Indehiscent sporangia enable the accumulation of local fern diversity at the Qinghai-Tibetan Plateau
- Li Wang†1, 2, 3,
- Harald Schneider†1, 2,
- Zhiqiang Wu1, 3,
- Lijuan He1, 3,
- Xianchun Zhang1 and
- Qiaoping Xiang1Email author
© Wang et al.; licensee BioMed Central Ltd. 2012
Received: 27 March 2012
Accepted: 27 August 2012
Published: 28 August 2012
Indehiscent sporangia are reported for only a few of derived leptosporangiate ferns. Their evolution has been likely caused by conditions in which promotion of self-fertilization is an evolutionary advantageous strategy such as the colonization of isolated regions and responds to stressful habitat conditions. The Lepisorus clathratus complex provides the opportunity to test this hypothesis because these derived ferns include specimens with regular dehiscent and irregular indehiscent sporangia. The latter occurs preferably in well-defined regions in the Himalaya. Previous studies have shown evidence for multiple origins of indehiscent sporangia and the persistence of populations with indehiscent sporangia at extreme altitudinal ranges of the Qinghai-Tibetan Plateau (QTP).
Independent phylogenetic relationships reconstructed using DNA sequences of the uniparentally inherited chloroplast genome and two low-copy nuclear genes confirmed the hypothesis of multiple origins of indehiscent sporangia and the restriction of particular haplotypes to indehiscent sporangia populations in the Lhasa and Nyingchi regions of the QTP. In contrast, the Hengduan Mountains were characterized by high haplotype diversity and the occurrence of accessions with and without indehiscent sporangia. Evidence was found for polyploidy and reticulate evolution in this complex. The putative case of chloroplast capture in the Nyingchi populations provided further evidence for the promotion of isolated but persistent populations by indehiscent sporangia.
The presented results confirmed the hypothesis that indehiscent sporangia promote the establishment of persistent population in different regions of the QTP. These results are consistent with the expectations of reproductive reassurance by promotion of self-fertilization that played a critical role in the assembly of populations in isolated locations and/or extreme habitats.
KeywordsChloroplast capture Chloroplast DNA Himalaya Incongruent phylogenetic hypotheses Lepisorus clathratus Phylogenetic analyses Reticulate evolution Self-fertilization Low-copy nuclear genes PgiC LFY
Several recent studies suggested that environmental condition enforced the evolution of self-fertilization in plants, especially as a strategy to adapt to extreme habitats  and along the trailing edge . For example, evidence was reported for indirect selection of mating system during the evolution of drought resistance in the angiosperm genus Mimulus evolution . These studies add alternative aspects to the well-established hypothesis of reproductive assurance as a cause for the establishment of self-fertilizing populations in plants . Recent studies on the biology of fern dispersal, in particular long-distance dispersal emphasized evidence for a correlation of colonization of new locations and the capacity of self-fertilization [5–7].
The majority of ferns appear to reproduce either via out-crossing or mixed mating systems [6, 8–11], but several mechanisms exist to promote inbreeding in ferns . The catapult mechanisms of the sporangia, as found in the vast majority of species belonging to Polypodiales , are one of the most prominent features among derived ferns promoting dispersal  and subsequently out-crossing. However, a handful or so exceptions, which lack such catapult mechanisms, have been reported among derived ferns, e.g. some European spleenworts such as the tetraploid Asplenium lepidum[15, 16] and the diploid Asplenium jahandiezii. These sporangia are called throughout this paper ‘indehiscent sporangia’ whereas regular sporangia are called ‘dehiscent sporangia’. Very little is known about the origin of the evolutionary processes supporting the establishment of indehiscent sporangia. Here, we explore the hypothesis that indehiscent sporangia promote self-fertilization coinciding with geographical isolation, local survival, and adaptation to extreme environmental conditions.
To study this hypothesis, we explore the evolution of indehiscent sporangia in ferns occurring at the high altitudes of the Qinghai-Tibetan plateau (QTP), also called the “roof of the world”. Specimens with indehiscent sporangia belonging to the Lepisorus clathratus complex were first reported as the segregate genus Platygyria[18, 19]. However, recent studies confirmed that these specimens belong to the Lepisorus clathratus complex [20–22]. Thus, this complex comprises not only specimens with regularly formed, dehiscent sporangia that open with the above mentioned catapult mechanism, but also specimens with irregularly formed, indehiscent sporangia. Besides, existing records show evidences for predominant occurrence of specimens with indehiscent sporangia at high altitudes >3,000 meter in contrast to specimens with dehiscent sporangia occurring at lower altitudes [19, 22, 23]. Indehiscent sporangia promoting self-fertilization may be an adaptation to the isolation of suitable habitats, extreme climatic windy conditions, and short vegetation periods in the extreme altitudes of the QTP. Consequently, several authors suggested an origin of Platygyria, = specimens with indehiscent sporangia, from Lepisorus clathratus complex, = specimens with dehiscent sporangia, during the uplift of the Himalaya [18, 19, 23]. Indehiscent sporangia are assumed to be advantageous to secure sexual reproduction, = reproductive assurance, in conditions of strong isolation of putative habitats. Thus, adaptive advantages of self-fertilization may explain multiple origins of indehiscent sporangia in the Lepisorus clathratus complex . To avoid taxonomic confusion, the complex is treated as a single species, L. clathratus, throughout this paper. Platygyria is treated as a synonym of Lepisorus[20, 21].
This study is carried out in the context of previously reported evidences suggesting multiple origins of indehiscent sporangia . The previous report was based exclusively on maternally inherited chloroplast genome sequences (cpDNA) [24, 25] and thus it was unable to account for introgression, reticulation, and polyploidy [26–28]. Polyploidy and the indication for reticulate evolution have been recorded for the genus Lepisorus and the most recently also for the L. clathratus complex . Thus, we explore the evolution of indehiscent sporangia by using sequence data of two cpDNA markers and two nuclear low-copy genes, LFY and PgiC, plus flow cytometry to determine the DNA ploidy level . Finally, we expanded our sampling by including specimens with indehiscent sporangia collected in the Nyingchi prefecture, located in the southeastern part of Tibet Autonomous Region of China. The specimens will be named throughout this paper as Nyingchi Platygyria. The study is designed to provide a more comprehensive insight in the spatial-phylogeographic origin of indehiscent sporangia in the Lepisorus clathratus complex and the ecological-evolutionary consequences. Together with the Lhasa region, the Nyingchi region is known for the abundance of specimens with indehiscent sporangia compared to the Hengduan Mountains where specimens with indehiscent sporangia are less common than those with dehiscent sporangia.
Samples were obtained via fieldwork by members of the research team in various parts of China and neighboring countries. Materials for DNA extraction were dried and stored in silica. Some plants were collected as living collections and cultivated at the conservatory of Institute of Botany, Chinese Academy of Sciences (IBCAS). Taxon sampling was guided by recently reported phylogenetic analyses [20–22] to include the resolved clades within Lepisorus, but particular care was taken to cover the geographic range of all morphologically distinct forms of Platygyria. The two genera Neocheiropteris and Tricholepidium were sampled to have a glimpse of the phylogenetic relationships of Lepisorus and its allies.
Whole genomic DNA was extracted using a modified CTAB approach . These DNA extracts were used to amplify two chloroplast regions rps4-trnS and trnL trnF (consisting of rps4 + rps4 trnS intergenic spacer and trnL intron plus trnL F intergenic spacer) as described in Wang et al. . The DNA was also used to amplify two nuclear genes, the low-copy gene PgiC using the primers and protocol of Ishikawa et al. and the low-copy gene LFY using newly designed LFY primers for the introns located between exon1 to exon 3: FLFYE1dF: 5’-GGCAACGCCTRCAACTACT-3’; FLFYE3dR: 5’-CTTTGGYTTGTTGATRTACT-3’; FLFYE3eR: 5’-GCRTGTCGAAAAACYTGRTTGGT-3’. To separate the different allele copies, we performed cloning experiments using pGEM T-easy vectors (Promega Corp.) with a Pharmacia purification kit (Amersham Pharmacia Biotech) following the manufactures’ protocols. Four to seven clones of LFY gene were sequenced for the selected 12 specimens. Cloning and sequencing were applied on PgiC gene of 25 specimens (2–7 clones) which represented the key genotypes resolved in our previous study . PgiC gene of the remaining 23 specimens was directly sequenced in order to reduce experimental expense. Sequencing reactions were carried out using the DYEnamicTM ETDye Terminator Cycle Sequencing Kit (Amersham Pharmacia Biotech). Sequences were analyzed using MegaBACETM1000 DNA Analysis Systems, following the manufacture’s protocols.
All newly generated sequences were assembled and edited using ContigExpress program from the Vector NTI Suite 6.0 (Informax Inc., North Bethesda, MD). BlastN searches were carried out for all newly generated sequences . Alignments were generated using CLUSTAL X , and further adjusted manually in BioEdit  and MacClade 4.0 . Ambiguously aligned regions were detected visually and excluded from further analyses. Only two small ambiguously aligned fragments were detected in the cpDNA (<3% of the total base pairs) sequences and none in the nuclear regions. Searches for sequence inversions were negative. For the two nuclear genes, we took into consideration of PCR bias, the putative occurrence of chimaeric sequences, and variation due to polymerase error by checking the sequences very carefully and blast sequences in NCBI (http://www.ncbi.nlm.nih.gov). The copy numbers of the two nuclear genes (PgiC and LFY) in each specimen were not identified because the ploidy levels of most studied samples are uncertain. However, all analyses were carried out including all cloned sequences and with a reduced dataset including only sequences found in more than one clone per specimen. Besides newly generated sequences (all nuclear gene sequences and minority of chloroplast sequences), we used sequences that were generated for previous studies [20–22] and all newly generated sequences were deposited in GenBank (see Additional file 1). In total, we analyzed four datasets independently. The first dataset includes all cpDNA sequences, whereas the second and third datasets correspond to the two low-copy gene datasets LFY and PgiC, and the fourth dataset is constituted of the reduced PgiC sequences as mentioned above.
Gene-trees were reconstructed using standard phylogenetic methods such as maximum parsimony in PAUP 4.0b10 , maximum likelihood in Garli 1.0  and PHYML 3.0 , and Bayesian inference in MrBayes 3.1.2 . jModeltest 0.1.1  was used to determine the model of sequence parameters for model-based approaches as maximum likelihood and Bayesian inference. Non-parametric bootstrap values were calculated for maximum parsimony with 1,000 bootstrap replicates and maximum likelihood with 500 replicates. All analyses were carried out using standard protocols as described in our previous studies [20–22] and the phylogenetic trees were visualized using FigTree 1.3.1. (http://tree.bio.ed.ac.uk/software/figtree). Convergence of MCMC runs from Bayesian analyses were also studied using Tracer 1.5 (http://tree.bio.ed.ac.uk/software/tracer). The burn-in phase was defined manually by studying carefully the output of TRACER. The estimated burn-in phase comprised fewer generations than the default 10% cut-off implemented in this software. Phylogenetic network analyses [43, 44] were carried out using SPLITSTREE 4.10 (http://www.splitstree.org) and DENDROSCOPE 3.0.13beta (http://ab.inf.uni-tuebingen.de/softwar/dendroscope). These methods were used to visualize and infer alternative hypothesis of reticulate evolution in combination with established procedures (SH-test) and collapsing multiple trees (in DENDROSCOPE) and z-closure consensus networks (in SPLITSTREE). These methods were applied to distinguish between phylogenetic uncertainties and reticulate evolution; in particular we compared very carefully the signals given by different phylogenetic trees either visually in DENDROSCOPE or by calculating z-closure networks in SPLITSTREE. These analyses were carried out for three kinds of tree sets obtained by individual analyses of each of the three regions: (1) all most parsimonious trees recovered in maximum parsimony analyses, (2) all most likely trees recovered in maximum likelihood analyses, and (3) 100 bayesian trees recovered in Bayesian inference of phylogeny. Node support was further investigated using additional tests as implemented in PAUP, e.g. SH-test . Regional diversity was evaluated by considering specimens and sequences used in this study and previous studies on the Lepisorus clathratus complex [20–22].
Four samples, representing the main distribution range of L. clathratus complex  and including the special “Nyingchi Platygyria”, were selected to explore ploidy levels because existing chromosome counts suggest the occurrence of diploids and tetraploids in the L. clathratus complex [30, 46]. In the absence of materials suitable for additional chromosome counts, we employed flow cytometry [47, 48] to determine the DNA ploidy level . These values were determined using silica dried leaf material analyzed together with the internal standard consisting of leaf material of a specimen that was reported to be tetraploid . The leaf material was chopped together with the internal standard, = tetraploid Lepisorus clathratus s.l., in 0.5 ml ice-cold general-purpose buffer with the addition of 3% PVP-40 . The nuclear suspension was filtered through a nylon mesh and then incubated after adding a solution containing ribonuclease A (RNase A; Sigma-Aldrich, St. Louis, MI, USA). Finally, samples were stained with propidium iodide (Sigma-Aldrich, St. Louis, Missouri, USA) and incubated on ice for 30 minutes before analyzed using a BD FACSCaliburTM flow cytometer (BD Bioscience, Franklin Lakes, NJ, USA). For each specimen, we counted about 40,000 nuclei per run. Only measurements were considered with a clear signal. The DNA ploidy level was recorded as relative to the internal tetraploid standard: < 4x, = 4x, > 4x.
Genetic variation of chloroplast genome of specimens with indehiscent sporangia for three core distribution regions
Lhasa region *
Nyingchi region *
39 / 0
6 / 0
23 / 29
3 / 0
1 / 0
3 / 4
6 / 0
3 / 0
8 / 9
Evidence for chloroplast capture
Strong conflicts were found between the phylogenetic hypotheses obtained using chloroplast and nuclear DNA sequence data (Figures 4). These conflicts can be sorted into two categories. The first category, conflicts such as the position of the genera Neocheiropteris and Tricholepidium may be the result of insufficient information or saturated variation in one or both datasets (Figures 4). This argument may especially apply to the highly variable regions used to reconstruct the phylogeny, as both LFY and PgiC markers are mainly based on intron data. Thus, the high substitution rate of these non-coding regions may show effects of sequence saturation. This argument is also consistent with the low support values recovered for the majority of the deep nodes using these markers (Figures 2, 3), but it also applies to the cpDNA (Figures 1). The second category, the conflict concerning the position of Platygyria specimens from the Nyingchi region, cannot be explained by these arguments. These specimens have LFY and PgiC sequences identical or nearly identical to specimens found in the Lepisorus clathratus clade (Figures 2, 3, 5), whereas the haplotypes of chloroplast sequences recovered in the Nyingchi Platygyria specimens are unique and have not been detected in any studied species of Lepisorus or related genera of lepisoroid or other ferns (Figures 1). In order to rule out any issue of contamination or related experimental errors, we performed additional independent DNA extractions, amplifications and sequencing of several specimens, using five specimens collected in the Nyingchi region and one collected between Maizhokunggar and Gongbo’gyamda located between Lhasa and Nyingchi. .
The cpDNA phylogeny suggests a long time isolation of this chloroplast type found in the Nyingchi specimens (Figures 1) and thus the results are not compatible with the hypothesis of incomplete lineage sorting. In the maximum parsimony analyses, they were recovered as sister lineage to Paragramma, but this may be the result of long-branch attraction. Our results of the cpDNA and nrDNA data suggest independent inheritance of the two genomes. They are also contrast with other collections of Platygyria (with indehiscent sporangia), which have both cpDNA and nrDNA nested within the Lepisorus clathratus clade (Figures 1, 2, 3).
The hypothesis of chloroplast capture provides the most likely explanation for the recovered conflict between the phylogenetic results of the cpDNA and nrDNA inheritance. The origin of the Nyingchi genotype is assumed to involve initial hybridization between individuals of the Platygyria type and an unknown species of Lepisorus. In this scenario, this unknown parent contributed the chloroplast because the cpDNA is maternally inherited in ferns [24, 25]. Consequently, the nuclear genome is inherited paternally as required in processes involving chloroplast capture [50, 51]. Remarkably, the cpDNA of the Nyingchi Platygyria specimens has not been recorded in any species of Lepisorus and relatives despite a rather comprehensive sampling in previous studies [20, 21]. Morphological studies do not suggest any unique species of Lepisorus or its relatives in Nyingchi region. However, previous studies provided evidence that the reproduction with indehiscent sporangia, can result in populations with locality-persistent occurrences for considerably long time . Thus, the combination of geographic isolation and indehiscent sporangia may have contributed to the origin and fixation of the Nyingchi Platygyria genotype.
The presented results are only the second report of putative chloroplast capture in ferns . However, this may not necessarily indicate that the probability of chloroplast capture in ferns is low because only a small number of phylogenetic studies on ferns have integrated both chloroplast and low-copy nuclear genes so far [53–59].
Evolution of indehiscent sporangia
Similar to the cpDNA, indehiscent sporangia are not associated with a single copy or clade of copies of PgiC (Figures 2), which provided further evidences for multiple origin of indehiscent sporangia. However, the pattern is also consistent with the hypothesis of indehiscent sporangia caused by mutations through recombination, introgression or hybridization between specimens with and without indehiscent sporangia. The above discussed chloroplast capture provided further evidence to this hypothesis of introgression of specimens with and without indehiscent sporangia. In addition, this study provides evidences for ploidy level changes, which supports multiple origins via hybridization combined with polyploidy as the most likely scenario although one specimen (no. 119) of Platygyria was found to be probably a diploid (see Figures 6). The argument on recombination is based on the reproductive biology of specimens with indehiscent sporangia (Platygyria type). Sporangia without the catapult mechanisms promote self-fertilization among the gametophytes developed from spores formed by meiosis events within a single sporangium. Actually, the mechanism enhances the probability that egg cells of archegonia of these gametophytes are fertilized by sperm cells developed in antheridia formed by gametophytes originated from the same sporangium. Thus, the self-fertilization may be either inter-gametophytic or intra-gametophytic . At the same time, the sperm cells of gametophytes formed from spores originated within a single indehiscent sporangium may be able to fertilize gametophytes of individuals shedding their spores via dehiscent sporangia. Thus, the character of indehiscent sporangia may be inherited by zygotes that are formed by the fertilization of egg cells of gametophytes originated from sporophytes with dehiscent sporangia and sperm cells of gametophytes originated from sporophytes with indehiscent sporangia. This hypothesis proposes a linkage between paternal inheritance and indehiscent sporangia. The above discussed evidence for chloroplast capture supports this hypothesis because cpDNA is maternally inherited whereas the nuclear DNA is paternally inherited.
Previous studies on the allotetraploid Asplenium lepidum[15, 16] have provided evidence for the origin of taxa with indehiscent sporangia via hybridization and polyploidy. Our results are consistent with the hypothesis that the origin of indehiscent sporangia involves these processes. However, the current estimates of ploidy levels are insufficient to prove that the reticulate pattern observed between the cpDNA and nrDNA is the result of hybridization and polyploidy, not caused by recombination among specimens with the same ploidy level.
However, the establishment of indehiscent sporangia is not necessarily linked to these processes as illustrated by the diploid Asplenium jahandiezii. This taxon is endemic to a small region in southeastern France, the Gorge du Verdon. The sister species, A. bourgaei is also diploid but distinct by dehiscent sporangia and an occurrence in the eastern Mediterranean . Both species are considered Tertiary relicts and current range of Asplenium jahandiezii is part of a region considered as Pleistocene glacial refugia of ferns and other plants [60–62]. Thus, indehiscent sporangia may have successfully promoted local survival of this species in a rather small and well-defined region of southeastern France.
Local survival through the last glacial maximum has also been discussed for occurrences of Lepisorus clathratus complex with indehiscent sporangia (Platygyria) at the QTP . The newly discovered evidence for an isolated taxon in the Nyingchi region, Nyingchi Platygyria, adds a further support for the emerging hypothesis that self-fertilization promotes persistence of populations despite isolation and environmental challenges [1, 2].
Consequences for taxonomic research
This study sheds new light on the difficult taxonomy of the Lepisorus clathratus complex and in particular the treatment of Platygyria. The latter was recently reduced to be a synonym of Lepisorus. Here, we found further support that the majority of Platygyria genotypes are intermingled with Lepiosorus clathratus genotypes [20, 22]. However, the new results suggesting different ploidy levels support the hypothesis that the complex comprises at least two diploid entities that contributed to the origin of polyploids via hybridization. Future studies will need to focus on the untangling of the reticulate evolution that may be comparable to complexes such as Asplenium lepidum and its diploid parents, A. aegeum and A. dolomiticum[15, 16].
Without doubt, the specimens treated here as Nyingchi Platygyria form a separate taxonomic entity. Future work will need to address the taxonomic implications of this study, such as the identification of the species name for these specimens from several names established for species with indehiscent sporangia [18, 19].
Assembly of plant diversity on the roof-of-the-world
In the recent years, many studies addressed aspects of the evolution of plants on the roof-of-the-world. Some of these studies resemble our own results in pointing out the remarkable evolution of unusual traits in adaptation to the alpine environments such as colored bracts  and the cushion growth forms  in various genera of eudicots. In our opinion, indehiscent sporangia may be such a selective advantageous character.
The report of the Nyingchi area as a putative mixture zone and survival area is also highly relevant to the current discussion on the history of plant diversity at this region in the last 4–5 million years . In particular, the documentation of the Nyingchi area as survival area (refugia) is consistent with results on other plants such as Cupressus and Mecanopsis[66, 67]. Furthermore, our result resembles the study on Mecanopsis in the documentation of the establishment of hybrid taxa in isolated locations along the southern border of the species range . Establishment of polyploid hybrids appears to be a rather common process in the response of alpine plants to glacial cycles not only in the Himalaya but also in other areas such as the European Alps .
The study found evidence that indehiscent sporangia promote the persistence of circumstances despite isolation and challenging climatic conditions in the Himalaya. Some of the evidence was based on the observation of a unique chloroplast genome in specimens with indehiscent sporangia collected in the isolated Nyingchi region. The origin of this chloroplast is best explained by chloroplast capture. Future research needs to focus on two core approaches: 1) additional data are required to reconstruct the contribution of reticulation and polyploidy; 2) sampling of data should allow reconstructing the population history of these taxa in three regions discussed: Hengduan Mts, Lhasa and Nyingchi regions. In general, these results raise questions concerning the contribution of shifts in the mating system to the origin of the unique plant diversity of the Himalaya.
We thank Miss Hui-Juan Zhao and Prof. Xiao-Quan Wang (IBCAS) for allowing us to use the LFY primers designed in Zhao’s master thesis. Financial support was provided by the National Natural Science Foundation of China (NSFC No. 31070196, No. 31070182 and No. 30870167). HS acknowledges the support from the Chinese Academy of Sciences (visiting professorship).
- Levin DA: Environment-enhanced self-fertilization: implications for niche shifts in adjacent populations. J Ecol. 2010, 98: 1276-1283. 10.1111/j.1365-2745.2010.01715.x.View Article
- Levin DA: Mating system shifts on the trailing edge. Ann Bot. 2012, 109: 613-620. 10.1093/aob/mcr159.PubMedPubMed CentralView Article
- Ivey CT, Carr DE: Tests for the joint evolution of mating system and drought escape in Mimulus. Ann Bot. 2012, 109: 583-598. 10.1093/aob/mcr160.PubMedPubMed CentralView Article
- Busch JW, Delph LF: The relative importance of reproductive assurance and automatic selection as hypotheses for the evolution of self-fertilization. Ann Bot. 2012, 109: 553-562. 10.1093/aob/mcr219.PubMedPubMed CentralView Article
- Cameron EK, de Lange PJ, Perrie LR, Brownsey PJ, Cambell HJ, Taylor GA, Given DR, Bellingham RM: A new location for the poor knights spleenwort (Asplenium pauperequitum, Aspleniaceae) on the Forty Fours, Chatham Islands, New Zealand. New Zeal J Bot. 2006, 44: 199-209. 10.1080/0028825X.2006.9513018.View Article
- Wubs ERJ, de Groot GA, During HJ, Vogel JC, Grundmann M, Bremer P, Schneider H: Mixed mating system in the fern Asplenium scolopendrium: implications for colonization potential. Ann Bot. 2010, 106: 583-590. 10.1093/aob/mcq157.PubMedPubMed CentralView Article
- De Groot GA, Verduyn B, Wubs ERJ, Erkens RHJ, During HJ: Inter- and intraspecific variaiton in fern mating systems after long-distance colonization: the importance of selfing. BMC Plant Biol. 2012, 12: 3-10.1186/1471-2229-12-3.PubMedPubMed CentralView Article
- Soltis PS, Soltis DE: Evolution of inbreeding and outcrossing in ferns and fern allies. Plant Spec Biol. 1990, 5: 1-11. 10.1111/j.1442-1984.1990.tb00187.x.View Article
- Soltis DE, Soltis PS: The distribution of selfing rates in homosporous ferns. Am J Bot. 1992, 79: 97-100. 10.2307/2445202.View Article
- Haufler CH: Homospory 2002: An odyssey of progress in pteridophyte genetics and evolution biology. BioScience. 2002, 52: 1081-1093. 10.1641/0006-3568(2002)052[1081:HAOOPI]2.0.CO;2.View Article
- Jesson LK, Garnock-Jones PJ: Can classifications of functional gender be extended to all land plants?. Plant Ecol Evol Syst. 2011, 10.1016/j.ppees.2011.10.003..
- Klekowski EJ: The genetics and reproductive biology of ferns. The Experimental Biology of Ferns. Edited by: Dyer AF. 1979, London: Academic Press, 133-170.
- Schneider H, Smith AR, Pryer KM: Is morphology really at odds with molecules in estimating fern phylogeny?. Syst Bot. 2009, 34: 455-475. 10.1600/036364409789271209.View Article
- Noblin X, Rojas NO, Westbrook J, Llorens C, Argentina M, Dumais J: The fern sporangium: a unique cataput. Science. 2012, 335: 1322-10.1126/science.1215985.PubMedView Article
- Brownsey PJ: A biosystematic study of the Asplenium lepidum complex. Bot J Linn Soc. 1976, 72: 235-267. 10.1111/j.1095-8339.1976.tb01363.x.View Article
- Brownsey PJ: An example of sporangila indehiscence in the Filicopsida. Evolution. 1977, 31: 294-301. 10.2307/2407750.View Article
- Schulze G, Treutlein J, Wink M: Genetic relationships between Asplenium bourgaei (Boiss.) and A. jahandiezii (Litard.) Rouy inferred from morphological characters and rbcL sequences. Pl Biol. 2001, 3: 364-371. 10.1055/s-2001-16464.View Article
- Ching RC, Wu SK: Platygyria Ching et S.K. Wu, a unique new genus of the Polypodiacae from China. Acta Bot Yunnanica. 1980, 2: 67-74.
- Zhang XC, Liu QR, Xu J: Systematics of Platygyria Ching & S.K.Wu (Polypodiaceae). Acta Phytotax Sin. 2003, 41: 401-415.
- Wang L, Qi XP, Xiang QP, Heinrichs J, Schneider H, Zhang XC: Phylogeny of the paleotropical fern genus Lepisorus (Polypodiaceae, Polypodiopsida) inferred from four chloroplast genome regions. Mol Phylogenet Evol. 2010, 54: 211-225. 10.1016/j.ympev.2009.08.032.PubMedView Article
- Wang L, Wu ZQ, Xiang QP, Heinrichs J, Schneider H, Zhang XC: A molecular phylogeny and a revised classification of tribe Lepisoreae (Polypodiaceae) based on an analysis of four plastid DNA regions. Biol J Linn Soc. 2010, 162: 28-38. 10.1111/j.1095-8339.2009.01018.x.View Article
- Wang L, Wu ZQ, Bystriakova N, Ansell SW, Xiang QP, Heinrichs J, Schneider H, Zhang XC: Phylogeography of the Sino-Himalayan fern Lepisorus clathratus on “the roof of the world”. PLoS One. 2011, 6: e25896-10.1371/journal.pone.0025896.PubMedPubMed CentralView Article
- Ching RC, Wu SK: The floristic characteristics of the Xizang (Tibet) pteridophyte flora in relation to the upheaval of the Hiamalyas. Acta Bot Yunnanica. 1980, 2: 382-389.
- Gastony GJ, Yatskievych G: Maternal inheritance of the chloroplast and mitochondrial genomes in cheilanthoid ferns. Am J Bot. 1992, 79: 716-722. 10.2307/2444887.View Article
- Vogel JC, Russell SJ, Rumsey FJ, Barrett JA, Gibby M: Evidence for maternal transmission of chloroplast DNA in the genus Asplenium (Aspleniaceae, Pteridophyta). Bot Acta. 1998, 111: 247-249.View Article
- Rieseberg L, Soltis DE: Phylogenetic consequences of cytoplasmic gene flow in plants. Evol Trend Plant. 1991, 5: 65-84.
- Linder CR, Rieseberg LH: Reconstructing patterns of reticulate evolution in plants. Am J Bot. 2004, 91: 1700-1708. 10.3732/ajb.91.10.1700.PubMed CentralView Article
- Baack EJ, Rieseberg LH: A genomic view of introgression and hybrid speciation. Curr Opin Genet Dev. 2007, 17: 513-518. 10.1016/j.gde.2007.09.001.PubMedPubMed CentralView Article
- Shinohara W, Ushio Y, Seo A, Nakato N, Kono M, Udoh H, Tobe H, Murakami N: Evidence for hybrid orign and segmental allopolyploidy in eutetraploid and aneutetraploid Lepisorus thunbergianus (Polypodiaceae). Syst Bot. 2010, 35: 20-29. 10.1600/036364410790862498.View Article
- Qi XP, Zhang XC: Cytotaxonomic study of three species of Lepisorus from China (Polypodiaceae). Guihaia. 2011, 31: 181-183.
- Suda J, Krahulcova A, Travenicek P, Krahulec F: Ploidy level versus DNA ploidy level: an appeal for consistent terminology. Taxon. 2006, 55: 447-450. 10.2307/25065591.View Article
- Doyle JJ, Doyle JL: A rapid DNA isolation procedure for small amounts of fresh leaf tissue. Phytochem Bull. 1987, 19: 11-15.
- Ishikawa H, Watano Y, Kano K, Ito M, Kurita S: Development of primer sets for PCR amplification of the pgiC gene in ferns. J Plant Res. 2002, 115: 65-70. 10.1007/s102650200010.PubMedView Article
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.PubMedView Article
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25: 4876-4882. 10.1093/nar/25.24.4876.PubMedPubMed CentralView Article
- Hall TA: BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999, 41: 95-98.
- Maddison DR, Maddison WP: MacClade 4: analysis of phylogeny and character evolution, Version 4.08. 2005, Sinauer Associates, Sunderland, Massachusetts
- Swofford DL, PAUP, PAUP: PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4.0b10. 2002, Sinauer Associates, Sunderland, Mass
- Zwickl DJ: Genetic algorithm approaches for the phylogenetic analysis of arge biological sequence datasets under the maximum likelihood criterion. 2006, The University of Texas at Austin: Ph.D. dissertation
- Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O: New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 2010, 59: 307-321. 10.1093/sysbio/syq010.PubMedView Article
- Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19: 1572-1574. 10.1093/bioinformatics/btg180.PubMedView Article
- Posada D: jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008, 25: 1253-1256. 10.1093/molbev/msn083.PubMedView Article
- Huson DH, Bryant D: Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006, 23: 254-267.PubMedView Article
- Huson DH, Scornavacca C: A survey of combinatorial methods for phylogenetic networks. Genome Biol Evol. 2011, 3: 23-35. 10.1093/gbe/evq077.PubMedPubMed CentralView Article
- Shimodaira H, Hasegawa M: Multiple comparisons of Log-likelihoods with applications to phylogenetic inference. Mol Biol Evol. 1999, 16: 1114-1116. 10.1093/oxfordjournals.molbev.a026201.View Article
- Zink MJ, Ph.D. Dissertation: Systematics of the fern genus Lepisorus (J. Smith) Ching (Polypodiaceae–Lepisoreae). 1993, Zurich: University Zurich
- Dolozel J, Greilhuber J, Suda J: Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc. 2007, 2: 2233-2244. 10.1038/nprot.2007.310.View Article
- Erkt L, Holobova R, Travnicek P, Suda J: Species boundaries and frequency of hybridization in the Dryopteris carthusiana (Dryopteridaceae) complex: a taxonomic puzzle resolved using genome size data. Am J Bot. 2010, 97: 1208-1219. 10.3732/ajb.0900206.View Article
- Loureiro J, Rodriguez E, Dolezel J, Santo C: Two new nuclear isolation buffers for plant DNA cytometry: a test with 37 species. Ann Bot. 2007, 100: 875-888. 10.1093/aob/mcm152.PubMedPubMed CentralView Article
- Tsitrone A, Kirkpatric M, Levin DA: A model for chloroplast capture. Evolution. 2003, 57: 1776-1782.PubMedView Article
- Hedke SM, Hillis DM: The potential role of androgenesis in cytoplasmic-nuclear phylogenetic discordance. Syst Biol. 2011, 60: 87-109. 10.1093/sysbio/syq070.View Article
- Nitta JH, Ebihara A, Ito M: Reticulate evolution in the Crepidomanes minutum species complex (Hymenophyllaceae). Am J Bot. 2011, 98: 1782-1800. 10.3732/ajb.1000484.PubMedView Article
- Ebihara A, Ishikawa H, Matsumoto S, Lin SJ, Iwatsuki K, Takamiya M, Watano Y, Ito M: Nuclear DNA, chloroplast DNA, and ploidy analysis clarified biological complexity of the Vandenboschia radicans complex (Hymenophyllaceae) in Japan and adjacent regions. Am J Bot. 2005, 92: 1535-1547. 10.3732/ajb.92.9.1535.PubMedView Article
- Ebihara A, Matsumoto S, Ito M: Hybridiztion involving independent gametophytes in the Vandenboschia radicans complex (Hymenophyllaceae): a new perspective on the distribution of fern hybrids. Mol Ecol. 2009, 18: 4904-4911. 10.1111/j.1365-294X.2009.04406.x.PubMedView Article
- Schuettpelz E, Grusz AL, Windham MD, Pryer KM: The utility of nuclear gapCp in resolving polyploid fern origins. Syst Bot. 2008, 33: 621-629. 10.1600/036364408786500127.View Article
- Beck JB, Windham MD, Yatskievych G, Pryer KM: A diploid-first approach to species delimitation and interpreting polyploid evolution in the fern genus Astrolepis (Pteridaceae). Syst Biol. 2010, 35: 223-234.
- Beck JB, Windham MD, Pryer KM: Do asexual polyploid lineages led short evolutionary lives? A case study from the fern genus Astrolepis. Evolution. 2011, 65: 327-3229.View Article
- Juslen A, Henry A, Wikstrom N: Relationships and evolutionary origin of polyploid Dryopteris (Dryopteridaceae) from Europe inferred using nuclear pgiC and plastid trnL-F sequence data. Taxon. 2011, 60: 1284-1294.
- Grusz AL, Windham MD, Pryer KM: Deciphering the origins of apomictic polploids in the Cheilanthes yavapensis complex (Pteridaceae). Am J Bot. 2009, 96: 1636-1645. 10.3732/ajb.0900019.PubMedView Article
- Vogel JC, Rumsey FJ, Schneller JJ, Barrett JA, Gibby M: Where are the glacial refugia in Europe? Evidence from pteridophytes. Biol J Linn Soc. 1999, 66: 23-37.View Article
- Hunt HV, Ansell SE, Russell SJ, Schneider H, Vogel JC: Genetic diversity and phlogeography in two diploid ferns, Asplenium fontanum subsp. fontanum and A. petrarchae subsp. bivalens, in the western Mediterranean. Mol Ecol. 2009, 18: 4940-4954. 10.1111/j.1365-294X.2009.04402.x.PubMedView Article
- Medail F, Verlaque R: Ecological characteristics and rarity of endemic plants from southeast France and Corsica: implications for biodiversity conservation. Biol Conserv. 1997, 80: 269-281. 10.1016/S0006-3207(96)00055-9.View Article
- Sun H, Wang AL, Wan DS, Wang Q, Liu JQ: Rapid radiation of Rheum (Polygonaceae) and parallel evolution of morphological traits. Molec Phylogen Evol. 2012, 63: 150-158. 10.1016/j.ympev.2012.01.002.View Article
- Boucher FC, Thuiller W, Roqet C, Douzet R, Aubert S, Alvarez N, Lavergne S: Reconstructing the origins of high-alpine niches and cushion life form in the genus Androsace s.l. (Primulaceae). Evolution. 2012, 66: 1255-1268. 10.1111/j.1558-5646.2011.01483.x.PubMedPubMed CentralView Article
- Liu JQ, Sun YS, Ge XJ, Gao LM, Qiu YX: Phylogeographic studies of plants in China: advances in the past and directions in the future. J Syst Evol. 2012, 50: 267-275. 10.1111/j.1759-6831.2012.00214.x.View Article
- Xu TT, Abbott RJ, Mao K, Du FK, Wu GL, Ciren ZX, Miehe G, Liu JQ: Phylogeography and allopatric divergence of cypress species (Cupressus L.) in the Qinghai-Tibetan Plateau and adjacent regions. BMC Evol Biol. 2010, 10: 194-10.1186/1471-2148-10-194.PubMedPubMed CentralView Article
- Yang FS, Qin A, Wang XQ: Great genetic differentiation among populations of Meconopsis integrifolia and its implication for plant speciation in the Qinghai-Tibetan plateau. PLoS One. 2012, 7: e37196-10.1371/journal.pone.0037196.PubMedPubMed CentralView Article
- Casazza G, Granato L, Minuto L, Conti E: Polyploid evolution and Pleistocene glacial cycles: a case study from the alpine primrosePrimula marginata(Primulaceae). BMC Evol Biol. 2012, 12: 56-10.1186/1471-2148-12-56.PubMedPubMed CentralView Article
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