. Such a dataset allowed us to assess whether any of the populations/lineages sampled deserve to be categorised as an evolutionarily significant unit.
We found two morphologically and genetically well-differentiated groups within C. ornatum. One is located in the northern river drainages (Yaqui, Mayo, Fuerte, Sonora, Casas Grandes, Santa Clara and Conchos) and another one is found in the southern drainages (Nazas, Aguanaval and Piaxtla). The split between these two lineages took place about 3.9 Mya (CI = 2.1-5.9). Within the northern lineage, there was strong and significant inter-basin genetic differentiation and also several secondary dispersal episodes whit gene homogenization between drainages. Interestingly, three divergent mitochondrial lineages were found in sympatry in two northern localities from the Yaqui river basin.
Our results indicate that there was isolation between the northern and southern phylogroups since the Pliocene, which was related to the formation of the ancient Nazas River paleosystem, where the southern group originated. Within groups, a complex reticulate biogeographic history for C. ornatum populations emerges, following the taxon pulse theory and mainly related with Pliocene tecto-volcanic processes. In the northern group, several events of vicariance promoted by river or drainage isolation episodes were found, but within both groups, the phylogeographic patterns suggest the occurrence of several events of river capture and fauna interchange. The Yaqui River supports the most diverse populations of C. ornatum, with several events of dispersal and isolation within the basin. Based on our genetic results, we defined three ESUs within C. ornatum as a first attempt to promote the conservation of the evolutionary processes determining the genetic diversity of this species. They will likely be revealed as a valuable tool for freshwater conservation policies in northwest Mexico, where many environmental problems concerning the use of water have rapidly arisen in recent decades.
The relative role of geology and ecology in driving lineage differentiation, and ultimately speciation, has been an important topic in evolutionary biology since the 19th century. At present, it is a thriving field of research aiming to disentangle the relative importance of glacial refugia, extrinsic environmental factors and species interactions, aided to the ability of locating species boundaries, phylogeographical breaks and hybrid zones .
Montane ecosystems are areas of high endemism, and the mechanisms driving this endemicity have been receiving increasing attention. For instance, endemism in tectonically active regions should reflect cladogenesis within the montane region, rather than the contraction of geographic range from a much larger region (, but see also ). Additionally, climatic factors have been rejected as the only explanation for the species richness of taxa within small to medium-sized geographic ranges; thus, both geological and evolutionary processes must be considered . Molecular studies of montane Mexican taxa often revealed complex phylogeographical patterns, and those high levels of genetic divergence suggest an underestimation of the level of endemism in the Mexican highlands, implying that more surveys in other co-distributed taxa are needed to achieve a better understanding of the evolutionary drivers of diversification in these regions .
What is clear at the present time is that the evolutionary history of many Meso- and North American taxa is linked to the severe geological (tectonic and volcanic) and environmental changes that occurred during the Neogene and Quaternary periods [3, 6, 7]. The challenge now is to distinguish the relative contribution of each factor to the observed phylogeographic pattern. The number of studies that have been conducted in the Mexican endemic-rich area of the northern sierras is lower than the number of studies of the Trans-Mexican Volcanic Belt (TMVB) (e.g., ). Therefore, one of the main contributions of the present work is to investigate the biogeographic role of the Sierra Madre Occidental (SMOC) using an endemic freshwater species. The SMOC may have acted as either a barrier where current admixture of lineages can occur, as a corridor for range expansion or as a centre of diversification, promoting allopatric differentiation.
Biogeographic role of the Sierra Madre Occidental
The SMOC is a large volcanic plateau in western Mexico that extends parallel to the Pacific coastline for more that 1200 km from the US-Mexico border (31° N) to the TMVB (21° N). The topography of the northern Sierra Madre is characterised by a high average elevation (1909 m). In contrast to other North American mountain systems, the topographic relief of the SMOC is not the product of elevated mountain ranges, but rather of incised canyons. The western edge is quite steep, while the eastern topographic gradients from the Sierra Madre into the central Mexican Plateau are relatively smooth .
The SMOC is thought to form an important corridor of dispersal for tropical flora and fauna moving in response to climatic change [10, 11]. This mountain system is one of the 14 biogeographic provinces of Mexico and one of the five biogeographic provinces of the so-called Mexican Transition Zone, mainly defined by the plant and animal taxa that are found above 1000 m. This province has the highest Nearctic influence .
The SMOC is the result of Cretaceous-Cenozoic magmatic and tectonic episodes related to the subduction of the Farallon plate beneath North America and to the opening of the Gulf of California. During the Oligocene, extensional tectonics (processes associated with the stretching of the lithosphere) began in the entire eastern half of the SMOC, migrating towards the west during the Miocene . The resulting post-Oligocene river capture has been inferred from geological data from the southern flank of the Tepic-Zacoalco rift (southern SMOC), although more recent piracy phenomena have been reported in other areas ( and references therein) or suggested by phylogeographic studies (e.g., ).
To the best of our knowledge, the phylogeography of ichthyofauna endemic to the SMOC has not been thoroughly investigated, although population genetics surveys have been conducted for Oncorhynchus chrysogaster ( and references therein) and Poeciliopsis sonorensis . As pointed out by recent literature , there are only a limited number of surveys on the genetic structure of endemic species from the North and Mesoamerican mountains (e.g., [17, 18]).
We consider two alternative biogeographic scenarios. Given the fact that the SMOC acts as a biogeographic barrier for many taxa, a plausible first hypothesis would be that the phylogeographic pattern of Campostoma ornatum would result from the current admixture of formerly West and East lowland lineages isolated during the Pleistocene interglacial stages [19–22]. If so, we would expect to find major lineages located on the northwestern and southeastern slopes and an altitudinal gradient of haplotype diversity. A second putative role for the SMOC would be as a centre of diversification, related to a working hypothesis of a highland distribution of C. ornatum predating the Quaternary ice ages. Under such a scenario, deep genetic and morphological divergences between the study species would have been shaped by geological processes rather than by climatic oscillations. Additionally, extensional tectonics causing river piracy (a geomorphological phenomenon occurring when a stream or river drainage system is diverted from its own bed and connects with the one of a neighbouring stream) would cause a mismatch between the actual conformation of river drainages and genetic patterns.
The widespread distribution of C. ornatum is thought have been shaped by tectonics (stream capture between adjacent drainages) and climatic changes (dispersion via periodically formed floodplains or the connection or succession of pluvial lakes during glacial stages) . Its distribution area (the elevation range of the species is 800 - 2000 m a.s.l.) coincides with a biodiversity hotspot, the the Madrean Pine-Oak Woodlands .
Several cyprinid species for which deep intraspecific genetic divergence has been previously reported exist in large areas severely influenced by both tecto-volcanic activity and climatic changes [26–28]. In fact, some of those widespread species have been split into more than one taxon, in light of their genetic divergence and morphological differentiation [14, 29, 30]. This was the case for Campostoma anomalum, which was previously regarded as a complex of three widespread and morphologically divergent subspecies, whose five distinct evolutionary lineages warranted species status [31, 32]. The species Campostoma ornatum, also with a wide distribution range and morphological variation, is also likely to show deep intraspecific genetic differentiation because populations from southern drainages (Nazas, Aguanaval and Piaxtla rivers) show a differentiated morphotype  and there is remarkable endemicity in the ichthyofauna of the Nazas river [33, 34].
Phylogeography also has an applied perspective from the conservation point of view. The genetic and morphological information obtained in the present work will be examined to define, if appropriate, evolutionarily significant units (ESUs) within Campostoma ornatum. The need to preserve the loss of Campostoma lineages restricted to areas where aquatic biodiversity is imperilled was already highlighted . In addition, the populations of the Mexican stoneroller in the USA declined during the 20th century  and there were plans to perform reintroductions from Mexico .
At the present time, an ESU is essentially acknowledged to be a population or a group of populations that merit the separate management or priority of conservation because it is highly distinctive, according to the following criteria: (i) reproductive isolation and adaptation, (ii) reciprocal monophyly and (iii) "exchangeability" of populations. Concordance across multiple data types (e.g., genetic, morphological and geographic) is, of course, desirable (revised by ). Defining conservation units in endemic species may be of particular interest in densely populated countries, such as Mexico. This is because environmental problems concerning the use of water rapidly arise in semiarid regions, such as northwest Mexico [38, 39], where the demand for water resources has dramatically increased in recent decades [40, 41].
In the present study we aim to infer the evolutionary history of C. ornatum throughout its distribution area in order to determine the role of the SMOC on the evolutionary history of C. ornatum and to disentangle the action of tecto-volcanic processes vs. climatic change. To do this, we applied phylogenetic and population genetic methods to sequence data obtained for a mitochondrial gene (cytochrome b, cytb) and two nuclear fragments (RAG1 and S7) from 18 to 29 locations within the distribution area of C. ornatum. In addition, we included several meristic characters to first corroborate the morphological variation pattern previously observed  and second to investigate the putative association between genetic and phenotypic data. In line with our main objective, we also addressed the following question from the conservation perspective: do any of the populations/lineages sampled in different drainages deserve to be categorised as an (ESU) under the Adaptive Evolutionary Conservation (AEC) framework ?
Sampled localities and material analysed.
Locality and elevation
(metres above sea level)
Genbank accession number
San Juan River in the Cuarto area, San Juan del Río town, Durango, Mex. (1525)
JF343058 (8), JF343059 (2)
Ramos River, El Olote town, Durango, Mex. (1652)
JF343039 (2), JF343041 (4), JF343042 (1), JF343070 (1), JF343071 (1), JF343072 (1), JF343073 (1)
3.Peñón Blanco (PBY)
Stream outskirts of Peñón Blanco Town, Durango, Mex. (1739)
JF343057 (10), JF343078 (1)
Covadonga River, at Peñon Blanco Town, Durango, Mex. (1730)
Ramos River, Atotonilco town, Durango, Mex. (1693)
JF343039 (1), JF343040 (1), JF343041 (7), JF343042 (1)
Piaxtla River, La Quinta town, Durango, Mex. (2391)
JF343110 (1), JF343113 (2)
7.Sain Alto (SAA)
Sain Alto River near Atotonilco, Zacatecas, Mex. (1999)
8.Río Primero (RIP)
Primero River, road Torreón de Mata town, Chihuahua, Mex. (1720)
JF343105 (1), JF343106 (1)
JF343116 (1), JF343120 (1)
River at San Francisco Javier de Satevo town, Chihuahua, Mex. (1450)
JF343084 (2), JF343085 (1)
Villa Ocampo, Chihuahua, Mex. (1832)
JF343055 (2), JF343066 (1)
El Porvenir River, road La Magdalena town-Balleza town, Chihuahua, Mex. (1579)
JF343055 (1), JF343056 (1), JF343079 (8)
Florido River at Villa Coronado town, Chihuahua, Mex. (1511)
JF343055 (10), JF343056 (1)
Conchos River at Bocoyna town, Mex. Chihuahua, Mex. (2219)
Urique River road Guachochi town-Creel town, Chihuahua, Mex. (1647)
Cuzarare stream, at Rimichurachi town, S of Creel, Chihuahua, Mex. (2196)
JF343081 (2), JF343082 (5), JF343083 (3)
Oteros River Southwest to Creel town, Chihuahua, Mex. (2065)
CPUM7169, CPUM7172, CPUM7175-CPUM7182, CPUM7229-CPUM7230
JF343074 (11), JF343075 (1)
Aguacaliente River at Basaseachic town, Chihuahua, Mex. (2005)
JF343043 (9), JF343044 (1)
Concheño River, road El Placer town-Ocampo town, Chihuaua, Mex. (2225)
River at La Tauna town, Chihuahua, Mex. (1764)
JF343043 (4), JF343086(5), JF343087 (1)
Tomochic River at Tomochic town, Chihuahua, Mex. (2210)
JF343076 (4), JF343092 (1), JF343093 (1), JF343094 (1), JF343095 (1)
Río Papigochic at ranch near to Pahuirachic, Chihuahua, Mex. (1764)
JF343076 (11), JF343077 (1)
South of Moctezuma, Sonora, Mex. (558)
JF343088 (3), JF343089 (6), JF343090 (1), JF343091 (1)
Bavispe River, Huachinera town, Sonora, Mex. (1062)
CPUM7669-CPUM7670, CPUM7674-CPUM7676, CPUM7678-CPUM7682
Bavispe River at Los Hondables, Morelos, Sonora, Mex. (857)
JF343064 (9), JF343065 (1)
25.Agua Prieta (PRI)
Stream road Agua Prieta city-Janos city, Sonora, Mex. (1113)
JF343047 (4), JF343048 (8), JF343080 (5)
JF343117 (7), JF343118 (4)
Cabullona River, road Agua Prieta city-Nacozari de García city, Sonora, Mex. (1134)
JF343046 (5), JF343047 (3), JF343048 (6), JF343049 (1)
JF343099 (1), JF343100 (13)
JF343117 (10), JF343118 (1)
27.Ojo de Agua (OJO)
Spring at road Cananea city-Bacoachi town, Sonora, Mex. (1386)
JF343067 (3), JF343068 (2), JF343069 (5)
28.Casas Grandes (GRA)
Casas Grandes River at Hacienda San Diego, S of Casas Grandes city, Chihuahua, Mex. (1510)
JF343060 (1), JF343061 (6), JF343062 (1), JF343063 (2)
29.I. Zaragoza (ZAR)
Casas Grandes River at Ignacio Zaragoza town, Chihuahua, Mex. (2077)
JF343060 (4), JF343096 (1), JF343097 (4), JF343098 (1)
30.Santa Clara (CLA)
Santa Clara River at San Lorenzo, East of Buenaventura, Chihuahua, Mex. (1524)
JF343050 (4), JF343051 (1), JF343052 (1), JF343053 (4), JF343054 (1)
Measurements of genetic diversity for Campostoma ornatum as revealed by 1110 bp of the mitochondrial cytb and calculated according to sampling location.
0.533 ± 0.180
0.0020 ± 0.0012
0.2 ± 0.154
0.0002 ± 0.0001
0.733 ± 0.067
0.0174 ± 0.0021
Santa Clara (CLA)
0.8 ± 0.1
0.0019 ± 0.0004
Villa Coronado (COR)
0.182 ± 0.144
0.0007 ± 0.0005
El Cuarto (CUA)
0.356 ± 0.159
0.0003 ± 0.0001
Casas Grandes (GRA)
0.644 ± 0.152
0.0012 ± 0.0003
Los Hondables (HON)
0.2 ± 0.154
0.0002 ± 0.0001
Villa Ocampo (OCA)
0.667 ± 0.314
0.003 ± 0.0014
Ojo de Agua (OJO)
0.689 ± 0.104
0.0011 ± 0.0003
El Olote (OLO)
0.873 ± 0.089
0.0044 ± 0.0011
0.167 ± 0.134
0.0001 ± 0.0001
0.167 ± 0.134
0.0003 ± 0.0002
Peñón Blanco-Yerbaniz (PBY)
0.182 ± 0.144
0.0002 ± 0.0001
0.378 ± 0.181
0.0007 ± 0.0004
Agua Prieta (PRI)
0.676 ± 0.064
0.0166 ± 0.0023
La Quinta (QNT)
0.689 ± 0.104
0.0009 ± 0.0001
0.667 ± 0.314
0.0006 ± 0.0003
La Tauna (TAU)
0.644 ± 0.101
0.0008 ± 0.0003
0.673 ± 0.123
0.0018 ± 0.0007
0.786 ± 0.151
0.0009 ± 0.0002
Ignacio Zaragoza (ZAR)
0.733 ± 0.101
0.0017 ± 0.0004
Genetic diversity measurements for the eight cytb phylogroups obtained by the 95% SP unconnected subnetworks for Campostoma ornatum.
FS (95% C.I.)
-0.77 (-3.66, 3.88)
0.11 (0.06, 0.23)
0.95 (-1.96, 3.34)
0.21 (0.14, 0.3)
0.08 (-3.73, 4.28)
0.14 (0.07, 0.2)
-0.50 (-3.2, 3.59)
0.14 (0.11, 0.26)
1.09 (-0.34, 1.09)
0.28 (0.18, 0.3)
4.16 (-9.36, 7.18)
0.12 (0.05, 0.16)
-2.51 (-5.99, 5.48)
0.07 (0.05, 0.18)
1.55 (-6.76, 5.68)
0.14 (0.05, 0.17)
We sequenced the S7 nuclear gene in 56 specimens from 19 locations, representing all surveyed river basins. Twenty-eight of these individuals had between one and seven heterozygous positions. After haplotype reconstruction, 24 variants were defined by 36 variable sites (plus four indels) along the 843 bp of the final alignment (37 mutations in total). Two of the variable sites were singletons, and 34 substitutions were parsimony informative. Overall, haplotype diversity (h) was 0.862 ± 0.02, nucleotide diversity (π) = 0.008 ± 0.001, the average number of nucleotide differences (k) = 6.99 and Tajima's D = -0.066 (not significant). Fourteen sites showed insertion/deletion variation. Indel polymorphism was estimated in four indel events and haplotypes (average indel length event = 3.5, indel haplotype diversity = 0.169). When heterozygous positions are coded as unknown but gaps are kept as the fifth state, the number of haplotypes decreases to 15, defined by 18 variable sites (plus indels). Haplotype diversity (h) was reduced to 0.632 ± 0.068, nucleotide diversity (π) = 0.0047 ± 0.0008, the average number of nucleotide differences (k) = 3.84 and Tajima's D = -0.0602 (not significant). Three of the variable sites correspond to singletons, and 15 substitutions were parsimony informative.
The populations from Santa Clara (Santa Clara River basin) and El Olote (Nazas River basin) were the most diverse, as measured by the number of haplotypes, gene diversity and allelic richness (Table 2). Seven populations, scattered along the Fuerte, Mayo, Nazas, Yaqui and Conchos basins, contained only one haplotype (Table 2). Divergent haplotypes were found in Cabullona (CAB) and Agua Prieta (PRI), both in the Yaqui River basin, as shown by the number of segregating sites (S) and average number of nucleotide differences (k) (Table 2). The possibility of admixture of lineages led us to increase the number of sequenced individuals at these two sites to 15 and 17, respectively. When genetic diversity parameters were calculated according to the phylogroups indicated by the 95% SP unconnected subnetworks (see below), phylogroups VI (Yaqui, San Bernardino and Mayo River locations) and VIII (Nazas, Aguanaval and Piaxtla River locations) were the most diverse, whereas phylogroup V (Cabullona and San Bernardino River locations) was the least diverse (Table 3).
Twenty-two of the 43 individuals for which the RAG1 nuclear gene was sequenced had between one and three heterozygotic positions. After haplotype reconstruction, 12 variants were defined by 12 variable sites along the 979 bp of the final alignment (total number of mutations = 12). No gaps were found. Overall, haplotype diversity (h) was 0.847 ± 0.021, nucleotide diversity (π) = 0.00228 ± 0.00014, the average number of nucleotide differences (k) = 2.23 and Tajima's D = -0.166 (not significant). One variable site was a singleton and 11 substitutions were parsimony informative. Eight substitutions corresponded to synonymous changes, with the other four implicating amino acid replacements. If heterozygous positions were coded as unknown, six haplotypes were obtained for the 44 individuals mentioned above. These haplotypes were defined by six variable sites (total number of mutations = 6). Two of these sites were singletons, and the remaining four substitutions were parsimony informative. Two changes involved amino acid replacement, and four were synonymous. Overall, haplotype diversity (h) was 0.568 ± 0.077 (standard deviation), nucleotide diversity (π) = 0.00087 ± 0.00018, the average number of nucleotide differences (k) = 0.8425 and Tajima's D = -1.017 (not significant).
Within the northern lineage, several incongruities were present among the three loci. The nuclear loci showed a large basal polytomy for all groups, as expected for fewer variable markers. The better resolved mitochondrial topology (Figure 2), largely congruent with all of the analyses conducted (NJ, BI and ML), showed a large polytomy forming three groups: one included populations within the Yaqui and Sonora drainages; a second group comprised populations within the Conchos, Fuerte and Santa Clara drainages; and a third group was formed by populations within Yaqui and Casas Grandes drainages. This pattern did not recover the geographic pattern of the drainages, as only the Santa Clara River drainage conformed to a monophyletic group. All of the other drainages showed mixed haplotypes (Figure 2), and the southern population showed mixed haplotypes between drainages.
Most of the subnetworks were composed of haplotypes found in more than one drainage; only Santa Clara (CLA) (subnetwork IV) and Ojo de Agua (OJO) (within the Sonora basin; subnetwork II) contained private haplotypes not shared with any other subnetwork or basin (Figure 3). Although subnetworks III and V were composed of haplotypes found in only one basin (Conchos and Yaqui), haplotypes from Conchos also appeared in subnetwork I, whereas haplotypes from Yaqui were also present in subnetworks VI and VII (Figure 3). We found that haplotypes from the Tomochic and Papigochic sites (subnetwork VI) grouped in a star-like shape, as did haplotypes from the Casas Grandes (GRA) and Ignacio Zaragoza (ZAR) populations (within subnetwork VII).
Several divergent lineages were found in two particular locations: some haplotypes from Cabullona (CAB) and Agua Prieta (PRI) (both in the Yaqui River basin) formed subnetwork V, whereas other haplotypes from CAB and PRI were connected to different river basins (Mayo from subnetwork VI, Casas Grandes from subnetwork VII). This points to a distinct evolutionary history for each of those mtDNA lineages currently sympatric within the Yaqui River basin.
The estimated age of the most recent common ancestor (TMRCA) of the northern and southern lineages was 3.9 Mya (2.1-5.9). The diversification events within the main northern lineage were dated back to the Lower Pleistocene (Figure 2).
Morphological character values registered in the present study are consistent with the variation pattern obtained by prior literature  throughout the range of C. ornatum, particularly the counts in lateral line (LLS), predorsal (PrDS) and the circumferential scales (CircS). The most significant result is the distinction between the southern (mitochondrial phylogroup VIII) and northern (I-VII phylogroups) lineages, as revealed by 54-71 LLS (usually 65-66) vs 62-81 (usually 69-72), 25-32 PrDS (usually 27-29) vs 29-40 (usually 31-34) and 44-55 CircS (usually 48) vs 50-61 (usually 52-55) (Figure 1 and Additional file 1). Despite the high inter-drainage and inter-population variation recorded, this morphological pattern supports the deep genetic divergence between the Nazas-Aguanaval-Piaxtla basins and the northern drainages (Figures 2, 3, 4, and 5). In contrast, morphological variation within northern group was not congruent with the genetic phylogenies inferred. For instance, specimens from Santa Clara basin (lineage IV) are clearly differentiated from the other northern drainages, regardless of the surveyed genetic marker (Figures 2, 3, 4, and 5). However, their morphological variation appeared overlapped with regard to other lineages within the northern group, e.g., the three surveyed variables fall within the range found for Conchos drainage (Additional file 1).
Analysis of molecular variance (AMOVA) across a range of putative population groupings.
Variation accounted for (Φ)
Among populations within groups (ΦSC)
Within populations (ΦST)
All in one group
Phylogenetic groups (SP subnetworks)
[I] [II] [III] [IV] [V] [VI] [VII] [VIII]
[Nazas] [Piaxtla] [Conchos] [Fuerte] [Mayo]
[Yaqui] [Sonora] [Casas Grandes] [Santa Clara]
SAMOVA K = 4
([Piaxtla+Nazas] [Conchos+Santa Clara+Fuerte]
[Yaqui1(Cab, Pap, Pri, Tau, Ter, Tom)+Mayo+Sonora]
[Casas Grandes + Yaqui2(Hon, Hua)]
SAMOVA K = 13
([Casas Grandes+Yaqui2(Hon, Hua)] [Nazas1(Cov, Pby)]
[Mayo+Yaqui1(Tau)] [Nazas2(Ato, Olo)] [Piaxtla] [Conchos1(Por, Sat)]
[Conchos2(Cor, Oca, Rip)] [Santa Clara] [Nazas3(Cua)]
[Yaqui3(Pap, Ter, Tom)] [Yaqui4(Cab, Pri)] [Conchos3(Bcy)+Fuerte]
[Conchos][all other localities]
Therefore, we analysed the demographic history of mtDNA lineages (i.e., phylogroups in 95% SP unconnected subnetworks), rather than sampling sites or SAMOVA groupings. This way, we could infer which lineages had experienced expansions, instead of trying to infer demographic expansions in the contemporary populations containing haplotypes of mixed ancestry. Neutrality tests applied to each phylogroup revealed no deviations from neutrality due to population expansion. The only lineage with Fs and R2 statistics close to significance was the one composed of subnetwork V (containing some of the individuals from CAB and PRI, Yaqui River basin) (Table 3). The mismatch distribution analysis for this phylogroup was consistent with the sudden expansion model (SDD = 0.04, p = 0.16; raggedness index = 0.32, p = 0.12; τ = 0.869, 95% CI = 0-1.818) and showed a unimodal distribution that closely fit the expected distribution (data not shown).
Our genetic and morphological results unambiguously showed two well-differentiated evolutionary groups within C. ornatum, supporting prior preliminary investigations . The first lineage is located in the northern river drainages (Yaqui, Mayo, Fuerte, Sonora, Casas Grandes, Santa Clara and Conchos), whereas the other lineage occurs in the southernmost drainages of the species' area of distribution (Nazas, Aguanaval and Piaxtla; central SMOC). The large number of mutation steps between the two lineages exceeded the 95% parsimony limits for the cytb and S7 genes, a result indicative of a long history of isolation between these clades. Such an allopatric fragmentation dated back to the Pliocene (3.9 Mya, CI = 2.1-5.9), as revealed by the Bayesian coalescent analyses. The absence of a sharp differentiation between the western (Pacific drainages) and eastern (Atlantic or endorheic basins) lineages, together with the lack of an altitudinal gradient of haplotype diversity (data not shown) lead us to discard a recent (Upper Quaternary) origin for the observed phylogeographic pattern.
In the light of this, and following the proposal of distinguishing between competing models of diversification for North and Mesoamerica , we can emphasise tecto-volcanic processes as the crucial factors influencing the evolutionary history of C. ornatum, rather than recent habitat fluctuations caused, for instance, by alternating glacial/interglacial stages. The genetic pattern we observed allowed us to infer common river piracy phenomena.
The most plausible biogeographic scenario for the separation of northern and southern groups (mean divergences P = 5.2%) is an early Pliocene vicariant event that split a widespread common ancestor. According to our dating, the allopatric fragmentation between the northern and southern lineages took place around 3.9 Mya (CI = 2.2-5.9). This scenario is also supported by the high genetic diversity found in phylogroup V (Naza-Aguanaval-Piaxtla) (Table 3), which is congruent with a high number of female founders followed by long term population stability. Observations from the southern lineage specimens examined in the present study (Nazas and Piaxtla) are in agreement with prior findings , as they showed a differentiated morphotype distinguished by higher counts in the lateral, predorsal and circumferential scales, as well as a deeper body. Such a genetic and morphologic differentiation warrants a taxonomic revision likely involving the description of a different taxonomic entity (Figure 2).
The existence of an ancient paleosystem related to the Nazas River has been put forward in prior literature in light of the distribution of its ichthyofauna and herpetofauna [44–46]. Numerous fish species are endemic to the Nazas River , which may be indicative of a independent evolutionary history of those species in isolation. Recent molecular surveys also support the existence of an ancient isolation of the Conchos River system and the Nazas, Aguanaval, Mezquital Rivers, a vicariant event that has been dated to ca. 5 Mya based on the divergence between the two groups of the genus Cyprinodon (Actinopterygii, Ciprinodontiformes) . The ancient connection and disruption of the Nazas palaeosystem with the Conchos River is well supported by the distribution of conspecific species in the Gila, Cyprinella, Ictiobus and Cyprinodon genera [23, 48–50]. Thus, to the best of our knowledge, the presence of C. ornatum in the Aguanaval and Piaxtla Rivers is caused by a recent dispersal event from Nazas River, as revealed by the phylogenetic trees, the cytb network and ΦST results. This hypothesis is also in agreement with the biogeographic scenario postulated for semi-aquatic snakes of the genus Thamnophis .
Northern lineage genetic structure and demography
A complex, reticulate biogeographic history in time and space emerged within the northern C. ornatum populations, following a clear pattern of taxon pulse episodes . This model assumes that species and their adaptations arise in "centres of diversification" and that distributional ranges of taxa periodically fluctuate around a more stable, continuously occupied centre. This general biotic dispersal may be interrupted by the formation of barriers, producing episodes of vicariant speciation. Breakdown of those barriers produces new episodes of biotic expansion, setting the stage for yet more episodes of vicariance . At this point, it is worth noting the dispersal ability of our model species. We assumed the Mexican stoneroller to be a sedentary species. Its limited active dispersal may putatively be enhanced by environmental factors, such as predation or abiotic change, as has been described for other congeneric species [53, 54].
Fuerte and Conchos
Although the Fuerte and Conchos River populations form a well-differentiated group (P = 2.3% ± 0.4% between Conchos and Fuerte Rivers populations, excluding the BCY and URI populations), pointing to a long evolutionary history in isolation (ca. 1.7 Mya, CI = 0.8-2.9, Figure 2), a recent dispersal event from the Fuerte to Conchos River systems is evident. We base this argument on four lines of evidence: (i) the Urique population (URI, Fuerte basin) shares a haplotype with the Bocoyna population (BCY, Conchos River) (Figure 3), (ii) both locationns have non-significant ΦST values, (iii) the position of the URI population in the phylogenetic tree (Figure 2) and (iv) the concordance of the pattern in mitochondrial subnetwork I with the hypothesis of recent (post-glacial) divergence . The close proximity between the headwaters of Conchos and Fuerte River basins makes highly likely the occurrence of a stream capture event between them, as suggested by . Other species co-distributed between these basins, such as Codoma ornata, Gila pulchra and Catostomus plebeius , may have experienced the same dispersal event.
Guzman endorheic basin
All markers revealed that the Santa Clara population is the most divergent (mean divergence p = 2.9%). This result disagrees with the existence of the hypothesised ancient pluvial Lake Palomas [56, 57], which would have covered much of the Guzman Basin systems and would have become fragmented into a series of isolated, endorheic rivers, lakes and springs during the Upper Quaternary (ca. 200 000 years ago). This event would have formed the current Guzman basin, which include the Mimbres, Casas Grandes, Santa Maria and Santa Clara River endorheic basins . Our molecular dating showed more ancient episodes of isolation for both the Santa Clara (ca. 2.1 Mya (CI = 1-3.7)) and the Casas Grandes (isolated about 1.5 Mya (CI = 0.4-2.8)) populations (Figure 2). Moreover, the latter was more closely related to samples from the Bavispe River sub-basin (Yaqui River Drainage) than to samples from Santa Clara.
The close relationship between the fish fauna from the Yaqui tributaries and the Casas Grandes system can be attributed to an episode of dispersal from the Casas Grandes to the Yaqui River. This hypothesis is supported by the position of the Yaqui River samples (HON, CAV, HUA and PRI) in the phylogenetic tree (Figure 2) and network (Figure 3), as well as by their low genetic diversity. Such a connection/dispersal event between Yaqui and Casas Grandes is also supported by (i) the occurrence of other co-distributed species (such as Cyprinella formosa ) and (ii) the finding of very closely related species in each of those river basins (e.g., Cyprinodon pisteri and Cyprinodon albivelis, formerly considered the same species [59, 60], occur at Casas Grandes and Yaqui, respectively ).
Thus far, the Yaqui River supports the most diverse C. ornatum populations. Haplotypes found in this northern basin are found in three different mitochondrial subnetworks (including subnetwork VII, as previously explained) (Figure 3) and their supported clades (Figure 2), which was confirmed by the nuclear dataset. Subnetworks V and VI are mainly composed by haplotypes occurring at the Yaqui tributaries and diverged from the other clades around 3 Mya (CI = 1.5-4.9) (Figure 2). This indicates an initial and ancient vicariant event between the Yaqui River and its contiguous basins (Mayo, Sonora and Casas Grandes), followed by episodes of dispersal and subsequent isolation.
Concerning descriptors of genetic diversity, the mitochondrial subnetwork VI had the greatest number of haplotypes, segregating sites, haplotype diversity and average number of differences (Table 3). In light of this, we suggest a dispersal event from the Yaqui to the Mayo River, an event supported by the position of the Mayo populations (BAS and CON) in the phylogenetic tree. Moreover, the La Tauna population (TAU, within Yaqui River) shares a haplotype with the Concheño (CON) and Basaseachic (BAS) populations, both from the Mayo River, and also shows a non-significant pairwise ΦST value, which may be indicative of recent gene flow. Faunal exchanges between the Yaqui River and surrounding areas have been previously reported [15, 62]. In fact, it has been argued that dispersal between the Yaqui and its contiguous basins was, putatively, a reason for the high fish biodiversity in the Yaqui River .
The Yaqui River system hosts three divergent haplogroups (subnetworks V, VI and VII) that occur in sympatry at the Cabullona and Agua Prieta locations (both belonging to Agua Prieta stream, an upper tributary of the Bavispe sub-basin). One of those haplogroups clusters with haplotypes found in the Casas Grandes River forming subnetwork VII. Haplotype cab7845 falls within the second haplogroup (subnetwork VI), closely related with haplotypes form the Tomochic and Papigochic sites (Yaqui River). The third lineage (subnetwork V) is composed by only two haplotypes and shows a mean genetic divergence of P = 1.5%, also suggesting a relatively long isolation. Interestingly, the Terapa population (TER), also belonging to the Yaqui River basin, shows a high amount of divergence with respect to other Yaqui populations (P = 1.3%). These results are, again, congruent with episodes of isolation in different tributaries of the Yaqui River, followed by a secondary dispersal from some isolated demes to other Yaqui tributaries. We postulate this hypothesis as the most likely explanation for the presence of those three divergent mitochondrial lineages at Cabullona and Agua Prieta.
Haplotypes from the Ojo de Agua (OJO) site, a small spring located at the Sonora River Basin, belong to a separated subnetwork (II) and show large genetic distances (P = 2.1% ± 0.3%) with other members of its monophyletic group (Figure 2). This may be indicative of relatively long period of isolation, starting around 1.2 Mya (CI = 0.4-2.2). In addition, the low values of genetic diversity reported for these samples could be caused by a reduction in effective population size, as is expected due to the small size of the sampled area.
Implications for conservation and taxonomy
In the light of our genetic and morphologic results, we hereby define three ESUs (sensu ) within C. ornatum corresponding to the following groups: (i) the southern lineage, (ii) the northern lineage and (iii) the Yaqui river basin. While acknowledging that the Yaqui belongs to the northern lineage, we define this third sub-hierarchical ESU due to the existence of three deeply divergent lineages at Agua Prieta. Here, the term sub-hierarchical does not infer subordination but rather a frame of reference on a phylogenetic continuum . At present, only two of the sampled localities are currently protected by Mexican legislation (Urique and Huachinera), although the BAS sampling site is close to the Basaseachic Falls National Park. Therefore, the definition of these three ESUs shall promote the conservation of the evolutionary processes shaping the genetic pattern of C. ornatum throughout its range.
We found populations of C. ornatum with a complex, reticulate biogeographic history with repeated events of isolation and dispersal, which is consistent with the taxon pulse theory. This complex history could be related to the dynamic tecto-volcanic activity that has occurred in the region since the early Pliocene. Both genetics and morphology revealed a strong differentiation between the southern and northern populations of C. ornatum. In light of this, a taxonomic revision is necessary to clarify the taxonomic status of the two well-differentiated groups within Campostoma ornatum. The differentiation between the northern and southern taxa was likely due to the isolation of the paleosystem that connected the Nazas with other northern river basins during the Pliocene. In addition, we determined the presence of three very divergent mitochondrial lineages occurring concurrently in the northern Agua Prieta Stream (Yaqui river basin). We postulated that episodes of isolation and the subsequent admixture of lineages from different sources could explain the co-occurrence of these lineages at Yaqui. In addition, three ESUs are proposed that cover the full range of the Mexican stoneroller. The pattern obtained for Campostoma ornatum paves the way for a future comparative phylogeographical study aimed at disentangling the evolutionary factors that shape the genetic structure of this species and its populations in the northwest of Mexico.
Specimen collection and laboratory procedures
A total of 285 Camposoma ornatum individuals were collected at 29 locations in northwest Mexico (Figure 1). Between 3 and 17 specimens were analysed per location (Table 1). Organisms were caught using a seine net and electrofishing. A small sample of tissue from the caudal fin from each fish was cut and preserved in 96% ethanol and stored in the laboratory at -20°C prior to analysis. Voucher specimens were fixed in 10% formalin and preserved in 70% ethanol and deposited in the Colección de Peces de la Universidad Michoacana (CPUM) (Table 1). To confirm the taxonomic identity of C. ornatum, morphological examination was performed following , i.e., using the three most informative meristic characters: (i) number of lateral line scales, (ii) number of predorsal scales and (iii) number of circumferential scales. We analysed ten specimens corresponding to, at least, one population from each major drainage, except for Aguanaval and Mayo (Additional file 1).
Genomic DNA was extracted from a <0.25 cm2 piece of the pectoral fin using the High Pure™ PCR Template Preparation Kit (Roche Diagnostics GmbH, Mannheim, Germany), following the manufacturer's instructions. We amplified a 1140-bp fragment of the mitochondrial cytochrome b gene (cytb) in 285 individuals by Polymerase Chain Reaction (PCR) and the GLUf - THRr primer pair .
Amplifications were performed in 30-μL reaction volumes containing 1× PCR buffer (5PRIME), 1 U Taq DNA polymerase (5PRIME), 0.2 mM of each dNTP, 0.2 μM of each primer and 50-100 ng of DNA. Similar conditions were employed to amplify the first intron of the S7 ribosomal protein gene in 56 specimens (1000 bp, primers S7RPEX1F and S7RPEX2R ) and approximately 1400 bp of the recombinant activation gene 1 (RAG1) in 43 samples (primers RAG1F1 and RAG1R1 ).
PCR for cytb started with an initial denaturing step at 95°C for 2 minutes, followed by 35 amplification cycles at 94°C for 1 minute, 56°C for 1.5 minutes, 65°C for 1 minute and a final extension step at 65°C for 7 minutes. PCR for S7 started with an initial denaturing step at 95°C for 2 minutes, followed by 30 amplification cycles at 95°C for 1 minute, 59.5°C for 1.5 minutes, 65°C for 2 minutes and a final extension step at 65°C for 7 minutes. PCR for RAG1 started with an initial denaturing step at 94°C for 2 minutes, followed by 40 amplification cycles at 94°C for 45 seconds, 56-58°C for 1 minute, 68°C for 2 minutes and a final extension step at 68°C for 7 minutes.
PCR products were electrophoresed on 2% agarose gels and visualised under UV light after ethidium bromide staining. Cytb PCR products were purified and bidirectionally sequenced at the DNA Sequencing Service (Macrogen, Seoul, Korea). S7 and RAG1 PCR products were purified and bidirectionally sequenced on a 3130 × l Genetic Analyser (Applied Biosystems, ABI) at SAI (Sequencing Facility, University of A Coruña, Spain). New sequences were deposited in GenBank (Table 1).
Alignment and diversity analyses
DNA electropherograms were checked and aligned using CODONCODES 3.5.6 (CodonCode Corporation, Dedham, Massachusetts). Mitochondrial alignments were straightforward. Apart from the 285 individuals sequenced for the present work, phylogenetic analyses included three Campostoma ornatum cytb sequences available in public databases for (accession numbers EU082476, DQ324062 and FJ913814). For phylogenetic analyses, RAG1 alignments included sequence EU082549 from Durango (Nazas River basin). Mitochondrial alignments were straightforward and unambiguous. For the analysis of nuclear DNA, the sites where individuals contained heterozygous genotypes for the sampled nuclear loci and any heterozygous base pair positions were coded using standard degeneracy codes . Heterozygous sites in S7 and RAG1 sequences were resolved to two haplotypes per individual using the PHASE feature of DNAsp v.5.1 , which uses the algorithms from PHASE 2.1.1 . We employed the recombination model (MR0), setting the output probability for both genotypes and haplotypes to 0.95. Following , runs consisted of 500 iterations as burn-in, 500 main iterations and a thinning interval = 1.
Haplotypes and their frequencies, standard indices of genetic variation such as the number of segregating sites (S), nucleotide diversity per gene (π), haplotype diversity (h) and the average number of nucleotide differences (k) were calculated using DNAsp 5.1 . Allelic richness (R) was computed after rarefaction to three individuals  using CONTRIB (available at http://www.pierroton.inra.fr/genetics/labo/Software/) for all populations that included at least three individuals.
As DNA variation may arise through different molecular evolution models, we ran jMODELTEST 0.1.1  to select the one that best matched our three datasets following Akaike's criterion: for the cytb gene, we obtained the General Time Reversible model with a gamma distribution shaped with α = 0.175, F81 for S7 and K80+I for RAG1. These models were used to estimate the phylogenetic relationships between all populations of C. ornatum based on the mitochondrial cytb gene and the S7 and RAG1 nuclear genes. Phylogenetic trees were calculated using the neighbour-joining (NJ), Bayesian inference (BI) and maximum likelihood (ML) methods. Distance analysis was carried out as implemented in PAUP *4.0b10  for 1000 bootstrap replicates. BI was performed in MrBayes , and two independent analyses of four MCMC were run with 9,000,000 and 5,000,000 generations for mitochondrial and nuclear loci, respectively, sampling every 100 generations. Once the convergence and stationarity were verified by a suitable effective sample size (ESS) for all parameters in Tracer v.1.4.1 , "burn in" consisting in removing the first 10% of generations was performed and posterior probabilities were obtained from a majority-rule consensus. The Maximum Likelihood (ML) phylogeny was inferred using PhyML 3.0  using the subtree pruning and regrafting (SPR) tree searching option. Branch support was assessed after 1000 bootstrap replicates as well as with the Shimodaira-Hasegawa-like (SH) procedure implemented in PhyML. We used Campostoma oligolepis (accession number DQ324064) as an outgroup to root the tree. Using sequences from Campostoma pullum (EU082477) or Campostoma pauciradii (DQ324065) as outgroup did not significantly change any of the results shown in the present study.
The phylogeny of the haplotypes was also inferred using the statistical parsimony (SP) criterion . The 95% SP network was calculated using TCS 1.2 . As several unconnected networks were obtained, we then forced the TCS algorithm to connect all subnetworks with a fixed connection limit of 50 steps. To have a reference to calibrate the extent of divergence between haplogroups, we rooted the resulting network with a homologous sequence from Campostoma pauciradii (DQ324065) by forcing a connection limit of 130 steps. Similarly, 95% SP networks were calculated for S7 and RAG1 datasets, both using only homozygous positions (i.e., heterozygous coded as unknown) and the separated haplotypes inferred by PHASE.
We estimated divergence times among sets of mitochondrial sequences and their associated credibility intervals using the Bayesian coalescent approach as implemented in BEAST 1.4.6 . Markov chain Monte Carlo (MCMC) simulations were run with the following specifications: the GTR model of evolution, uncorrelated lognormal distribution, the Yule tree process as a prior and a branch length substitution rate sampled from a prior normal distribution (mean value = 0.010, standard deviation = 0.001). All simulations were run for 50 million generations, sampling chains every 1000 generations. Because of the lack of an appropriate fossil record for the genus Campostoma, we applied a rate of molecular evolution of 1.05% per million years, calibrated for the cytb in North American Phoxinini  and widely applied to cyprinids [14, 80]. The results of three independent runs (with an UPGMA starting tree) were loaded and combined in TRACER 1.5  to check for convergence on a stationary distribution, determine burn-in and assess effective sample size (ESS) and frequency plots of the relevant parameters. Ten percent of the trees were discarded as burn-in, and the remaining ones were combined in the BEAST module LogCombiner 1.4.6. Lastly, the BEAST module TreeAnnotator 1.4.6 was used to calculate the timescale.
We tested for evidence of population subdivision under different criteria using an analysis of molecular variance (AMOVA) as implemented in ARLEQUIN v.188.8.131.52 . We also used ARLEQUIN to test for genetic differentiation between pairs of populations using FST analogues (ΦST) based on the Tamura-Nei model of sequence evolution. Statistical significance of covariance components (and Φ-statistics) from AMOVAs and pairwise ΦST values was determined on the basis of the distribution of values obtained from 1000 permutations of the data.
We also assessed population structuring using the simulated annealing procedure as implemented in SAMOVA (Spatial Analysis of Molecular Variance) 1.0 . We used 100 simulated annealing processes for k values from 2 to 29.
The demographic history of the cytb phylogroups was first explored using Fu's Fs  and R2  statistics. Their significance was assessed through the 95% confidence interval after 1000 coalescent simulations that were conditional on the number of segregating sites, as implemented in DNAsp.
We characterised the demographic expansions detected for all phylogroups, rejecting the null hypothesis under the Fs and R2 tests with the mismatch distribution of the pairwise genetic differences , as implemented in ARLEQUIN. Goodness-of-fit to a sudden expansion model was tested using parametric bootstrap approaches (1000 replicates). The sum of squared deviations (SSD) between the observed and expected mismatch distributions was used to assess the significance of the test.
We thank Rosa García and Lourdes Alcaraz for their valuable help in the lab. We appreciate the comments and corrections suggested by two anonymous referees and Prof. Miguel Vences. This investigation was financially supported by grants from Chester Zoo Garden, PROMEP PTC-232 for ODD and the University of A Coruña to MV. RPR is grateful to Consejo Nacional de Ciencia y Tecnología (CONACyT) for a PhD scholarship (No.195290).
- Rissler LJ, Smith WH: Mapping amphibian contact zones and phylogeographical break hotspots across the United States. Mol Ecol. 2010, 19: 5404-5416. 10.1111/j.1365-294X.2010.04879.x.View ArticlePubMed
- Badgley C: Tectonics, topography, and mammalian diversity. Ecography. 2010, 33: 220-231.
- Ledig FT, Hodgskiss PD, Jacob-Cervantes V: Genetic diversity, mating system, and conservation of a Mexican subalpine relict, Picea mexicana Martinez. Conserv Genet. 2002, 3: 113-122. 10.1023/A:1015297621884.View Article
- Voelker G, Outlaw RK, Bowier RCK: Pliocene forest dynamics as a primary driver of African bird speciationge. Global Ecol Biogeogr. 2010, 19: 111-121. 10.1111/j.1466-8238.2009.00500.x.View Article
- Bryson RW, Murphy RW, Lathrop A, Lazcano-Villareal D: Evolutionary drivers of phylogeographical diversity in the highlands of Mexico: a case study of the Crotalus triseriatus species group of montane rattlesnakes. J Biogeogr. 2011, 38: 697-710. 10.1111/j.1365-2699.2010.02431.x.View Article
- Zarza E, Reynoso VH, Emerson BC: Diversification in the northern neotropics: mitochondrial and nuclear DNA phylogeography of the iguana Ctenosaura pectinata and related species. Mol Ecol. 2008, 17: 3259-3275. 10.1111/j.1365-294X.2008.03826.x.View ArticlePubMed
- Moreno-Letelier A, Piñero D: Phylogeographic structure of Pinus strobiformis Engelm. across the Chihuahuan Desert filter-barrier. J Biogeogr. 2009, 36: 121-131. 10.1111/j.1365-2699.2008.02001.x.View Article
- Demastes JW, Spradling TA, Hafner MS, Hafner DJ, Reed DL: Systematics and phylogeography of pocket gophers in the genera Cratogeomys and Pappogeomys. Mol Phylogenet Evol. 2002, 22: 144-154. 10.1006/mpev.2001.1044.View ArticlePubMed
- Coblentz DD, Riitters KH: Topographic controls on the regional-scale biodiversity of the south-western USA. J Biogeogr. 2004, 31: 1125-1138. 10.1111/j.1365-2699.2004.00981.x.View Article
- Ceballos G, Arroyo-Cabrales J, Ponce E: Effects of Pleistocene environmental changes on the distribution and community structure of the mammalian fauna of Mexico. Quaternary Res. 2010, 73: 464-473. 10.1016/j.yqres.2010.02.006.View Article
- Morrone JJ: Fundamental biogeographic patterns across the Mexican Transition Zone: an evolutionary approach. Ecography. 2010, 33: 355-361.
- Ferrari L, Valencia-Moreno M, Bryan S: Magmatismo y tectónica en la Sierra Madre Occidental y su relación con la evolución de la margen occidental de Norteamérica. Bol Soc Geol Mex. 2005, LVII: 343-378.
- Montgomery DR, Lopez-Blanco J: Post-Oligocene river incision, southern Sierra Madre Occidental, Mexico. Geomorphology. 2003, 55: 235-247. 10.1016/S0169-555X(03)00142-9.View Article
- Pérez-Rodríguez R, Domínguez-Domínguez O, Pérez-Ponce de León G, Doadrio I: Phylogenetic relationships and biogeography of the genus Algansea Girard 1856 (Cypriniformes:Cyprinidae) from central Mexico inferred from molecular data. BMC Evol Biol. 2009, 9: 223-10.1186/1471-2148-9-223.View ArticlePubMedPubMed Central
- Camarena-Rosales F, Ruiz-Campos G, De La Rosa-Vélez J, Mayden RL, Hendrickson DA, Varela-Romero A, García de León FJ: Mitochondrial haplotype variation in wild trout populations (Teleostei: Salmonidae) from northwestern Mexico. Rev Fish Biol Fisher. 2008, 18: 33-45. 10.1007/s11160-007-9060-z.View Article
- Hedrick PW, Lee RN, Hurt CR: The endangered Sonoran topminnow: Examination of species and ESUs using three mtDNA genes. Conserv Genet. 2006, 7: 483-492. 10.1007/s10592-005-9058-9.View Article
- Goldberg CS, Sullivan BK, Malone JH, Schwalbe CR: Divergence among barking frogs (Eleutherodactylus augusti) in the southwestern United States. Herpetologica. 2004, 60: 312-320. 10.1655/03-81.View Article
- Anducho-Reyes MA, Cognato AI, Hayes JL, Zúñiga G: Phylogeography of the bark beetle Dendroctonus mexicanus Hopkins (Coleoptera: Curculionidae: Scolytinae). Mol Phylogenet Evol. 2008, 49: 930-940. 10.1016/j.ympev.2008.09.005.View ArticlePubMed
- Metcalfe SE, O'Hara SL, Caballero M, Davies SJ: Records of Late Pleistocene-Holocene climatic change in Mexico - a review. Quaternary Sci Rev. 2000, 19: 699-721. 10.1016/S0277-3791(99)00022-0.View Article
- Ortega-Rosas CI, Guiot J, Penalba MC, Ortiz-Acosta ME: Biomization and quantitative climate reconstruction techniques in northwestern Mexico - With an application to four Holocene pollen sequences. Global Planet Change. 2008, 61: 242-266. 10.1016/j.gloplacha.2007.10.006.View Article
- Mead JI, White RS, Baez A, Hollenshead MG, Swift SL, Carpenter MC: Late Pleistocene (Rancholabrean) Cynomys (Rodentia, Sciuridae: prairie dog) from northwestern Sonora, Mexico. Quat Int. 2010, 217: 138-142. 10.1016/j.quaint.2009.10.011.View Article
- Nunez EE, Macfadden BJ, Mead JI, Baez A: Ancient forests and grasslands in the desert: Diet and habitat of Late Pleistocene mammals from Northcentral Sonora, Mexico. Palaeogeogr Palaeoclimatol Palaeoecol. 2010, 297: 391-400. 10.1016/j.palaeo.2010.08.021.View Article
- Miller RR, Minkley WL, Norris SM: Freshwater fishes of Mexico. 2005, Chicago: University of Chicago Press, 1
- Burr BM: A review of the Mexican Stoneroller, Campostoma ornatum Girard (Pisces: Cyprinidae). Trans San Diego Soc Nat Hist. 1976, 18: 127-144.View Article
- Mittermeier RA, Robles Gil P, Hoffmann M, Pilgrim J, Brooks T, Goettsch Mittermeier C, Lamoreux J, Da Fonseca GAB: Hotspots Revisited: Earth's: Biologically Richest and Most Endangered Terrestrial Ecoregions. 2005, Chicago: Conservation International
- Gleeson DM, Howitt RLJ, Ling N: Genetic variation, population structure and cryptic species within the black mudfish, Neochanna diversus, an endemic galaxiid from New Zealand. Mol Ecol. 1999, 8: 47-57. 10.1046/j.1365-294X.1999.00528.x.View ArticlePubMed
- Colborn J, Crabtree RE, Shaklee JB, Pfeiler E, Bowen BW: The Evolutionary Enigma of Bonefishes (Albula spp.): Cryptic Species and Ancient Separations in a Globally Distributed Shorefish. Evolution. 2001, 55: 807-820. 10.1554/0014-3820(2001)055[0807:TEEOBA]2.0.CO;2.View ArticlePubMed
- Huey JA, Baker AM, Hughes JM: High levels of genetic structure in the Australian freshwater fish, Ambassis macleayi. J North Am Benthol Soc. 2010, 29: 1148-1160. 10.1899/09-093.1.View Article
- Pompa-Domínguez A, Doadrio I: A new species of the Genus Yuriria Jordan & Evermann, 1896 (Actinopterygii, Cyprinidae) from the Ameca Basin if the Central Mexican Plateau. Graellsia. 2007, 63: 259-271. 10.3989/graellsia.2007.v63.i2.93.View Article
- Domínguez-Domínguez O, Pérez-Rodríguez R, Escalera-Vázquez LH, Doadrio I: Two new species of the genus Notropis Rafinesque, 1817 (Actinopterygii, Cyprinidae) from the Lerma River Basin in Central Mexico. Hidrobiológica. 2009, 19: 159-172.
- Blum MJ, Neely DA, Harris PM, Mayden RL: Molecular Systematics of the Cyprinid Genus Campostoma (Actinopterygii: Cypriniformes): Disassociation between Morphological and Mitochondrial Differentiation. Copeia. 2008, 2008: 360-369. 10.1643/CI-06-093.View Article
- Cashner RC, Matthews WJ, Marsh-Matthews E, Unmack PJ, Cashner FM: Recognition and Redescription of a Distinctive Stoneroller from the Southern Interior Highlands. Copeia. 2010, 2010: 300-311. 10.1643/CI-08-051.View Article
- Contreras-Balderas S, Lozano-Vilano MD, García-Ramírez ME: Historical changes in the index of biological integrity for the lower Rio Nazas, Durango, Mexico. Historical changes in large river fish assemblages of the Americas. Edited by: Rinne JN, Hughes RM, Calamusso B. 2005, Bethesda, Maryland: American Fisheries Society Symposium, 45: 225-237.
- Domínguez-Domínguez O, Pérez-Ponce de León G: Is the Mesa Central of Mexico a biogeographical province? Descriptive analysis based on freshwater biotic components. Rev Mex Biodivers. 2009, 85: 835-852.
- Hendrickson DA, Minckley WL, Miller RR, Siebert DJ, Minckley PH: Fishes of the Río Yaqui basin, México and United States. J Arizona-Nevada Acad Sci. 1980, 15: 65-106.
- Arizona Game and Fish Department: Campostoma ornatum. Distributed by the Heritage Data Management System, Arizona Game and Fish Department. Phoenix, AZ. 2003, [http://www.gf.state.az.us/pdfs/w_c/hdms/Fish/Camporna.fo.pdf]
- Allendorf FW, Luikart G: Units of Conservation. Conservation and the Genetics of Populations. 2007, Oxford: Blackwell Publishing, 380-420.
- Lyons J, Navarro-Pérez S, Cochran PA, Santanta CE, Guzmán-Arroyo M: Index of Biotic Integrity Based on Fish Assemblages for the Conservation of Streams and Rivers in West-Central Mexico. Conserv Biol. 1995, 9: 569-584. 10.1046/j.1523-1739.1995.09030569.x.View Article
- Alcocer J, Bernal-Brooks FW: Limnology in Mexico. Hydrobiologia. 2010, 644: 15-68. 10.1007/s10750-010-0211-1.View Article
- Edwards RJ, Garrett GP, Marsh-Matthews E: Conservation and status of the fish communities inhabiting the Rıo Conchos basin and middle Rio Grande, Mexico and U.S.A. Rev Fish Biol Fisher. 2002, 12: 119-132. 10.1023/A:1025098229262.View Article
- Contreras-Balderas S, Almada-Villela P, Lozano-Vilano ML, García-Ramírez ME: Freshwater fish at risk or extinct in Mexico A checklist and review. Rev Fish Biol Fisher. 2003, 12: 241-251.View Article
- Fraser DJ, Bernatchez L: Adaptive evolutionary conservation: towards a unified concept for defining conservation units. Mol Ecol. 2001, 10: 2741-2752.View ArticlePubMed
- McCormack JE, Peterson AT, Bonaccorso E, Smith TB: Speciation in the highlands of Mexico: genetic and phenotypic divergence in the Mexican jay (Aphelocoma ultramarina). Mol Ecol. 2008, 17: 2505-2521. 10.1111/j.1365-294X.2008.03776.x.View ArticlePubMed
- Meek SE: The freshwater fishes of Mexico north of the isthmus of Tehuantepec. Field Columbian Mus Publ Zool Ser. 1904, 5: 1-252.
- Arellano RV: Research on the continental Neogene of Mexico. Am J Sci. 1951, 249: 604-616. 10.2475/ajs.249.8.604.View Article
- Conant R: Semiaquatic snakes of the genus Thamnophis from the isolated drainage system of the Río Nazas and adjacent areas in Mexico. Copeia. 1963, 1963: 473-499. 10.2307/1441468.View Article
- Echelle AA, Carson EW, Echelle AF, Bussche RAVD, Dowling TE, Meyer A: Historical Biogeography of the New-World Pupfish Genus Cyprinodon (Teleostei:Cyprinodontidae). Copeia. 2005, 2005: 220-239.View Article
- Contreras-Balderas S, Lozano ML: Cyprinella alvarezdelvillari, a New Cyprinid Fish from Río Nazas of México, with a Key to the Lepida Clade. Copeia. 1994, 1994: 897-906. 10.2307/1446712.View Article
- Echelle AA, Echelle AF: Evolutionary relationships of pupfishes in the Cyprinodon eximius complex (Atherinomorpha: Cyprinodontiformes). Copeia. 1998, 1998: 852-865. 10.2307/1447332.View Article
- Bart HL, Clementsb MD, Blantonb RE, Pillerb KR, Hurleyc DL: Discordant molecular and morphological evolution in buffalofishes (Actinopterygii: Catostomidae). Mol Phylogenet Evol. 2010, 56: 808-820. 10.1016/j.ympev.2010.04.029.View ArticlePubMed
- Erwin TL: Taxon pulses, vicariance, and dispersal: an evolutionary synthesis illustrated by carabid beetles. Vicariance Biogeography - a Critique. Edited by: Nelson G, Rosen DE. 1981, New York Columbia University Press, 159-196.
- Brooks DR: Historical biogeography in the age of complexity: Expansion and integration. Rev Mex Biodivers. 2005, 76: 79-94.
- Mundahl ND, Ingersoll CG: Home range, movements, and density of the central stoneroller, Campostoma anomalum, in a small Ohio stream. Environl Biol Fish. 1989, 24: 307-311. 10.1007/BF00001405.View Article
- Schaefer J: Rifflles as barriers to interpool movement by three cyprinids (Notropis boops, Campostoma anomalum and Cyprinella venusta). Freshwater Biol. 2001, 46: 379-388. 10.1046/j.1365-2427.2001.00685.x.View Article
- McCormack JE, Bowen BS, Smith TB: Integrating paleoecology and genetics of bird populations in two sky island archipelagos. BMC Biol. 2008, 6: 28-10.1186/1741-7007-6-28.View ArticlePubMedPubMed Central
- Reeves CC: Pluvial Lake Palomas, Northwestern Chihuahua, Mexico. Guidebook of the border region. Edited by: Córdoba DA, Wengard SA, Shomaker J. 1969, New Mexico Geological Society, 20th Annual Field Conference Guidebook, 143-154.
- Chernoff B, Miller RR: Notropis bocagrande, anew cyprinid fish from Chihuahua, Mexico, with comments on Notropis formosus. Copeia. 1982, 1982: 514-522. 10.2307/1444650.View Article
- Wood RM, Mayden RL: Speciation and anagenesis in the genus Cyprinella of Mexico (Teleostei: Cyprinidae): a case study of Model III allopatric speciation. Rev Fish Biol Fisher. 2002, 12: 253-271. 10.1023/A:1025018118339.View Article
- Echelle AA, Dowling TE: Mitochondrial DNA variation and evolution of the Death Valley pupfishes (Cyprinodon, Cyprinodontidae). Evolution. 1992, 46: 193-206. 10.2307/2409814.View Article
- Echelle AA, Echelle AE: An Allozyme perspective on mtDNA variation and evolution of the Death Valley pupfishes (Cyprinodon, Cyprinodontidae). Copeia. 1993, 1993: 275-287. 10.2307/1447128.View Article
- Minckley WL, Miller RR, Norris SM, Schaefer SA: Three New Pupfish Species, Cyprinodon (Teleostei, Cyprinodontidae), from Chihuahua, México, and Arizona, USA. Copeia. 2002, 2002: 687-705. 10.1643/0045-8511(2002)002[0687:TNPSCT]2.0.CO;2.View Article
- Minckley WL, Hendrickson DA, Bond CE: Geography of western North American freshwater fishes: description and relationships to intracontinental tectonism. Zoogeography of North American Freshwater fishes. Edited by: Hocutt CH, Wiley EO. 1986, New York: Wiley-Interscience Publications, 519-613.
- Zardoya R, Doadrio I: Phylogenetic relationships of Iberian cyprinids: systematic and biogeographical implications. Proc R Soc B-Biol Sci. 1998, 265: 1365-1372. 10.1098/rspb.1998.0443.View Article
- Chow S, Hazama K: Universal PCR primers for S7 ribosomal protein gene introns in fish. Mol Ecol. 1998, 7: 1247-1263.View Article
- López JA, Chen WJ, Ortí G: Esociform phylogeny. Copeia. 2004, 2004: 449-464. 10.1643/CG-03-087R1.View Article
- Bossu CM, Near TJ: Gene Trees Reveal Repeated Instances of Mitochondrial DNA Introgression in Orangethroat Darters (Percidae Etheostoma). Syst Biol. 2009, 58: 114-129. 10.1093/sysbio/syp014.View ArticlePubMed
- Librado P, Rozas J: DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009, 25: 1451-1452. 10.1093/bioinformatics/btp187.View ArticlePubMed
- Stephens M, Donnelly P: A comparison of Bayesian methods for haplotype reconstruction from population genotype data. Am J Hum Genet. 2003, 73: 1162-1169. 10.1086/379378.View ArticlePubMedPubMed Central
- Garrick RC, Sunnucks P, Dyer RJ: Nuclear gene phylogeography using PHASE: dealing with unresolved genotypes, lost alleles, and systematic bias in parameter estimation. BMC Evol Biol. 2010, 10: 118-10.1186/1471-2148-10-118.View ArticlePubMedPubMed Central
- Petit RJ, Mousadik AE, Pons O: Identifying Populations for Conservation on the Basis of Genetic Markers. Conserv Biol. 1998, 12: 844-855. 10.1046/j.1523-1739.1998.96489.x.View Article
- Posada D: jModelTest: Phylogenetic model averaging. Mol Biol Evol. 2008, 25: 1253-1256. 10.1093/molbev/msn083.View ArticlePubMed
- Swofford DL: Phylogenetic analysis using parsimony (* and other methods) V 4.0b 10. 2004, Sunderland MA: Sinauer Associates,
- Huelsenbeck JP, Ronquist F: MRBAYES: Bayesian inference of phylogeny. Bioinformatics. 2001, 17: 754-755. 10.1093/bioinformatics/17.8.754.View ArticlePubMed
- Rambaut A, Drummond AJ: Tracer v1.4. [http://beast.bio.ed.ac.uk/Tracer]
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syste Biol. 2003, 52: 696-704. 10.1080/10635150390235520.View Article
- Templeton AR, Crandall KA, Sing CF: A cladistic analysis of phenotypic association with haplotypes inferred from restriction endonuclease mapping and DNA sequence data. III. Cladogram estimation. Genetics. 1992, 132: 619-633.PubMedPubMed Central
- Clement M, Posada D, Crandall KA: TCS: a computer program to estimate gene genealogies. Mol Ecol. 2000, 9: 1657-1660. 10.1046/j.1365-294x.2000.01020.x.View ArticlePubMed
- Drummond AJ, Rambaut A: BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol. 2007, 7: 214-10.1186/1471-2148-7-214.View ArticlePubMedPubMed Central
- Dowling TE, Tibbets CA, Minckley WL, Smith GR: Evolutionary relatioships of the plagopterins (Teleostei:Cyprinidae) from cytochrome b sequences. Copeia. 2002, 2002: 665-678. 10.1643/0045-8511(2002)002[0665:EROTPT]2.0.CO;2.View Article
- Durand JD, Bianco PG, Laroche J, Gilles A: Insight into the origin of endemic Mediterranean ichthyofauna: Phylogeography of Chondrostoma genus (Teleostei, Cyprinidae). J Hered. 2003, 94: 315-328. 10.1093/jhered/esg074.View ArticlePubMed
- Excoffier L, Lischer HEL: Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour. 2010, 10: 564-567. 10.1111/j.1755-0998.2010.02847.x.View ArticlePubMed
- Dupanloup I, Schneider S, Excoffier L: A simulated annealing approach to define the genetic structure of populations. Mol Ecol. 2002, 11: 2571-2581. 10.1046/j.1365-294X.2002.01650.x.View ArticlePubMed
- Fu YX: Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics. 1997, 147: 915-925.PubMedPubMed Central
- Ramos-Onsins SE, Rozas J: Statistical properties of new neutrality tests against population growth. Mol Biol Evol. 2002, 19: 2092-2100.View ArticlePubMed
- Rogers AR, Harpending H: Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol. 1992, 9: 552-569.PubMed
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