Postglacial species displacement in Triturus newts deduced from asymmetrically introgressed mitochondrial DNA and ecological niche models
© Wielstra and Arntzen; licensee BioMed Central Ltd. 2012
Received: 1 June 2012
Accepted: 25 August 2012
Published: 30 August 2012
If the geographical displacement of one species by another is accompanied by hybridization, mitochondrial DNA can introgress asymmetrically, from the outcompeted species into the invading species, over a large area. We explore this phenomenon using the two parapatric crested newt species, Triturus macedonicus and T. karelinii, distributed on the Balkan Peninsula in south-eastern Europe, as a model.
We first delimit a ca. 54,000 km2 area in which T. macedonicus contains T. karelinii mitochondrial DNA. This introgression zone bisects the range of T. karelinii, cutting off a T. karelinii enclave. The high similarity of introgressed mitochondrial DNA haplotypes with those found in T. karelinii suggests a recent transfer across the species boundary. We then use ecological niche modeling to explore habitat suitability of the location of the present day introgression zone under current, mid-Holocene and Last Glacial Maximum conditions. This area was inhospitable during the Last Glacial Maximum for both species, but would have been habitable at the mid-Holocene. Since the mid-Holocene, habitat suitability generally increased for T. macedonicus, whereas it decreased for T. karelinii.
The presence of a T. karelinii enclave suggests that T. karelinii was the first to colonize the area where the present day introgression zone is positioned after the Last Glacial Maximum. Subsequently, we propose T. karelinii was outcompeted by T. macedonicus, which captured T. karelinii mitochondrial DNA via introgressive hybridization in the process. Ecological niche modeling suggests that this replacement was likely facilitated by a shift in climate since the mid-Holocene. We suggest that the northwestern part of the current introgression zone was probably never inhabited by T. karelinii itself, and that T. karelinii mitochondrial DNA spread there through T. macedonicus exclusively. Considering the spatial distribution of the introgressed mitochondrial DNA and the signal derived from ecological niche modeling, we do not favor the hypothesis that foreign mitochondrial DNA was pulled into the T. macedonicus range by natural selection.
KeywordsContact zone Ecological niche modeling Historical biogeography Phylogeography
Parapatric species can show geographically asymmetric introgression, with one species showing genes typical of the other over a significant part of its range [reviewed in ref. . This could be caused by natural selection, pulling favorable foreign genes into a species’ range . However, empirical and simulation studies show that if displacement of one species by another coincides with hybridization, introgression of neutral genes from the native to the invading species is the expected outcome [4, 5]. Furthermore, markers showing reduced intraspecific gene flow (such as mitochondrial DNA) are particularly susceptible to introgression, as their dispersal limitation reduces the chances of being genetically swamped [4, 5].
We explain the principle of introgression due to species displacement using mitochondrial DNA as an example (Figure 1b). The rationale is as follows: mitochondrial DNA of a common, native ‘donor’ species is captured by an initially rare invader, through introgressive hybridization. As the invader expands its range at the expense of the native species, its population increases and the introgressed mitochondrial DNA is co-amplified. Consequently, the introgressed mitochondrial DNA surfs the wave of advance and is left in its wake. Initial introgression does not have to occur at a high frequency to result in a mismatch between species identity and mitochondrial DNA type spanning an extensive area.
The climate oscillations related to the glacial/interglacial cycles of the Quaternary ice age repeatedly forced species to shift their distributions . Distribution rearrangement of closely related species after postglacial secondary contact is considered a likely driver of introgression [3, 4]. To explore the role of postglacial displacement in introgression, ecological niche modeling - the approximation of the ecological requirements of a species based on the range of environmental conditions experienced at known localities  - can be used. By extrapolating ecological niche models on both current and past climate layers, distribution shifts can be identified . By determining which part of the current distribution was uninhabitable at the Last Glacial Maximum (~21 Ka), the area colonized post-glacially can be approximated. Introgression of neutral genes after species displacement would likely be restricted to this particular region, whereas such a geographical limitation would not apply to introgression by positive selection.
Model system: Triturus newts
Triturus macedonicus and T. karelinii are not sister groups: their most recent common ancestor is the ancestor of all crested newt species (the T. cristatus superspecies), estimated to have lived c. 10–11 million years ago [13, 15, 16]. Analysis of a suite of allozyme markers has shown the nuclear gene pools of T. macedonicus and T. karelinii to have remained isolated [17, 18], Arntzen et al. submitted]. The mitochondrial DNA of the two species is highly distinct . Furthermore, T. macedonicus and T. karelinii can be distinguished by morphology, including meristic, morphometric and qualitative characters [14, 19].
Limited admixture of the nuclear genome does occur at syntopic populations at the contact zone of T. macedonicus and T. karelinii [Arntzen et al. submitted] (Figure 2a). Furthermore, geographical displacement by T. macedonicus has been invoked to explain an apparent enclave of T. karelinii, cut off from the main distribution range . These factors would facilitate asymmetric introgression. Indeed, mitochondrial DNA typical of T. karelinii has been found in T. macedonicus, at localities removed from the current contact zone [20, 21]. Here we further explore the introgression of T. karelinii mitochondrial DNA into T. macedonicus. We first delimit the zone of T. karelinii mitochondrial DNA asymmetrically introgressed into T. macedonicus through a dense phylogeographic survey. We then build ecological niche models for both species and project these on current, mid-Holocene (~6 Ka) and Last Glacial Maximum (~21 Ka) climate layers. Inferred distribution shifts across the current zone of asymmetric mitochondrial DNA introgression suggest post-glacial displacement of T. karelinii by T. macedonicus.
As T. macedonicus and T. karelinii mitochondrial DNA differs considerably (DXY = 0.091), sequenced individuals could be unambiguously assigned to mitochondrial DNA type (details in Additional file 1). Out of 71 of the T. macedonicus localities for which sequence data are available, 35 have only original T. macedonicus mitochondrial DNA, 34 have only T. karelinii mitochondrial DNA and two localities show syntopy of both mitochondrial DNA types (Figure 2a). There is no T. macedonicus mitochondrial DNA found in any of the 86 T. karelinii localities for which sequence data is available. Based on the known geographical distribution of mitochondrial DNA we interpret mitochondrial DNA type for the remaining localities for which sequence data are not available (Figure 2a). By employing Thiessen polygons, we delimit an extensive (ca. 54,000 km2) area of asymmetric mitochondrial DNA introgression, in which T. macedonicus contains T. karelinii mitochondrial DNA (Figure 2b).
A scenario of species displacement
We confirm that the range of T. karelinii is bisected by T. macedonicus (Figure 2a), corroborating a previously hypothesized T. karelinii enclave . Considering that crested newts are surface-bound and have limited dispersal capabilities, the presence of the T. karelinii enclave is best explained by it having been cut off from the main T. karelinii range by expansion of T. macedonicus. However, the mitochondrial DNA data suggest that a considerably larger area is involved in the geographical displacement of T. karelinii by T. macedonicus than that required to explain the enclave per se (Figure 2b). How was this introgression zone established? The structuring of the mitochondrial DNA of the two crested newt species and predicted distributions based on ecological niche modeling provide useful insight.
The ranges of both crested newt species were reduced at the Last Glacial Maximum (Figure 5). For T. karelinii, the southwestern margin of its current Balkan range (extending into land currently occupied by T. macedonicus) is predicted to have been hospitable. However, the mitochondrial DNA data suggest that the ‘Balkan ancestral’ clade was likely restricted to the south-east, whereas the ‘Balkan derived’ clade originates from a recent colonization from Asiatic Turkey (Figure 3). Indeed, Turkey’s west coast was also predicted suitable at the Last Glacial Maximum and mitochondrial DNA indicates a stable demographical history here (Figure 5). Furthermore, the ‘Balkan derived’ clade shows the signature of demographic growth, in line with a range expansion (Figure 4). The west side of the current range of T. macedonicus was also predicted to have been suitable at the Last Glacial Maximum, corresponding to long-lasting presence in situ as suggested by the mitochondrial DNA data (Figures 4 and 5).
The area where the introgression zone is situated was uninhabitable for both crested newt species at the Last Glacial Maximum and secondary contact between the two was established after glacial conditions had alleviated (Figure 5). Furthermore, the T. karelinii haplotypes introgressed into T. macedonicus are similar or identical to those found in T. karelinii itself. Taken together, this suggests that introgression happened after the Last Glacial Maximum. We did not detect more ancient mitochondrial DNA introgression (as found in other taxa by e.g. [22–25]). Given the climatic oscillations during the Quaternary Ice Age, the two newt species are presumed to have been in periodic contact through time during previous interglacials. However, as subsequent glacial periods would cause their ranges to recede again, any mitochondrial DNA that became introgressed in the area outside of their glacial refugia would be erased.
The climate changed considerably between the Last Glacial Maximum and the present, but we have an intervening snapshot at the mid-Holocene. Compared to the mid-Holocene, the suitability of the location of the present day introgression zone generally increased for T. macedonicus, whereas it decreased for T. karelinii (Figure 5). Life history differences between the two species provide some suggestion of how T. macedonicus was subsequently able to outcompete T. karelinii, under the changing abiotic environment. The two crested newt species appear to differ in the time annually allocated to an aquatic and a terrestrial life style, with adult T. macedonicus presumed to spend one more month in the water than T. karelinii[13, 14]. This suggests that T. macedonicus is adapted to relatively wetter conditions. Bioclimatic variable contribution to the ecological niche models is in line with this hypothesis: the occurrence of T. macedonicus correlates with a higher precipitation than that of T. karelinii (Additional file 5). The climate layers used in the environmental niche modeling provide further insight: since the mid-Holocene, precipitation seasonality in the area where the introgression zone is positioned decreased; whereas precipitation during the wettest season decreased, it increased during the driest season (Additional file 6). Further research is required to understand the adaptive advantage of T. macedonicus over T. karelinii in the introgression zone.
Reinforcement of asymmetric mitochondrial DNA transmission
Geographical species displacement involves a demographical inequality: at the leading edge, the invader is rare whereas the species being displaced is common. This demographic inequality is in itself sufficient to explain asymmetrical mitochondrial DNA introgression . However, the pattern can be reinforced by male biased dispersal and through the operation of Haldane’s rule . Males dispersing into territory occupied by a species with which they can hybridize would only encounter females of the indigenous species to mate with. Most of their genetic material can potentially be transmitted to future generations, but their offspring would always contain indigenous mitochondrial DNA, as this is transmitted matrilinearily only. Whether there is male-biased dispersal in crested newts is not known. Haldane’s rule  states that if one sex is absent, rare, or sterile in F1 offspring, that sex is the heterogametic sex. In the case of crested newts, this would be the male . In effect, markers transmitted via the female line (i.e. mitochondrial DNA) would be particularly prone to introgress . Male biased dispersal and Haldane’s rule could act in combination while assortative mating would further amplify asymmetric mitochondrial DNA introgression : if members of the native species prefer to mate among themselves, the invading males and the excess of hybrid females would be more likely to mate with each other.
Even when the parental species occurs in the same frequency, an asymmetry in mitochondrial DNA transmission could be strengthened by factors favoring the relative frequency (prezygotic factors) or viability (postzygotic factors) of one hybrid class over the other [2–4]. Examples of pre- and postzygotic selection are known in nature. In the tree frogs Hyla cinerea and H. gratiosa, differences in behavior (courting at the edge versus inside the breeding pond and presence versus absence of satellite mating behavior) cause H. cinerea males to more often intercept H. gratiosa females than vice versa, resulting in biased mitochondrial DNA transmission . For the crested newt T. cristatus and the marbled newt T. marmoratus, F1 hybrids make up about four percent of the adult population where the ranges of the two species overlap . Uneven viability of hybrids causes the frequency of T. marmoratus-mothered hybrids to drop from fifty percent in embryos to ten percent in adults. The T. cristatus-mothered hybrids show an excess of females (i.e. a Haldane’s effect). However, the T. marmoratus-mothered hybrids consist of males only, suggesting an incompatibility between the T. cristatus X chromosome and T. marmoratus cytoplasm .
In the present case, a higher frequency or reproductive success of T. karelinii-mothered hybrids over T. macedonicus-mothered hybrids would have acted like a filter, hampering the spread of T. macedonicus mitochondrial DNA into the introgression zone. Research into the presence of such asymmetries in T. macedonicus – T. karelinii hybridization is a prospect for future study.
What about positive selection?
Asymmetric mitochondrial DNA introgression due to the positive selection of foreign mitochondrial DNA [6, 8, 31] is characterized by a distinctly different pattern of geographical spread than if caused by species displacement: instead of the contact zone between two species moving across the initial mitochondrial DNA boundary, which is the case with geographical displacement (Figure 1b), positive selection results in mitochondrial DNA of one species being ‘pulled’ into the range of the other. A selective sweep, where foreign mitochondrial DNA replaces the original mitochondrial DNA, has been demonstrated experimentally in Drosophila flies . Positive selection has also been suggested to cause asymmetric mitochondrial DNA introgression in nature [33, 34]. However, when encountering asymmetric mitochondrial DNA introgression, it is difficult to choose between a scenario of species displacement or natural selection. For example, in Lissotriton newts the original mitochondrial DNA of L. montandoni has been fully replaced by that of L. vulgaris. However, this exchange of mitochondrial DNA did not have a single origin but involved multiple, independent introgression events of well-differentiated L. vulgaris mitochondrial DNA. The observed pattern could either reflect repeated geographical replacement during multiple interglacials, when the more mountainous L. montandoni shifted its range to a lower elevation, or several uptakes of foreign mitochondrial DNA that subsequently spread in L. montandoni by natural selection.
Why do we in the present case favor the geographical displacement scenario over the positive selection scenario? The bisecting of the T. karelinii range by T. macedonicus provides positive evidence in favor of T. macedonicus expanding its range at the expense of T. karelinii. This displacement is further supported by T. karelinii mitochondrial DNA having spread into a region where we infer T. karelinii was never present – this would require a T. macedonicus host that was expanding its range after having captured T. karelinii mitochondrial DNA. Furthermore, foreign T. karelinii mitochondrial DNA is only present in T. macedonicus in postglacially colonized areas. If positive selection had caused the asymmetric mitochondrial DNA introgression, foreign mitochondrial could also have penetrated into the region that acted as a glacial refugium for T. macedonicus. Only if an adaptive advantage of foreign mitochondrial DNA was somehow limited to the area where the introgression zone is currently positioned, it would be indistinguishable from a scenario involving species displacement. However, we know that T. macedonicus has at least partially displaced T. karelinii, based on the well-supported T. karelinii enclave. Considering that species displacement occurred at least in part of the introgression zone, the most parsimonious explanation is that species displacement alone caused the pattern of asymmetric mitochondrial DNA introgression observed, instead of a combination of species displacement and localized positive selection of mitochondrial DNA. To test this, future research should employ a large battery of nuclear DNA sequences. Selectively neutral nuclear genes would be expected to show a comparable geographical pattern of introgression compared to mitochondrial DNA when asymmetrical mitochondrial DNA introgression was caused by species displacement . This would, however, not be the expectation in the case of positive selection of mitochondrial DNA.
The T. macedonicus – T. karelinii case provides a unique insight into asymmetric mitochondrial DNA introgression. The fact that T. macedonicus bisects the range of T. karelinii provides a clear indication of directionality: T. macedonicus likely invaded T. karelinii territory instead of the other way around. Based on a combination of phylogeography and ecological niche modeling, we suggest that the asymmetric introgression of mitochondrial DNA from T. karelinii into T. macedonicus is due to postglacial species displacement (see the summarized scenario in Figure 6). As these crested newt species expanded their ranges in response to climate change after the conclusion of the Last Glacial Maximum, they came into spatial contact. Subsequently, the newly established hybrid zone between the two moved across the landscape, as T. macedonicus outcompeted T. karelinii. However, the former distribution of T. karelinii, before T. macedonicus started to invade its range, could be inferred based on the asymmetrically introgressed T. karelinii mitochondrial DNA. The asymmetrical introgression of mitochondrial DNA has been regularly documented in a wide range of taxa and can provide key insights into historical biogeography [4, 36].
Distribution and genetic data
We included 139 T. macedonicus and 135 T. karelinii localities (Figure 2 and Additional file 1). Species identity was primarily based on morphological characters (throat and belly pattern and morphological measurements); for a subset (conveniently arranged along transects across the contact zone) diagnostic allozyme markers have been published [17, 18], Arntzen et al. submitted]. Note that at five localities the species are found in syntopy. We obtained a 658-bp segment of ND4 for 600 newts (70 previously published in, and 530 newly sequenced following the protocol of ) from 71 of the T. macedonicus and 86 of the T. karelinii localities (Figure 2 and Additional file 1). Among them, the sequenced individuals contained 83 haplotypes (Additional file 3). We delimited the zone of asymmetrically introgressed mitochondrial DNA by Thiessen polygons, using ArcGIS (http://www.esri.com). Each polygon represents a locality and contains the area that is closer to that particular locality than to another one [as in .
Phylogenetic trees were constructed with MrBayes 3.1.2 , employing two, four-chain, twenty million generation runs, with a sampling frequency of 0.001 and a heating parameter of 0.1. MrModeltest  identified GTR + I, HKY + I + G and HKY + G as the most suitable models of sequence evolution for codon positions 1, 2 and 3. Data partitions were unlinked. Tracer 1.5  was used to check for stabilization of overall likelihood within and convergence between runs. The first quarter of the sampled trees was discarded as burn-in. As an outgroup we used T. marmoratus (GenBank accession number GU982379). Minimum spanning haplotype networks were created with HapStar 0.5 , based on distance matrices produced with Arlequin 3.5 . DnaSP5  was used to determine the nucleotide diversity (π) within and the average number of nucleotide substitutions (DXY) between groups of haplotypes. To test for signals of demographic expansion, we compared the observed mismatch distribution with that expected under population expansion . We interpreted the differences based on the Ramos-Onsins and Rozas’ R 2 and Fu’s Fs statistics , for which statistical significance was obtained with a 1,000 coalescent simulations in DNAsp, and based on the sum of square deviations, for which statistical was evaluated with a 1,000 bootstrap replicates, in Arlequin 3.5 .
For climate layers we used bioclimatic variables at 2.5 arcminute resolution, available from WorldClim 1.4 . To obtain realistic and transferable ecological niche models, it is recommended to mirror the physiological limitations of the study species and minimize the effects of multi-colinearity among data layers [47–50]. Crested newt species differ in the length of their annual aquatic period [13, 14]. Therefore, we include a set of layers that likely reflects the availability of water bodies during the breeding season, i.e. seasonal variation in evaporation and precipitation: bio10 = mean temperature of warmest quarter, bio11 = mean temperature of coldest quarter, bio15 = precipitation seasonality, bio16 = precipitation of wettest quarter, and bio17 = precipitation of driest quarter. These layers show a Pearson correlation < 0.7. Bioclimatic variables are also available for the mid-Holocene (~6 Ka) and the Last Glacial Maximum (~21 Ka), being derived from the Paleoclimate Modelling Intercomparison Project phase 2 ; http://pmip2.lsce.ipsl.fr/. We used datalayers for the former based on simulations using the ECHAM3 model (Model Max Planck Inst. für Meteorologie) and the latter based on simulations using the Community Climate System Model version 3 (CCSM) .
Ecological niche modeling
Ecological niche models were created using Maxent 3.3.3e . We restricted the feature type to hinge features as this produces smoother model fits, so forcing models to be more focused on key trends rather than potential idiosyncrasy in the data, which would hamper extrapolation to a different time or place . We restricted the area from which pseudo-absence was drawn to the distribution of the entire crested newt T. cristatus superspecies. This area was broadly defined as a 200 km buffer zone  around known crested newt localities [Wielstra et al., submitted]. The ecological niche models were tested for statistical significance against a null model derived from random localities, to test whether they perform better than expected by random chance . We created a null distribution of 99 AUC values, based on as many random localities as used for the tested species distribution model. The AUC value of the tested species distribution model was treated as a 100th value and deemed statistically significant if it ranked higher than the 95th value (i.e. above the 95% confidence interval). Random point data were created with ENMTools 1.3 ; http://enmtools.blogspot.com/. The null model approach prevents interpreting model quality based on an arbitrary AUC threshold and precludes the requirement to set aside part of the localities for model testing . Models were projected on the current and Last Glacial Maximum climate layers. We explored variable importance and response curves and conducted a jackknife test to determine how ecological niche models of the two species differed.
W. Babik, W. Beukema, A.K. Skidmore, A.G. Toxopeus and G.P. Wallis provided comments on an earlier version of this paper. The PMIP 2 Data Archive is supported by CEA, CNRS and the Programme National d'Etude de la Dynamique du Climat (PNEDC). We acknowledge the international modeling groups for providing their data for analysis and the Laboratoire des Sciences du Climat et de l'Environnement (LSCE) for collecting and archiving the model data.
- Coyne JA, Orr HA: Speciation. 2004, Sinauer Associates, Sunderland
- Chan KMA, Levin SA: Leaky prezygotic isolation and porous genomes: Rapid introgression of maternally inherited DNA. Evolution. 2005, 59 (4): 720-729.PubMedView Article
- Excoffier L, Foll M, Petit RJ: Genetic consequences of range expansions. Annu Rev Ecol Evol Syst. 2009, 40: 481-501. 10.1146/annurev.ecolsys.39.110707.173414.View Article
- Currat M, Ruedi M, Petit RJ, Excoffier L: The hidden side of invasions: Massive introgression by local genes. Evolution. 2008, 62 (8): 1908-1920.PubMed
- Petit RJ, Excoffier L: Gene flow and species delimitation. Trends Ecol Evol. 2009, 24 (7): 386-393. 10.1016/j.tree.2009.02.011.PubMedView Article
- Galtier N, Nabholz B, Glemin S, Hurst GDD: Mitochondrial DNA as a marker of molecular diversity: A reappraisal. Mol Ecol. 2009, 18 (22): 4541-4550. 10.1111/j.1365-294X.2009.04380.x.PubMedView Article
- Gyllensten U, Wharton D, Wilson AC: Maternal inheritance of mitochondrial DNA during backcrossing of two species of mice. J Hered. 1985, 76 (5): 321-324.PubMed
- Ballard JWO, Whitlock MC: The incomplete natural history of mitochondria. Mol Ecol. 2004, 13 (4): 729-744. 10.1046/j.1365-294X.2003.02063.x.PubMedView Article
- Hewitt G: The genetic legacy of the Quaternary ice ages. Nature. 2000, 405 (6789): 907-913. 10.1038/35016000.PubMedView Article
- Kozak KH, Graham CH, Wiens JJ: Integrating GIS-based environmental data into evolutionary biology. Trends Ecol Evol. 2008, 23 (3): 141-148. 10.1016/j.tree.2008.02.001.PubMedView Article
- Svenning J-C, Fløjgaard C, Marske KA, Nógues-Bravo D, Normand S: Applications of species distribution modeling to paleobiology. Quaternary Sci Rev. 2011, 30 (21–22): 2930-2947.View Article
- Wielstra B, Espregueira Themudo G, Güclü Ö, Olgun K, Poyarkov NA, Arntzen JW: Cryptic crested newt diversity at the Eurasian transition: The mitochondrial DNA phylogeography of Near Eastern Triturus newts. Mol Phylogenet Evol. 2010, 56 (3): 888-896. 10.1016/j.ympev.2010.04.030.PubMedView Article
- Wielstra B, Arntzen JW: Unraveling the rapid radiation of crested newts (Triturus cristatus superspecies) using complete mitogenomic sequences. BMC Evol Biol. 2011, 11: 162-10.1186/1471-2148-11-162.PubMedPubMed CentralView Article
- Arntzen JW: Triturus cristatus Superspecies - Kammolch-Artenkreis (Triturus cristatus (Laurenti, 1768) - Nördlicher Kammolch, Triturus carnifex (Laurenti, 1768) - Italienischer Kammolch, Triturus dobrogicus (Kiritzescu, 1903) - Donau-Kammolch, Triturus karelinii (Strauch, 1870) - Südlicher Kammolch). Handbuch der Reptilien und Amphibien Europas Schwanzlurche IIA. Edited by: Grossenbacher K, Thiesmeier B. 2003, Aula-Verlag, Wiebelsheim, 421-514.
- Espregueira Themudo G, Wielstra B, Arntzen JW: Multiple nuclear and mitochondrial genes resolve the branching order of a rapid radiation of crested newts (Triturus, Salamandridae). Mol Phylogenet Evol. 2009, 52 (2): 321-328. 10.1016/j.ympev.2009.03.024.PubMedView Article
- Wiens JJ, Sparreboom M, Arntzen JW: Crest evolution in newts: Implications for reconstruction methods, sexual selection, phenotypic plasticity and the origin of novelties. J Evol Biol. 2011, 24 (10): 2073-2086. 10.1111/j.1420-9101.2011.02340.x.PubMedView Article
- Arntzen JW, Wielstra B: Where to draw the line? A nuclear genetic perspective on proposed range boundaries of the crested newts Triturus karelinii and T. arntzeni. Amphibia-Reptilia. 2010, 31 (3): 311-322. 10.1163/156853810791769509.View Article
- Arntzen JW: Genetic variation in the Italian crested newt, Triturus carnifex, and the origin of a non-native population north of the Alps. Biodivers Conserv. 2001, 10 (6): 971-987. 10.1023/A:1016644814551.View Article
- Arntzen JW, Wallis GP: Geographic variation and taxonomy of crested newts (Triturus cristatus superspecies): Morphological and mitochondrial data. Contrib Zool. 1999, 68 (3): 181-203.
- Wallis GP, Arntzen JW: Mitochondrial-DNA variation in the crested newt superspecies: Limited cytoplasmic gene flow among species. Evolution. 1989, 43 (1): 88-104. 10.2307/2409166.View Article
- Arntzen JW, Espregueira Themudo G, Wielstra B: The phylogeny of crested newts (Triturus cristatus superspecies): Nuclear and mitochondrial genetic characters suggest a hard polytomy, in line with the paleogeography of the centre of origin. Contrib Zool. 2007, 76 (4): 261-278.
- McGuire JA, Linkem CW, Koo MS, Hutchison DW, Lappin AK, Orange DI, Lemos-Espinal J, Riddle BR, Jaeger JR: Mitochondrial introgression and incomplete lineage sorting through space and time: Phylogenetics of crotaphytid lizards. Evolution. 2007, 61 (12): 2879-2897. 10.1111/j.1558-5646.2007.00239.x.PubMedView Article
- Bryson JRW, De Oca AN-M, Jaeger JR, Riddle BR: Elucidation of cryptic diversity in a widespread Nearctic treefrog reveals episodes of mitochondrial gene capture as frogs diversified across a dynamic landscape. Evolution. 2010, 64 (8): 2315-2330.PubMed
- Bossu CM, Near TJ: Gene trees reveal repeated instances of mitochondrial DNA introgression in orangethroat darters (Percidae: Etheostoma). Syst Biol. 2009, 58 (1): 114-129. 10.1093/sysbio/syp014.PubMedView Article
- Liu K, Wang F, Chen W, Tu L, Min M-S, Bi K, Fu J: Rampant historical mitochondrial genome introgression between two species of green pond frogs, Pelophylax nigromaculatus and P. plancyi. BMC Evol Biol. 2010, 10 (1): 201-10.1186/1471-2148-10-201.PubMedPubMed CentralView Article
- Beysard M, Perrin N, Jaarola M, Heckel G, Vogel P: Asymmetric and differential gene introgression at a contact zone between two highly divergent lineages of field voles (Microtus agrestis). J Evol Biol. 2012, 25 (2): 400-408. 10.1111/j.1420-9101.2011.02432.x.PubMedView Article
- Haldane JBS: Sex ratio and unisexual sterility in hybrid animals. J Genet. 1922, 12 (2): 101-109. 10.1007/BF02983075.View Article
- Macgregor HC: An introduction to animal cytogenetics. 1993, Chapman & Hall, London
- Lamb T, Avise JC: Directional introgression of mitochondrial DNA in a hybrid population of tree frogs: The influence of mating behavior. Proc Natl Acad Sci USA. 1986, 83 (8): 2526-2530. 10.1073/pnas.83.8.2526.PubMedPubMed CentralView Article
- Arntzen JW, Jehle R, Bardakci F, Burke T, Wallis GP: Asymmetric viability of reciprocal-cross hybrids between crested and marbled newts (Triturus cristatus and T. marmoratus). Evolution. 2009, 63 (5): 1191-1202. 10.1111/j.1558-5646.2009.00611.x.PubMedView Article
- Rand DM: The units of selection on mitochondrial DNA. Annu Rev Ecol Syst. 2001, 32: 415-448. 10.1146/annurev.ecolsys.32.081501.114109.View Article
- Niki Y, Chigusa SI, Matsuura ET: Complete replacement of mitochondrial DNA in Drosophila. Nature. 1989, 341 (6242): 551-552. 10.1038/341551a0.PubMedView Article
- Irwin DE, Rubtsov AS, Panov EN: Mitochondrial introgression and replacement between yellowhammers (Emberiza citrinella) and pine buntings (Emberiza leucocephalos) (Aves: Passeriformes). Biol J Linn Soc. 2009, 98 (2): 422-438. 10.1111/j.1095-8312.2009.01282.x.View Article
- Plötner J, Uzzell T, Beerli P, Spolsky C, Ohst T, Litvinchuk SN, Guex GD, Reyer HU, Hotz H: Widespread unidirectional transfer of mitochondrial DNA: a case in western Palaearctic water frogs. J Evol Biol. 2008, 21 (3): 668-681. 10.1111/j.1420-9101.2008.01527.x.PubMedPubMed CentralView Article
- Babik W, Branicki W, Crnobrnja-Isailovic J, Cogalniceanu D, Sas I, Olgun K, Poyarkov NA, Garcia-Paris M, Arntzen JW: Phylogeography of two European newt species - Discordance between mtDNA and morphology. Mol Ecol. 2005, 14 (8): 2475-2491. 10.1111/j.1365-294X.2005.02605.x.PubMedView Article
- Toews DPL, Brelsford A: The biogeography of mitochondrial and nuclear discordance in animals. Mol Ecol. 2012, 21 (16): 3907-3930. 10.1111/j.1365-294X.2012.05664.x.PubMedView Article
- Arntzen JW, Wallis GP: Restricted gene flow in a moving hybrid zone of the newts Triturus cristatus and T. marmoratus in western France. Evolution. 1991, 45 (4): 805-826. 10.2307/2409691.View Article
- Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19 (12): 1572-1574. 10.1093/bioinformatics/btg180.PubMedView Article
- Nylander JAA: MrModelTest2. Computer program distributed by the author. Evolutionary Biology Centre. 2004, Uppsala University, Sweden
- Rambaut A, Drummond AJ: Tracer v1.4. 2007, , , http://beast.bio.ed.ac.uk/Tracer,
- Teacher AGF, Griffiths DJ: HapStar: Automated haplotype network layout and visualization. Mol Ecol Resour. 2011, 11 (1): 151-153. 10.1111/j.1755-0998.2010.02890.x.PubMedView Article
- Excoffier L, Lischer HEL: Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour. 2010, 10 (3): 564-567. 10.1111/j.1755-0998.2010.02847.x.PubMedView Article
- Librado P, Rozas J: DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009, 25 (11): 1451-1452. 10.1093/bioinformatics/btp187.PubMedView Article
- Rogers AR, Harpending H: Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol. 1992, 9 (3): 552-569.PubMed
- Ramos-Onsins SE, Rozas J: Statistical properties of bew beutrality tests against population growth. Mol Biol Evol. 2002, 19 (12): 2092-2100. 10.1093/oxfordjournals.molbev.a004034.PubMedView Article
- Hijmans RJ, Cameron SE, Parra JL, Jones PG, Jarvis A: Very high resolution interpolated climate surfaces for global land areas. Int J Climatol. 2005, 25 (15): 1965-1978. 10.1002/joc.1276.View Article
- Rödder D, Schmidtlein S, Veith M, Lötters S: Alien invasive slider turtle in unpredicted habitat: A matter of niche shift or of predictors studied?. PLoS One. 2009, 4 (11): e7843-10.1371/journal.pone.0007843.PubMedPubMed CentralView Article
- Austin M: Species distribution models and ecological theory: A critical assessment and some possible new approaches. Ecol Model. 2007, 200 (1–2): 1-19.View Article
- Guisan A, Thuiller W: Predicting species distribution: Offering more than simple habitat models. Ecol Lett. 2005, 8 (9): 993-1009. 10.1111/j.1461-0248.2005.00792.x.View Article
- Peterson AT: Ecological niche conservatism: a time-structured review of evidence. J Biogeogr. 2011, 38 (5): 817-827. 10.1111/j.1365-2699.2010.02456.x.View Article
- Braconnot P, Otto-Bliesner B, Harrison S, Joussaume S, Peterchmitt JY, Abe-Ouchi A, Crucifix M, Driesschaert E, Fichefet T, Hewitt CD, et al: Results of PMIP2 coupled simulations of the Mid-Holocene and Last Glacial Maximum - Part 1: experiments and large-scale features. Clim Past. 2007, 3 (2): 261-277. 10.5194/cp-3-261-2007.View Article
- Collins WD, Bitz CM, Blackmon ML, Bonan GB, Bretherton CS, Carton JA, Chang P, Doney SC, Hack JJ, Henderson TB, et al: The Community Climate System Model Version 3 (CCSM3). J Climate. 2006, 19 (11): 2122-2143. 10.1175/JCLI3761.1.View Article
- Phillips SJ, Anderson RP, Schapire RE: Maximum entropy modeling of species geographic distributions. Ecol Model. 2006, 190 (3–4): 231-259.View Article
- Elith J, Kearney M, Phillips S: The art of modelling range-shifting species. Methods Ecol Evol. 2010, 1 (4): 330-342. 10.1111/j.2041-210X.2010.00036.x.View Article
- VanDerWal J, Shoo LP, Graham C, Williams SE: Selecting pseudo-absence data for presence-only distribution modeling: How far should you stray from what you know?. Ecol Model. 2009, 220 (4): 589-594. 10.1016/j.ecolmodel.2008.11.010.View Article
- Raes N, ter Steege H: A null-model for significance testing of presence-only species distribution models. Ecography. 2007, 30 (5): 727-736. 10.1111/j.2007.0906-7590.05041.x.View Article
- Warren DL, Glor RE, Turelli M: ENMTools: A toolbox for comparative studies of environmental niche models. Ecography. 2010, 33 (3): 607-611.
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