Phylogeny and evolution of life-history strategies in the Sycophaginae non-pollinating fig wasps (Hymenoptera, Chalcidoidea)
© Cruaud et al; licensee BioMed Central Ltd. 2011
Received: 16 February 2011
Accepted: 22 June 2011
Published: 22 June 2011
Non-pollinating Sycophaginae (Hymenoptera, Chalcidoidea) form small communities within Urostigma and Sycomorus fig trees. The species show differences in galling habits and exhibit apterous, winged or dimorphic males. The large gall inducers oviposit early in syconium development and lay few eggs; the small gall inducers lay more eggs soon after pollination; the ostiolar gall-inducers enter the syconium to oviposit and the cleptoparasites oviposit in galls induced by other fig wasps. The systematics of the group remains unclear and only one phylogeny based on limited sampling has been published to date. Here we present an expanded phylogeny for sycophagine fig wasps including about 1.5 times the number of described species. We sequenced mitochondrial and nuclear markers (4.2 kb) on 73 species and 145 individuals and conducted maximum likelihood and Bayesian phylogenetic analyses. We then used this phylogeny to reconstruct the evolution of Sycophaginae life-history strategies and test if the presence of winged males and small brood size may be correlated.
The resulting trees are well resolved and strongly supported. With the exception of Apocrytophagus, which is paraphyletic with respect to Sycophaga, all genera are monophyletic. The Sycophaginae are divided into three clades: (i) Eukoebelea; (ii) Pseudidarnes, Anidarnes and Conidarnes and (iii) Apocryptophagus, Sycophaga and Idarnes. The ancestral states for galling habits and male morphology remain ambiguous and our reconstructions show that the two traits are evolutionary labile.
The three main clades could be considered as tribes and we list some morphological characters that define them. The same biologies re-evolved several times independently, which make Sycophaginae an interesting model to test predictions on what factors will canalize the evolution of a particular biology. The ostiolar gall-inducers are the only monophyletic group. In 15 Myr, they evolved several morphological adaptations to enter the syconia that make them strongly divergent from their sister taxa. Sycophaginae appears to be another example where sexual selection on male mating opportunities favored winged males in species with small broods and wingless males in species with large broods. However, some species are exceptional in that they lay few eggs but exhibit apterous males, which we hypothesize could be due to other selective pressures selecting against the re-appearance of winged morphs.
In many animal species, males that compete for females may adopt alternative reproductive tactics. These tactics translate in many cases into discontinuous variation in male morphology, behaviour or life history . Male dimorphism or polymorphism is common in species in which sexual selection is strong [2, 3] and takes place in a variety of taxonomic groups such as insects (e.g. bees and wasps [4, 5], damselflies , earwigs , dung-beetles ); other invertebrates (e.g. spiders , opiliones , mites , amphipods  and nematodes ) and vertebrates [14, 15].
Among these groups, fig wasp is a well known model to study male polymorphism [16–25]. Intense sexual selection in fig wasps has lead to competing males evolving exaggerated morphologies to mate, thereby enhancing their reproductive success . Beside winged males that tend to look similar to winged females, wingless males of fig wasps exhibit at least five different morphs . In some cases males are so different from females that they were initially described as different species or even genera . Wingless males engage in lethal combat for access to females [16, 29, 30], and consequently exhibit also considerable morphological diversity [20, 25], whereas winged males disperse to mate with females outside the syconium.
Fig wasps are a polyphyletic assemblage of Chalcidoidea (and few braconids) that develop in the inflorescences of fig trees (Moraceae, Ficus). They are subdivided into pollinating fig wasps that belong to the family Agaonidae (but see [31, 32]) and non-pollinating fig wasps (NPFW) that comprise, among others, five unrelated subfamilies strictly associated with Ficus: Otitesellinae, Sycoecinae, Sycoryctinae, Epichrysomallinae and Sycophaginae .
NPFW are associated with almost all the ca 750 Ficus species worldwide. They form rich communities of interacting species (up to 36) that differ among regions of the world and among groups of Ficus. The genus Ficus is characterized by its unique enclosed inflorescence, the syconium, which is an urn-shaped receptacle that contains tens to thousands flowers. Most species of NPFW oviposit from outside the syconium but some species enter receptive syconia through a narrow tunnel called the ostiole and oviposit in the flowers from inside the syconium. The biology of NPFW are poorly known and few detailed studies have reported reliable observations. Among these five groups of fig wasps, the subfamily Sycophaginae stand out as the most diverse in terms of male morphologies, biology and galling habits as well as timing of oviposition.
Taxonomy, diversity and distribution of sycophagine fig wasps
For a long time, Sycophaginae was considered to be the only subfamily of NPFW [33–35]. The classification and the taxonomic limits of the group have been modified several times. Sycophaginae have been classified as a subfamily of Torymidae [35–38], or of Agaonidae . Presently, they are not assigned to a family  and their phylogenetic position within Chalcidoidea remains unknown .
Taxonomy, distribution and life-history stategies of sycophagine fig wasps.
Before pollination (group 1)
shorter than body length
After pollination (group 5)
equal or longer than body length
Before & during pollination (groups 1 & 3)
shorter than body length
Idarnes group incerta
Before pollination (group 1)
shorter than body length
Idarnes group flavicollis
After pollination (group 2)
longer than body length
Idarnes group carme
After pollination (group 4)
longer than body length
Afrotropical + Oriental + Australasian
Before & After pollination (groups 1, 2, 4 & 5)
Shorter, equal to or longer than body length
Before pollination (group 1)
shorter than body length
During pollination (group 3)
shorter than body length
Extrapolating from our sampling of several hundred species of Ficus species the total diversity of the Sycophaginae could reach 700 species worldwide. Consequently, more than 80% of the species of Sycophaginae probably await description (Figure 2). The subfamily occurs in all tropical regions of the world and is associated with two unrelated subgenera of Ficus, namely Urostigma and Sycomorus [44, 45] (Table 1). More precisely, Neotropical Idarnes and Anidarnes wasps develop in Ficus of section Americana (subgenus Urostigma), Australasian Eukoebelea are strictly associated with Ficus section Malvanthera (subgenus Urostigma), Oriental Conidarnes are associated with section Conosycea (subgenus Urostigma); while Apocryptophagus and Sycophaga species are associated with the fig trees of the subgenus Sycomorus, with the exception of two species of Apocryptophagus found on F. orthoneura (subgenus Urostigma, section Urostigma) in Southern China during this study.
Only a few species-level phylogenies of Sycophaginae have been published so far, mostly on Papuan Apocryptophagus [46, 47] and Neotropical Idarnes . A recent study by Cruaud et al.  reconstructed the historical biogeography of Sycophaginae and proposed a first hypothesis based on 55 species. However, the authors focused neither on the taxonomy of the group nor on the evolution of the life-history strategies.
Biology of sycophagine fig wasps
Sycophaginae use chemical mediation to locate fig trees . Up to 6 species of Sycophaginae can be found on the same Ficus species [50–55]. In most cases, these small communities are structured by the development of the syconium, the timing of oviposition (before, during or after pollination) and the feeding habits of the wasps (gallers vs. cleptoparasites) [50, 51, 56]. The biology of sycophagine NPFW remains poorly known and extremely difficult to ascertain. For some species, trophic status is inferred by oviposition behaviour and biology remains suspected.
Group 1. The large gall-inducers
This group contains wasps much larger than the co-occurring pollinators. They exhibit relatively shorter and thicker ovipositor (shorter than their body length) than other Sycophaginae ovipositing in the same syconia. They oviposit through the syconium wall seven to 12 days before pollination (data on one species of the Idarnes incerta species group  and two species of Apocryptophagus [21, 59]). The number of galls per syconium is variable . However, the published data showed that there is a good correlation between large galls and small brood size (i.e. the mean number of conspecific sycophagine wasps developing per syconium [61–63]).
The large gall-inducers induce large galls that protrude into the syconium cavity and can occupy nearly the entire volume of the syconium . There is no documentation of any such large wasps possessing an ovipositor as long as that of small gall-inducers ovipositing on the same syconium. This suggests that this group is biologically homogeneous. This ecology occurs within several genera: Anidarnes (all species), Conidarnes (most), Pseudidarnes (all), Idarnes incerta species group (all) and Apocryptophagus (a few).
Group 2. The small gall-inducers
These smaller wasps (about the same size than the co-occuring pollinators), exhibit medium to long and thin ovipositor (longer than body length). They oviposit into the fig flowers a few days before fig pollination (direct demonstration for A. testacea ) or during fig pollination (direct demonstration for A. fusca ) and induce galls about the same size as those of the pollinator. At least one species of Idarnes is known to insert its ovipositor through the stigma and the style, and deposit its eggs between the inner integument and the nucellus , in this sense their oviposition is similar to that of the pollinators (although they oviposit from the outside). This type of gall-inducer occurs in many syconia at medium to high numbers and there are often many species of wasps per fig tree species. Available data on this group are still scarce but on average their brood sizes are medium to large [52, 54, 62, 64], although some species appear to have relatively small brood size (e.g. Apocryptophagus fusca . These species frequently exhibit different ovipositor lengths relative to their body size . They belong to the genus Apocryptophagus (most, experimental demonstration for two species ) and to the Idarnes flavicollis group (possibly all, experimental demonstration for one species ). Experimental demonstration consisted of introductions of females into bagged syconia and determining if wasps could develop independently of the presence of other fig wasps. This experiments ascertained the ability of these sycophagine wasps to induce galls.
Group 3. The ostiolar gall-inducers
This group contains species - about the same size as the co-occurring pollinators - that enter the syconia through the ostiole to oviposit, just like the pollinators do. Sycophaga sycomori drives its ovipositor along and not through the style of the flower (short or long-styled) and generally deposits one egg into the embryo sac . They frequently occur in the syconia and have relatively large brood sizes [52, 66]. The Afrotropical genus Sycophaga and possibly also one undescribed oriental species of Conidarnes (JYR, pers. obs.) belong to this group. Ostiolar gallers are apparently absent from the Neotropics.
Group 4. The cleptoparasites
In the fig wasp literature the terms "inquiline"  and "cleptoparasite" [57, 68] are sometimes used loosely as synonyms. Here we follow Gordh and Headrick (2001)  who defined the two words differently: an inquiline is a species that lives as a "guest" of another species but does not harm the host species. This happens in Cynipid wasps in which the development of the inquiline in a host gall is not always lethal to the gall inducer . A cleptoparasite is a parasite that preferentially attacks a gall already parasitized by another species of parasite. Consequently, cleptoparasites have always strong detrimental effect on the reproductive success of their host. Here we use the term "cleptoparasites" to refer to several groups of Sycophaginae for which detrimental effects on the reproductive success of the pollinators have been observed [52, 53]. As we have no direct evidence of sycophagine species developing as a strict parasite of another wasp larvae, the term "parasitoid" is not used here.
This fourth group is composed of smaller wasps (about the size of the co-occurring pollinators) that exhibit relatively longer ovipositor compared to other Sycophaginae occurring in the same fig trees (in average the ovipositor is much longer than the body length). They lay eggs one to three weeks after pollination (Idarnes, ). In the few documented cases, these wasps oviposit in galls induced by other fig wasp species and containing mostly larva of the pollinator (sometimes also of NPFW) [54, 65, 67]. They insert their ovipositor through the gall pericarp (Idarnes carme group), deposit their egg inside the embryo sac and the larva consumes the endosperm as well as the gall-maker larva . These species have relatively large brood size . At least in one species, when the parasitic pressure is high, the wasps (Idarnes) can oviposit in developing seeds and produce small males . These wasps belong to the genus Apocryptophagus (some, direct demonstration for A. agraensis ) and to Idarnes carme group (all, observation of timing of oviposition for one species ).
Group 5. The late "gall-inducers"
Some species groups generally recognized as gall-inducers oviposit after fig pollination. It is difficult to understand how these wasps that oviposit well after pollination could be gall-inducers. Indeed female flowers wither rapidly after pollination  suggesting that they can no longer be galled. Further, space constraints due to the tight internal flower packing resulting from ovule growth following pollination, probably prohibit new galls from developing . Hence these species are probably cleptoparasites, parasitoids of the gall-maker larva or even hyperparasitoids . They are abundant and, for the few studies published, seem to have relatively large brood size . Nevertheless, for conservative reasons we will call them late gall-inducers, pending further biological data. The members of this group belong to the genera Eukoebelea (all ?) and Apocryptophagus (some species ).
Sycophaginae exhibit extreme male polymorphism (Table 1). Some species-groups or genera have only winged males (Idarnes incerta group, Anidarnes, Conidarnes), some have dimorphic males (both winged and apterous individuals co-occur in the same syconia, e.g. Eukoebelea and Pseudidarnes), whereas the others have only wingless males (Sycophaga, Apocryptophagus, other groups of Idarnes).
Hamilton  proposed that sexual selection on male mating opportunities favored winged males in NPFW species with small broods and wingless males in species with large broods. Indeed, in species with large broods, females develop in syconia with conspecific males and mate before dispersal. Consequently, most mating opportunities are preempted by the apterous males particularly adapted to crawl between the flowers and mate within the syconium cavity. In contrast, in species with small broods, most females emerge into the syconium cavities in the absence of conspecific males, and winged males that are able to leave their natal syconium enjoy most mating opportunities .
Aims of the study
As mentioned above, sycophagine are ecologically diverse and show some of the most extreme examples of male dimorphism among fig wasps. Therefore, they appear to be good candidates to test Hamilton's hypothesis predicting that the presence of winged males and small brood size may be correlated. In this paper we employ multiple genetic loci, extensive taxon sampling over all known genera, and several different analytical approaches to discuss the generic delimitation and reconstruct the evolutionary history of sycophagine fig wasps. We then use this phylogeny to reconstruct the evolution of sycophagine life-history strategies (galling habits/brood size and male polymorphism) based on information compiled from our own observations and several literature sources [50–54, 60, 61, 63, 65, 73–76]. In this context, we propose hypotheses that could explain the observed male dimorphism within sycophagine fig wasps.
The final matrix contained 73 ingroup species and 4 outgroups, represented by 145 individuals and 4267 bp (COI+Cytb = 2231 bp, EF = 516 bp, 28S stems = 933 bp, 28S loops = 587 bp). Of these, 2307 bp were variable and 1984 bp were parsimony informative. Alignment of exons revealed no indels. For all partitions the GTR+I+G was determined as the best-fitting model by MrAIC.
Sycophaginae were divided in three well-supported clades: (i) the Australasian Eukoebelea; (ii) a clade comprising the Australasian Pseudidarnes, the Neotropical Anidarnes and the Oriental Conidarnes and (iii) a clade clustering Apocryptophagus and Sycophaga from the Old World and the Neotropical Idarnes. In all analyses, Eukoebelea was sister to all other Sycophaginae (PP 1.0, BP 99) and Pseudidarnes was basal to Anidarnes+Conidarnes (PP 0.99, BP 100).
Within the genus Idarnes, all three recognized species groups (namely incerta, flavicollis and carme) were recovered as monophyletic with strong support (PP 1.00, BP 100). The Idarnes carme group was basal to a clade clustering the Idarnes incerta and flavicollis groups (PP 1.00, BP 100).
Within the Apocryptophagus /Sycophaga clade, the internodes were short, making the recovery of unambiguous phylogenetic information difficult. The basal node was a polytomy of four groups: (i) Apocryptophagus species associated with section Sycocarpus; (ii and iii) two continental Asiatic species that are respectively associated with F. oligodon and F. orthoneura and (iv) all the remaining Apocryptophagus and all Sycophaga species. The first group was recovered basal to the other 3 groups but this relationship was only supported in the Bayesian reconstruction (PP 0.96, BP 65). In the fourth group, the remaining Apocryptophagus and the Sycophaga species were distributed in five well-supported clades with no firmly established order of branching: (i) the Apocryptophagus species associated with Adenosperma fig trees (PP 1.00, BP 100); (ii) one Apocryptophagus species associated with F. prostata; (iii) a group including A. agraensis and A. spinitarsus, two cleptoparasites associated with F. racemosa and F. variegata respectively; (iv) a small group of Apocryptophagus species associated with F. variegata and F. nodosa; (v) a large and well supported clade (PP 0.99, BP 99) of Apocryptophagus and Sycophaga species exclusively associated with monoecious Sycomorus fig trees. Clade (v) was further subdivided into four groups: (i) the Afrotropical Apocryptophagus species with extremely long ovipositors associated with F. sur, F. vallis-choudae and F. sycomorus; (ii) an Apocryptophagus species with a long ovipositor associated with F. sur; (iii) a well supported clade grouping two species of early gallers, namely A. gigas associated with F. sycomorus and F. mucuso and A. stratheni associated with F. racemosa; (iv) a polytomy of three groups comprising A. testacea and A. fusca which were sister species; a well supported clade including all Sycophaga species; and a well supported and fully resolved clade grouping all species of Apocryptophagus from Madagascar and the Mascarene islands.
Evolution of life history strategies
The ancestral biology of Sycophaginae is also ambiguous. Large gall-inducers, laying eggs in the syconium before pollination, medium-sized gall-inducers ovipositing during pollination or late gall-inducers ovipositing after pollination were equiprobable (proportional likelihoods of 0.21, 0.39 and 0.18, respectively). The ancestor of the clade Idarnes + Apocryptophagus/Sycophaga was a medium sized gall-inducer that oviposited from outside at the same time as the pollinator (proportional likelihood of 0.87). Entrance through the ostiole appeared once and the ancestor was a medium-sized gall-inducer ovipositing from the outside during pollination (proportional likelihoods of 0.85). Cleptoparasitism appeared independently in the Idarnes carme group and in the genus Apocryptophagus. In both cases this new biology evolved from medium-sized gallers that oviposit from the outside of the syconium during pollination (proportional likelihoods of 0.86 and 0.95 respectively). The ability to develop large galls before pollination, whatever the ancestral biology, appeared at least four times independently (twice within Apocryptophagus) illustrating the lability of such biology.
We employed multiple genetic loci, extensive taxon sampling, and several different analytical approaches to reconstruct the evolutionary history of the Sycophaginae. The resulting topology is well resolved and provides strong support for most notable relationships within the subfamily. Our phylogeny generally agrees with a recent molecular study using more limited taxon sampling . Therefore, we propose to lay the foundation for a revised classification of the subfamily.
Our analyses highlight three main clades that could be treated as tribes: (i) the Australasian Eukoebelea; (ii) the Australasian Pseudidarnes, the Neotropical Anidarnes and the Oriental Conidarnes and (iii) the Old World Apocryptophagus and Sycophaga, and the Neotropical Idarnes.
Our phylogeny strongly suggests Eukoebelea as the basal taxon within Sycophaginae. This is corroborated by several morphological characters: 1) the linear notauli without transverse crenulation (Figure 1A), 2) the absence of a well-delimitated supraclypeal area (Figure 1K), 3) both palpi (maxillary and labial) one-segmented, 4) the propodeal spiracles separated from the fore margin of the propodeum (Figure 1A) and 5) a long pronotum.
The phylogenetic reconstruction gives strong support for a Pseudidarnes + Anidarnes + Conidarnes clade, an alliance that has never been proposed by any of the previous taxonomic studies (but see ). This group is however well defined and characterized by 1) the deep and transversely crenulated notauli, axillular and frenal grooves (Figure 1D), all of them conspicuous; 2) the antennae inserted high on the face (Figure 1H); 3) the dorsellum conspicuous (Figure 1D); 4) the clypeal margin bilobed (Figure 1H).
Idarnes and Apocryptophagus/Sycophaga are sister taxa. This clade is difficult to define morphologically due to the morphological adaptations exhibited by the ostiolar Sycophaga and by the morphological characters of the Idarnes incerta species group. However, the group is characterized by at least some of the following characters: 1) short pronotum (except in ostiolar Sycophaga) (Figure 1B), 2) notauli deep and transversely crenulated (Figure 1B) (except in ostiolar Sycophaga, Figure 1E), 3) furcal pit enclosed within the mesosternum, 4) the clypeal margin straight or emarginated (Figure 1G) and 5) labial palpi two-segmented.
Anidarnes and Idarnes are never recovered in a single clade, which means that communities associated with Neotropical fig trees comprise two unrelated genera of Sycophaginae. Therefore, Sycophaginae communities associated with Americana fig trees in South America could be the result of two independent colonisations (see ).
Anidarnes is recovered as monophyletic. However, the inference of intra generic relationships requires more extensive sampling. Idarnes is monophyletic and the incerta group is deeply nested within the genus. Consequently and despite clear morphological differentiation from the two other species groups that share more characters, the incerta group does not represent a distinct genus as suggested by Rasplus and Soldati .
Females of Sycophaga are strongly differentiated and exhibit several apomorphies, such as a flattened and elongated head (Figure 1J), a short fore tibiae bearing teeth (Figure 1C), numerous spurs on the hind tibia (Figure 1F) and an absence of any sculpture on the mesosoma (Figure 1E). All these characters probably evolved as adaptations for crawling through the ostiole and make them easy to identify. A phylogeny based on morphological characters of females would probably support Sycophaga and Apocryptophagus as distinct lineages. However, in all our analyses, Sycophaga makes Apocryptophagus paraphyletic, with strong support. This result based on a molecular approach is corroborated by male morphology (Figure 1I). Indeed, males of Apocryptophagus and Sycophaga cannot be separated on tangible morphological characters. They exhibit a unique suite of synapomorphies (Figure 1): 1) the long peritremata of the abdominal spiracles (Figure 1I) that prevent the entry of water contained in the cavity of mature Sycomorus syconia into the tracheae and allow respiration [77, 78], 2) the flat scape of the antenna and 3) the rectangular head (Figure 1I). Therefore, as already suggested by Bouček , we propose to consider Apocryptophagus as a junior synonym of Sycophaga (syn. nov). Hereafter, all Apocryptophagus species will be named under Sycophaga.
Evolution of life history strategies
Our analyses show that the two investigated traits (galling habits/brood sizes and male polymorphism) are evolutionarily dynamic (Figure 6). Although the ancestral states for the whole subfamily cannot be firmly established, we highlight some interesting results.
Based on currently accepted biology of extant species, the ancestor of Sycophaginae was probably a galler. However, if Eukoebelea species were in fact cleptoparasites, as suggested in the introduction, then the feeding regime of the ancestor would be ambiguous. During sycophagine diversification, the same biologies re-evolved several times independently in distantly related lineages. Indeed, the ability to induce large galls evolved at least four times independently (Anidarnes + Pseudidarnes + Conidarnes clade, Idarnes incerta group and some Apocryptophagus species). Cleptoparastism evolved independently in Idarnes and Apocryptophagus. However, biological observations are missing for many sycophagine species and we cannot discard the possibility that cleptoparasitism also appeared in some other clades (four-five times if late gallers are in fact cleptoparasites). Clearly, more direct studies of larval ecology through the dissection of galls, observation of larval habits or experimental introductions and exclusions are needed. Life history strategies including whether sycophagine species are gallers or parasitic on other fig wasp larvae have only been demonstrated for few species based on field observations and experimental introductions (e.g. [50, 52, 54]. However, even careful experimental introductions and exclusions have limitations. For example, it is not possible to discriminate between species that oviposit early in the host galls and mostly feed on plant rather than insect tissue and wasps that oviposit later (once the gallmaker larva is fully developed) and mostly feed on insect tissue.
The ability to enter the syconia through the ostiole appeared only once (one lineage of Sycophaga, in our extended definition of the generic limits). Given that sycophagine fig wasps probably originated 50-40 Ma and following the Sycophaga stem group estimates from Cruaud et al.  (20-10 Ma), this biology took about 30 Ma to evolve. In the next 15 Ma, females evolved several morphological adaptations to crawl through the bracts that make their morphology strongly divergent from their sister lineages (see previous paragraph for details). It is noteworthy that one undescribed species of Conidarnes could also be an ostiolar gall-inducer. However, this hypothesis is based only on the external morphology of the species (JYR, pers. obs.) and needs to be confirmed by further field observations. Confirmation of this hypothesis would imply that the ability to enter the syconia appeared twice independently.
The evolutionary lability of male morphology is typical of traits that experience strong sexual selection, and has also been described for horns in scarabaeid beetles . Within the sycophagines, winged and wingless morphs are distinguished not just by whether they have wings, but also by their behaviour (winged males can disperse outside the syconia whereas wingless males can fight and compete for females within the syconia) and important morphological differences. Winged males resemble their conspecific females closely, but wingless males are so divergent in form that they have repeatedly, and mistakenly, been classified in different taxa. They exhibit large mandibles, flattened head with small eyes and have fused mesosoma segments (Figures 4, 5). The occurrence of winged males, wingless males or both is highly labile across the phylogeny and closely related species can be monomorphic for opposite wing morphologies. For example, species in the Idarnes incerta group have winged males whereas all other Idarnes species produce apterous males. This polymorphism has also been observed in other groups such as aphids , ants  and parasitoid wasps  and suggests lability in the developmental processes leading to morph determination.
Our analyses indicate positive evolutionary correlations between large gall types/small brood sizes and winged males (Anidarnes + Pseudidarnes + Conidarnes clade and Idarnes incerta group all belonging to group 1 (Figure 6), a result that was previously reported for other NPFWs  and supports Hamilton's hypothesis . Pseudidarnes minerva is the only exception known to date . This Australian species associated with Malvanthera fig trees has both polymorphic males (wingless and winged morphs) and small broods. The wingless males do not fight, but dig a hole and and enter galls containing females to mate. This peculiar behaviour could explain why male polymorphism is retained in this species. In contrast, our analyses reveal that species with large brood size tend to produce wingless males.
We could not ascertain the ancestral morph. The proportional likelihood of aptery (0.84) is higher than the likelihood of the winged condition (0.15) but the difference is not significant. Therefore two scenarios can be proposed: 1) a basal loss of winged morphs and their later re-evolution and 2) a winged ancestor and the independent and recurrent losses of the winged morph in several lineages. In both cases, winged or wingless morph reappearance would be linked with galling strategy. It is noteworthy that the few Apocryptophagus species that produce large galls long before the pollinators (e.g. A. gigas and A. stratheni, some species associated with Sycomorus fig trees) exhibit wingless males only. These species have not re-evolved winged morphs even at very low densities . This interesting result could be explained by other selective pressures that strongly counterselect the re-appearance of winged morphs. In particular, the presence of liquid in the syconia could explain why the males remain apterous. Indeed, in fig trees belonging to the Sycomorus subgenus, the cavity of the syconia can be filled with watery liquid during the interforal and male phases of the syconium development. Consequently, males emerge from their galls at a time when the galls are still covered by a thin film of liquid and literally swim between the galls during their search for mates . Complementary explanations may include the large size of Sycomorus syconia in terms of numbers of flowers compared to that of other species groups hosting Sycophaginae. This could allow a higher number of large galls to develop within a single syconium , increasing the brood size of the species.
The resulting trees from our phylogenetic analyses are mostly well resolved and highly supported. Therefore, we provide here an accurate phylogenetic framework that can be used in comparative evolutionary and ecological studies using Sycophaginae as a model system. Additional work is still needed on Sycophaginae phylogenetics. Indeed, our sampling represents only 10% of the estimated biodiversity of the group. Moreover, the relationships within Sycophaga (in our extended definition of the generic limits) are still somewhat uncertain. We also show that life history strategies of Sycophaginae are evolutionary labile, such that distantly related taxa share similar galling habits and vice-versa. The reconstruction of patterns of evolution of male polymorphism between or within the Sycophaginae genera suggests a lability in the developmental processes underlying the male morphology determination. Moreover, we show that winged males are favored in species that induce few galls within the syconia and wingless males are favored in species with large brood sizes. However, our results have to be taken with caution given that our inferences are based on a few number of studies dealing with sycophagine biology. Some species appear exceptional in that they apparently induce few galls but exhibit apterous males, which we hypothesize could be due to other factors that strongly select against the re-appearance of winged morphs (presence of liquid in the syconium, large number of flowers, etc). Untangling the full diversity of the biology of Sycophaginae will involve a tremendous amount of field work. What we have put forward here are a series of propositions on what is the biology of the species and how it evolved. Though preliminary in nature and although some propositions might be challenged by future work, this is the first proposal on how the biology of a group of non pollinating fig wasps diversified. Even within the current, limited, state of knowledge, we can firmly claim that we observe surprisingly abundant cases of repeated independent evolution of similar biologies. The Sycophaginae thus constitute an interesting model to test predictions on what factors will canalize the evolution of particular biologies and morphs.
Taxonomic and gene sampling
We expanded the sampling of our previous study  and included 73 ingroup species, 93% of which were represented by two individuals. All known genera of Sycophaginae as well as most species-groups were represented totaling about 1.5 times the number of described species (Additional file 1 Table S1, Figure 2). As phylogenetic relationships within Chalcidoidea are still unresolved, closer and more distant relatives were included as outgroups . Five species belonging to the genera Ceratosolen (Agaonidae), Odontofroggatia (Epichrysomallinae), Ficomila (Eurytomidae) and Megastigmus (Torymidae) were used. All material was collected alive and fixed in 95% ethanol. Each time destructive extraction was used, vouchers were selected among specimens sampled from the same fig tree and the same syconium after carefull identification. Vouchers are deposited at CBGP, Montferrier-sur-Lez, France. A high definition image library of vouchers was also constructed, using EntoVision Premium Portable Imaging System, to allow future identification of specific taxa and traceability of our results (see Figures 4, 5 for examples). In the present study we combined one nuclear protein-coding gene (F2 copy of elongation factor-1a, EF-1a), two mitochondrial protein-coding genes (Cytochrome oxydase I, COI and Cytochrome b, Cytb) and 28S rRNA (D2-D3 and D4-D5 expansion regions). Extraction, PCR and sequencing protocols follow . Both strands for each overlapping fragment were assembled using the sequence editing software Geneious v3.7 . All the sequences were deposited in GenBank (Additional file 1, Table S1).
Protein-coding genes (COI, Cytb, EF) were aligned using ClustalW 1.81  with default gap opening, extension and substitution costs. For confirmation, alignments were translated to amino acids using MEGA version 4 . Alignment of sequences encoding rRNA was based on secondary structure models [87, 88] using the terminology developed by Kjer  and Gillespie et al.. The structural model of rRNA fragments and alignment details follow Cruaud et al. .
Because our dataset comprised protein-coding genes and rRNA, we performed partitioned analyses implementing separate nucleotide substitution models for subsets of the data more likely to have experienced similar evolutionary processes (mitochondrial genes, EF and rRNA stems and loops). Best fitting model for each partition was identified using the Akaike information criterion  as implemented in MrAIC.pl 1.4.3.
Phylogenetic trees were estimated using maximum likelihood (ML) and Bayesian methods and all the analyses were conducted on a 150 cores Linux Cluster at CBGP.
We performed ML analyses and associated bootstrapping using the MPI-parallelized RAxML 7.0.4. . GTRCAT approximation of models was used for ML boostrapping  (1000 replicates). Bootstrap percentage (BP) > 95% was considered as strong support and a BP < 70% as weak.
Bayesian analyses were conducted using a parallel version of MrBayes v. 3.1.1. (Huelsenbeck & Ronquist, 2001). We assumed across-partition heterogeneity in model parameters by considering the parameter m. Parameter values for the model were initiated with default uniform priors and branch lengths were estimated using default exponential priors. To improve mixing of the cold chain and avoid it converging on local optima, we used Metropolis-coupled Markov chain Monte Carlo (MCMC), with each run including a cold chain and three incrementally heated chains. The heating parameter was set to 0.02 in order to allow swap frequencies from 20% to 70%. We ran two independent runs of 10 million generations. All values were sampled every 1000 generations. For the initial determination of burn-in, we examined the plot of overall model likelihood against generation number to find the point where the likelihood started to fluctuate around a constant value. The points sampled prior to convergence of the chains were then discarded. We used a range of MCMC convergence and good mixing diagnostics following Cruaud et al.. The results were based on the pooled samples from the stationary phases of the two independent runs. Posterior probabilities (PP) > 0.95 were considered as strong support.
Evolution of life-history strategies
We compiled information on galling habits/brood size and male polymorphism of each sampled species from several literature sources [50–54, 60, 61, 63, 65, 73–76] and from few unpublished observations made by us. We considered three different states for male polymorphism: 0: winged; 1: dimorphic (both winged and wingless males); 2: apterous. We used five different states to describe the biology of the Sycophaginae: 0: large gallers laying eggs in the syconium before pollination; 1: small-sized gallers ovipositing during pollination; 2: small-sized gallers ovipositing after pollination (late "gall-inducers"); 3: ostiolar galler ovipositing during the pollination and 4: cleptoparasites ovipositing long after pollination.
To infer the evolution of life-history strategies, we conducted Maximum Parsimony and ML ancestral state reconstructions using Mesquite 2.73 . All reconstructions were performed on the ML topology. ML reconstructions were conducted using a single-rate Mk likelihood model for discrete morphological characters , which assumes that any particular change is equally probable. ML takes branch lengths into account and allows quantifying the uncertainty associated with each reconstructed ancestral state . The likelihood decision threshold, i.e. the minimum difference in likelihood between the best and the next best state needed for assigning a state to a node was set to 2.0 .
"To our friend Jean-Noël Labat, professor at MNHN Paris, France, who guided us during our first trip in Madagascar in 1996 and died too young" JYR and CK.
We thank Dominique Strasberg (La Réunion, France) and William Ramirez (San José, Costa Rica) for contributing samples. We also thank all our guides in Borneo, Sulawesi, Papua Barat and Gabon, especially Jaman, Lary and Mado. We also thank Alexandre Dehne Garcia and Arnaud Estoup (CBGP, Montpellier) for their help with cluster computers and John Heraty and James Munroe (University of California, Riverside) for their advice on alignment and their provision of alignment framework for Chalcidoidea. Special thanks to Emmanuelle Jousselin (CBGP, Montpellier) for valuable discussion and help. Financial support was provided by grants from the ANR (National Research Agency) that supports the 'NiceFigs' and "BioFigs" projects, led by Martine Hossaert-McKey (CNRS, Montpellier, France) and Lien Siang Chou (National Taiwan University, Taipei, Taiwan), Biota/Fapesp (04/10299-4) and CNPq (302769/2008-0) that support R.A.S.P., a fellowship FAPESP grant (07/06054-4) to F.H.A.F. and an NRF grant GUN 61497 to S.v.N. We thank the anonymous reviewers for their careful read and thoughtful comments on previous version of this article.
- Gross MR: Alternative reproductive strategies and tactics: diversity within sexes. Trends in Ecology and Evolution. 1996, 11: 92-98. 10.1016/0169-5347(96)81050-0.View ArticlePubMed
- Shuster SM, Wade MJ: Mating Systems and Strategies. 2003, Princeton, New Jersey: Princeton University Press
- Andersson M: Sexual Selection. 1994, Princeton, New Jersey: Princeton University Press
- Danforth BN: The morphology and behavior of dimorphic males in Perdita portalis (Hymenoptera, Andrenidae). Behav Ecol Sociobiol. 1991, 29 (4): 235-247. 10.1007/BF00163980.View Article
- Oettler J, Suefuji M, Heinze J: The evolution of alternative reproductive tactics in male Cardiocondyla ants. Evolution. 2010, 64 (11): 3310-3317. 10.1111/j.1558-5646.2010.01090.x.View Article
- Romo-Beltran A, Macias-Ordonez R, Cordoba-Aguilar A: Male dimorphism, territoriality and mating success in the tropical damselfly, Paraphlebia zoe Selys (Odonata: Megapodagrionidae). Evol Ecol. 2009, 23 (5): 699-709. 10.1007/s10682-008-9265-1.View Article
- Tomkins JL, Brown GS: Population density drives the local evolution of a threshold dimorphism. Nature. 2004, 431 (7012): 1099-1103. 10.1038/nature02918.View ArticlePubMed
- Emlen DJ, Hunt J, Simmons LW: Evolution of sexual dimorphism and male dimorphism in the expression of beetle horns: Phylogenetic evidence for modularity, evolutionary lability, and constraint. Am Nat. 2005, 166 (4): S42-S68. 10.1086/444599.View ArticlePubMed
- Vanacker D, Vanden Borre J, Jonckheere A, Maes L, Pardo S, Hendrickx F, Maelfait JP: Dwarf spiders (Erigoninae, Linyphiidae, Araneae): good candidates for evolutionary research. Belg J Zool. 2003, 133 (2): 143-149.
- Buzatto BA, Requena GS, Lourenco RS, Munguia-Steyer R, Machado G: Conditional male dimorphism and alternative reproductive tactics in a Neotropical arachnid (Opiliones). Evol Ecol. 2011, 25 (2): 331-349. 10.1007/s10682-010-9431-0.View Article
- Radwan J, Klimas M: Male dimorphism in the bulb mite, Rhizoglyphus robini: fighters survive better. Ethol Ecol Evol. 2001, 13 (1): 69-79. 10.1080/08927014.2001.9522788.View Article
- Conlan KF: Male dimorphism and delay in sexual development in the colonial amphipod Jassa - response to sexual selection by inference. Am Zool. 1987, 27 (4): A35-A35.
- Ainsworth R: Male dimorphism in 2 new species of nematode (Pharyngodonidae, Oxyurida) from new-zealand lizards. J Parasitol. 1990, 76 (6): 812-822. 10.2307/3282799.View Article
- Corl A, Davis AR, Kuchta SR, Comendant T, Sinervo B: Alternative mating strategies and the evolution of sexual size dimorphism in the side-blotched lizard, Uta stansburiana: a population-level comparative analysis. Evolution. 2010, 64 (1): 79-96. 10.1111/j.1558-5646.2009.00791.x.View ArticlePubMed
- Hurtado-Gonzales JL, Uy JAC: Intrasexual competition facilitates the evolution of alternative mating strategies in a colour polymorphic fish. BMC Evol Biol. 2010, 10:
- Greeff J, van Noort S, Rasplus J-Y, Kjellberg F: Dispersal and fighting in male pollinating fig wasps. C R Biol. 2003, 326: 121-130. 10.1016/S1631-0691(03)00010-6.View ArticlePubMed
- Murray MG: Environmental constraints on fighting in flightless male fig wasps. Anim Behav. 1989, 38: 186-193. 10.1016/S0003-3472(89)80081-8.View Article
- Bean D, Cook J: Male mating tactics and lethal combat in the nonpollinating fig wasp Sycoscapter australis. Anim Behav. 2001, 62: 535-542. 10.1006/anbe.2001.1779.View Article
- Herre EA, West SA, Cook JM, Compton SG, Kjellberg F: Fig-associated wasps: pollinators and parasites, sex-ratio adjustment and male polymorphism, population structure and its consequence. The Evolution of Mating Systems in Insects and Arachnids. Edited by: Choe JC, Crespi BJ. 1997, Cambridge, UK: Cambridge University Press, 226-239.View Article
- Jousselin E, van Noort S, Greeff JM: Labile male morphology and intraspecific male polymorphism in the Philotrypesis fig wasps. Mol Phylogenet Evol. 2004, 33: 706-718. 10.1016/j.ympev.2004.08.008.View ArticlePubMed
- Moore JC, Pienaar J, Greeff JM: Male morphological variation and the determinants of body size in two Otiteselline fig wasps. Behav Ecol. 2004, 15 (5): 735-741. 10.1093/beheco/arh069.View Article
- Pienaar J, Greeff JM: Different male morphs of Otitesella pseudoserrata fig wasps have equal fitness but are not determined by different alleles. Ecol Lett. 2003, 6 (4): 286-289. 10.1046/j.1461-0248.2003.00441.x.View Article
- Niu LM, Hu HY, Huang DW, Fu YG, Peng ZQ: Brood size: a major factor influencing male dimorphism in the non-pollinating fig wasp Sycobia sp. Ecol Entomol. 2009, 34 (6): 696-701. 10.1111/j.1365-2311.2009.01123.x.View Article
- Moore JC, Obbard DJ, Reuter C, West SA, Cook JM: Male morphology and dishonest signalling in a fig wasp. Anim Behav. 2009, 78: 147-153. 10.1016/j.anbehav.2009.04.006.View Article
- Cook JM: Alternative mating tactics and fatal fighting in male fig wasps. Insect Evolutionary Ecology. Edited by: Fellowes MDE, Holloway GJ, Rolff J. 2005, Royal Entomological Society, 83-109.
- Frank JH: Weapons and fighthing in fig wasps. Trends in Ecology and Evolution. 1987, 2 (9): 259-260. 10.1016/0169-5347(87)90029-2.View Article
- Murray MG: Comparative morphology and mate competition of flightless male fig wasps. Anim Behav. 1990, 39: 434-443. 10.1016/S0003-3472(05)80406-3.View Article
- Mayr G: Feigeninsecten. Verhandlungen der Zoologisch-Botanischen Gesellschaft in Wien. 1885, 35: 147-250.View Article
- Cook JM, Bean D: Cryptic male dimorphism and fighting in a fig wasp. Anim Behav. 2006, 71: 1095-1101. 10.1016/j.anbehav.2005.07.027.View Article
- Reinholdt K: Influence of male relatedness on lethal combat in fig wasps: a theoretical analysis. Proceedings of the Royal Society of London B. 2003, 270 (1520): 1171-1175. 10.1098/rspb.2003.2368.View Article
- Jousselin E, Rasplus JY, Kjellberg F: Shift to mutualism in parasitic lineages of the fig/fig wasp interaction. Oikos. 2001, 94: 287-294. 10.1034/j.1600-0706.2001.940209.x.View Article
- Peng YQ, Duan Z-B, Yang DR, Rasplus JY: Co-occurrence of two Eupristina species on Ficus altissima in Xishuangbanna, SW China. Symbiosis. 2008, 45: 9-14.
- Bouček Z: Australasian Chalcidoidea (Hymenoptera): a biosystematic revision of genera of fourteen families, with a reclassification of species. 1988, Wallingford, Oxon, UK: CAB International
- Walker F: Notes on Chalcidiae. Part 3. Torymidae and Chalcididae. 1871, 37-54.
- Wiebes JT: The structure of the ovipositing organs as a tribal character in the Indo-Australian Sycophaginae Torymidae (Hymenoptera, Chalcidoidea). Zoologische Mededelingen. 1966, 41: 151-159.
- Hill DS: Figs (Ficus spp.) and fig-wasps (Chalcidoidea). J Nat Hist. 1967, 1 (3): 413-434. 10.1080/00222936700770401.View Article
- Joseph KJ: A proposed revision of the classification of the fig insects of the families Agaonidae and Torymidae (Hymenoptera). Proceedings of the Royal Entomological Society of London (B). 1964, 33 (3/4): 63-66.
- Wiebes JT: A short history of fig wasps research. Gardens' Bulletin Singapore. 1976, 29: 207-232.
- Rasplus JY, Kerdelhué C, Le Clainche I, Mondor G: Molecular phylogeny of fig wasps (Hymenoptera). Agaonidae are not monophyletic. Compte Rendu de l'Académie des Sciences de Paris. 1998, 321: 517-527.
- Grissell EE: Toryminae (Hymenoptera: Chalcidoidea: Torymidae): a redefinition, generic classification and annotated world catalogue of species. Memoirs on Entomology, International. 1995, 2: 1-470.
- Gibson GAP: Family Agaonidae. Hymenoptera of the world: An identification guide to families. Edited by: Goulet H, Huber JT. 1993, Ottawa: Agriculture Canada Publication, 610-614.
- Boucek Z: The genera of chalcidoid wasps from Ficus fruit in the New World. J Nat Hist. 1993, 27: 173-217. 10.1080/00222939300770071.View Article
- Rasplus JY, Soldati L: Familia Agaonidae. Introducción a los Hymenoptera de la Región Neotropical. Edited by: Fernández F, Sharkey M. 2007, Instituto Humboldt and Sociedad Colombiana de Entomología, 683-698.
- Rønsted N, Weiblen GD, Cook JM, Salamin N, Machado CA, Savolainen P: 60 million years of co-divergence in the fig-wasp symbiosis. Proc R Soc Biol Sci Ser B. 2005, 272: 2593-2599. 10.1098/rspb.2005.3249.View Article
- Cruaud A, Jabbour-Zahab R, Genson G, Couloux A, Yan-Qiong P, Da Rong Y, Ubaidillah R, Pereira RAS, Kjellberg F, Van Noort S, et al: Out-of-Australia and back again: the worldwide historical biogeography of non-pollinating fig wasps (Hymenoptera: Sycophaginae). J Biogeogr. 2011, 38: 209-225. 10.1111/j.1365-2699.2010.02429.x.View Article
- Silvieus SI, Clement WL, Weiblen GD: Cophylogeny of figs, pollinators, gallers and parasitoids. Specialization, speciation, and radiation: the evolutionary biology of herbivorous insects. Edited by: Tilmon KJ. 2008, Berkeley, California: University of California Press, 225-239.
- Weiblen GD, Bush GL: Speciation in fig pollinators and parasites. Mol Ecol. 2002, 11: 1573-1578. 10.1046/j.1365-294X.2002.01529.x.View ArticlePubMed
- Marussich WA, Machado CA: Host-specificity and coevolution among pollinating and nonpollinating New World fig wasps. Mol Ecol. 2007, 16 (9): 1925-1946. 10.1111/j.1365-294X.2007.03278.x.View ArticlePubMed
- Proffit M, Schatz B, Borges RM, Hossaert-McKey M: Chemical mediation and niche partitioning in non-pollinating fig-wasp communities. J Anim Ecol. 2007, 76 (2): 296-303. 10.1111/j.1365-2656.2007.01213.x.View ArticlePubMed
- Elias LG, Menezes AO, Pereira RAS: Colonization sequence of non-pollinating fig wasps associated with Ficus citrifolia in Brazil. Symbiosis. 2008, 45 (1-3): 107-111.
- Kerdelhué C, Rasplus JY: Non-pollinating Afrotropical fig wasps affect the fig-pollinator mutualism in Ficus within the subgenus Sycomorus. Oikos. 1996, 75: 3-14. 10.2307/3546315.View Article
- Kerdelhué C, Rossi JP, Rasplus JY: Comparative community ecology studies on Old World figs and fig wasps. Ecology. 2000, 81 (10): 2832-2849.View Article
- West SA, Herre EA: The ecology of the New World fig-parasitizing wasps Idarnes and implications for the evolution of the fig-pollinator mutualism. Proceedings of the Royal Society B: Biological Sciences. 1994, 258: 67-72. 10.1098/rspb.1994.0143.View Article
- Wang RW, Zheng Q: Structure of a fig wasp community: temporal segregation of oviposition and larval diets. Symbiosis. 2008, 45 (1-3): 113-116.
- Nazareno AG, da Silva RBQ, Pereira RAS: Fauna of hymenoptera in Ficus spp. (Moraceae) in the Central Amazon, Brazil. Iheringia Ser Zool. 2007, 97 (4): 441-446. 10.1590/S0073-47212007000400013.View Article
- Compton SG, Rasplus JY, Ware AB: African fig wasp parasitoid communities. Parasitoid community ecology. Edited by: Hawkins BA, Sheehan W. 1994, Oxford: Oxford University Press, 323-348.
- Cook JM, Rasplus J-Y: Mutualists with attitude: coevolving fig wasps and figs. Trends Ecol Evol. 2003, 18 (5): 241-248. 10.1016/S0169-5347(03)00062-4.View Article
- Cook JM, Segar ST: Speciation in fig wasps. Ecol Entomol. 2010, 35: 54-66.View Article
- Peng Y-Q, Yang DR, Duang ZB: The population dynamics of a non-pollinating figwasp on Ficus auriculata, at Xishuangbanna, China. J Trop Ecol. 2005, 21: 581-585. 10.1017/S0266467405002634.View Article
- Godfray HC: Virginity in haplodiploid populations: a study on fig wasps. Ecol Entomol. 1988, 13: 283-291. 10.1111/j.1365-2311.1988.tb00358.x.View Article
- Cook JM, Compton SG, Herre EA, West SA: Alternative mating tactics and extreme male dimorphism in fig wasps. Proceedings of the Royal Society of London B. 1997, 264: 747-754. 10.1098/rspb.1997.0106.View Article
- West SA, Herre EA: Partial local mate competition and the sex ratio: a study on non-pollinating fig wasps. J Evol Biol. 1998, 11: 531-548.View Article
- Bronstein JL: Natural history of Anidarnes bicolor (Hymenoptera: Agaonidae), a galler of the Florida strangling fig (Ficus aurea). Fla Entomol. 1999, 82 (3): 454-461. 10.2307/3496871.View Article
- Pereira RAS, Prado AP: Non-pollinating wasps distort the sex ratio of pollinating fig wasps. Oikos. 2005, 110 (3): 613-619. 10.1111/j.0030-1299.2005.13234.x.View Article
- Yang C-Y, Wang R-W, Zhao G-F, Yang D-R: Diet of non pollinating wasps and their impact on the stability of fig-pollinator wasp mutualism. Zool Res. 2005, 26 (4): 379-385.
- Galil J, Dulberger R, Rosen D: The effects of Sycophaga sycomori L. on the structure and development of the syconia of Ficus sycomorus L. New Phytol. 1970, 69: 103-111. 10.1111/j.1469-8137.1970.tb04054.x.View Article
- Pereira RA, Teixeira SD, Kjellberg F: An inquiline fig wasp using seeds as a resource for small male production: a potential first step for the evolution of new feeding habits?. Biol J Linn Soc. 2007, 92 (1): 9-17. 10.1111/j.1095-8312.2007.00826.x.View Article
- Abdurahiman UC, Joseph KJ: Cleptoparasitism of the fig wasps (Torymidae: Chalcidoidea) in Ficus hispida L. Entomon. 1978, 3: 181-186.
- Gordh G, Headrick DH: A Dictionary of Entomology. 2001, New York. Oxon: CABI
- Ács Z, Challis R, Bihari P, Blaxter M, Hayward A, Melika G, Csóka G, Pénzes P, Pujade-Villar J, Nieves-Aldrey JL, et al: Phylogeny and DNA barcoding of inquiline oak gallwasps (Hymenoptera: Cynipidae) of the Western Palaearctic. Molecular Phylogenetics and Evolution. 2010
- Compton SV, van Noort S, Mcleish M, Deeble M, Stone V: Sneaky African fig wasps that oviposit through holes drilled by other species. African Natural History. 2009.
- Hamilton WD: Wingless and fighting males in fig wasps and other insects. Sexual selection and reproductive competition in insects. Edited by: Blum MS, Blum NA. 1979, London: Academic Press, 167-220.
- Ansari MH: The process of egg laying in Idarninae (Chalcidoidea: Hymenoptera). Indian J Entomol. 1967, 29: 380-384.
- Baker CF: A study of caprification in Ficus nota. Philippine Journal of Science (D). 1913, 8: 63-83.
- Bronstein JL: The non-pollinating wasp fauna of Ficus pertusa: exploitation of a mutualism?. Oikos. 1991, 61 (2): 175-186. 10.2307/3545335.View Article
- West SA, Herre EH, Windsor DM, Green RS: The ecology and evolution of the New World non-pollinating fig wasp communities. J Biogeogr. 1996, 23: 447-458. 10.1111/j.1365-2699.1996.tb00006.x.View Article
- Compton SG, McLaren FAC: Respiratory adaptations in some male fig wasps. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen (C). 1989, 92: 57-71.
- Ramirez WB: Breathing adaptations of males in fig gall flowers (Hymenoptera: Agaonidae). Rev Biol Trop. 1996, 44/45 (3/1): 277-282.
- Emlen DJ, Marangelo J, Ball JB, Cunningham CW: Diversity in the weapons of sexual selection: horn evolution in the beetle genus Onthophagus (Coleoptera: Scarabaeidae). Evolution. 2005, 59: 1060-1084.View ArticlePubMed
- Brisson JA: Aphid wing dimorphisms: linking environmental and genetic control of trait variation. Philosophical Transactions of the Royal Society B. 2010, 365: 605-616. 10.1098/rstb.2009.0255.View Article
- Matthews RW, Gonzalez JM, Matthews JR, Deyrup LD: Biology of the parasitoid Melittobia (Hymenoptera: Eulophidae). Annu Rev Entomol. 2009, 54: 251-266. 10.1146/annurev.ento.54.110807.090440.View ArticlePubMed
- Kjellberg F, Jousselin E, Bronstein JL, Patel A, Yokoyama J, Rasplus JY: Pollination mode in fig wasps: the predictive power of correlated traits. Proc R Soc Biol Sci Ser B. 2001, 268: 1113-1121. 10.1098/rspb.2001.1633.View Article
- Gibson GAP, Heraty JM, Woolley JB: Phylogenetics and classification of Chalcidoidea and Mymarommatoidea - a review of current concepts (Hymenoptera, Apocripta). Zool Scr. 1999, 28 (1-2): 87-124. 10.1046/j.1463-6409.1999.00016.x.View Article
- Geneious v3.7. [http://www.geneious.com/]
- Thompson JD, Higgins DG, Gibson JT: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignement through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.View ArticlePubMedPubMed Central
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.View ArticlePubMed
- Gillespie JJ, Johnston JS, Cannone JJ, Gutell RR: Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes of Apis mellifera (Insecta: Hymenoptera): structure, organization and retrotransposable elements. Insect Mol Biol. 2006, 15: 657-686. 10.1111/j.1365-2583.2006.00689.x.View ArticlePubMedPubMed Central
- Gillespie JJ, Munro JB, Heraty JM, Yoder MJ, Owen AK, Carmichael AE: A secondary structural model of the 28S rRNA expansion segments D2 and D3 for chalcidoid wasps (Hymenoptera: Chalcidoidea). Mol Biol Evol. 2005, 22 (7): 1593-1608. 10.1093/molbev/msi152.View ArticlePubMed
- Kjer KM: Use of rRNA secondary structure in phylogenetic studies to identify homologous positions: an example of alignment and data presentation from the frogs. Molecular Phylogenetics and Evolution. 1995, 4: 314-330. 10.1006/mpev.1995.1028.View ArticlePubMed
- Gillespie J, Cannone J, Gutell R, Cognato A: A secondary structural model of the 28S rRNA expansion segments D2 and D3 from rootworms and related leaf beetles (Coleoptera: Chrysomelidae; Galerucinae). Insect Mol Biol. 2004, 13 (5): 495-518. 10.1111/j.0962-1075.2004.00509.x.View ArticlePubMed
- Cruaud A, Jabbour-Zahab R, Genson G, Cruaud C, Couloux A, Kjellberg F, van Noort S, Rasplus J-Y: Laying the foundations for a new classification of Agaonidae (Hymenoptera: Chalcidoidea), a multilocus phylogenetic approach. Cladistics. 2010, 26 (4): 359-387.
- Aikake H: Information theory and an extension of the maximum likelihood principle. Second international symposium on information theory. Edited by: Petrov PN, Csaki F. 1973, Akad. Kiado, Budapest, 267-281.
- Nylander JAA: MrAIC.pl. Program distributed by the author. 2004, Upssala: Evolutionary Biology Centre, Uppsala University, 1.4.3
- Stamatakis A: Phylogenetic models of rate heterogeneity: A High Performance Computing Perspective. International Parallel and Distributed Processing Symposium (IPDPS 2006), Rhodes Island, Greece. 2006, 8-
- Maddison WP, Maddison DR: Mesquite: a modular system for evolutionary analysis. 2008, [http://mesquiteproject.org]
- Lewis PO: A likelihood approach to estimating phylogeny from discrete morphological character data. Syst Biol. 2001, 50: 913-925. 10.1080/106351501753462876.View ArticlePubMed
- Pagel M: The maximum likelihood approach to reconstructing ancestral character states of discrete characters on phylogenies. Syst Biol. 1999, 48: 612-622. 10.1080/106351599260184.View Article
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.