Horizontally transferred genes in the genome of Pacific white shrimp, Litopenaeus vannamei
© Yuan et al.; licensee BioMed Central Ltd. 2013
Received: 27 February 2013
Accepted: 2 August 2013
Published: 6 August 2013
In recent years, as the development of next-generation sequencing technology, a growing number of genes have been reported as being horizontally transferred from prokaryotes to eukaryotes, most of them involving arthropods. As a member of the phylum Arthropoda, the Pacific white shrimp Litopenaeus vannamei has to adapt to the complex water environments with various symbiotic or parasitic microorganisms, which provide a platform for horizontal gene transfer (HGT).
In this study, we analyzed the genome-wide HGT events in L. vannamei. Through homology search and phylogenetic analysis, followed by experimental PCR confirmation, 14 genes with HGT event were identified: 12 of them were transferred from bacteria and two from fungi. Structure analysis of these genes showed that the introns of the two fungi-originated genes were substituted by shrimp DNA fragment, two genes transferred from bacteria had shrimp specific introns inserted in them. Furthermore, around other three bacteria-originated genes, there were three large DNA segments inserted into the shrimp genome. One segment was a transposon that fully transferred, and the other two segments contained only coding regions of bacteria. Functional prediction of these 14 genes showed that 6 of them might be related to energy metabolism, and 4 others related to defense of the organism.
HGT events from bacteria or fungi were happened in the genome of L. vannamei, and these horizontally transferred genes can be transcribed in shrimp. This is the first time to report the existence of horizontally transferred genes in shrimp. Importantly, most of these genes are exposed to a negative selection pressure and appeared to be functional.
KeywordsHorizontal gene transfer Litopenaeus vannamei Shrimp Bacteria Fungi
Unlike vertical transfer, horizontal gene transfer (HGT) refers to the transfer of genes between organisms and allows recipients to acquire novel traits from the donors . In most cases, horizontally transferred genes (HGT genes) are nonfunctional. However, functional HGTs often benefit the recipients in terms of adaptation to certain specialized niches and are thought to be an important evolutionary impetus . Nowadays, most detected HGT events occur among bacteria inhabiting the same environment, and are a major source of genetic variation in bacteria [3, 4]. However, HGT is also increasingly acknowledged to play an important role in animals . Predominantly prokaryotes-to-animal HGT events have been described between prokaryotes and arthropods or nematodes, and an endosymbiont is the most probable donor for gene transfer because of the close and constant proximity of the cells from both organisms [1, 5–9]. HGT in animals causes divergence in genetic materials and may result in physiological metabolism improvements and the gain of some other phenotypes, e.g. virulence gene transfer can cause emergence of new disease, while HGT genes in aphids can help it produce carotenoid [10–13]. Furthermore, functional HGT genes in animals have been identified play a vital role in the evolution of recipients [1, 2].
The Pacific white shrimp, Litopenaeus vannamei, is an economically important marine aquaculture species worldwide. However, the development of the commercial culture of shrimps has generally been accompanied by increasing problems with diseases, which are mostly caused by opportunistic pathogens, such as viruses, bacteria and fungi . Erosions of the cuticle, localized lesions and generalized septicemias are the three general symptoms seen in infected shrimps . Generally, shrimps living in complex environments with various parasitic or symbiotic microorganisms, and the communications between them always make effects on their growth [15–18]. The close relationship between shrimps and their parasitic or symbiotic organisms may provide a platform for trans-kingdom horizontal transfer of genetic materials during evolutionary history [1, 3]. However, no strong evidence for the HGT events in shrimp has been found.
The detection of animal HGT events has been based on complete genome sequences and the combination of homology searching and phylogenetic analysis . Unfortunately, the whole genome and complete gene set of shrimp have not been published. However, recently, a large number of expressed sequences tags (ESTs) and high-throughput transcriptome sequencing data of L. vannamei were published [20, 21]. These data provide good resources for the exhaustive HGTs detection in shrimp. In this study, the candidate HGT genes in the genome of L. vannamei were searched by sequence homology comparison, phylogenetic analysis, and experimental confirmation of the candidates HGT genes. By comparing the HGT genes-located shrimp genome contigs and corresponding donor genomes, the relatively large genomic segments, which included gene clusters, have been identified as being horizontally transferred from bacteria and integrated into the shrimp genome. Furthermore, their expression profiles at five developmental stages of shrimp were analyzed and their probable functions in shrimp were discussed.
Fourteen HGT genes were detected in L. vannamei
Three kinds of phyloenetic analysis were performed on the remaining 722 HGT candidates after homology search (see Methods). Phylogenetic trees for each candidate HGT gene were constructed manually, and once a gene was nested within a donor clade and many other donor species formed basal branches, it was considered to be a HGT gene that had horizontally transferred from the donor to L. vannamei[8, 22]. Most of the HGT candidates showed higher similarity with Arthropoda-related species and grouped within a single clade. Furthermore, several candidate HGTs showed a phylogenetic topology that did not support a HGT event. Thus, as for those 722 HGT candidates, 693 of them were eliminated by the phylogenetic analysis, leaving only 29 genes that displayed rational HGT phylogenetic topology (Figure 1). They were subjected to comparative phylogenetic analysis by constructing three kinds of phylogenetic trees. Ultimately, well-refined maximum likelihood (ML) trees were used to represent the HGT events.
Among the 29 candidate HGT genes above after phylogenetic selection, there were 12 genes showed significant similarity to the best-hit sequences (E-values ranged from 2.00E-82 to 5.00E-36, and identity values ranged from 95.83% to 100%). Considering that many candidate HGT genes might be contaminants derived from RNA extraction, library construction or sequencing, these sequences needed to be excluded from the 29 candidate HGT genes. Previously, we acquired 42× coverage of shotgun reads generated from whole genome sequencing of L. vannamei. A 1.9 Gb draft map of the contigs was assembled and used to identify genuine shrimp gene segments. Ultimately, only 14 candidate HGT genes were homologous to shrimp genomic contigs (E-values ranged from 0 to 9.00E-21, and identity values ranged from 88.22% to 100%). Thus, there are 15 genes were considered as non-shrimp genes and removed in this procedure. Some of HGT genes may be missed because of incompletely contigs assembly, but the 42× coverage of reads were full enough for identification of the existence of these gene segments. Generally, the sequenced reads tend to equally cover the assembly contigs, and eukaryotic contigs were expected to have different reads coverage to those contaminating contigs. Thus, with the help of SOAPaligner (http://soap.genomics.org.cn/soapaligner.html), we aligned all the reads to the 14 contigs which showed homologous with the 14 candidate HGT genes. The alignment results were compared with that of 1,000 randomly selected assembly contigs. It was found that the reads coverage of the 14 contigs (average coverage of 37.42) did not display any significant bias with that of 1,000 randomly selected contigs (average coverage of 38.25, Student’s t-test p < 0.05). Therefore, the 14 contigs seemed to possess eukaryotic characteristics rather than contaminates.
Fourteen predicted HGT genes in the L. vannamei genome
Top hit speciesb
B → L
acetyl-coenzyme A synthetase family protein
B → L
30S ribosomal protein S6
B → L
50S ribosomal protein L21
B → L
biopolymer transport protein
B → L
molybdopterin oxidoreductase, iron-sulfur binding subunit
B → L
B → L
B → L
repressor protein C2
B → L
B → L
B → L
short-chain dehydrogenase/reductase SDR
B → A
O-methyltransferase family protein
F → L
ankyrin repeat-containing protein
F → L
Bacteria and fungi were the two predominating donors of HGT genes
The phylogenetic trees can be used to indicate HGT events and their directions [22, 23]. For 11 of the 14 HGT genes, the phylogenetic trees showed a phylogenetic topology of L. vannamei nesting with bacteria but far from other eukaryotes, which indicated a bacteria-to-L. vannamei HGT event (Table 1, Additional file 2: Figures S1, S2, S3, S4, S5, S6, S7, S8, S9, S10 and S11). The most probable donors of bacteria-to-L. vannamei HGT genes were Proteobacteria (five species) and Bacterioidetes (four species), and most of them were ecologically related to shrimp. Desulfotomaculum kuznetsovii, Flavobacteriales bacterium, Robiginitalea biformata, Polaribacter irgensii, Gramella forsetii, and Vibrio harveyi are marine bacteria, while Escherichia coli and Bacteroides uniformis are gut bacteria. These results supported the view that HGT events generally occur between species inhabiting the same environment [1, 3]. The other two HGT genes were transferred from fungi to L. vannamei (ankp and deha, Table 1). Phylogenetic trees of ankp and deha showed that L. vannamei completely nested within the clade of fungi and far from bacteria and other eukaryotes, indicating a transfer of fungal origin (Additional file 2: Figures: S12, S13). The best-hit species of these two HGT genes belong to the Ascomycota, which is the largest phylum of fungi . One of the two fungi donors, Grosmannia clavigera, was reported to be a symbiont of the mountain pine beetle . Few fungi-to-higher eukaryote HGT events have been detected in previous studies, whereas, some reports of fungi-to-higher eukaryote HGT genes indicated that they greatly improved the host’s phenotypes and evolution [12, 25]. Subsequent functional analysis indicated that these two fungi originated HGT genes may function in protein-protein interactions and electron transfer, respectively.
Four HGT genes contain introns
Structure analysis of HGT genes was used to investigate whether there is structural evolvement of the HGT genes between the donor and recipient, which may provide evidence of the HGT events. Seven HGT genes were derived from single EST or transcriptome sequences: tnpA (GI: 41059456), stat (GI: 57504717), rpc2 (GI: 57504772), dhfr (GI: 171573934), cata (GI: 259510079), sdrp (GI: 349908938), deha (GI: 171560878). When comparing HGT gene segments to the shrimp genome contigs, we found that four HGT genes (acsf, sdrp, ankp and deha) contained introns in the shrimp genome. ankp and deha are the two HGT genes derived from fungi and their homologous genes in fungal genomes also contain introns, whereas acsf and sdrp were transferred from bacteria, which were free of introns. Surprisingly, it was found that the introns of genes from the donor fungi were different from those in the genome of shrimp, whereas the coding regions around the introns were highly conserved (E-values ranged from 8.00E-17 to 2.00E-13). Besides, by alignment of shrimp genome sequencing reads to this gene using SOAPaligner, we found that the intron regions were in highly coverage of nearly 88X, which is significantly higher than the sequencing depth of the whole genome (42X). Furthermore, these two HGT genes are evenly covered at 76X, while the random selected 100 genes is covered at 48X. Through using SOAPsnp v1.03 , three SNPs have been found on the contigs on which ankp located, and one of them has been detected locate on the intron of ankp. This indicated that the introns in two fungi-originated HGT genes may be highly repeated along with its surrounding sequences in shrimp genome. The shrimp acsf contains two introns (105 bp and 117 bp in length, respectively) and sdrp contains one intron (370 bp in length), but no introns were detected in the corresponding donor bacteria genome (D. kuznetsovii and B. phymatum). Therefore, it can be speculated that the introns might be integrated or changed in the HGT genes after horizontal transfer from the donor to L. vannamei.
Three large exogenous segments integrated into L. vannameigenome
HGT gene rpsF was predicted to encode 30S ribosomal protein S6, which is involved in protein synthesis (Table 1). When compared with the most probable donor genome of F. bacterium (NZ_ABI01000003), it was found that rpsF was almost in full-length like that on the donor genome (Figure 3b). Furthermore, it was also completely mapped with shrimp genome contigs without any integrated introns. The corresponding regions of rpsF on the shrimp and bacterial genomes were highly conserved (E value of 2.00E-59 and identity of 87.5%), which indicates that rpsF is an intron-less gene that may have transferred from bacteria to L. vannamei recently. Around rpsF, there is a large segment on the shrimp genome that is similar to the donor genome (E-values ranged from 2.00E-75 to 1.00E-11, identity values ranged from 78.01% to 92.31%). This large segment, 4,655 bp in length, contains six genes on a corresponding donor bacterial genome (Figure 3). Besides, it is surprising to found that non-coding regions on this segment were not conserved, and also a predicted gene hypo2 was not conserved as well. On donor bacterial genome, hypo2 is overlapped with both upstream hypo1 and downstream prsN. Homologous BLAST searching against the nt database detected a great many homologs (more than 100 matches) of this large segment, including hypo2. However, no evidence support hypo2 is actually a coding gene. If it is a gene prediction error, hypo2 may also a non-coding region. F. bacterium is just the best hit species of rpsF in GenBank database. It is known that there is a great deal of variation in genomes of bacteria. Therefore, the data of F. bacterium may be quite different from the true donor genome, which could lack hypo2. Thus, hypo2 may be missed in donor genome before the transfer of this large segment into shrimp genome. Whatever, the alignment results indicated that all these non-coding regions were absent from shrimp genome contigs. Similar results were also observed around mopB (Figure 3c).
The third horizontally transferred large DNA segment (4,118 bp) was around HGT gene mopB (Figure 3c). mopB is predicted to encode molybdopterin oxidoreductase and iron-sulfur binding subunit. Another molybdopterin oxidoreductase encoding gene, mopS, is located downstream of mopB and is also transferred to the shrimp genome. There is a region of low conservation (267 bp) upstream of mopB between the shrimp genome contig and the most probable donor genome. The alignment results indicated that most of the sequence (79.4%) from this region was absent in the bacterial genome. Eukaryotic gene prediction software packages including AUGEST , Glimmer-HMM  and FGENESH  were used to predict exons in this shrimp genome contig, and all the prediction results indicated that the low conserved region was part of a complete exon but not an intron that had inserted into the gene. This suggested that shrimp might have redirected this gene to integrate an additional coding sequence into the gene.
HGT genes under strong negative selection pressure
The d N /d S values of HGT genes
Most HGT genes are predicted to be associated with energy metabolism and defense mechanism
As for the six energy metabolism related HGT genes, it is of interest to find whether they are transferred from mitochondria in the distant past. It is well known that nuclear mitochondrial DNA (NUMTs) is commonly found to be transferred into nuclear genomes in various species . However, in this study, none of six energy metabolism related HGT genes have been identified to show any similarity to the whole mitochondrial genome of L. vannamei (NCBI accession number of NC_009626.1). Therefore, unlike NUMTs, these HGT genes are not transferred from mitochondria in recent past, but transferred from non-mating organisms of shrimp in the long evolutionary history.
In Figure 4, the other three HGT genes are predicted to function in protein synthesis and interaction. rpsF and rpsN are predicted to encode two ribosomal protein subunits that are used for protein synthesis. ankp encodes a protein containing ankyrin repeats, which are used for mediating protein-protein interactions in diverse families of proteins . Interestingly, the spread of ankyrin repeats has been suggested to have occurred by HGT, and their occurrence in yeast excludes exon shuffling . This supported the HGT events of ankp, which maintained the stability of its gene structure.
According to the annotated functions of four of the HGT genes, they are associated with defense mechanisms. There are two antibiotic resistance genes (stat and cata) that are predicted to encode streptomycin 3′-adenylyltransferase and chloramphenicol acetyltransferase, respectively (Table 1). Both transferases are responsible for the streptomycin and chloramphenicol resistance and prevent them from binding to ribosomes [50, 51]. Accidently, streptomycin and chloramphenicol are specifically bind to 30S and 50S ribosomal subunit, respectively, which are predicted encoded by two other HGT genes, rpsF and rpsN (Table 1). Thus stat and cata may be involved in protection of protein synthesis. As the only bacteria-to-Arthropoda HGT gene in L. vannamei (Figure 2), omtp was annotated to encode O-methyltransferase, which is ubiquitously dispersed in various organisms and plays an important role in animal growth, development and defense [52, 53]. rpc2 is annotated to encode repressor protein C2, which is a gene involved in the SOS response [54, 55]. Generally, the SOS response is induced by various antibiotics, such as chloramphenicol, trimethoprim and streptomycin. The SOS response can promote HGT events of antibiotic resistance genes among bacteria . Above all, the proteins encoded by these four HGT genes are predicted as defense-related.
Gene expression of the HGT genes in differential developmental stages of shrimp
Structure alteration of HGT genes indicated the mechanisms of HGT
Previous research on HGT events suggested that some of the HGT genes transferred from bacteria acquired eukaryotic features, such as signal peptides and spliceosomal-type introns [13, 37]. Similar occurrences were observed in the present work: five spliceosomal-type introns are integrated into four HGT genes in the shrimp genome. Two HGT genes originating from fungi (ankp and deha) seem to have differential introns to that of the most probable donor, while the other two HGT genes (acsf and sdrp) originated from bacteria and new introns were directly inserted into the HGT genes. As for ankp and deha, the introns on it may exchanged between donor and recipient genome, otherwise, it may originated from an un-sequenced fungus, of which the intron regions are also similar to that of shrimp genome. Whereas, the introns on ankp and deha are predicted to be highly repeated in shrimp genome, which indicated these introns maybe arose from the shrimp. Spliceosomal introns in eukaryotic genes have been identified as a possible barrier to HGT from fungi. However, it would still possible for fungi-originated genes transfer through mediation of spliced RNA intermediate . Thus, the introns on the two fungi-originated genes (ankp and deha) are most probably exchanged with shrimp introns rather than originated from un-sequenced fungi. Although introns are generally thought to be mildly deleterious elements for mature mRNA generation, there are still indications that some introns are essential for eukaryote genomes, e.g. alternative splicing, RNA editing and enhancing mRNA transcription . Thus, the introns integrated into these HGT genes may play essential roles in gene expression and regulation. In addition to introns, other non-coding DNA and even coding regions were changed in the HGT genes. The horizontally transferred large segment around rpsF was observed to exclude non-coding regions in the shrimp genome (Figure 3). By contrast, an inserted coding region was detected at the 5′ end of mopB in the shrimp genome. Although there is no evidence that this inserted coding sequence enhances the protein expression level or function, it may undergo an adaptive fitness in shrimp .
There are several mechanisms to explain HGT events in eukaryotes. The mechanism of bacteria-to-animal HGT most probably arises from endosymbiosis [1, 58]. The close and constant proximity of cells provides a platform for the transfer of genetic materials. Generally, the genetic materials that horizontally transferred are mostly complete or fragments of genes, but not large DNA fragments. However, horizontally transferred large DNA fragments were detected in previous studies and in the present study [59–62]. In the case of single HGT genes, spliceosomal-type introns were found, which indicated that these genes acquired the introns for their function after they were transferred to the host genome. In a previous study , several conserved introns in both recipient and donor taxa were found in HGT genes, which indicated that those HGT genes are the products of direct eukaryote-to-eukaryote transfer. By contrast, in this study, the introns in two of the fungi-to-L. vannamei HGT genes seem to be exchanged, which suggested the introns may have not have transferred into host genomes along with the exons. The single HGT genes, including intron-free HGT genes, were most probably acquired from mature RNA after gene transcription in the donor cell. It is also possible that these genes were acquired from raw DNA segments and shrimp could recognize the splice sites accurately and substitute with its own introns, which seems mechanistically unlikely. Operons have been reported playing important role in transfer of large DNA segments which include three or more genes . Through operon prediction by DOOR (Database of prOkaryotic OpeRons) , we found that this large horizontally transferred segment around rpsF was predicted as an operon. Furthermore, there is a gene priA, located upstream of this operon, encoded primosomal protein that are responsible for creating RNA primers during DNA replication . Primosomal proteins are essential for operon HGT events, and actually, primosomal operon have ever been detected in previous researches . Therefore, it is reasonable to think that the large segment around rpsF is completely transferred. As for the large horizontally transferred segment around mopB, operon prediction indicated that mopB and mopS are also involved in an operon, which implies that they are horizontally transferred together (Figure 3c). Although these large segments are transferred together, non-coding regions were removed from them (Figure 3, the large segment around tnpA was an exception because it was transferred as a single transposon), which suggested intergenic regions may not transferred into the shrimp genome. This supports the hypothesis that mature RNA was the main substrate for HGT.
Some HGT genes appeared to be functional in L. vannamei
Given that shrimp cells are frequently in proximity to prokaryotic cells, it is not surprising that HGT can be detected between them. The type of prokaryote-to-eukaryote HGT is generally of interest because it could potentially providing novel functions to eukaryotes, allow adaptation to novel niches, and affect their evolution . However, finding prokaryotic DNA in eukaryotic genome is not sufficient to imply that this DNA is functional in the host genome. In this study, several evidences suggest the functionality of these HGT genes for shrimp. Firstly, most of HGT genes had complete gene structure, and none of frameshift mutations could be found among them, which indicated they are not pseudogenes. Secondly, selection pressure analysis of these HGT genes showed that there was a strong negative selection for most of them (dN/dS values significantly lower than 1), indicating deleterious effects for most mutations at the protein levels , thus, these HGT genes in shrimp seemed to be effectively functional after the transfer. Lastly, these HGT sequences were derived from EST or transcriptome sequences, which imply they could transcribe in shrimp cells. Furthermore, most of the HGT genes were found to be transcribed at the early development stages of shrimp larvae, and they displayed significant DGE at five development stages. This indicated that these HGT genes were tightly associated with the development of shrimp. Therefore, these genes transferred from bacteria or fungi appeared to be functional in shrimp cells. According to the predicted functions of these HGT genes, six of them were annotated as energy metabolism related, and four of them were predicted to be associated with defense of shrimp. Among them, one of defense-related HGT gene, omtp, was reported to be involved in defense response against bacterial infection in Chinese shrimp, F. chinensis. In a previous report , there was strong evidence to show that invertebrates could obtain defensive complex polyketides from bacterial symbionts via O-methyltransferase (encoded by omtp) methylation of the marine compounds. Therefore, omtp was assumed to be an effective functional defense gene transferred from bacteria. Given the unlikely transfer and transcription of genes that in the donor are related to energy metabolism and defense, we speculate that these HGT genes may have the same role in the recipient. This is consistent with what is known about the demands of shrimp where molting is energy intensive and current aquaculture methods stressful [14, 16, 69]. However, the precise roles of these proteins need to be demonstrated through further studies.
Through sequence homology comparison, phylogenetic analysis and experimental verification, fourteen bacteria or fungi originated HGT genes were detected in L. vannamei. Spliceosomal-type introns were found to be inserted in four HGT genes, while non-coding regions of two large horizontally transferred segments were lacked on the shrimp genome. These structure alterations provide evidence that mature RNA may be the substrate for HGT events. Among 14 HGT genes, most of them were detected to be exposed to negative selection pressure and they appeared to be functional. Functions prediction annotated them to be associated with energy metabolism and defense of shrimp. Further studies should be taken to demonstrate the precise roles of these HGT genes.
Sources of genome and transcriptome dataset of L. vannamei
A total of 1.9 Gb shrimp genome contigs, which cover approximately 76% of the whole genome, were assembled from 42-fold coverage of whole genome shotgun reads of L. vannamei in our laboratory. The genome contigs were used for mapping of the candidate HGT genes on the shrimp genome. Information on the genome contigs to which candidate HGT genes mapped were submitted to NCBI (http://www.ncbi.nlm.nih.gov, KC701594 - KC701607).
The gene set of L. vannamei was predominantly composed of two online datasets from NCBI: 161,796 ESTs that were generated from multiple tissues [20, 70], and 74,111 transcriptome sequences that were generated from the whole body of L. vannamei larvae . The ESTs were assembled into 32,550 unigenes using CAP3 with default parameters . The software Minimus2 (http://sourceforge.net/apps/mediawiki/amos/index.php?title=Minimus2) was then used to merging the two sequence sets and remove duplicated, redundant sequences. Ultimately, 82,579 gene segments were generated and used for subsequent HGT genes detection.
Local protein database construction
There are 6,529,500 protein sequences collected from the complete proteomes of 4,080 species on the NCBI ftp site (ftp.ncbi.nlm.nih.gov), and these were used for HGT detection. The constructed local database included seven arthropods, 32 fungi, seven plants, 31 other eukaryotes, 1,607 bacteria and 2,387 viruses (Additional file 1: Table S1).
BLASTx-based HGT search and detection
BLASTx-based  homologous sequence searching was the first procedure for HGT detection, and there were three steps of similar sequences identification in the detection pipeline (Figure 1). First, each candidate L. vannamei gene segment was compared with the total protein sequences in the local database with cutoff thresholds of E value ≤ 1E-20, identity value ≥ 25% and overlap value ≥ 25 . The remaining 13,388 highest similarity sequences were extracted for further analysis. The second step was the similarity comparison between arthropods and other species. By comparing the BLAST results, candidate genes with higher BLASTx similarity scores to Arthropoda than other species were excluded because these genes were most probably the Arthropoda unique genes. In step three, using the same cutoff thresholds as the first step, the remaining sequences were used to search against the nr database. If there were less than 10 homologs in one phylogeny, the corresponding gene segments were not considered for subsequent phylogenetic analysis.
Homologous sequences of the remaining candidate HGT genes were extracted from the nr database, and the candidate HGT genes were translated from nucleotides to amino acid sequences. The combination of the candidate HGT genes sequences and their homologs from the nr database were used to construct phylogenetic trees, which were used to assess standard HGT or non-HGT events [22, 23]. Three types of phylogenetic tree were constructed based on different methods and were compared with each other to generate the best phylogenetic topology.
The first type of tree was generated by complement alignment of the sequences using ClustalX , and computing the pairwise distance-matrix of the aligned sequences with PHYLIP software package . A neighbor-joining tree was then constructed with the distance-matrix and 1000 bootstrap replicates were performed. The alignment method of the other two phylogenetic analyses was implemented on MUSCLE 3.6  and the conserved region of each alignment was trimmed using Gblocks , which allows less strict flanking positions and gap positions within the final blocks, but does not allow many contiguous nonconserved positions. Using Phyml , we performed an approximate likelihood-ratio test (aLRT, Minimum of SH-like and Chi2-based parametric) on the conserved aligned sequences , and constructed the phylogenetic tree with the substitution model of JTT. The maximum likelihood (ML) phylogeny was the last type of analysis, and the ML trees are shown in the figures. ML trees were constructed by Phyml using a WAG + gamma +Inv model, and 100% bootstraps were performed to gain the branch support values [8, 22]. The phylogenetic trees were used to judge HGT events and HGT directions, based on previous studies that considered that HGT genes tend to form a monophyletic branch with a set of far-related species, which are considered as the donors [22, 23]. The alignment datasets and phylogenetic tree-files of the HGT genes from analyses have been deposited in TreeBASE as study S14162 (http://www.treebase.org/treebase/). To test the support for contentious topology of the extracted HGT genes, Bayesian phylogenetic inference (BI) was performed on them with the help of the program Mrbayes 3.2.1 . In the BI analysis, two independent runs, each with four chains, were analyzed for millions of generations until the standard deviation of split frequencies converged towards zero. A burn-in of 25% samples is used for summarizing the parameter values and trees.
Verification of HGT genes by PCR
ESTs and transcriptome sequences may be contaminated by other organisms in the procedures of RNA extraction, library construction and sequencing; therefore, the candidate HGT genes were verified to confirm whether they were part of the genome of L. vannamei. Each candidate sequence that showed significant HGT events was compared against the draft genome contigs using BLASTn with a cutoff threshold of E value ≤ 1E-40, identity value ≥ 90% . To avoid the effects of genome mis-assembly, a DNA-based PCR was implemented to amplify the genomic segments containing HGT genes. DNA was isolated from the muscle tissue of the adult shrimps. The information of the genome contigs including HGT genes were extracted for primer design and the information of the primer pairs for each HGT gene are listed in Additional file 1: Table S2. Besides, PCR amplification was also performed on the edge of HGT fragments on genome contigs for the attempts of amplify both HGT region and eukaryotic portion of the genome. The edges of the HGT fragments were determined based on the BLAST (E value ≤ 1E-5) of the genome contigs against the most probable donor genome. The edges were the initial sites of two sides which showed homologous with the most probable donor genome. The primers were designed to amplify the products covering both HGT and eukaryotic portions as long as possible on both sides of the HGT region. The edge information and the primer pairs are listed in Additional file 1: Table S3. The PCR parameters were 94°C for 3 min; 35 cycles of 94°C for 30 s, annealing temperature (It is different for each product and are listed in Table S2-S3) for 30 s, and 72°C for 45 s; and a final 72°C for 7 min. The PCRs were conducted using ABI GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA). Besides, negative controls were set using Escherichia coli genomic DNA or distilled water as template for PCR amplification, and a positive control were performed using primers designed in previous study, which has been successfully amplified a specific shrimp genomic sequence (GI:124633758) . All the PCR products were sequenced to test their integrity and accuracy using ABI 3730xl DNA Analyzer (Applied Biosystems, Foster City, CA, USA). The sequences generated from sequencer were compared with the original genomic contigs.
Structure analysis of HGT genes
The structures of HGT segments and their surrounding sequences on both the shrimp and donor genome were analyzed. The tBLASTx program was used to compare the HGT segments and shrimp genome contigs against the complete donor genomes with an E value cutoff of 1.00E-10 in consideration of a longer match length. The up- and downstream genes of the HGT genes positions on the donor genome were identified and analyzed to determine whether they were co-transferred to shrimp genome. Using ClustalX, the conservation between shrimp genome contigs and donor genomes was displayed along the alignment sequences.
Calculation of dN/dSratios
We used KaKs_Calculator to calculate synonymous and nonsynonymous substitutions between orthologous pairs to identify whether there is natural selection subjected to these genes . The 46,676 transcriptome unigenes of F. chinensis and 7.8 Gb transcriptome reads of P. monodon were downloaded from NCBI (SRX110652, SRX110651 and SRX110649). The transcriptome reads of P. monodon were assembled into 182,648 unigenes through using the Trinity program . BLASTn-based homologous sequence searching was performed for predicted HGT genes of L. vannamei against the transcriptome unigenes of F. chinensis and P. monodon. The homologous sequences of each HGT gene were aligned using ClustalX and the open reading frames were determined, then, we calculated the dN/dS for each orthologous pair using YN algorithm with KaKs_Calculator.
DGE profiles of HGT genes
Set FPKM to be the expression of unigene A, and C to be number of fragments that uniquely aligned to unigene A, N to be total number of fragments that uniquely aligned to all unigenes, and L to be the base number in the CDS of unigene A. The FPKM method is able to eliminate the influence of different gene length and sequencing level on the calculation of gene expression. As the data came from five developmental stages for DGE analysis, with the help of the edgeR package , we performed 10 pairwise comparisons of the expression between any two developmental stages of shrimp. The DGE ratios, which were calculated using the ratio of the normalized FPKM values from any two samples, were used to test the statistical significance, as described recently [84, 85]. The p-values were analyzed using the edgeR package for significant differences, and q-values were calculated by applying a false discovery rate (FDR) adjustment for multiple testing [86, 87]. The criteria for DGE evaluation are DGE ratio ≥ 2, p value ≤ 0.001 and FDR ≤ 0.001.
Horizontal gene transfer
Expression sequence tags
Differential gene expression.
I would like to thank Dr. Feng Jiang for his thoughtful discussions on this work, and also thank Dr. Elayaraja Sivaramasamy for his help on editing the manuscript. This work was supported by a grant from National High Tech Research and Development Program (2012AA092205, 2012AA10A404) and National Program on Key Basic Research Project (973 Program, Grant No. 2012CB114403).
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