The ancient evolutionary origins of Scleractinia revealed by azooxanthellate corals
© Stolarski et al; licensee BioMed Central Ltd. 2011
Received: 22 June 2011
Accepted: 28 October 2011
Published: 28 October 2011
Scleractinian corals are currently a focus of major interest because of their ecological importance and the uncertain fate of coral reefs in the face of increasing anthropogenic pressure. Despite this, remarkably little is known about the evolutionary origins of corals. The Scleractinia suddenly appear in the fossil record about 240 Ma, but the range of morphological variation seen in these Middle Triassic fossils is comparable to that of modern scleractinians, implying much earlier origins that have so far remained elusive. A significant weakness in reconstruction(s) of early coral evolution is that deep-sea corals have been poorly represented in molecular phylogenetic analyses.
By adding new data from a large and representative range of deep-water species to existing molecular datasets and applying a relaxed molecular clock, we show that two exclusively deep-sea families, the Gardineriidae and Micrabaciidae, diverged prior to the Complexa/Robusta coral split around 425 Ma, thereby pushing the evolutionary origin of scleractinian corals deep into the Paleozoic.
The early divergence and distinctive morphologies of the extant gardineriid and micrabaciid corals suggest a link with Ordovician "scleractiniamorph" fossils that were previously assumed to represent extinct anthozoan skeletonized lineages. Therefore, scleractinian corals most likely evolved from Paleozoic soft-bodied ancestors. Modern shallow-water Scleractinia, which are dependent on symbionts, appear to have had several independent origins from solitary, non-symbiotic precursors. The Scleractinia have survived periods of massive climate change in the past, suggesting that as a lineage they may be less vulnerable to future changes than often assumed.
The two most popular hypotheses put forward to account for scleractinian origins are that they are either descendants of late Paleozoic rugose corals that survived the mass extinction at the Permian/Triassic boundary [1–3] or, that they evolved from soft-bodied (corallimorpharian-like) ancestors by gaining the ability to deposit a calcified skeleton [4–6]. Difficulties with the former hypothesis include that it requires major changes in both the composition of the skeleton, which was calcite in the case of Rugosa, but is aragonite in Scleractinia, and the symmetry of septal insertion , characters that are otherwise highly conserved. By contrast with Rugosa, some Permian fossils (known as scleractiniamorphs) appear to have had aragonite skeletons (Numidiaphyllum, Houchnagocyathus) and may be the immediate ancestors of some Triassic scleractinian coral lineages [7, 8]. Intriguingly, some early Paleozoic "scleractiniamorphs" (kilbuchophyllids from the Ordovician, ca. 450 Mya) have patterns of septal insertion that are indistinguishable from that of modern corals [9, 10], suggesting that these could represent the very early scleractinians. However, one objection to this idea has been the long time-gap separating the two groups in the fossil record.
Beyond implying that most extant scleractinians fall into two major clades (Robusta and Complexa) that are assumed to have diverged in the Late Carboniferous, ca. 300 Ma [11, 12], molecular data have so far not added significantly to our understanding of early coral evolution. One reason for this may be that molecular phylogenetics has focused primarily on shallow-water corals, most of which harbor symbiotic dinoflagellates commonly known as zooxanthellae, whereas azooxanthellate, deep-water corals that account for approximately half of extant scleractinian species, have largely been ignored in these analyses . The few studies that have included sequences from azooxanthellate scleractinians have led to conflicting interpretations of scleractinian phylogeny. For example, the phylogenetic reconstruction based on mitochondrial (12S rDNA) and nuclear (partial 28S rDNA) data for 80 scleractinian species (18 of which were azooxanthellate) suggested that all azooxanthellate, deep-water lineages originated from symbiotic, shallow-water ancestors . In contrast, another study based on COX1  found that members of the Gardineriidae and Micrabaciidae families formed a deeply diverging clade that may represent the oldest extant scleractinian lineage and that modern deep-water species diverge at or near the bases of both the Robusta and Complexa, implying that the evolutionary origin of scleractinians is best sought in deep-water rather than shallow-water (primarily zooxanthellate) coral species. These contradictory interpretations motivated us to extend phylogenetic analyses of a large and representative range of deep and shallow water corals (more than 10% of all extant deep-sea species; see Additional file 1) beyond COX1, to include data for the mitochondrial 12S and 16S rDNAs, and the nuclear 28S rDNA, in an attempt to clarify scleractinian origins and relationships. In addition, the ages of the major scleractinian lineages were estimated, and the origins of the Order explored. The divergence time estimates generated here bridge the gap with fossils, allowing the integration of the morphologically similar Paleozoic "scleractiniamorphs" into Scleractinia.
Results and Discussion
The discrepancy between the Complexa/Robusta divergence age estimated herein and those from previous studies may be due to a wider taxon sampling in the present study, and the quality  or absence of fossil calibration in the case of previous estimates [11, 12]. For example, the first estimate of the timing of divergence between Complexa/Robusta  was based on comparison of 16S rDNA sequence divergence with that in Orders of holometabolous insects, making no allowance for the possibility of different rates of evolution. Additionally, recent divergence time analysis of the Holometabola origin is placed in the early Carboniferous (355 Ma), significantly older than in previous reconstructions .
The divergence of unambiguous scleractinians (gardineriids and micrabaciids) deep in the Paleozoic removes the temporal disconnect between Scleractinia and "scleractiniamorphs", the only substantial basis on which the two groups were previously distinguished. The known Paleozoic "scleractiniamorphs" were solitary or quasi-colonial (phaceloid), which, under the evolutionary scenario outlined below, is consistent with the idea that the ancestral scleractinian was solitary and azooxanthellate. Based on the clear similarity between "scleractiniamorph" skeletons and extant scleractinians, we consider that the Paleozoic "scleractiniamorphs" [7–10] should be reclassified as genuine scleractinians. Moreover, other (soft-bodied) hexacorallian fossils have been reported from as far back as the Cambrian , and the results presented here lend support to the idea that these might represent evolutionary precursors of the Scleractinia . There are several possible explanations for the discontinuity of the Paleozoic record for Scleractinia. Paleozoic sediments containing corals may simply not yet have been found or are not preserved in the geological record. The only known lower Paleozoic scleractinian (genus Kilbuchophyllia) was recovered because shallow-water fossil-bearing deposits were transported to greater depth as the result of landslides . This indicates that the currently known Paleozoic record might not be representative of the true diversity of the group at that time. Alternatively, skeletal formation in these early corals might have been an ephemeral trait [5, 6], or skeleton-forming coral lineages went extinct. The same interpretative challenges apply to the evolutionary history of micrabaciids: their sudden appearance in the fossil record (Cretaceous) and lack of reliable ancestors among earlier scleractinian fauna suggest their emergence via skeletonization from an ancient "micrabaciid-gardineriid" skeleton-less hexacoral lineage, or points to huge gaps in the fossil record of deep-water scleractinians. Another important implication of the present analyses and those from the earlier COX1 analysis  is that modern shallow-water corals most likely had multiple independent origins from deep-water (azooxanthellate and solitary) ancestors (as has been hypothesized for another calcified cnidarian group, the Stylasteridae ), providing an explanation for the sudden appearance of the morphologically diverse Middle Triassic coral fauna.
Like their extant relatives, at least some Triassic Scleractinia hosted dinoflagellate symbionts - such associations conceivably evolved as a consequence of widespread oligotrophic conditions [24, 25]. The explosive diversification of scleractinians in the Middle Triassic (ca. 240 Ma) coincides with a massive radiation of dinoflagellates , the former presumably being facilitated by the establishment of symbiosis.
The analyses presented here support scleractinian monophyly and place the evolutionary origin of the Order deep in the Paleozoic, both of which are consistent with an independent origin from a soft-bodied ancestor but inconsistent with the rugosan ancestry hypothesis [1–3]. Although skeletal evidence is still lacking, the molecular data presented here bridge the gap in the fossil record between the Ordovician and Mesozoic Scleractinia. Although our results are robust and largely consistent with molecular-clock based analyses of other groups , the molecular markers used here may not be optimal for addressing deep-divergence events and should be verified using a range of additional markers.
The early origin of Scleractinia implied by our results has important implications for the debate about the fate of corals in times of global climate change, since they imply that the scleractinian lineage has persisted through several episodes of dramatic climate change during the last 450 My. Whilst on evolutionary time scales the Scleractinia may be less vulnerable than is sometimes assumed, the short-term survival of coral reefs as we know them is far less assured.
The present study was based on the examination of 123 lots of deep-water azooxanthellate scleractinians collected from 87 stations from New Caledonia (French research expeditions Bathus 3, Bathus 4, Halipro 1, Norfolk 1 and Norfolk 2), and from Australia (Australian research expeditions SS 011997, SS 102005, SS 022007, and Tan0308). Additional specimens collected in Australian waters were provided by the Western Australian Museum (see Additional file 1).
DNA preparation, amplification and sequence analyses
16S rDNA - the primers developed by Le Goff-Vitry et al.  (LP16SF 5' -TTGACCGGTATGAATGGTGT and LP16SR 5' -TCCCCAGGGTAACTTTTATC) were used to amplify a fragment whose size varied between 280 and 420 bp. Reactions were carried out in a total volume of 50 μl, and contained 0.2 mM dNTPs, 1.5 mM MgCl2, 1 mM of each primer, 1.5 units of Taq polymerase (Fisher Biotec - Australia) and 125 ng of template. The PCR protocol used was: an initial denaturation step (95°C for 5 min), then 35 cycles of 30 s at 94°C, 30 s at 50°C, and 45 s at 72°C, followed by 10 min at 72°C.
COX1 - the universal primers developed by Folmer et al.  (LCO1 490 5' -GGTCAACAAATCATAAAGATATTGG and HCO2 198 5' -TAAACTTCAGGGTGACCAAAAAATCA) were used to amplify a fragment whose size varied between 690 and 710 bp. Reactions were carried out as described by Folmer et al. : 95°C for 1 min, then 35 cycles of 30 s at 95°C, 30 s at 40°C, and 90 s at 72°C, followed by 10 min at 72°C.
12S rDNA - the primers developed by Chen and Yu  (ANTMT12SF 5'-AGCCACACTTTCACTGAAACAAGG and ANTMT12SR 5'-GTTCCCYYWCYCTYACYATGTTACGAC) were used to amplify a fragment whose size varied between 800 and 920 bp. Reactions were carried out in 50 μl, with 0.2 mM dNTPs, 1.5 mM MgCl2, 1 mM of each primer, 1.5 units of Taq polymerase (Fisher Biotec - Australia), and 125 ng of template. PCR conditions were: 95°C for 4 min, followed by 4 cycles of 30 s at 94°C, 60 s at 50°C, 120 s at 72°C, and 30 cycles of 30 s at 94°C, 60 s at 55°C, 120 s at 72°C and then 4 min at 72°C.
28S rDNA - the primers developed by Medina et al.  (28S.F63sq 5'-AATAAGCGGAGGAAAAGAAAC and 28S.R635sq 5'-GGTCCGTGTTTCAAGACGG) were used to amplify a fragment of approximately 750 bp. Reactions were carried out using the Advantage2 PCR kit (Clontech) with 100 ng of template, and following manufacturer's protocol. PCR conditions were: 95°C for 5 min, then 30 cycles of 30 s at 94°C, 60 s at 54°C, 90 s at 72°C, followed by 5 min at 72°C.
When amplification reactions based on Taq polymerase did not yield product, amplification was carried out using the Clontech Advantage-2 Kit (with the same template and primer concentrations, and under the same PCR protocol). PCR reactions were performed using a Bio-Rad DNA engine (Peltier Thermal Cycler). PCR products were purified using Mo-Bio Ultra Clean (PCR Clean Up) spin columns, and subjected to direct (Sanger) sequencing at Macrogen (South Korea).
Two different approaches were tested using sequences determined here and others retrieved from GenBank (see Additional file 1). The first approach included representatives of all hexacorallian orders but Ceriantharia and was intended to validate the Scleractinia monophyly. For this purpose, four single gene phylogenies all rooted with Octocorallia were constructed. The second approach, used herein for time divergence between scleractinian groups (Basal, Complex, and Robust groups), was based on concatenated sequences of the ribosomal genes16S rDNA and 28S rDNA, and included a broad range of scleractinian representatives. Alignments for both approaches were performed for each gene separately using ClustalW (EBI) and manually edited using JalView version 8.0 .
Alignments for the first approach were individually tested for substitution saturation  using DAMBE , which indicated little saturation for COX1 and 28S rDNA sequences (i.e. Iss. significantly lower than Iss.c), but higher levels of saturation for the 16S and 12S rDNAs (Iss. higher than Iss.c). Saturation related to mitochondrial ribosomal genes was induced by their respective fast evolving regions. This phenomenon was particularly evident because sequences from distant Anthozoa representatives were included in these alignments. To improve the phylogenetic signal, the most rapidly evolving regions were excluded from the alignment, resulting in a sharply decrease in saturation levels. The final alignments used in the first approach consisted of 298 positions for the16S rDNA, 599 positions for COX1, 631 positions for 12S rDNA, and 709 positions for the 28S rDNA. For each marker, appropriate models of nucleotide substitution were determined by the hierarchical likelihood ratio test implemented in MrModeltest . Phylogenetic analyses were performed using PhyML  for maximum likelihood (ML) and MrBayes (version 3.1.2)  for Bayesian Inference (BI). The maximum likelihood analyses were performed under the GTR model with a non-parametric Shimodaira-Hasegawa-like procedure. For the Bayesian inference, two runs each of 10 million generations were calculated for each marker with topologies saved at each 1000 generations, with the average standard deviation of split frequencies between runs of each marker converging to or less than 0.01. The first quarter of the 10000 saved topologies were discarded as burnin, and the remaining used to calculate posterior probabilities (Figure 1).
The final alignment that based the second approach contained concatenated 16S rDNA and 28S rDNA sequences (without excluding the fast evolving regions) from 121 scleractinians and 1 corallimorpharian, totalling 1334 bp. This alignment was also tested for substitution saturation, which indicated good phylogenetic signal. ML phylogenetic analyses were performed as described above. However, instead of Shimodaira-Hasegawa-like statistical support, they were performed under the Chi-square and 100 bootstrap replicates.
To estimate divergence times for gardineriids/micrabaciids and other scleractinians, we applied a relaxed-clock (uncorrelated lognormal) Bayesian Markov chain Monte Carlo method as implemented in BEAST (version 1.4.8) . This method allows nucleotide substitution rates to vary between lineages and incorporates phylogenetic uncertainty by sampling phylogenies and parameter estimates in proportion to their posterior probability. Additionally, Yule process was chosen as tree prior, and the prior distribution of divergence of each calibrated node was set as normal with standard deviation of 3.5. Hierarchical likelihood ratio tests led to the adoption of the General Time Reversible model with a proportion of invariant sites and gamma distributed rate heterogeneity (GTR+I+Γ) as the most appropriate evolutionary model for the molecular clock analyses. One run of 10 million generations was calculated with topologies and other parameters saved at each 1000 generations. A quarter of the 10000 saved topologies were discarded as burnin, and the remaining used to calculate posterior probabilities and node ages (Figure 1). Additionally, phylogenetic reconstruction from the same alignment was also calculated on MrBayes in two MCMC runs of 10 million generations each with topologies sampled every 1000 generations. Average standard deviation of split frequencies between runs was less than 0.01. The first quarter of the 10000 sampled topologies were discarded as burnin, and the remaining used to calculate posterior probabilities. The resulting topology was consistent with the one calculated using BEAST (data not shown).
For the calibration of the molecular clock, stringent constraints were applied based on fossils that can be unambiguously assigned to extant clades and whose unique skeletal characters can be unequivocally recognized in fossil coralla. Nodes used for the calibration were: (A) the appearance of Caryophyllia (ca. 160 Ma), based on the Late Jurassic (Oxfordian) species C. simplex and C. suevica [40, 41]. Both species have well-developed "true" pali present in one crown before the penultimate cycle of septa, fascicular columella composed of several twisted laths and septothecal walls, characters which together occur only in fossil and extant representatives of this genus ; (B) The divergence of the Dendrophylliidae (ca. 127 Ma), corresponding to the first occurrence of solitary Palaeopsammia (Barremian) . The first appearance of colonial dendrophylliids (Blastozopsammia) in the Albian (ca. 100 Ma)  is consistent with the earlier origin of solitary genera. Skeletal synapomorphies of dendrophylliids include the Pourtalès plan of septal arrangement and the presence of a synapticulothecate wall ; and (C) the origin of Flabellum (ca. 77.5 Ma), based on the earliest known record of the genus (F. fresnoense) from the Late Cretaceous (Coniacian; ?early Maastrichtian, based on foraminiferal assemblage) . Unequivocal Flabellum fossils are also known from the Late Cretaceous (?Campanian, Maastrichtian) of Seymour Island (F. anderssoni)  and Late Cretaceous (Maastrichtian) of Western Australia (Flabellum miriaensis) . Flabellum is clearly distinguishable based on the following unique combination of characters: the marginothecal wall is present throughout ontogeny, lack of pali/paliform lobes and scale-like microtexture of septa (sometimes preserved in the fossil record). Monophyly was enforced in the case of the dendrophylliids (calibration node B), but not for nodes A or C.
Ethanol preserved specimens were immersed in 20% (w/v) EDTA (pH 8) for two weeks at 4°C for decalcification. The resulting material was then dehydrated (70%, 80%, 2 × 95%, and 3 × 100% ethanol washes each of 40 min) and taken through three xylene washes (each of 40 min) prior to embedding in paraffin and serial tissue sectioning. Sections (5 μm) were stained using Harris's haematoxylin and eosin or Alcian Blue/PAS.
Skeleton preparation and analysis
Preliminary selection of skeleton samples was performed using a Nikon SMZ800 stereoscopic zoom microscope. For standard SEM (Philips XL 20) measurements, polished and etched blocks of corals skeleton were used. Following published methods of preparation , the samples were polished with diamond powder, 1200 Grit and aluminium oxide (Buehler TOPOL) and then etched for 10 seconds in 0.1% formic acid. Trace element analyses were performed with the Cameca NanoSIMS N50 at the Muséum National d'Histoire Naturelle (Paris), following established procedures [50, 51]. Briefly, septa were cut perpendicular to their growth direction, mounted in epoxy (Körapox©) and polished to a 0.25 μm finish using diamond paste. The samples were then gold-coated. Using a primary beam of O-, secondary ions of 24Mg+, 44Ca+ and 88Sr+ were sputtered from the sample surface and detected simultaneously (multicollection-mode) in electron-multipliers at a mass-resolving power of ~5000 (M/ΔM). At this mass-resolving power, the measured secondary ions are resolved from potential interferences. Data were obtained from a pre-sputtered surface as point analyses with the primary ions focused to a spot-size of ~3 micrometer and the primary beam stepped across the sample surface in steps of 20 micrometers. The measured 24Mg/44Ca and 88Sr/44Ca ratios were calibrated against analyses of carbonate standards of known composition (OKA-C) . The chemical variations recorded in the coral skeletons are much larger than both the internal and external reproducibility of the standards, which were less than < 5% for Mg/Ca and < 3% for Sr/Ca. Briefly, the composition of skeletal trace elements of micrabaciids and gardineriids are consistent with other modern deep-sea corals , although the Sr/Ca ratios measured from Letepsammia are at the high end of the range: Letepsammia - Mg/Ca = 1-2 mmol/mol, Sr/Ca = 11.5-12.2 mmol/mol; Stephanophyllia - Mg/Ca = 2.5-3 mmol/mol, Sr/Ca = 10.3-11.2 mmol/mol; and Gardineria - Mg/Ca = 1.7-3.5 mmol/mol, Sr/Ca = 9.3-11.7 mmol/mol.
This work was supported in part by the Polish Ministry of Science and Higher Education (project N307-015733) to JS, the European Research Council through Advanced Grant 246749 (BIOCARB) to AM, the Australian Research Council (ARC) via the ARC Centre of Excellence for Coral Reef Studies (ARC CoE) to DJM, and PhD scholarship support for MVK via Coordination for the Improvement of Higher Level Personnel (CAPES). The authors also thank Nancy Knowlton, Eldon Ball and Sylvain Forêt for their valuable comments on drafts of the manuscript. We would also like to acknowledge all the Smithsonian Institution invertebrate collection staff (NMNH) for sending the New Caledonian material to Australia, especially Paul Greenhall. Special thanks are due to all Museum of Tropical Queensland staff (MTQ-Queensland), especially Carden Wallace, Barbara Done for use of facilities at the Museum of Tropical Queensland. We also thank Philippe Bouchet (MNHN) who generously loaned the New Caledonian material from the Paris Museum to the Smithsonian Institution, and Bertrand Richer de Forges and IRD- Nouméa staff and collaborators for their great effort in collecting and preserving the New Caledonian specimens examined in the present study. We extend our gratitude to Felicity McEnnulty (CSIRO) for the loan the Australian specimens examined in the present study, which were collected with funding from the Fisheries Research and Development Corporation (FRDC), Environment Australia (now the Australian Government Department of Environment, Water, Heritage and the Arts), and by the Australian CSIRO (Commonwealth Scientific and Industrial Research Organization) Wealth from Oceans Flagship with the assistance of the Australian Government Department of the Environment, Water, Heritage and the Arts.
- Schindewolf O: Zur Kenntnis der Polycoelien und Plerophyllen. Eine Studie über den Bau der "Tetrakorallen" und ihre Beziehungen zu den Madreporarien. Abh Reichsamts Bodenforschung NF. 1942, 204: 1-324.Google Scholar
- Cuif JP: Arguments pour une relation phylétique entre les madréporaires paléozoïques et ceux du Trias. Implications systématiques de l'analyse microstructurale des Madréporaires triasiques. Mém S Geo F. 1977, 129: 1-54.Google Scholar
- Stolarski J: Gardineria - scleractinian "living fossil". Acta Palaeontol Pol. 1996, 41: 339-367.Google Scholar
- Oliver WA: Origins and relationships of Paleozoic coral groups and the origin of the Scleractinia. Paleont Soc Papers. 1996, 1: 107-134.Google Scholar
- Stanley GD, Fautin DG: The origins of modern corals. Science. 2001, 291: 1913-1914. 10.1126/science.1056632.View ArticlePubMedGoogle Scholar
- Stanley GD: The evolution of modern corals and their early history. Earth-Sci Rev. 2003, 60: 195-225. 10.1016/S0012-8252(02)00104-6.View ArticleGoogle Scholar
- Ezaki Y: The Permian coral Numidiaphyllum: new insights into anthozoan phylogeny and Triassic scleractinian origins. Palaeontology. 1997, 40: 1-14.Google Scholar
- Ezaki Y: Palaeoecological and phylogenetic implications of a new scleractiniamorph genus from Permian sponge reefs, south China. Palaeontology. 2000, 43: 199-217. 10.1111/1475-4983.00124.View ArticleGoogle Scholar
- Scrutton CT, Clarkson ENK: A new scleractinian-like coral from the Ordovician of the Southern Uplands, Scotland. Palaeontology. 1991, 34: 179-194.Google Scholar
- Scrutton CT: A new kilbuchophyllid coral from the Ordovician of the Southern Uplands, Scotland. Cour Forsch Senck. 1993, 164: 153-158.Google Scholar
- Romano SL, Cairns SD: Molecular phylogenetic hypotheses for the evolution of scleractinian corals. B Mar Sci. 2000, 67: 1043-1068.Google Scholar
- Romano SL, Palumbi SR: Evolution of scleractinian corals inferred from molecular systematics. Science. 1996, 271: 640-642. 10.1126/science.271.5249.640.View ArticleGoogle Scholar
- Cairns SD: Deep-water corals: an overview with special reference to diversity and distribution of deep-water scleractinian corals. B Mar Sci. 2007, 81: 311-322.Google Scholar
- Barbeitos MS, Romano SL, Lasker HR: Repeated loss of coloniality and symbiosis in scleractinian corals. Proc Natl Acad Sci USA. 2010, 107: 11877-10.1073/pnas.0914380107.View ArticlePubMedPubMed CentralGoogle Scholar
- Kitahara MV, Cairns SD, Stolarski J, Blair D, Miller DJ: A comprehensive phylogenetic analysis of the Scleractinia (Cnidaria, Anthozoa) based on mitochondrial CO1 sequence data. PLoS ONE. 2010, 5: e11490-10.1371/journal.pone.0011490.View ArticlePubMedPubMed CentralGoogle Scholar
- Fukami H, Chen CA, Budd AF, Collins A, Wallace C, Chuang YY, Chen C, Dai CF, Iwao K, Sheppard C, Knowlton N: Mitochondrial and nuclear genes suggest that stony corals are monophyletic but most families of stony corals are not (Order Scleractinia, Class Anthozoa, Phylum Cnidaria). PLoS ONE. 2008, 3: e3222-10.1371/journal.pone.0003222.View ArticlePubMedPubMed CentralGoogle Scholar
- Medina M, Collins AG, Takaoka TL, Kuehl JV, Boore JL: Naked corals: Skeleton loss in Scleractinia. Proc Natl Acad Sci USA. 2006, 103: 9096-9100. 10.1073/pnas.0602444103.View ArticlePubMedPubMed CentralGoogle Scholar
- Sperling EA, Robinson JM, Pisani D, Peterson KJ: Where's the glass? Biomarkers, molecular clocks, and microRNAs suggest a 200-Myr missing Precambrian fossil record of siliceous sponges. Geobiology. 2010, 8: 24-36. 10.1111/j.1472-4669.2009.00225.x.View ArticlePubMedGoogle Scholar
- Wiegmann BM, Trautwein MD, Kim J, Bertone M, Winterton SL, Cassel BK, Yeates DK: Single-copy nuclear genes resolve the phylogeny of the holometabolous insects. BMC Biology. 2009, 7: 34-10.1186/1741-7007-7-34.View ArticlePubMedPubMed CentralGoogle Scholar
- Hou XG, Stanley GD, Zhao J, Ma XY: Cambrian anemones with preserved soft tissue from the Chengjiang biota, China. Lethaia. 2005, 38: 193-203. 10.1080/00241160510013295.View ArticleGoogle Scholar
- Ezaki Y: Paleozoic Scleractinia: progenitors or extinct experiments?. Palaeobiology. 1998, 24: 227-234.Google Scholar
- Clarkson ENK, Harper DAT, Owen AW, Taylor CM: Ordovician faunas in mass-flow deposits, Southern Scotland. Terra Nova. 2007, 4: 245-253.View ArticleGoogle Scholar
- Lindner A, Cairns SD, Cunningham CW: From offshore to onshore: Multiple origins of shallow-water corals from deep-sea ancestors. PLoS ONE. 2008, 3 (6): e2429-10.1371/journal.pone.0002429.View ArticlePubMedPubMed CentralGoogle Scholar
- Riedel P: Korallen in der Trias der Tethys: Stratigraphische Reichweiten, Diversitätsmuster, Entwicklungstrends und Bedeutung als Rifforganismen. Mitt Geol Bergbaustud Oesterr. 1991, 37: 97-118.Google Scholar
- Stanley G, van de Schootbrugge B: The Evolution of the Coral-Algal Symbiosis. Coral Bleaching. Edited by: van Oppen MJH, Lough JM. 2009, Berlin: Springer, 7-19.View ArticleGoogle Scholar
- MacRae RA, Fensoma RA, Williams GL: Fossil dinoflagellate diversity, origins and extinctions and their evolutionary significance. Can J Bot. 1996, 74: 1987-1694.View ArticleGoogle Scholar
- Wells JW: Scleractinia. Treatise on Invertebrate Paleontology, part F, Coelenterata. Edited by: Moore RC. 1956, Lawrence: The University of Kansas Press, F328-F444.Google Scholar
- Janiszewska K, Stolarski J, Benzerara K, Meibom A, Mazur M, Kitahara M, Cairns SD: A unique skeletal microstructure of the deep-sea micrabaciid scleractinian corals. J Morphol. 2011, 272: 191-203. 10.1002/jmor.10906.View ArticlePubMedGoogle Scholar
- Vaughan TW, Wells JW: Revision of the suborders, families, and genera of the Scleractinia. Spec Pap Geol Soc Am. 1943, 44: 1-363.Google Scholar
- Le Goff-Vitry MC, Rogers AD, Baglow D: A deep-sea slant on the molecular phylogeny of the Scleractinia. Mol Phyloge Evol. 2004, 30: 167-177. 10.1016/S1055-7903(03)00162-3.View ArticleGoogle Scholar
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R: DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994, 3: 294-297.PubMedGoogle Scholar
- Chen CA, Yu JK: Universal primers for amplification of mitochondrial subunit ribosomal RNA-encoding gene in scleractinian corals. Mar Biotech. 2000, 2: 146-153.View ArticleGoogle Scholar
- Clamp M, Cuff J, Searle SM, Barton GJ: The Jalview Java alignment editor. Bioinf. 2004, 20: 426-427. 10.1093/bioinformatics/btg430.View ArticleGoogle Scholar
- Xia XH, Xie Z, Salemi M, Chen L, Wang Y: An index of substitution saturation and its application. Mol Phylog Evol. 2003, 26: 1-7. 10.1016/S1055-7903(02)00326-3.View ArticleGoogle Scholar
- Xia XH, Xie Z: DAMBE: Data analysis in molecular biology and evolution. J Hered. 2001, 92: 371-373. 10.1093/jhered/92.4.371.View ArticlePubMedGoogle Scholar
- Nylander JAA: MrModeltest 2.0. Evolutionary Biology Centre, Uppsala University., [http://darwin.uvigo.es/software/jmodeltest.html]
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology. 2003, 52: 696-704. 10.1080/10635150390235520.View ArticlePubMedGoogle Scholar
- Huelsenbeck JP, Ronquist F: MrBAYES: Bayesian inference of phylogenetic trees. Bioinf. 2001, 17: 754-755. 10.1093/bioinformatics/17.8.754.View ArticleGoogle Scholar
- Drummond A, Rambaut A: BEAST is a cross-platform program for Bayesian MCMC analysis of molecular sequences. [http://beast.bio.ed.ac.uk/Main_Page]
- Geyer OF: Die oberjurassische Korallenfauna von Wiirttemberg. Palaeontographica A. 1954, 104: 121-220.Google Scholar
- Lauxmann U: Revision der oberjurassischen Korallen von Württemberg (SW -Deutschland), excl. Fungiina. Palaeontografica A. 1991, 219: 107-175.Google Scholar
- Kitahara MV, Cairns SD, Miller DJ: Monophyletic origin of Caryophyllia (Scleractinia, Caryophylliidae), with descriptions of six new species. Syst Biodivers. 2010, 8: 91-118. 10.1080/14772000903571088.View ArticleGoogle Scholar
- Wanner J: Die Fauna der obersten weissen Kreide der libyschen Wüste. Palaeontographica. 1902, 30: 91-151.Google Scholar
- Filkorn HF, Pantoja AJ: A new Early Cretaceous coral (Anthozoa; Scleractinia; Dendrophylliina) and its evolutionary significance. J Paleontol. 2004, 78: 501-512. 10.1666/0022-3360(2004)078<0501:ANECCD>2.0.CO;2.View ArticleGoogle Scholar
- Cairns SD: A generic revision and phylogenetic analysis of the Dendrophylliidae (Cnidaria: Scleractinia). Smithson Contrib Zool. 2001, 615: 1-75.View ArticleGoogle Scholar
- Durham JW: Corals from the Gulf of California and the North Pacific coast of America. Geol Soc Am Mem. 1947, 20: 1-68.View ArticleGoogle Scholar
- Felix J: Über die fossilen Korallen der Snow Hill-Insel und der Seymour-Insel: Wissenschaftliche Ergebnisse der Schwedischen Südpolar-Expedition 1901-1903. 1909, 3: 1-15.Google Scholar
- Jell JS, Cook AG, Jell PA: Australian Cretaceous Cnidaria and Porifera. Alcheringa. 2011, 35: 241-284.Google Scholar
- Stolarski J: 3-Dimensional micro- and nanostructural characteristics of the scleractinian corals skeleton: A biocalcification proxy. Acta Palaeontol Pol. 48: 497-530.
- Meibom A, Cuif JP, Hillion F, Constantz BR, Juillet-Leclerc A, Dauphin Y, Watanabe T, Dunbar RB: Distribution of magnesium in coral skeleton. Geophys Res Lett. 2004, 31: L23306-Google Scholar
- Meibom A, Mostefaoui S, Cuif JP, Dauphin Y, Houlbreque F, Dunbar R, Constantz BR: Biological forcing controls the chemistry of reef-building coral skeleton. Geophys Res Lett. 2007, 34: L02601-Google Scholar
- Bice KL, Layne GD, Dahl K: Application of secondary ion mass spectrometry to the determination of Mg/Ca in rare, delicate, or altered planktonic foraminifera: Examples from the Holocene, Paleogene, and Cretaceous. Geochem Geophys Geosyst. 2005, 6: Q12P07-Google Scholar
- Meibom A, Cuif JP, Mostefaoui S, Dauphin Y, Houlbreque F, Meibom K, Dunbar R: Compositional variations at ultra-structure length-scales in coral skeleton. Geochim Cosmochim Acta. 2008, 72: 1555-1569. 10.1016/j.gca.2008.01.009.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.