Population structure of Bactrocera dorsalis s.s., B. papayae and B. philippinensis (Diptera: Tephritidae) in southeast Asia: evidence for a single species hypothesis using mitochondrial DNA and wing-shape data
- Mark K Schutze†1, 2Email author,
- Matthew N Krosch†1, 2,
- Karen F Armstrong1, 3,
- Toni A Chapman1, 4,
- Anna Englezou1, 4,
- Anastasija Chomič3,
- Stephen L Cameron2,
- Deborah Hailstones1, 4 and
- Anthony R Clarke1, 2
© Schutze et al.; licensee BioMed Central Ltd. 2012
Received: 5 April 2012
Accepted: 19 July 2012
Published: 30 July 2012
Bactrocera dorsalis s.s. is a pestiferous tephritid fruit fly distributed from Pakistan to the Pacific, with the Thai/Malay peninsula its southern limit. Sister pest taxa, B. papayae and B. philippinensis, occur in the southeast Asian archipelago and the Philippines, respectively. The relationship among these species is unclear due to their high molecular and morphological similarity. This study analysed population structure of these three species within a southeast Asian biogeographical context to assess potential dispersal patterns and the validity of their current taxonomic status.
Geometric morphometric results generated from 15 landmarks for wings of 169 flies revealed significant differences in wing shape between almost all sites following canonical variate analysis. For the combined data set there was a greater isolation-by-distance (IBD) effect under a ‘non-Euclidean’ scenario which used geographical distances within a biogeographical ‘Sundaland context’ (r 2 = 0.772, P < 0.0001) as compared to a ‘Euclidean’ scenario for which direct geographic distances between sample sites was used (r 2 = 0.217, P < 0.01). COI sequence data were obtained for 156 individuals and yielded 83 unique haplotypes with no correlation to current taxonomic designations via a minimum spanning network. beast analysis provided a root age and location of 540kya in northern Thailand, with migration of B. dorsalis s.l. into Malaysia 470kya and Sumatra 270kya. Two migration events into the Philippines are inferred. Sequence data revealed a weak but significant IBD effect under the ‘non-Euclidean’ scenario (r 2 = 0.110, P < 0.05), with no historical migration evident between Taiwan and the Philippines. Results are consistent with those expected at the intra-specific level.
Bactrocera dorsalis s.s., B. papayae and B. philippinensis likely represent one species structured around the South China Sea, having migrated from northern Thailand into the southeast Asian archipelago and across into the Philippines. No migration is apparent between the Philippines and Taiwan. This information has implications for quarantine, trade and pest management.
KeywordsGeometric morphometrics Cytochrome c oxidase I South China Sea biogeography Fruit flies
Population studies on the B. dorsalis species complex have focused on B. dorsalis s.s. within that species’ described geographic distribution, predominantly northern southeast Asia and southern China, west to Bangladesh and east to the Pacific [16–21]. Current theory based on genetic data places the origin of B. dorsalis s.s. either in southern-central China , or the southeast corner of mainland China . Genetically diverse populations in southern China are as distinct from each other as from those in southeast Asia (i.e., Myanmar, Laos, and Vietnam) and are believed to be structured by mountain ranges and air currents, rather than purely by geographic distance [17, 18, 21]. Dispersal of B. dorsalis s.s. individuals is generally limited to within 50 km of their origin [23–25]; however longer distance fly movements (presumably wind assisted) of 100 – 250 km have been reported in southern China [19, 26]. A combined analysis of population structure of B. dorsalis s.s. with its closely related sibling species, B. papayae and B. philippinensis, in the biogeographically complex South China Sea area has never been undertaken. This is primarily because the three taxa are regarded as distinct species, each occupying different distributions within the region: B. dorsalis s.s. around the northern edge (north of the Thai/Malay peninsula), B. papayae around the western and southern edge (Thai/Malay peninsula and into the Indonesian archipelago) and B. philippinensis at the eastern edge (the Philippines and Borneo) (Figure 1). However if no a priori assumptions are made that these taxa represent separate species then a population-level analysis using samples obtained throughout this region may contribute towards resolving species boundaries. And given the geographical complexity of southeast Asia around the South China Sea, such an analysis should be undertaken in the context of the region’s history.
The region of southeast Asia surrounding the South China Sea has experienced considerable tectonic activity , repeated sea level fluctuations  and associated climatic and vegetation changes [29, 30]. Numerous cycles of sea level changes over the last 250,000 years , including several periods during which sea levels fell to 120 m below present, resulted in repeated connections between today’s mainland southeast Asia and the major islands of Sumatra, Java, and Borneo via the Thai-Malay peninsula. Such sea level drops exposed the vast Sunda Shelf and formed the region known as ‘Sundaland’ [28, 31]. During periods when Sundaland was exposed to its maximum area (the size of Europe, see Figure 1), the landmass was vegetated by an essentially open savannah corridor interspersed by forest refugia . This allowed increased migration of fauna, including forest-dependant species, throughout the present island chains of the region . However as sea levels rose, the Sunda Shelf re-submerged and land-bridges – such as the Thai-Malay Isthmus of Kra – narrowed. Furthermore, closed forest habitats throughout the region were periodically reduced to isolated refugia in response to cooling climates . These events enforced dispersal barriers, restricting movement from mainland southeast Asia into the peninsula and archipelago for many species of invertebrates, reptiles, birds and mammals [32–37]. Given this complex history, a unified biogeographic pattern for all local taxa is unlikely , and any biogeographic study within this region must be examined on its own merits. Regarding B. dorsalis s.l., for example, we may predict that the historical movement of flies throughout this region may have been facilitated during the exposure of Sundaland, and subsequently restricted as sea levels once again rose to potentially cut off dispersal routes.
We have identified the need to study the genetic and morphological variation of B. dorsalis s.l. in an area which had not previously been investigated, i.e., extending southwards of mainland China and including the region surrounding the South China Sea. In light of the possibility that B. dorsalis s.s. B. papayae and B. philippinensis represent the same biological species (further supported by the authors’ unpublished work demonstrating mating compatibility), we approached this study with no a priori assumptions based on current taxonomic designations. If these three species represent one biological entity, we predict to observe active gene flow among the groups alongside results consistent with prior population genetic studies depicting a south Chinese origin; the latter being in line with expectations based on a biogeographical history of migration southwards from China into the Indonesian archipelago and across to the Philippines. Alternatively, if the current taxonomy of the three species is correct, we predict very different results. These include tighter correlations between taxonomic species designations and haplotype distributional relationships, consistent and significant differences in pair-wise measures of genetic or morphometric distances between taxonomic species but not for populations within them, and poor isolation by distance effects across the breadth of the sampled geographic range. Furthermore, we have explicitly chosen to exclude the closely related species B. carambolae Drew & Hancock from comprehensive analysis presented here. While this species is closely allied to B. dorsalis s.s. B. papayae and B. philippinensis[8, 9], a comprehensive survey of B. carambolae across its native and invasive ranges has revealed it to i) be reciprocally monophyletic (via a muli-locus phylogenetic analysis; authors’ unpublished data); ii) relatively sexually incompatible (authors’ unpublished data); and iii) not share any COI haplotypes with the latter three species (see Methods and Additional file 1); thereby confirming its specific status as separate from B. dorsalis s.s. B. papayae and B. philippinensis and warranting its exclusion from the population-level analyses presented here.
For this study, the cytochrome c oxidase subunit 1 (COI) mitochondrial DNA (mtDNA) gene was selected for analysis as it is a relatively fast evolving and practical locus from which to derive recent evolutionary genetic histories. The COI gene has also been successfully applied previously to assess population structure for B. dorsalis s.s.[17–20] and for a diverse variety of other insects [39–44]. Although we are aware of the caveats regarding the use and interpretation of mtDNA in studies of historical population structure [45, 46], especially where the focus is the species and not the organelle , its usage in conjunction with other methods is still generally considered a valid contribution towards assessing species divergence and historical population characteristics. In addition to mtDNA analysis, we therefore apply geometric morphometric shape analysis of wings as an independent measure of population structure for B. dorsalis s.s. B. papayae and B. philippinensis. Geometric morphometric analysis quantifies shape variation among individuals or defined groups [48, 49], and such information has demonstrated effectiveness for discriminating groups at both inter- and intraspecific levels across a range of taxa [50–53], including members of the B. dorsalis species complex . While the use of geometric morphometric shape analysis in studies of population structure continues to grow, its direct use with genetic data from the same individuals as an independent and parallel dataset remains uncommon despite its documented potential [51, 55, 56].
This study therefore uses two independent data sets (mtDNA and wing shape) to assess geographic variation for flies sampled across much of the distributional range of B. dorsalis s.s., B. papayae, and B. philippinensis in order to assess whether such variation aligns with that expected at the intra- or interspecific level. We further aimed to determine if the historical migration patterns hypothesised from our data concur with what is known of the regions’ rich geographical history.
Collection and sample size data for B. dorsalis s.l. in this study
Sample size (genetics/shape)
Number of COI haplotypes
Taipei, China (Taiwan)
B. dorsalis s.s.
San Pa Tong, Thailand
B. dorsalis s.s.
B. dorsalis s.s.
Nakhon Si Thammarat, Thailand
B. dorsalis s.s.
Quezon City, Philippines
A priori groups were significantly different (P < 0.05) from each other based on permutation tests for Mahalanobis distances for all comparisons except that between San Pa Tong (north Thailand) and Bangkok (central Thailand) (P = 0.41) (see Additional file 1: Table S2). Comparisons of Procrustes distances yielded fewer significant differences among sample sites. Sites located on the Thai/Malay peninsula and Sumatra (i.e. San Pa Tong, Bangkok, Nakhon Si Thammarat, Penang, Serdang, and Lampung) were not significantly different from each other with respect to Procrustes distances, whereas Taiwan and both Philippine sites (Quezon City and Imus) were significantly different for all pairwise comparisons, including the notable difference between Quezon City and Imus despite their geographic proximity (see Additional file 1: Table S2).
Sequence data for the mtDNA gene COI yielded 83 unique haplotypes from 156 individuals from nine sites across southeast Asia (Table 1). The ratio of transitions to transversions was high at 9.108, which could be indicative of sequence saturation or recent divergence, among other scenarios . Measures of genetic diversity within sites suggested that populations were quite diverse (see Additional file 1: Table S3). The population parameter θπ ranged from 0.118 ± 0.218 (Quezon City) to 4.632 ± 2.635 (Serdang) and gene diversity ranged from 0.118 ± 0.101 (Quezon City) to 0.994 ± 0.019 (Bangkok). Indeed, all but three sites (Quezon City, Imus and Lampung) possessed gene diversities greater than 0.9. Tajima’s D tests of neutrality for the total dataset were negative and statistically significant (D = −2.265, P < 0.0001), suggesting either that the sequences may be under selection or that populations may have experienced relatively recent historical expansion.
Estimates of pairwise Φ ST indicate distinct population differentiation among almost all sites, except among sites within the described geographic range of B. dorsalis s.s. (Taiwan, San Pa Tong, Bangkok and Nakhon Si Thammarat); pairwise comparisons among these four sites were non-significant (see Additional file 1: Table S4). In contrast, sites within the described ranges of B. papayae and B. philippinensis all differed significantly to each other, despite some being geographically proximate (as for the geometric morphometric shape analysis, e.g., Quezon City and Imus).
Regression of genetic distance (pairwise Φ ST ) against geographic distance revealed a weak but significant positive association for both Euclidean (Figure 3e) and ‘non-Euclidean’ geographic distance comparisons (Figure 3f). However, unlike the geometric morphometric results, the regression of genetic distance against Euclidean geographic distance was slightly stronger (Pearson correlation = 0.360; r2 = 0.129; P < 0.05) as compared with the ‘non-Euclidean’ comparison (Pearson correlation = 0.331; r2 = 0.110; P < 0.05).
Tests of population expansion using Fu’s F S were negative and statistically significant across the entire dataset (F S = −7.133, P = 0.019). Individually, six of the nine sites possessed significant estimates of F S (see Additional file 1: Table S3). The mismatch distribution for the total dataset was unimodal (r = 0.0328, R 2 = 0.0219 – see Additional file 1: Figure 2a), suggesting a strong signature of historical population expansion. This was supported by the Bayesian GMRF Skyride plot (see Additional file 1: Figure 2b), which suggested gradual expansion of populations since the mid- to late-Pleistocene (300 - 600kya).
The pattern of spatial structure resolved for both genetic and geometric morphometric datasets does not correlate with the current taxonomic designations of B. dorsalis s.s., B. papayae and B. philippinensis. Rather, it is consistent with the hypothesis that these three taxa represent a single species that is widely distributed throughout southeast Asia. Further, our data infer that B. dorsalis s.l. has undergone several periods of range expansion throughout its history in southeast Asia. The genetic and geometric morphometric datasets are broadly congruent regarding the biogeographical history of the taxon, albeit with some differences. Based on our sampled range, B. dorsalis s.l. originated in northern Thailand and has undergone a gradual range expansion southward along the Thai/Malay peninsula. There followed migration into Sumatra and eastward to the Philippines, although whether that occurred sequentially or concurrently remains unclear. We recovered no evidence of historical movement between the relatively geographically close (~350 km apart) islands of Taiwan and the Philippines.
By broadening the sample range across wider geographic distributions, it becomes possible to re-evaluate relationships within groups of organisms that contain taxa for whose biological identities are unresolved. Such a scenario has recently been highlighted in the southeast Asian region for Anopheles mosquitoes, whereby a Philippine species (A. limosus King) was redefined as a population of the widespread southeast Asian species, A. vagus Dönitz . Similarly, biogeographical population-level studies of B. dorsalis s.l. have until now been limited to southern China and northern Asia, the presumed Asian range of B. dorsalis s.s.. However, the incorporation of samples of B. papayae and B. philippinensis with B. dorsalis s.s. without a priori species boundary assumptions allows for a more extensive biogeographical analysis throughout southeast Asia.
Based on the two independent datasets presented here (shape and mtDNA) there is strong evidence that B. dorsalis s.l. had a northern southeast Asian origin, a finding congruent with previous studies [16, 22], and that it has subsequently dispersed further southwards into southeast Asia. The movement of flies from northern Thailand appears to have commenced some 540 thousand years ago, reaching the modern Philippines approximately 360 thousand years ago and Sumatra 265 thousand years ago. The COI data suggests the flies may have dispersed in multiple directions, with the correlation of genetic distance against Euclidian distance slighter stronger than the correlation of genetic difference against ‘non-Euclidian’ distance (Figure 3e & f). While there is insufficient difference between these tests to support one hypothesis over the other, the estimated arrival of flies into the Philippines approximately 100,000 years before they arrived in Sumatra (Figure 6) implies that there was not a simple, unidirectional dispersal down Thailand, across Indonesia and up into the Philippines. It may be that flies from (current) central Thailand simultaneously moved both eastward and southward across Sundaland, with the eventual Philippine populations tracking along the eastern (inside) edge of Sundaland (see Figure 1 for Sundaland geography), independent of the populations spreading south into Malaysia and the Indonesian Archipelago. In stark contrast, it is difficult to interpret the wing shape analysis in any way except as a continuous, unidirectional anti-clockwise spread around the South China Sea (Figure 3d). We must emphasise that while results here indicate movement of flies between Thailand and Taiwan began approximately 416kya (Figure 6), this is almost certainly an analytical artifact resulting from our lack of intermittent sample sites in southern mainland China and neighbouring regions (e.g. Vietnam).
While it is possible that the conclusions provided by one methodology are more accurate than the other (i.e. COI versus shape data), we believe the two data sets reveal different aspects of the same story. The COI data is resolving older historical gene flow, whereas the limited evidence available (see below) suggests that the shape data may be detecting subtle differences resulting from gene flow in more recent history. Thus it may be that initial colonisation of southeast Asia by B. dorsalis s.l. involved dispersal in multiple directions across the exposed Sundaland shelf, while contemporary fly movement is restricted to the current land-arc surrounding the South China Sea, resulting in the extraordinarily strong relationship seen between wing shape and ‘non-Euclidian’ geographic distance. Wing shape variation for these species (and also B. carambolae) has previously been examined using historical collection material, with the aim of determining if shape variation could be used as a species discriminatory character . While results from that study demonstrated differences, species from that study were a priori defined prior to analysis (unlike here) and, by the nature of the discriminate analysis used, strongly biased results into finding differences. Despite this, a high degree of similarity among taxa (including between B. dorsalis s.s. and B. papayae) was still identified and it was argued that such variation may be more indicative of that at the intra- rather than inter-specific level, with additional information (such as presented here) required to resolve the true biological relationships as ‘wing shape information is not, in isolation, an argument to confirm or refute species limits’ . There are few examples where both geometric morphometric shape data and genetic information have been applied simultaneously to address questions of population structure. In the absence of direct genetic tests, the ability to detect wing shape differences in individuals of the same species subjected to different larval environments , or from different sample sites , is indirect evidence of the potential of shape data to detect subtle, more recent changes. Other researchers have gone some way towards comparing between shape and genetic data, such as in a study of cranial shape variation in South American Ctenomys rodents, which was compared with previously published mtDNA and microsatellite information . While neither genetic nor morphometric data revealed strong population structuring, cranial shape data was nevertheless shown to be as sensitive as molecular data . Less common are direct comparisons between shape and molecular information using the same individuals, however an analysis of wing shape variation versus microsatellite data of Glossina palpalis gambiensis Vanderplank individuals collected from Burkina Faso, Africa, represents an impressive example of the power of such studies . In this instance the geometric morphometric shape data detected population structuring not evident in the genetic analysis. As COI (used in our study) is not suited to contemporary gene flow as were the microsatellites used for the G. palpalis gambiensis study , we recommend the future application of microsatellite data for B. dorsalis s.l. studies. Regardless of the outcome of future work, however, the differences in the biogeographic stories told by the independent data sets in this paper reinforces the value of using multiple tools in studies of biogeographically complex regions.
We are unable to explain the relatively large divergence in both wing shape and haplotype variation between the two Philippine sites of Quezon City and Imus. Our analysis of mtDNA implies the occurrence of two historical migration events from Thailand, however these two locations are only 24 km apart and we have no reason to believe that the flies used here represent two distinct populations. We suspect that the observed difference may be driven by a haplotype that is shared between Imus, San Pa Tong and Penang. This haplotype is located centrally in the network, several mutational steps from the other more common cluster of Philippine haplotypes and is connected to other haplotypes sampled from mainland sites, along with a further singleton from Imus. Whilst we cannot be sure, we argue that this pattern may be representative of two separate migration events into the Philippines, and the starburst-like pattern of haplotype relationships that characterises the two clusters of Philippine haplotypes, along with low genetic diversity at these two sites, appears to support this scenario. Nevertheless, there are also some haplotypes shared between Quezon City and Imus and the results of the analyses may simply be the consequence of low sample size, especially for Imus (n = 13). Such uncertainty is exacerbated by the relatively large difference in wing shape between the two B. philippinensis samples which may represent some form of secondary contact between them and B. dorsalis s.s. or B. papayae, and while we have not explicitly considered human-mediated movement as a factor influencing observed patterns, it cannot be discounted and also warrants further attention. We therefore recommend this area be re-sampled before asserting hypotheses regarding multiple migrations events into the Philippines. Despite this however, B. philippinensis is extremely closely related to B. dorsalis s.s. and B. papayae, and while specific dispersal pathways remain unclear we believe the patterns observed concur with those expected under an intra-specific hypothesis for these three taxa.
The treatment of these three taxa as a single biological species poses considerable taxonomic implications. While extensive past efforts have been directed toward identifying consistent diagnostic markers for B. dorsalis s.s., B. papayae and B. philippinensis, this result is yet to be achieved. The question therefore remains: do such diagnostic markers exist but we are looking in the wrong places? Or rather has the original taxonomy been incorrect inasmuch as B. papayae and B. philippinensis should not be erected to species status but rather considered as populations of B. dorsalis s.s.? In light of our results, we believe the latter more likely. If B. papayae and B. philippinensis are treated as conspecific with B. dorsalis s.s. then searching for diagnostic markers to resolve among these ‘species’ is no longer necessary; with the only diagnostic requirement being the discrimination of B. dorsalis s.s. from other closely related B. dorsalis species complex flies for which morphological or molecular markers often already exist (such as for B. carambolae). Notwithstanding this, we recognise the necessity to treat our current study as one line of evidence towards what must be part of a broader integrative taxonomic resolution.
The three currently defined species of B. dorsalis s.s. B. papayae and B. philippinensis display genetic and morphometric variation congruent with the hypothesis that they represent the same biological species. Moreover, our data supports a northern southeast Asian origin for B. dorsalis s.l. (in accordance with previous studies), with dispersal directed southwards and eastwards into the Malay archipelago and the islands of the Philippines over the last 500,000 years. This historical movement was likely facilitated during periods of lower sea level and the exposure of the vast Sundaland shelf, but gene flow has since been more restricted between mainland southeast Asia and the island chains, possibly due to sea level rises forming geographic barriers, such as that hypothesised for the Isthmus of Kra on the Thai/Malay peninsula and the current seaways between the islands of the Indonesian archipelago. As only the second study to concurrently use genetic and geometric morphometric data, but seeing the same synergies between the approaches as recorded in the first study , we support the further use of independent, fine scale morphological information (such as shape analysis) in parallel with genetic data as a means of more completely assessing population structure for biogeographical and related studies. Finally, a reappraisal of the taxonomic relationships among these highly pestiferous tephritid species poses considerable implications for basic research, pest management, quarantine practices and international trade. In recognising the limitations of this study, particularly the potential for further sampling and the use of other independent markers, we endorse the need for further studies across other disciplines (e.g. behavioural and cytogenetic) to further clarify the biological relationships among these taxa before any formal taxonomic changes are made.
Study sites & sample collection
Adult male flies were collected from nine sites across southeast Asia; one site in China (Taiwan), three in Thailand, two in peninsular Malaysia, one in Indonesia and two in the Philippines (Figure 1; Table 1). Samples of B. dorsalis s.s., B. papayae and B. philippinensis were collected between May 2009 and December 2010. Flies were collected using methyl eugenol/insecticide baited hanging traps containing propylene glycol as a preserving agent for all sites except Serdang Malaysia. As lures only attract males, females were not incorporated in the analysis. Samples from Serdang (taxonomically confirmed as B. papayae by R.A.I. Drew) were reared from Musa acuminata x balbisiana hybrids, vars. Mas, Berangan and Lemak bananas collected in November 2010. All samples other than those collected from Serdang were shipped to Queensland University of Technology (QUT), Brisbane Australia, for morphological identification (MKS) and processing; Serdang flies were reared from infested fruit at the UN/FAO International Atomic Energy Agency Agriculture and Biotechnology Laboratories, Seibersdorf Austria. Sample sizes from each site were unknown until the completion of sampling and logistical constraints prevented revisiting sample sites. Flies were identified based on descriptions in Drew & Hancock (1994). Three legs were removed (fore, mid and hind) for genetic analysis and one wing (usually the right) for geometric morphometric shape analysis. There was not a complete 1:1 correlation between material used for genetic and shape analysis due to difficulties in amplifying the COI gene for some wing samples and some flies used for genetic analysis had damaged wings. Nevertheless over 90% of the material examined here had both COI sequence data and wing shape data successfully used. Voucher samples are held at QUT.
Geometric morphometric analyses
The size of each wing was assessed as ‘centroid size’, an isometric estimator of size calculated as the square root of the summed distances of each landmark from the centre of the landmark configuration; and was calculated using the computer program MORPHOLOGIKA v2.5 . One-way analysis of variance followed by the Tukey post hoc test was applied to a priori groups based on sample location in order to determine significant differences (α = 0.05) among sites with respect to wing size.
Raw landmark coordinate data were imported into the computer program MORPHOJ v1.02E  for shape analysis. Data were first subjected to Procrustes superimposition to remove all but shape variation . Multivariate regression of the dependant wing-shape variable against centroid size (independent variable) was conducted to assess the effect of wing size on wing shape (i.e. allometry) [54, 61]. The statistical significance of this regression was tested by permutation tests (10,000 replicates) against the null hypothesis of independence. To correct for allometric contribution towards shape variation, subsequent analyses were undertaken using the residual components as determined from the regression of shape on centroid size.
Samples were a priori assigned to one of nine sample location groups (as for centroid size analysis), from which subsequent canonical variates analysis (CVA) was applied to determine relative differences in wing shape among groups. Significant differences were determined via permutation tests (1000 permutation rounds) for Mahalanobis distances among groups.
We regressed geographic distance (km) against Mahalanobis distances as calculated from CVA to test for ‘Isolation by Distance’ (IBD) effects . Geographic distance was calculated in one of two ways to test the hypothesis that population variation was structured around the South China Sea by: 1) Euclidean geographic distance and 2) ‘non-Euclidean’ geographic distance. Euclidean distances represented the shortest possible geographic distance between pairs of sample locations with no prior biogeographic assumptions; whereas ‘non-Euclidean’ distance was measured as the sum of all respective distances between sample sites extending through the peninsula, into the archipelago and up to the Philippines (see Figure 3 a & b). For example, the Euclidean distance between Taipei and Quezon City is 1,155 km (the shortest distance possible), whereas the ‘non Euclidean’ distance is the sum of distances between all sample sites from Taiwan, across to the mainland, down the peninsula, into the archipelago and up to the Philippines (7,586 km). The ‘non-Euclidean’ distance measure was used as it closely approximated geographic distances between sample sites for when sea levels were lower (i.e. when more of the ‘Sundaland’ land mass was exposed) and therefore represents our hypothetical pathway for historic land-restricted dispersal by B. dorsalis s.l. The strength of the association for either approach was determined by linear regression analysis using the program SPSS v17.0.
Leg material for genetic analysis was sent to the Elizabeth Macarthur Agricultural Institute (EMAI), New South Wales Australia, for DNA extraction. DNA was extracted from each fly using the Qiagen DNeasy Blood and Tissue kit according to the manufacturer’s instructions which was then subaliquoted into a master stock and stored at −20 °C as two working stock solutions. One working stock was sent to Lincoln University, Christchurch New Zealand, for sequencing of a 642 bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene.
Polymerase chain reactions were undertaken with forward FolA and reverse FolB primers  using either (1) the Expand High Fidelity (HiFi) PCR System (Roche Diagnostics GmbH, Mannheim, Germany) with 1 mM MgCl2, primers at 60 ng each, 0.2 mM dNTPs, 1× PCR buffer, 0.525 U enzyme mixture and 0.7 μL DNA in a total volume of 10 μL or (2) GoTaq® Green Master Mix (Promega, Madison, USA) with primers at 250 ng each and 0.5 μL of DNA template in a total volume of 20 μL. Thermocycling conditions were 94 °C for 2 min, then 40 cycles of 94 °C for 15 s, 50 °C for 30 s and 72 °C for 45 s, followed by 7 min at 72 °C. We used PCR boost (Biomatrica, San Diego, USA) as a substitute for water in cases where samples failed to produce enough PCR product for sequencing using Expand High Fidelity (HiFi) PCR System. All PCR products were checked in 1% agarose gels containing SYBR SafeTM DNA Gel Stain (Invitrogen, Carlsbad, USA) in 0.5× TBE buffer. Both directions of PCR products were sequenced at the Lincoln University Bio-Protection Research Centre, using primers FolA and FolB and ABI Big Dye (ABI, Foster City, USA) technology on ABI PRISM 3130xl Genetic Analyzer (ABI, Foster City, USA) according to the manufacturer’s recommendations. COI sequences are available under the GenBank Accession Numbers JX099580 - JX099755.
COI sequences were aligned using BioEdit Version 7.0.5  and checked by eye for discrepancies. Tests for sequence saturation were conducted by calculating the mean ratio of transitions to transversions in MEGA Version 4.0 . Tajima’s D tests of neutrality were estimated for the total dataset and for each individual population using 1000 coalescent simulations in Arlequin Version 3.11  to determine if sequences were evolving neutrally. Gene diversity and the population parameter, θπ, were calculated using Arlequin to estimate genetic diversity within sites. A haplotype network was constructed using the median-joining method followed by maximum parsimony post-processing in Network Version 220.127.116.11 . Supporting information for the exclusion of B. carambolae is presented as a haplotype network including B. carambolae which reveals it to share no haplotypes with the three target species (n = 20; 13 unique haplotypes; Additional file 1: Figure S3). These 20 specimens of B. carambolae were field collected from Serdang, Malaysia (native distribution) and Paramaribo, Suriname (invasive distribution).
Various methods for partitioning genetic variation within and among sites were implemented. Conventional among-site ΦST indices (P < 0.05) incorporating the Tamura-Nei model of evolution  were estimated in ARLEQUIN to explore the level of connectivity among sites. We used hierarchical analyses of molecular variance (AMOVA ) computed in ARLEQUIN using ΦST estimates to test hypotheses of a priori site groupings. Statistical significance for these methods was obtained through 1000 random permutations. Clustering of sites based on relative ΦST estimates was performed using multidimensional scaling in accordance with  and using the ALSCAL analysis in the PASW Statistics Version 18 software package (formerly SPSS). Populations are converted to points in a two-dimensional space, with the linear distances between points proportional to the relative ΦST estimates among populations. Similar to wing-shape data, hypotheses of IBD were assessed by linear regression analysis between geographical distance (Euclidean and ‘non-Euclidean’) and genetic distance among groups (ΦST).
We tested hypotheses of post-isolation population expansion by estimating Fu’s FS  for the total dataset and for each site individually in ARLEQUIN (obtaining statistical support via 1000 coalescent simulations) and by plotting the mismatch distribution of pairwise differences and their frequency in DnaSP Version 5.0 . We also plotted relative population size changes over time using Bayesian Gaussian Markov Random Field (GMRF) Skyride Plots  in BEAST Version 1.5.3 . Due to the lack of useful fossil calibrations for this analysis, we attempted to account for molecular rate uncertainty in two ways. First, we used an uncorrelated lognormal relaxed molecular clock model to allow rates to vary along branches. Second, we ran three sets of analyses using the fastest (2.28%/my – ) and slowest (0.9%/my – ) insect mitochondrial substitution rates, as well as a standard invertebrate mitochondrial divergence rate of 1.5%/my  as the rough median. We employed a Hasegawa-Kishino-Yano (HKY) substitution model and a time-aware prior on the smoothing of the scaled effective population size. Two runs of 30 million generations each were implemented for the three analyses and log and tree files were combined in LogCombiner Version 1.5.3  to produce GMRF Skyride Plots.
To investigate the geographical spread of haplotypes among the study sites through time we implemented the discrete phylogeographical analysis in BEAST . We used a Bayesian stochastic search variable selection (BSSVS) procedure and incorporated a relaxed lognormal molecular clock, Bayesian GMRF Skyride demographic model and HKY substitution model as described above. We ran three sets of analysis using the three molecular rates described above to provide an age range for inferred migration events. Two runs of 30 million generations each were performed for each analysis and the resulting geotree files were combined and annotated with location states in TREEANNOTATOR Version 1.5.3 . The annotated tree was then converted to a keyhole markup language (KML) file using SPREAD , a program that converts the output of discrete phylogeographic analysis from BEAST into a ‘kml file’ prior to visualisation in Google Earth. Convergence of runs – and thus support for the inferred ages of migration events – was achieved by ensuring estimated sample sizes (ESS) for the ‘geotreelikelihood’ prior were greater than 200 in the combined log file of each analysis.
We are sincerely thankful to Ju Chun Hsu, Ken Hong Tan, Richard Bull, Yuvarin Boontop, Wigunda Rattanapun, Sotero Resilva, Vijay Shanmugam and Hanifah Yahaya for assisting us with local field collections. We also thank R.A.I. Drew for assistance in identifying specimens. Filip Bielejec and Philippe Lemey provided invaluable assistance with the phylogeographic analysis of DNA sequence data in BEAST. The paper was produced with research support through CRC National Plant Biosecurity projects 20115 and 20183. The authors would like to acknowledge the support of the Australian Government’s Cooperative Research Centres Program and the New Zealand Tertiary Education Commission.
- White IM, Elson-Harris MM: Fruit flies of economic significance: their identification and bionomics. 1992, CAB International, Wallingford UK
- Clarke AR, Armstrong KF, Carmichael AE, Milne JR, Roderick GK, Yeates DK: Invasive phytophagous pests arising through a recent tropical evolutionary radiation : the Bactrocera dorsalis complex of fruit flies. Annu Rev Entomol. 2005, 50: 293-319. 10.1146/annurev.ento.50.071803.130428.PubMedView Article
- Fletcher BS: Life history strategies of Tephritid fruit flies. Fruit flies: their biology, natural enemies and control. Edited by: Robinson AS, Hooper G. 1989, Elsevier, New York, 195-208.
- Drew RAI, Hancock DL: The Bactrocera dorsalis complex of fruit flies (Diptera: Tephritidae: Dacinae) in Asia. Bull Entomol Res Suppl. 1994, 2 (i-iii): 1-68.View Article
- Iwahashi O: Aedeagal length of the Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), and its sympatric species in Thailand and the evolution of a longer and shorter aedeagus in the parapatric species of B. dorsalis. Appl Entomol Zool. 2001, 36 (3): 289-297. 10.1303/aez.2001.289.View Article
- Armstrong KF, Ball SL: DNA barcodes for biosecurity: invasive species identification. Phil Trans R Soc B. 2005, 360 (1462): 1813-1823. 10.1098/rstb.2005.1713.PubMedPubMed CentralView Article
- Yong HS: Genetic differentiation and relationships in five taxa of the Bactrocera dorsalis complex (Insecta: Diptera: Tephritidae). Bull Entomol Res. 1995, 85: 431-435. 10.1017/S0007485300036166.View Article
- Muraji M, Nakahara S: Phylogenetic relationships among fruit flies, Bactrocera (Diptera, Tephritidae), based on the mitochondrial rDNA sequences. Insect Mol Biol. 2001, 10 (6): 549-559. 10.1046/j.0962-1075.2001.00294.x.PubMedView Article
- Smith PT, Kambhampati S, Armstrong KA: Phylogenetic relationships among Bactrocera species (Diptera: Tephritidae) inferred from mitochondrial DNA sequences. Mol Phylogenet Evol. 2003, 26: 8-17. 10.1016/S1055-7903(02)00293-2.PubMedView Article
- Fletcher MT, Kitching W: Chemistry of fruit flies. Chem Rev. 1995, 95 (4): 789-828. 10.1021/cr00036a001.View Article
- Tan KH: Interbreeding and DNA analysis of sibling species within the Bactrocera dorsalis complex. Recent trends on sterile insect technique and area-wide integrated pest management - economic feasibility, control projects, farmer organization and Bactrocera dorsalis complex control study. 2003, 113-122.
- Medina FIS, Carillo PAV, Gregorio JS, Aguilar CP: The mating compatibility between Bactrocera philippinensis and Bactrocera dorsalis. Abstracts, 5th International Symposium on Fruit Flies of Economic Importance: 1–5 June. Edited by: Tan KH. 1998, Penang, Malaysia, 155-
- Sites-Jnr JW, Marshall JC: Delimiting species: a Renaissance issue in systematic biology. Trends Ecol Evol. 2003, 18: 462-470. 10.1016/S0169-5347(03)00184-8.View Article
- Edwards SV, Kingan SB, Calkins JD, Balakrishnan CN, Jennings WB, Swanson WJ, Sorenson MD: Speciation in birds: genes, geography, and sexual selection. Proc Natl Acad Sci USA. 2005, 102: 6550-6557. 10.1073/pnas.0501846102.PubMedPubMed CentralView Article
- Fitzpatrick BM, Fordyce JA, Gavrilets S: Pattern, process and geographic modes of speciation. J Evolution Biol. 2009, 22: 2342-2347. 10.1111/j.1420-9101.2009.01833.x.View Article
- Aketarawong N, Bonizzoni M, Thanaphum S, Gomulski LM, Gasperi G, Malacrida AR, Gugliemino CR: Inferences on the population structure and colonization process of the invasive oriental fruit fly, Bactrocera dorsalis (Hendel). Mol Ecol. 2007, 16: 3522-3532. 10.1111/j.1365-294X.2007.03409.x.PubMedView Article
- Chen P, Ye H: Relationship among five populations of Bactrocera dorsalis based on mitochondrial DNA sequences in western Yunnan, China. J Appl Entomol. 2008, 132: 530-537. 10.1111/j.1439-0418.2008.01302.x.View Article
- Li Y, Wu Y, Chen H, Wu J, Li Z: Population structure and colonization of Bactrocera dorsalis (Diptera: Tephritidae) in China, inferred from mtDNA COI sequences. J Appl Entomol. 2012, 136: 241-251. 10.1111/j.1439-0418.2011.01636.x.View Article
- Liu J, Shi W, Ye H: Population genetics analysis of the origin of the Oriental fruit fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae), in northern Yunnan Province, China. Entomol Sci. 2007, 10: 11-19. 10.1111/j.1479-8298.2006.00194.x.View Article
- Shi W, Kerdelhue C, Ye H: Population genetics of the Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae), in Yunnan (China) based on mitochondrial DNA sequences. Environ Entomol. 2005, 34 (4): 977-983. 10.1603/0046-225X-34.4.977.View Article
- Vargas RI, Stark JD, Nishida T: Population dynamics, habitat preferences, and seasonal distribution patterns of Oriental Fruit Fly and Melon Fly (Diptera: Tephritidae) in an agricultural area. Environ Entomol. 1990, 19 (6): 1820-1828.View Article
- Wan X, Nardi F, Zhang B, Liu Y: The Oriental Fruit Fly, Bactrocera dorsalis, in China: origin and gradual inland range expansion associated with population growth. PLoS One. 2011, 6 (10): e25238-10.1371/journal.pone.0025238.PubMedPubMed CentralView Article
- Iwahashi O: Movement of the Oriental fruit fly adults among islets of the Ogasawara Islands. Environ Entomol. 1972, 1 (2): 176-179.View Article
- Froerer KM, Peck SL, McQuate GT, Vargas RI, Jang EB, McInnis DO: Long distance movement of Bactrocera dorsalis (Diptera: Tephritidae) in Puna, Hawaii: How far can they go?. Am Entomol. 2010, 56: 88-94.View Article
- Liang F, Wu JJ, Liang GQ: The first report of the test on the flight ability of oriental fruit fly. Acta Agric Univ Jiangxiensis. 2001, 23 (2): 259-260.
- Chen P, Ye H, Mu QA: Migration and dispersal of the oriental fruit fly, Bactrocera dorsalis, in regions of Nujiang River based on fluorescence mark. Acta Ecol Sin. 2007, 27 (6): 2468-2476.
- Hall R: Cenozoic geological and plate tectonic evolution of SE Asia and the SW Pacific: computer-based reconstructions, models and animations. J Asian Earth Sci. 2002, 20: 353-431. 10.1016/S1367-9120(01)00069-4.View Article
- Voris HK: Maps of Pleistocene sea levels in Southeast Asia: shorelines, river systems and time durations. J Biogeogr. 2000, 27: 1153-1167. 10.1046/j.1365-2699.2000.00489.x.View Article
- Bird MI, Taylor D, Hunt C: Palaeoenvironments of insular Southeast Asia during the Last Glacial Period: a savanna corridor in Sundaland?. Quaternary Sci Rev. 2005, 24: 2228-2242. 10.1016/j.quascirev.2005.04.004.View Article
- Louys J, Meijaard E: Palaeoecology of southeast Asian megafauna-bearing sites from the Pleistocene and a review of environmental changes in the region. J Biogeogr. 2010, 37 (8): 1432-1449.
- Mollengraaff GAF: Modern deep-sea research in the east Indian archipelago. Geogr J. 1921, 57: 95-121. 10.2307/1781559.View Article
- Bruyn M, Nugroho E, Hossain MM, Wilson JC, Mather P: Phylogeographic evidence for the existence of an ancient biogeographic barrier: the Isthmus of Kra Seaway. Heredity. 2005, 94: 370-378. 10.1038/sj.hdy.6800613.PubMedView Article
- Hamada Y, Suryobroto B, Goto S, Malaivijitnond S: Morphological and body color variation in Thai Macaca fascicularis fascicularis north and south of the Isthmus of Kra. Int J Primatol. 2008, 29: 1271-1294. 10.1007/s10764-008-9289-y.View Article
- Heaney LR: Biogeography of mammals in SE Asia: estimates of rates of colonization, extinction and speciation. Biol J Linnean Soc. 1986, 28: 127-165. 10.1111/j.1095-8312.1986.tb01752.x.View Article
- Hughes JB, Round PD, Woodruff DS: The Indochinese–Sundaic faunal transition at the Isthmus of Kra: an analysis of resident forest bird species distributions. J Biogeogr. 2003, 30: 569-580. 10.1046/j.1365-2699.2003.00847.x.View Article
- Pauwels OSG, David P, Chimsunchart C, Thirakhupt K: Reptiles of Phetchaburi Province, Western Thailand: a list of species, with natural history notes, and a discussion on the biogeography at the Isthmus of Kra. Nat Hist J Chulalongkorn Univ. 2003, 3 (1): 23-53.
- Smith DR, Villafuerte L, Otis G, Palmer MR: Biogeography of Apis cerana F. and A. nigrocincta Smith: insights from mtDNA studies. Apidologie. 2000, 31: 265-279. 10.1051/apido:2000121.View Article
- Turner H, Hovenkamp P, Welzen PC: Biogeography of Southeast Asia and the West Pacific. J Biogeogr. 2001, 28: 217-230.View Article
- Wishart MJ, Hughes JM: Genetic population structure of the net-winged midge, Elporia barnadi (Diptera: Blephariceridae) in streams of the south-western Cape, South Africa: implications for dispersal. Freshwater Biol. 2003, 48: 28-38. 10.1046/j.1365-2427.2003.00958.x.View Article
- Baker AM, Hughes JM, Dean JC, Bunn SE: Mitochondrial DNA reveals phylogenetic structuring and cryptic diversity in Australian freshwater macroinvertebrate assemblages. Mar Freshwater Res. 2004, 55: 629-640. 10.1071/MF04050.View Article
- Finn DS, Adler PH: Population genetic structure of a rare high-elevation black fly, Metacnephia coloradensis, occupying lake outlet streams. Freshwater Biol. 2006, 51: 2240-2251. 10.1111/j.1365-2427.2006.01647.x.View Article
- Krosch MN: Phylogeography of Echinocladius martini Cranston (Diptera: Chironomidae) in closed forest streams of eastern Australia. Aust J Entomol. 2011, 50: 258-268.
- Krosch MN, Baker AM, McKie BG, Mather PB, Cranston PS: Deeply divergent mitochondrial lineages reveal patterns of local endemism in chironomids of the Australian Wet Tropics. Austral Ecol. 2009, 34: 317-328. 10.1111/j.1442-9993.2009.01932.x.View Article
- Smith PJ, McVeagh SM, Collier KJ: Population-genetic structure in the New Zealand caddisfly Orthopsyche fimbriata revealed with mitochondrial DNA. New Zeal J Mar Fresh. 2006, 40: 141-148. 10.1080/00288330.2006.9517408.View Article
- Ballard JWO DMR: The population biology of mitochondrial dna and its phylogenetic implications. Annu Rev Ecol Evol Syst. 2005, 36: 621-642. 10.1146/annurev.ecolsys.36.091704.175513.View Article
- Galtier N, Nabholz B, Glémin S, Hurst GDD: Mitochondrial DNA as a marker of molecular diversity: a reappraisal. Mol Ecol. 2009, 18: 4541-4550. 10.1111/j.1365-294X.2009.04380.x.PubMedView Article
- Ballard JWO, Whitlock MC: The incomplete natural history of mitochondria. Mol Ecol. 2004, 13: 729-744. 10.1046/j.1365-294X.2003.02063.x.PubMedView Article
- Rohlf FJ, Marcus LF: A revolution in morphometrics. Trends Ecol Evol. 1993, 8 (4): 129-132. 10.1016/0169-5347(93)90024-J.View Article
- Rohlf FJ: Shape statistics: Procrustes superimpositions and tangent spaces. J Classif. 1999, 16: 197-223. 10.1007/s003579900054.View Article
- Aytekin AM, Terzo M, Rasmont P, Çağatay N: Landmark based geometric morphometric analysis of wing shape in Sibiricobombus Vogt (Hymenoptera: Apidae: Bombus Latreille). Ann Soc Entomol Fr. 2007, 43 (1): 95-102.
- Bouyer J, Ravel S, Jujardin J-P, Meeüs T, Vial L, Thévenon S, Guerrini L, Sidibé I, Solano P: Population structuring of Glossina palpalis gambiensis (Diptera: Glossinidae) according to landscape fragmentation in the Mouhoun River, Burkina Faso. J Med Entomol. 2007, 44 (5): 788-795. 10.1603/0022-2585(2007)44[788:PSOGPG]2.0.CO;2.PubMedView Article
- Dujardin J-P, Pont FL, Baylac M: Geographical versus interspecific differentiation of sand flies (Diptera: Psychodidae): a landmark data analysis. Bull Entomol Res. 2003, 93: 87-90.PubMedView Article
- Gilchrist AS, Crisafulli DCA: Using variation in wing shape to distinguish between wild and mass-reared individuals of Queensland fruit fly, Bactrocera tryoni. Entomol Exp Appl. 2006, 119: 175-178. 10.1111/j.1570-7458.2006.00395.x.View Article
- Schutze MK, Jessup A, Clarke AR: Wing shape as a potential discriminator of morphologically similar pest taxa within the Bactrocera dorsalis species complex (Diptera: Tephritidae). Bull Entomol Res. 2011, 102 (1): 103-111.PubMedView Article
- D’Anatro A, Lessa EP: Geometric morphometric analysis of geographic variation in the Río Negro tuco-tuco, Ctenomys rionegrensis (Rodentia: Ctenomyidae). Mamm Biol. 2006, 71: 288-298. 10.1016/j.mambio.2006.02.001.
- Francoy TM, Wittmann D, Steinhage V, Drauschke M, Müller S, Cunha DR, Nascimento AM, Figueiredo VLC, Simões ZLP, Jong DD, et al: Morphometric and genetic changes in a population of Apis mellifera after 34 years of Africanization. Genet Mol Res. 2009, 8 (2): 709-717. 10.4238/vol8-2kerr019.PubMedView Article
- Arbogast BS, Edwards SV, Wakeley JW, Beerli P, Slowinski JB: Estimating divergence times from molecular data on phylogenetic and population genetic timescales. Annu Rev Ecol Syst. 2002, 33: 707-740. 10.1146/annurev.ecolsys.33.010802.150500.View Article
- Zarowiecki M, Walton C, Torres E, McAlister E, Htun PT, Sumrandee C, Sochanta T, Dinh TH, Ng LC, Linton YM: Pleistocene genetic connectivity in a widespread, open-habitat-adapted mosquito in the Indo-Oriental region. J Biogeogr. 2011, 38: 1422-1432. 10.1111/j.1365-2699.2011.02477.x.View Article
- O'Higgins P, Jones N: Morphologika, tools for statistical shape analysis. 2006, Hull York Medical School, University of York, York
- Klingenberg CP: MorphoJ: an integrated software package for geometric morphometrics. Mol Ecol Resour. 2011, 11: 353-357. 10.1111/j.1755-0998.2010.02924.x.PubMedView Article
- Drake AG, Klingenberg CP: The pace of morphological change: Historical transformation of skull shape in St. Bernard dogs. Proc R Soc B. 2008, 275: 71-76.PubMedView Article
- Wright S: Isolation by distance. Genetics. 1943, 28 (2): 114-138.PubMedPubMed Central
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R: DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994, 3: 294-299.PubMed
- Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999, 41: 95-98.
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol Biol Evol. 2007, 24 (8): 1596-1599. 10.1093/molbev/msm092.PubMedView Article
- Excoffier L, Laval G, Schneider S: Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online. 2005, 1: 47-50.PubMed Central
- Bandelt H-J, Forster P, Rohl A: Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol. 1999, 16: 37-48. 10.1093/oxfordjournals.molbev.a026036.PubMedView Article
- Tamura K, Nei M: Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993, 10: 512-526.PubMed
- Excoffier L, Smouse PE, Quattro JM: Analysis of molecular variance inferred from metric distances among DNA haplotypes: applications to human mitochondrial DNA restriction data. Genetics. 1992, 131: 479-491.PubMedPubMed Central
- Lessa EP: Multidimensional analysis of geographic genetic structure. Syst Zool. 1990, 39 (3): 242-252. 10.2307/2992184.View Article
- Fu Y: Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics. 1997, 147: 915-925.PubMedPubMed Central
- Librado P, Rozas J: DNAsp v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009, 25: 1451-1452. 10.1093/bioinformatics/btp187.PubMedView Article
- Minin VN, Bloomquist EW, Suchard MA: Smooth Skyride through a rough Skyline: Bayesian coalescent-based inference of population dynamics. Mol Biol Evol. 2008, 25 (7): 1459-1471. 10.1093/molbev/msn090.PubMedPubMed CentralView Article
- Drummond AJ, Rambaut A: BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol. 2007, 7: 214-10.1186/1471-2148-7-214.PubMedPubMed CentralView Article
- Wahlberg N: That awkward age for butterflies: Insights from the age of the butterfly subfamily Nymphalinae (Lepidoptera: Nymphalidae). Syst Biol. 2006, 55: 703-714. 10.1080/10635150600913235.PubMedView Article
- Zakharov EV, Caterino MS, Sperling FAH: Molecular phylogeny, historical biogeography, and divergence time estimates for swallowtail butterflies of the genus Papilio (Lepidoptera: Papilionidae). Syst Biol. 2004, 53: 193-215. 10.1080/10635150490423403.PubMedView Article
- Brower AV: Rapid morphological radiation and convergence among races of the butterfly Heliconius erato inferred from patterns of mitochondrial DNA evolution. Proc Natl Acad Sci USA. 1994, 91 (14): 6491-6495. 10.1073/pnas.91.14.6491.PubMedPubMed CentralView Article
- Lemey P, Rambaut A, Drummond AJ, Suchard MA: Bayesian phylogeography finds its roots. PLoS Comput Biol. 2009, 5: e1000520-10.1371/journal.pcbi.1000520.PubMedPubMed CentralView Article
- Bielejec F, Rambaut A, Suchard MA, Lemey P: SPREAD: Spatial Phylogenetic Reconstruction of Evolutionary Dynamics. Bioinformatics. 2011, 27 (20): 2910-2912. 10.1093/bioinformatics/btr481.PubMedPubMed CentralView Article
- Clarke AR, Allwood A, Chinajariyawong A, Drew RAI, Hengsawad C, Jirasurat M, Krong CK, Kritsaneepaiboon S, Vijaysegaran S: Seasonal abundance and host use patterns of seven Bactrocera Macquart species (Diptera: Tephritidae) in Thailand and Peninsuar Malaysia. Raffles Bull Zool. 2001, 49 (2): 207-220.
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