Female house sparrows "count on" male genes: experimental evidence for MHC-dependent mate preference in birds
© Griggio et al; licensee BioMed Central Ltd. 2011
Received: 17 September 2010
Accepted: 14 February 2011
Published: 14 February 2011
Females can potentially assess the quality of potential mates using their secondary sexual traits, and obtain "good genes" that increase offspring fitness. Another potential indirect benefit from mating preferences is genetic compatibility, which does not require extravagant or viability indicator traits. Several studies with mammals and fish indicate that the genes of the major histocompatibility complex (MHC) influence olfactory cues and mating preferences, and such preferences confer genetic benefits to offspring. We investigated whether individual MHC diversity (class I) influences mating preferences in house sparrows (Passer domesticus).
Overall, we found no evidence that females preferred males with high individual MHC diversity. Yet, when we considered individual MHC allelic diversity of the females, we found that females with a low number of alleles were most attracted to males carrying a high number of MHC alleles, which might reflect a mating-up preference by allele counting.
This is the first experimental evidence for MHC-dependent mating preferences in an avian species to our knowledge. Our findings raise questions about the underlying mechanisms through which birds discriminate individual MHC diversity among conspecifics, and they suggest a novel mechanism through which mating preferences might promote the evolution of MHC polymorphisms and generate positive selection for duplicated MHC loci.
Darwin suggested that female choice can help explain the evolution of extravagant secondary sexual characters in males, but he struggled over how to understand why females evolve mating preferences for such males . Jerram Brown decided to "put aside the idea that there is a best male and that he is best for every female," and instead, he argued that females should prefer genetically compatible or heterozygous males to increase offspring heterozygosity or genetic diversity  (also see [3–5]). He was inspired by studies on house mice (Mus musculus) that found disassortative mating preferences for genes of the major histocompatibility complex (MHC) [6, 7]. MHC genes are a multigene family in vertebrates that encode cell-surface glycoproteins (class I and II molecules) that control antigen presentation to T-lymphocytes, and through this mechanism MHC genes play a pivotal role in immune recognition of pathogens and parasites. MHC-disassortative mating preferences may function to increase offspring heterozygosity - MHC or genome-wide - as both can enhance resistance to infectious diseases [8–12]. Furthermore, MHC-disassortative mating preferences can also help to explain the extraordinary polymorphism of MHC genes . More recent studies have found MHC-dependent mating preferences in fish [14–17], reptiles , and primates and other mammals [19–21]. However; more studies are needed, especially in birds and other wild, outbred species [22–25]. Our aim was to test whether (and how) MHC genes influence mating preferences in house sparrows (Passer domesticus).
Several observational studies suggest that MHC genes play a role in mate choice in birds. First, a study on pheasants (Phasianus colchicus) suggests that females prefer males with "superior" disease-resistant MHC-genotypes, as predicted by good genes models of sexual selection . Second, a study in Savannah sparrows (Passerculus sandwichensis) found evidence that females avoid males sharing similar MHC alleles . Third, a study on house sparrows found evidence that females avoid mating with males that have low individual MHC diversity and males that are too dissimilar (no common alleles) at MHC (class I) loci . Fourth, a study on Seychelles warblers (Acrocephalus sechellensis) found that females were more likely to have extra-pair offspring when their social mate had low MHC diversity, and the MHC diversity of the extra-pair male was higher than that of the cuckolded male . Finally, MHC genes may also play a role in cryptic mate choice, as suggested in studies on fish, birds and mammals [21, 30–33]. For example, it has recently been found that in peacocks (Pavo cristatus), females lay more eggs when mated with males with high individual MHC diversity . Moreover, in red jungle fowl (Gallus gallus), males invest less sperm when copulating with females carrying similar MHC alleles . Taken together, observational studies in the wild and experimental studies on cryptic mate preferences provide intriguing evidence that MHC genes influence mating preferences in birds.
MHC-dependent mating preferences might function to enhance offspring heterozygosity, or produce offspring with intermediate or optimal levels of MHC-heterozygosity . The "optimal heterozygosity" hypothesis follows from models suggesting that expressing more MHC molecules during thymic selection has negative effects on the development of the T cell repertoire . Interestingly, this hypothesis is directly supported by studies on stickleback fish (Gasterosteus aculeatus): individuals vary in the number of MHC alleles they carry (due to variation in heterozygosity, number of loci, or both), and females with a low number of MHC alleles prefer males with a high individual diversity, whereas females with high diversity prefer males with low individual diversity ("allele optimization strategy") . Thus, in sticklebacks, females' preferences are based on the number rather than the similarity of alleles they share with prospective mates , and this preference is functional because individuals with an intermediate number of MHC alleles are the most resistant to parasites [35, 36]. Unlike disassortative mating, however, it is unclear how such sexual selection for optimizing offspring heterozygosity can explain or contribute to the evolution of MHC polymorphisms . Therefore, it is still unclear whether MHC-dependent mating preferences provide a general explanation for the evolution of MHC polymorphisms, or not.
Generalized linear mixed model investigating variation in female mate preferences.
Male wing length
Male tarsus length
Male body mass
Male badge size
Stimulus individual heterozygosity
Female group × Stimulus group
Female group × Stimulus individual heterozygosity
Female group × Male badge size
GLM post hoc test (Tukey honestly significant difference test) for the effect of the interaction between female group and stimulus group on female mate preference (see Table 1).
We found no significant evidence that females prefer males carrying a particular number of MHC alleles, as predicted by the good genes hypothesis . However, we found that females discriminate among males carrying different levels of individual MHC allelic diversity, and females' preferences depend upon their own and the individual diversity of potential mates. More specifically, females with a low number of alleles spent significantly more time near males carrying a high number of alleles, which might reflect a "mating up" tactic. Unlike stickleback fish , however, females with intermediate or high number of alleles did not show any significant preference based on males' MHC diversity. To our knowledge, our results provide the first experimental evidence in birds that MHC genes play a role in mating preferences. It is unclear whether our findings predict actual mating patterns in the wild, although they are consistent with an observational study on a wild population of house sparrows that found evidence that females avoid mating with males with low individual MHC diversity .
There are several reasons to suspect that this mating-up preference by allele counting might enhance offspring disease resistance and fitness. First, a previous study on house sparrows found that mating pairs with high individual MHC diversity had offspring with high individual diversity , which suggests that females with low diversity can increase individual (and brood) diversity of their offspring by mating up. Second, another study with house sparrows found MHC-dependent immune responses (assayed with phytohemagglutinin and sheep red blood cells) , and although the number of individual MHC alleles had no detectable effect, a study on peacocks found greater immune responses to phytohemagglutinin with increased individual MHC diversity . Third, an experimental study with stickleback fish indicates that there is an optimal number of individual MHC alleles for mounting immune defenses against multiple parasites , which means that females with low individual MHC diversity should increase offspring disease resistance by mating with males carrying high diversity. Nevertheless, further studies are needed to determine the expression of MHC in sparrows and to understand how MHC allele number affects host immune resistance.
Moreover, our findings are consistent with recent studies indicating that females' quality or condition influences their mating preferences [39–42]. For example, female house mice show odour preferences for outbred over inbred males, though only inbred females show this preference . House sparrows, in fact, provide another example of such condition-dependent preferences . The black throat patch (badge) of the males is an intensively studied plumage trait that appears to be involved in female mate choice, though differences exist among populations. A recent study found that females in poor body condition, unlike those in good condition, preferred males with average-size badges. Taken together, our results here are consistent with the idea that females' mating preferences vary depending upon their own quality.
After dividing females according to their individual number of MHC alleles, we found that females with a low number of alleles are most attracted to males carrying a high number of MHC alleles, which might reflect a mating-up preference by allele counting. Our findings raise questions about the phenotypic cues sparrows utilize to assess MHC diversity among conspecifics and the evolutionary consequences of these preferences. We found no evidence that individual MHC diversity was associated with any phenotypic trait (body size or size of ornaments) (see also ). It has been widely assumed that birds are microsmatic or anosmatic; however, there is increasing behavioral, physiological as well as genetic evidence that their olfactory abilities are better than generally assumed (reviewed in [22, 45]), raising the possibility that some birds might utilize olfactory cues to assess potential mates. Indeed, T-maze experiments have found that crested auklets (Aethia cristatella) exhibited an attraction to conspecific feather odour and preferentially orientated towards two chemical components of feather scent . Using a similar apparatus, it was demonstrated that blue petrels (Pachyptila desolata), could discriminate between their own, their mate's and an unknown conspecific's odour, and were attracted to their mate's odour . Moreover, it was found that the volatile compounds in the preen oil (preen gland secretions) of a songbird, the dark-eyed junco (Junco hyemalis), contain reliable information about individual identity, sex and population of origin . Thus, it is plausible that MHC-dependent mate choice in birds is mediated by olfactory mechanisms. It remains to be seen whether mating with males having high individual MHC diversity provides indirect benefits for low diversity females. Similarly, it has been suggested that homozygous females have the most to gain by mating with heterozygous males (for a review see ). Finally, our findings suggest that mating preferences can potentially provide a selective factor favoring MHC allelic diversity in populations, duplication of MHC loci and copy number variation. Duplications that increase the number of MHC loci must eventually have negative consequences on individual immunity, but the selective forces shaping the number and diversity of MHC loci within a species may include mating preferences tracking individual immunological optima, which likely varies in time and space.
Subjects and housing
Males and female house sparrow were collected at the Vienna Zoo (47°56'N, 16°45'E), Cobenzl (48°16'N, 16°19'E) and Feuersbruun (48°26'N, 15°47'E) in the winter preceding the experiment. A total of 54 focal females and 156 stimulus males and 39 stimulus females were housed outdoors in seventeen aviaries, in which they were attributed at random (aviary size: 3.5 m × 3.5 m × 3 m; about fifteen individuals per aviary). All birds were over 1 year old. All aviaries were equipped in the same way with vegetation, several perches (about seven per aviary). Commercial food for granivorous passerines and water were provided ad libitum. The initiation of breeding immediately after the experiment and several successful breeding attempts suggest that the housing conditions and experiment were appropriate and had no negative effect on the birds' health or condition.
MHC characterization (DNA isolation, PCR and SSCP)
Genomic DNA was extracted from blood samples with a DNA extraction kit (DNeasy Blood and Tissue Kit, QIAGEN GmbH) according to the manufacturer's protocol. We estimated the overall number of MHC alleles per individual by amplifying exon 3 of a class I locus, which corresponds to the peptide-binding region (PBR) [50–52]. PCR amplifications were performed using a fluorescent (6'-FAM) labelled primer (23 M- GCG CTC CAG CTC CTT CTG CCC ATA) and an unlabeled primer (A21M- GTA CAG CGG CTT GTT GGC TGT GA) [50, 51]. The PCR amplification (T1 thermocycler, Biometra) contained a final volume of 25 μL, which included 50 to 100 ng of genomic DNA, 0.6 μM of each primer and 12.5 μL Multiplex PCR Kit (QIAGEN GmbH) (containing hot-start DNA polymerase, PCR buffer and dNTP mix). The PCR program began with 15 min initial heating at 95°C followed by 35 cycles of 30 s denaturation at 94°C, 35 s annealing at 64°C and 90 s extension at 72°C. A final elongation step was run at 72°C for 10 min. To control for PCR artifacts, we used a high-end polymerase and 2 step negative controls (for both the PCR and capillary sequencer).
MHC diversity was screened using capillary electrophoresis single strand conformation polymorphism (CE-SSCP) [50, 51, 53]. The fluorescent-labelled PCR samples were prepared for electrophoresis by combining 1 μL PCR product with 14 μL loading mix (13.5 μL Hi-DI formamide, 0.5 μL of in-house prepared ROX size standard, ). The mixture was heated for 3 min at 95°C to separate the complementary DNA strands, chilled on ice for 4 min and analysed by capillary electrophoresis (ABI PRISM 3130 xl automated DNA Sequencer, Applied Biosystems). The CE-SSCP polymer consisted of 5% GeneScan polymer (Applied Biosystems), 10% glycerol, 1xTBE, and HPLC-water. The running buffer mixture contained 10% glycerol, 1xTBE and HPLC-water. The separation of the allelic variants was achieved by run conditions at 12 kV for 36 min and by a run temperature at 24°C. The retention times of the allelic variants were identified relative to the ROX size standard. GeneMapper software (version 4.05 Applied Biosystems) was used to process the SSCP data. Peak pattern results were reproducible as they were run 3 × with size standards.
Microsatellite typing and heterozygosity
Heterozygosity was assessed using six microsatellite markers: Pdo3, Pdo5, Pdo6, Pdo8, Mcyu4 and Ase18 ( and references therein). Single microsatellite marker amplifications were run in a T1 thermocycler (Biometra) in a final volume of 12.5 μL including 50 to 100 ng of genomic DNA, 5 pmol of the forward and the reverse primer, 1U DNA polymerase (FirePol), 3 mM MgCl2, 100 μM dNTPs, and 1× PCR Buffer. After an initial denaturation at 95°C for 5 min, 35 amplification cycles were performed with denaturation at 94°C for 30 s, annealing at 58°C for 90 s, and extension at 72°C for 90 s. A final elongation step was conducted at 72°C for 10 min. The fluorescent-label single microsatellite markers were pooled and fragment analysis was performed (Beckman Coulter CEQ8000 automated sequencer). Number of alleles and mean observed heterozygosity for each locus were, respectively, Pdo3: 20 alleles, 0.87; Pdo5: 25 alleles, 0.74; Pdo6: 82 alleles, 0.91; Pdo8: 18 alleles, 0.34; Mcyu4: 30 alleles, 0.80; and Ase18: 22 alleles, 0.91 (consistent with ).
Mate preference experiment
We thank Christa Grabmayer and Wolfgang Pegler who coordinated the care of the house sparrows, as well as Anna Grasse and Hanja Brandl for help with performing microsatellite genotyping. We thank Helmut Schaschl for initially adapting CE-SSCP to MHC genotyping in sparrows for another project. We thank Gopi K. Munimanda for his help with ROX size standard optimization and CE- SSCP. We thank Alessandro Devigili and Simone Pirrello for their help to analyze the videos. We thank Tim Birkhead, Scott Edwards, Jan L. Lifjeld, David Richardson, Gabriele Sorci and an anonymous referee for their constructive and helpful comments on previous versions of the manuscript. This work was funded by the Austrian Science foundation FWF (grant no: P19130-B17 to HH). All of the manipulations of birds performed during this study comply with the current laws of the country in which they were performed.
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