Evolution of male genitalia: environmental and genetic factors affect genital morphology in two Drosophila sibling species and their hybrids
© Soto et al; licensee BioMed Central Ltd. 2007
Received: 28 September 2006
Accepted: 15 May 2007
Published: 15 May 2007
The rapid evolution of genital morphology is a fascinating feature that accompanies many speciation events. However, the underlying patterns and explanatory processes remain to be settled. In this work we investigate the patterns of intraspecific variation and interspecific divergence in male genitalic morphology (size and shape) in the cactophilic sibling species Drosophila buzzatii and D. koepferae. Genital morphology in interspecific hybrids was examined and compared to the corresponding parental lines.
Despite of being siblings, D. buzzatii and D. koepferae showed contrasting patterns of genital morphological variation. Though genitalic size and shape variation have a significant genetic component in both species, shape varied across host cacti only in D. buzzatii. Such plastic expression of genital shape is the first evidence of the effect of rearing substrate on genitalic morphology in Drosophila. Hybrid genital morphology was not intermediate between parental species and the morphological resemblance to parental strains was cross-dependent.
Our results suggest the evolution of different developmental networks after interspecific divergence and the existence of a complex genetic architecture, involving genetic factors with major effects affecting genital morphology.
The evolutionary processes governing the divergence of animal genitalia are mostly unknown and constitute one of the most intriguing pieces of a mayor puzzle that is speciation [1–5]. In many arthropod groups male genitalia evolves at particularly high rates and this special feature constitutes the mechanistic basis of its use as a specific diagnostic trait [6, 7]. Notwithstanding the taxonomic importance of genitalic morphology, intraspecific studies addressing the causes and consequences of intraspecific morphological variation are scarce. Such studies, using methods successfully applied in evolutionary biology, offer the opportunity to gain new insights into the evolutionary processes and forces involved in genitalic evolution .
Three main hypotheses have been proposed to explain the evolution of genital morphology: the lock and key, the pleiotropy and the sexual selection hypotheses. The lock and key hypothesis  states that male genitalia evolve as a species-specific trait in order to properly fit in female genitalic organs. This theory predicts a canalized development of male genitalia and low levels of phenotypic and genotypic variation, since genitalic traits are expected to be under strong stabilizing selection [3, 9].
The pleiotropy hypothesis assumes that genital variation is largely neutral. Since genital and non-genital morphological traits are implicitly genetically correlated, changes of allele frequencies at loci pleiotropically affecting general morphology and genitalia may lead to rapid and arbitrary evolution of genitalic traits [2, 10, 11]. The sexual selection hypothesis states that morphological differences in male genitalia are related to variation in fertilization success and that morphological divergence is driven by sexual selection .
The study of the evolution of male genitalia may be even more complicated not only because it may be influenced by both natural and sexual selection, but also because it's phenotypic expression might be influenced by environmental factors  as occurs for other morphological traits. Thus, the joint study of intraspecific variation and interspecific divergence may provide a useful approach for the understanding of the underlying genetic architecture of genital traits and the evolutionary processes involved [13–15]. In this sense, it has been suggested that differences in genital morphology between closely related species would be largely polygenic . Such claim is based on the single study comparing the morphology of male posterior lobe in two closely related species of Drosophila and their hybrids . Therefore, it is clear that more studies are necessary to further support this affirmation and to determine whether simple genetic differences can account for the evolution of fast evolving and complex structures such as male genitalia.
The aedeagus, which is the intromittent organ of male genitalia , is considered the main diagnostic trait for species recognition in the Drosophila repleta group . To this group belong the South American D. buzzatii and D. koepferae [19, 20], which are morphologically undistinguishable except for conspicuous differences in male genitalia. These species are reproductively isolated by partial ecological isolation , sexual isolation and post mating barriers . Both species can breed and feed on the necrotic tissues of several cacti species [23, 24], however they exhibit a certain degree of niche separation; D. buzzatii is mainly adapted to breed on decaying tunas (genus Opuntia), while D. koepferae prefers the necrotic stems of columnar cacti of the genera Cereus and Trichocereus . Though sexual isolation between these species is strong, behavioral barriers can be forced in the laboratory, since D. buzzatii males can inseminate D. koepferae females and female hybrid offspring can be successfully backcrossed to D. buzzatii males . Furthermore, recent population genetic studies have provided indirect evidence of past or recent gene flow between these species [26, 27].
The knowledge of the ecology of these cactophilic Drosophila [21, 24, 28, 29], coupled with the possibility to produce interspecific hybrids in the laboratory and the potential for natural hybridization, makes this pair of species into an excellent model for speciation studies, particularly those addressing the genetic and ecological basis of morphological change associated to interspecific divergence.
In this work we investigate the sources of phenotypic variation, genetic and environmental, by examining genitalic size and shape in flies of several isofemale lines of both species and interspecific hybrids raised in two different species of host cacti.
A total of 606 males were analyzed in this study (252 D. buzzatii, 294 D. koepferae and 60 interspecific hybrids).
Parental species + hybrids
Parental species size and shape variation
Sources of variation of male genitalia in Drosophila buzzatii and D. koepferae.
Sources of variation
Species × Cactus
Line × Cactus
Line × Cactus
The results of intraspecific ANOVAs also revealed important differences (Table 2b). In D. buzzatii the Cactus by Line interaction was significant and accounted for a relatively high percentage (12.1%) of phenotypic variance in aedeagus size. However, only the Line factor was significant in D. koepferae, neither the Cactus effect nor the Cactus by Line interaction were significant.
In summary, according to our experimental design, based on the isofemale line technique , shows that aedeagus size is not only phenotypically plastic, but also that substantial heterogeneity exists among lines in their plastic response, suggesting that plasticity has a genetic basis in D. buzzatii. In D. koepferae, in contrast, our results show that variation in aedeagus size has a genetic component, devoid of any plastic response in relation to the breeding substrate. According to the results of the MANOVAs, variability among lines in aedeagus shape was significant in both species (Table 2a, b). The proportion of total shape variation explained by the interaction Cactus by Line also differed between species. Approximately 9% of total shape variance was explained by the Cactus by Line interaction in D. koepferae. This interaction was significant for PC3 which is related with variation in thickness in both dorsal and ventral median portions of the organ (Figure 1). Conversely, in D. buzzatii, the Cactus by Line interaction was significant for PC1, which describes changes in the process of the ventral margin (Figure 1) and accounts for an important proportion (30%) of the explained morphological variance.
Within organ allometry. Correlation coefficients between size and each one of the principal components (PC) scores accounting for shape variation in the aedeagus of D. koepferae, D. buzzatii and F1 hybrids.
Total allometric shape variance (%)
We also studied the relationship between variables describing size of male genitalia and wing length. These variables were not significantly correlated in D. koepferae (r = 0.13, p = 0.07), whereas in D. buzzatii, we detected a significant allometric relationship (r = 0.32, p < 0.001). Furthermore, aedeagus size and wing length varied isometrically in D. buzzatii as suggested by a coefficient of allometry not significantly different from 1 (slope value of linear adjusted function = 0.82; 95% confidence interval values [0.42 to 1.23]).
Aedeagus morphology in interspecific hybrids
Four interspecific crosses, out of 25 attempted, (crosses 4855, 4853, 8832 and 3512) yielded enough hybrid progeny as to perform the present study. These results are in agreement with previous studies reporting strong premating isolation between D. buzzatii and D. koepferae . In order to asses hybrid male fertility, and prior to dissection, hybrid males were aged for 1 week and placed for 5 days in vials with several mature virgin females of D. buzzatii or D. koepferae. In all cases hybrid males failed to produce offspring even though copulation attempts were observed in the vials. Hybrid progeny obtained in crosses 4853 and 8832 could only be tested in vials prepared with the medium prepared with fermenting Opuntia due to low numbers of hybrid larvae, while in the other crosses the yield of hybrid progeny was high enough to be reared in both cactus media.
Sources of variation of male genitalia interspecific crosses.
Sources of variation
Genotype × Cactus
Genotype × Cactus
Significant shape differences among genotypes were detected in all crosses (Table 4). Post hoc comparisons showed that all genotypes differed from each other in the shape of the genitalia at least in PC1 shape scores (p < 0.001 in all cases). However, neither the Cactus factor nor the Cactus by Genotype interaction were significant in the crosses in which hybrids were raised in the two cactus media (4855 and 3512).
Aedeagus morphology is a diagnostic trait that along with chromosomal inversions provides a guide for species identification in the genus Drosophila and particularly in the D. repleta species group [6, 19, 20, 32]. Several studies have, recently, turned the attention to the D. buzzatii cluster, a guild of cactophilic flies, in active cladogenesis, that inhabit the arid regions of Southern South America . However, recent molecular phylogenetic studies  cast doubts on the reliability of male genital (aedeagus) morphology to infer the evolutionary relationships in the D. buzzatii cluster . For instance, mitochondrial DNA sequence data place D. koepferae as the sister species of D. buzzatii, albeit the comparative analysis of aedeagus morphology indicates a close relationship with D. serido and allied species . These results indicate that rates of evolution of male genitalia may be heterogeneous among branches of the clusters' phylogenetic tree. In this context, our study, motivated by such inconsistency between molecular and morphological data, provides valuable information that could help to distinguish the relevant factors involved in morphological variation of male genitalia in D. buzzatii and D. koepferae, a pair of species in the heart of an evolutionary conflict.
The first issue raised by our study is that aedeagus size and shape vary substantially in both species, and, that a significant portion of variation is genetically determined. Moreover, the inclusion, in our experimental design, of semi-natural rearing media prepared with different cactus hosts permitted the characterization of morphological variation in terms of phenotypic plasticity in both species. In this sense, phenotypic plasticity in genital morphology was evident in D. buzzatii: flies emerged in alternative cactus hosts showed significant differences in aedeagus size and shape. Moreover, the analysis of the sources of variation also revealed a significant Line by Cactus interaction in D. buzzatii, i.e. isofemale lines did not respond in the same way to the two environments tested, suggesting that the plastic response of genital morphology has a genetic basis . Such a plastic expression of genital morphology constitutes the first evidence of a rearing substrate affecting genital morphology in Drosophila, and is in line with a recent study showing a plastic response of aedeagus morphology in relation to rearing temperature in D. mediopunctata . In contrast, comparisons between males of D. koepferae emerged in different cactus media did not reveal any sign of phenotypic plasticity, suggesting a more canalized (non plastic) development of male genitalia.
Another relevant point is that patterns of allometry within (aedeagus size and shape) and between organs (aedeagus and wing size) also differed between D. buzzatii and D. koepferae. In the latter, shape and size of male genitalia, as well as aedeagus size and wing length, appeared to be largely uncoupled as suggested by the low level of within and between organ allometries. These results suggest that differences among flies in wing length are not expected to be accompanied by changes in the size of the genitalia, indicating that the factors involved in development of wings and male genitalia are largely independent in D. koepferae. In contrast, aedeagus shape and size were significantly correlated in D. buzzatii, suggesting that factors affecting size (for instance the type of cactus host) may also indirectly affect the shape of the organ.
In D. buzzatii, body size related traits (such as wing length), known to be under natural selection [36–38], are affected by the nature of the breeding substrate [21, 39–41]. Furthermore, a positive correlation between body size and male reproductive success is well known in this species [38, 42–44]. If the phenotypic correlation observed between aedeagus size and wing length has a genetic basis, any directional selective pressure affecting body size (wing length) would indirectly affect the evolution of male genitalia.
Several features of the mating system, such as female remating frequency, premating time, copulation duration, interval between successive matings, and progeny numbers, have been shown to be genetically variable in D. buzzatii . However, the connection between these traits and male genital morphology has not been explored. Indeed, the implications of our present results in genital evolution and speciation would obviously depend on the kind of relationship between genital morphology and mating success, as it was reported to occur in other insect taxa such as Heteroptera  and Coleoptera .
To this point we have presented basic features of the patterns of variation of aedeagus morphology in D. buzzatii and D. koepferae, now, we would like to examine whether our data allow a critical evaluation of the plausibility of the three main hypotheses proposed to explain genital evolution. Though our results are not entirely conclusive in this respect, the extensive phenotypic and genetic variation in aedeagus morphology are strong evidence against the "lock and key" hypothesis, that predicts low levels of variation (both phenotypic and genetic) in genital structures. However, the correlation between aedeagus morphology and wing length, along with the condition dependence (phenotypic plasticity in relation to cactus hosts) are in agreement, at least in D. buzzatii, with predictions of the hypothesis of pleiotropy. Concerning the third hypothesis, sexual selection, we must await for the results of experiments testing the relationship, if any, between genital morphology and reproductive performance.
The final issue we would like to address is whether our study allows us to envisage the genetic architecture underlying differences in male genitalia between D. buzzatii and D. koepferae. In this sense, our results seem in conflict with the single available work comparing genital morphology in hybrids and parental species [17, 47]. Genital size and shape of hybrid males were not intermediate and the morphological resemblance of hybrids to either D. buzzatii or D. koepferae varied among crosses. In fact, hybrid's morphological distance to D. buzzatii and D. koepferae depended on the parental strains employed in the crosses. In none of the 4 crosses hybrid morphology was phenotypically intermediate (Figure 4). Hybrids from crosses 4853 and 8832 tended to be more similar to the D. koepferae parental strain, while in crosses 3512 and 4855 the morphology of the hybrid genitalia resembled closer that of D. buzzatii male parent. These results seem to be incompatible with the idea that interspecific differences in the morphology of male genitalia are caused by polygenes with small additive effects as claimed by Coyne and Orr for D. simulans and D. mauritiana  (however, it should be noted that the authors in  acknowledged some degree of dominance and epistasis). Actually, our results suggest a complex genetic architecture probably involving a certain degree of dominance and the involvement of genetic factors with large effect.
However, there are certain differences between our study and Liu et al's  that are worth mentioning. The first relates to the part of the genitalia examined in each case, the intromittent organ in D. buzzatii and D. koepferae and the posterior lobe (a particular element of male genitalia in the D. melanogaster group ) in D. simulans and D. mauritiana. These organs perform different functions during copulation  and therefore their evolution might be governed by different processes. The second is methodological and can be avoided by applying our methodology to Liu et al's dataset. To this end, we captured the outlines available in the digital version of , and tested for shape differences between F1 hybrids and parental species. This reanalysis confirmed that F1 hybrids have an intermediate morphology between D. simulans and D. mauritiana (the morphological distances between hybrids to both parental phenotypes were not significantly different: F1, 18 =, 008, p = 0, 93). Another non trivial point, that may complicate our interpretation is the difference in the time of divergence between the members of the two pairs of species, since development in interspecific hybrids is a result of a balance between the effects of the degree of heterozygosity and the degree of genomic coadaptation (expected to increase/decrease as a function of divergence, respectively) and the outcome of the past selection pressures on the species studied (see ). D. simulans and D. mauritiana are two recently derived species that shared their last common ancestor 0.6 – 0.9 million years ago , while D. koepferae and D. buzzatii are older species that diverged 5 million years ago . Finally, D. simulans and D. mauritiana are homosequential species, i.e. their basic polytene chromosome banding patterns are identical , whereas two inversions became fixed since divergence in D. buzzatii and D. koepferae. In addition, rich second chromosome inversion polymorphisms have evolved independently in the latter pair of species [20, 23]. Our knowledge of inversion polymorphisms is mostly restricted to D. buzzatii, in which polymorphic inversions are known to affect morphological and fitness related traits (see  and references therein). Although there is no direct evidence linking inversions and genital morphology, inversions may affect aedeagus morphology via its effect on general body size (recall the allometric relationship detected between aedeagus size and wing length in D. buzzatii). In this context, the idea that morphology (size and shape) of an organ potentially involved in species recognition (such as aedeagus morphology), might be associated to polymorphic inversions is consistent with recent theories linking chromosomal rearrangements and reproductive isolation [53, 54], and deserves further investigation.
Our comparative study of patterns of intraspecific variation in male genital size and shape and intra and inter-organ allometries in D. koepferae and D. buzzatii suggest different scenarios of genital evolution and probably the evolution of different developmental networks. Moreover, our study suggests that extrapolations across species are unwarranted, different evolutionary mechanisms might be involved in the evolution of genital morphology even in closely related species.
Fifteen isofemale lines (lines hereafter) of each species, derived from collections in the locality of Suyuque (San Luis province, Argentina), were employed in the experiments outlined below. In this area both species coexist in nearly equal proportions (45% of captured females were D. koepferae, 55% D. buzzatii). The advantages of the use of the isofemale line technique in quantitative evolutionary genetics have been thoroughly described in . Briefly, the isofemale line technique is a convenient methodology for the analysis of quantitative traits under laboratory conditions. All experiments described below were conducted 24 generations after the foundation of isofemale lines.
Thirty first-instar larvae from each line were seeded in vials containing 6 ml. of media prepared with artificially fermented cactus (see  for details). Two different cactus species, Opuntia sulphurea and Trichocereus candicans which are commonly used as breeding substrates by D. buzzatii and D. koepferae in the locality sampled, were employed for the preparation of the semi-natural media. For each line, 4 replicated vials were run in each cactus type.
Five lines of each species were randomly chosen to generate interspecific hybrids. All possible combinations were attempted (25 potential crosses). F1 hybrids were produced by crossing 25 virgin females of a D. koepferae line with 50 males of a D. buzzatii line. The reciprocal cross invariably failed to produce viable progeny. Crosses were identified according to the number of the female parental line (D. koepferae) preceding the number of the male parental (D. buzzatii) line (e.g. the cross between D. koepferae line 48 and D. buzzatii line 55 was designated as 4855). Batches of 30 first-instar hybrid larvae were transferred to culture vials containing one of the two 'semi-natural' media. Four replicated vials were run per every combination of cactus and cross when the number of hatched larvae allowed it. The hybrid status of the descendants was ascertained by the cytological analysis of the polytene chromosomes of progeny larvae grown in vials run in parallel.
All cultures were maintained at 25 +/- 1°C with a 12:12 light/dark photoperiod until the emergence of adults. Adult flies were simultaneously collected and sexed under light CO2 anesthesia.
The aedeagus and both wings were dissected from 2 to 5 males emerged in each replicate. Aedeagi were mounted on slides and photographed with a digital camera mounted on a microscope at 400 × magnification. Wings were also mounted and ventral views of wing images were captured with a digital camera attached to a binocular microscope (25 ×) connected to a computer. In each image, we scored total wing length (WL) using TpsDig .
The area of each aedeagus (in pixels) was calculated from the digital images and considered as an estimator of the size of the organ. We performed a normalization of the descriptors based on the first harmonic ellipse that corresponds to the first Fourier approximation to the contour information (reviewed in ). Thus, size, orientation and starting position of the contours were standardized in accordance with the size and alignment of the major axis of the first ellipse, leading to representations of the organs that are only based on internal properties of the outlines (i.e. shape) .
The variance-covariance matrix of the 120 (4 per harmonic) estimated EFDs coefficients was used as input in a principal components analysis. This procedure allowed us to summarize the information assessed in the coefficients and reduce the dimensionality of the variables  in a lower number of principal components (PC). Thus, the resulting scores of each PC of each specimen could be considered as reorganized uncorrelated morphological traits representing different aspects of total shape variation  that were used as shape descriptor variables in subsequent analyses. For the sake of simplicity and in order to avoid components accounting for possible biologically meaningless morphological variation only the first 5 components of both intraspecific and interspecific analyses were considered as shape variables in the ANOVAs.
We worked with two sets of PC scores. The first set of principal components were calculated from the matrix of coefficients derived form the analysis of the outlines of the genitalia of males of the parental lines employed in successful interspecific crosses and the hybrids. The second set was obtained separately for each species improving the assessment of intraspecific variation in aedeagus morphology by avoiding the noise resulting from conspicuous interspecific morphological differences in the estimation of the PCs. This set was used in the evaluation of intraspecific sources of shape variation. Preliminary analyses with this technique showed that it is repeatable and reliable in species discrimination .
Analysis of variance of aedeagus size and shape
Both interspecific and intraspecific size differences were investigated by means of ANOVAs, with Species (2 levels, fixed factor), Cactus (2 levels, fixed factor) and Line (nested in Species random factor) as main sources of variation. The variable was log-transformed to ensure homoscedasticity. According to our experimental design, in the ANOVAs for species, a significant cactus effect (C) may be interpreted as phenotypic plasticity, while significant differences among isofemale lines as due to genetic differences (since all lines were reared under the same conditions). Finally, a significant L × C interaction may be construed as an estimation of genotype by environment interaction (GEI) or more explicitly, that the response of isofemale lines is not independent of the rearing cactus.
Shape variation was assessed by means of MANOVAs using the scores of each PC as dependent variables and Cactus and Line as main sources of variation.
In the presence of allometry, a fraction of the changes in shape might be explained by changes in the size of the organ. We were interested to comparatively evaluate the amount of allometric change in genital morphology in these species. Thus, we calculated Pearson's product-moment correlation matrix among shape scores and aedeagus size in each species separately to explore the relationship between aedeagus shape and general size of the aedeagus (within organ allometry).
We also evaluated the allometric relationship between aedeagus size and wing length, a trait correlated with overall body size, which in the studied species are known to be affected by the rearing substrate . Size data were log-transformed prior to all analysis.
The principal components scores obtained by means of the general assessment of interspecific shape variation of parental lines and F1 hybrids were used in the examination of morphological patterns of variation in the offspring of interspecific crosses. Size was analyzed by means of an ANOVA and shape with a MANOVA. In both cases the principal factor was Genotype (both parents and the hybrids) and in those crosses in which hybrid larvae could be reared in both cactus hosts, Cactus was also considered as a fixed factor.
Statistica 6.0  was used for statistical analyses and in all cases the corresponding assumptions were properly tested.
We especially thank D Roccatagliata and M Mudry for help during the early stages of this research. E. Soto helped during the data acquisition and made insightful comments during the writing of the manuscript and A. Korovaichuk greatly influenced the final draft. We also wish to thank to two anonymous reviewers for comments that helped to improve this paper. We also thank other members of our research group for helpful discussion. IS and VC are recipients of postgraduate scholarships of CONICET and Universidad de Buenos Aires, respectively. JJF and EH are members of Carrera del Investigador Científico of CONICET. This work was supported by grants of Universidad de Buenos Aires, ANPCyT and CONICET awarded to EH.
- Coyne JA: Genetic basis of differences in genital morphology among three sibling species of Drosophila. Evolution. 1983, 37: 1101-1118. 10.2307/2408834.View ArticleGoogle Scholar
- Eberhard WG: Sexual selection and animal genitalia. 1985, Harvard Univ. Press: Cambridge, MAView ArticleGoogle Scholar
- Arnqvist G: The evolution of animal genitalia: distinguishing between hypotheses by single species studies. Biol J Linn Soc Lond. 1997, 60: 365-379. 10.1006/bijl.1996.0109.View ArticleGoogle Scholar
- Bond JE, Beamer DA, Hedin MC, Sierwald P: Gradual evolution of male genitalia in a sibling species complex of millipedes. Invertebr Syst. 2003, 17: 711-717. 10.1071/IS03026.View ArticleGoogle Scholar
- Hosken DJ, Stockley P: Sexual selection and genital evolution. Trends Ecol Evol. 2004, 19: 87-93. 10.1016/j.tree.2003.11.012.View ArticlePubMedGoogle Scholar
- Vilela CR: A revision of the Drosophila repleta species group (Diptera, Drosophilidae). Rev Bras Entomol. 1983, 1-114.Google Scholar
- Arnqvist G: Comparative evidence for the evolution of genitalia by sexual selection. Nature. 1998, 393: 784-786. 10.1038/31689.View ArticleGoogle Scholar
- Dufour L: Anatomie générale des Diptères. Annuaire de Science Naturelle. 1844, 1: 244-264.Google Scholar
- Pomiankowski A, Möller AP: A resolution of the lek paradox. Proc R Soc Lond, B, Biol Sci. 1995, 260: 21-29. 10.1098/rspb.1995.0054.View ArticleGoogle Scholar
- Mayr E: Animal species and evolution. 1963, Harvard University Pess: Cambridge, MA, USAView ArticleGoogle Scholar
- Eberhard WG: Animal genitalia and female choice. Am Sci. 1990, 78: 134-141.Google Scholar
- Andrade CAC, Hatadani LM, Klaczko LB: Phenotypic plasticity of the aedeagus of Drosophila mediopunctata: Effect of the temperature. J Therm Biol. 2005, 30: 518-523. 10.1016/j.jtherbio.2005.05.011.View ArticleGoogle Scholar
- Arnqvist G, Thornhill R: Evolution of animal genitalia: Patterns of phenotypic and genotypic variation and condition-dependence of genital and non-genital morphology in a water strider (Heteroptera: Gerridae). Genet Res. 1998, 71: 193-212. 10.1017/S0016672398003279.View ArticleGoogle Scholar
- Preziosi RF, Roff DA: Evidence of genetic isolation between sexually monomorphic and sexually dimorphic traits in the water strider Aquaruis remigis. Heredity. 1998, 81: 92-99. 10.1046/j.1365-2540.1998.00380.x.View ArticleGoogle Scholar
- House CM, Simmons LW: Genital morphology and fertilization success in the dung beetle Onthophagus taurus: an example of sexually selected male genitalia. Proc R Soc Lond, B, Biol Sci. 2003, 270: 447-455. 10.1098/rspb.2002.2266.View ArticleGoogle Scholar
- Coyne JA, Orr HA: Behavioral and nonecological isolation in Speciation. 2004, Sinauer Associates, Inc., Sunderland, Massachusetts USA, 231-232.Google Scholar
- Zeng Z, Liu J, Stam LF, Kao C, Mercer JM, Laurie CC: Genetic Architecture of a Morphological Shape Difference Between Two Drosophila Species. Genetics. 2001, 54: 299-310.Google Scholar
- Maggenti AR, Gardner S: Online Dictionary of Invertebrate Zoology: Complete Work. 2005, University of Nebraska – Lincoln, [http://digitalcommons.unl.edu/onlinedictinvertzoology/2]Google Scholar
- Wasserman M: Cytological evolution of the Drosophila repleta species group. Drosophila Inversion Polymorphism. Edited by: Krimbas CB, Powell JR. 1992, CRC Press, Boca Raton, FL, 455-552.Google Scholar
- Ruiz A, Wasserman M: Evolutionary cytogenetics of the Drosophila buzzatii species complex. Heredity. 1993, 70: 582-596.View ArticlePubMedGoogle Scholar
- Fanara JJ, Fontdevila A, Hasson E: Oviposition preference, viability, developmental time and body size in the cactophilic sibling species Drosophila koepferae and D. buzzatii in association to their natural hosts. Evol Ecol. 1999, 13: 173-190. 10.1023/A:1006636025623.View ArticleGoogle Scholar
- Naveira H, Fontdevila A: The evolutionary history of Drosophila buzzatii XII. The genetic basis of sterility in hybrids between D. buzzatii its sibling D. serido from Argentina. Genetics. 1986, 114: 841-857.PubMed CentralPubMedGoogle Scholar
- Fontdevila A, Pla C, Hasson E, Wasserman M, Sanchez A, Naveira H, Ruiz A: Drosophila koepferae new member of the Drosophila serido (Diptera, Drosophilidae) superspecies taxon. Ann Entomol Soc Am. 1988, 81: 380-385.View ArticleGoogle Scholar
- Hasson E, Fanara JJ, Rodríguez C, Vilardi JC, Reig OA, Fontdevila A: The evolutionary history of Drosophila buzzatii. XXIV: Second chromosome inversions have different average effect on thorax length. Heredity. 1992, 68: 557-563.View ArticlePubMedGoogle Scholar
- Marín I, Fontdevila A: Stable Drosophila buzzati-Drosophila koepferae hybrids. J Hered. 1998, 89: 336-339. 10.1093/jhered/89.4.336.View ArticlePubMedGoogle Scholar
- Gómez GA, Hasson E: Transpecific polymorphisms in an inversion linked esterase locus in Drosophila buzzatii. Mol Biol Evol. 2003, 20: 410-423. 10.1093/molbev/msg051.View ArticlePubMedGoogle Scholar
- Piccinali R, Aguadé M, Hasson E: Comparative Molecular Population Genetics of the Xdh Locus in the Cactophilic Sibling Species Drosophila buzzatii and D. koepferae. Mol Biol Evol. 2004, 21: 141-152. 10.1093/molbev/msh006.View ArticlePubMedGoogle Scholar
- Hasson E, Rodríguez C, Fanara JJ, Naveira H, Reig OA, Fontdevila A: Macrogeographic patterns in the inversion polymorphisms of Drosophila buzzatii in New World populations. J Evol Biol. 1995, 8: 369-384. 10.1046/j.1420-9101.1995.8030369.x.View ArticleGoogle Scholar
- Fanara JJ, Hasson E, Rodriguez C, Santos M, Fontdevila A: Are Opuntia hosts a selective factor for the inversion polymorphism?. Heredity. 1996, 77: 500-508.View ArticlePubMedGoogle Scholar
- David JR, Gibert P, Legout H, Pétavy G, Capy P, Moreteau B: Isofemale lines in Drosophila: an empirical approach to quantitative trait analysis in natural populations. Heredity. 2005, 94: 3-12. 10.1038/sj.hdy.6800562.View ArticlePubMedGoogle Scholar
- Marín I, Ruiz A, Pla C, Fontdevila A: Reproductive relationships among ten species of the Drosophila repleta group from South America from the West Indies. Evolution. 1993, 47: 1616-1624. 10.2307/2410173.View ArticleGoogle Scholar
- Ruiz A, Cansian AM, Kuhn GC, Alves MA, Sene FM: The Drosophila serido speciation puzzle: putting new pieces together. Genetica. 2000, 108 (3): 217-227. 10.1023/A:1004195007178.View ArticlePubMedGoogle Scholar
- Manfrin MH, Sene FM: Cactophilic Drosophila in South America: A Model for Evolutionary Studies. Genetica. 2006, 126: 57-75. 10.1007/s10709-005-1432-5.View ArticlePubMedGoogle Scholar
- Manfrin MH, de Brito ROA, Sene FM: Systematics and Evolution of the Drosophila buzzatii (Diptera: Drosophilidae) Cluster Using mtDNA. Ann Entomol Soc Am. 2001, 94: 333-346. 10.1603/0013-8746(2001)094[0333:SAEOTD]2.0.CO;2.View ArticleGoogle Scholar
- Conner JK, Hartl DL: A primer of ecological genetics. 2004, Sinauer Associates Sunderland, Mass USA, 138-Google Scholar
- Robertson FW: Studies in quantitative genetics. XI. Genetic and environmental correlation between body size and egg production in Drosophila melanogaster. J Genet. 1957, 55: 428-443.View ArticleGoogle Scholar
- Roff D: Dispersal in Dipterans: its costs and consequences. J Anim Ecol. 1977, 46: 443-456. 10.2307/3822.View ArticleGoogle Scholar
- Hasson E, Fanara JJ, Rodriguez C, Vilardi J, Reig OA, Fontdevila A: The evolutionary history of Drosophila buzzatii. XXVII. Thorax length is positively correlated with longevity in a natural population from Argentina. Genetica. 1993, 92: 61-65. 10.1007/BF00057508.View ArticlePubMedGoogle Scholar
- Fanara JJ, Mensch J, Folguera G, Hasson E: Developmental time and thorax length differences between the cactophilic species Drosophila buzzatii and D. koepferae reared in different natural hosts. Evol Ecol. 2004, 18: 203-214. 10.1023/B:EVEC.0000035043.32600.9d.View ArticleGoogle Scholar
- Fanara JJ, Folguera G, Fernández Iriarte P, Mensch J, Hasson E: Genotype by environment interactions in viability and developmental time in populations of cactophilic Drosophila. J Evol Biol. 2006, 19 (3): 900-908. 10.1111/j.1420-9101.2006.01084.x.View ArticlePubMedGoogle Scholar
- Carreira VP, Soto IM, Hasson E, Fanara JJ: Patterns of variation in wing morphology in the cactophilic Drosophila buzzatii and its sibling D. koepferae. J Evol Biol. 2006, 9 (4): 1275-1282. 10.1111/j.1420-9101.2005.01078.x.View ArticleGoogle Scholar
- Santos M, Ruiz A, Barbadilla A, Quezada-Díaz JE, Hasson E, Fontdevila A: The evolutionary history of Drosophila buzzatii. XlV. Larger flies mate more often in nature. Heredity. 1988, 61: 255-262.View ArticleGoogle Scholar
- Santos M, Ruiz A, Quezada-Diaz JE, Barbadilla A, Fontdevila A: The evolutionary history of Drosophila buzzatii. XX. Positive phenotypic covariance between field adult fitness components and body size. J Evol Biol. 1992, 5: 403-422. 10.1046/j.1420-9101.1992.5030403.x.View ArticleGoogle Scholar
- Norry FM, Vilardi JC, Fanara JJ, Hasson E: Courtship success and multivariate analysis of sexual selection on morphometric traits in Drosophila buzzatii (Diptera: Drosophilidae). Journal of Insect Behaviour. 1995, 8: 219-229. 10.1007/BF01988906.View ArticleGoogle Scholar
- Bundgaard J, Barker JSF: Remating, sperm transfer, and sperm displacement in the cactophilic species Drosophila buzzatii Patterson & Wheeler (Diptera: Drosophilidae). Biol J Linn Soc Lond. 2000, 71 (20): 145-164. 10.1006/bijl.1999.0433.View ArticleGoogle Scholar
- Bertin A, Fairbairn DJ: One tool, many uses: precopulatory sexual selection on genital morphology in Aquarius remigis. J Evol Biol. 2005, 18 (4): 949-961. 10.1111/j.1420-9101.2005.00913.x.View ArticlePubMedGoogle Scholar
- Liu J, Mercer JM, Stam LF, Gibson GC, Zeng ZB, Laurie CC: Genetic Analysis of a Morphological Shape Difference in the Male Genitalia of Drosophila simulans and D. mauritiana. Genetics. 1996, 142: 1129-1145.PubMed CentralPubMedGoogle Scholar
- Jagadeeshan S, Singh RS: A time-sequence functional analysis of mating behaviour and genital coupling in Drosophila: role of cryptic female choice and male sex-drive in the evolution of male genitalia. J Evol Biol. 2006, 19 (4): 1058-1070. 10.1111/j.1420-9101.2006.01099.x.View ArticlePubMedGoogle Scholar
- Alibert P, Auffray JC: Genomic coadaptation, outbreeding depression, and developmental stability. Developmental Instability: Causes and Consequences. Edited by: Polak M. 2003, Oxford University Press, Oxford, 116-134.Google Scholar
- Hey J, Kliman RM: Population genetics and phylogenetics of DNA sequence variation at multiple loci within the Drosophila melanogaster species complex. Mol Biol Evol. 1993, 10: 804-822.PubMedGoogle Scholar
- Lemeunier F, Ashburner M: Relationships within the melanogaster species subgroup of the genus Drosophila II. Phylogenetic relationships between six species based upon polytene chromosome banding sequences. Proc R Soc Lond, B, Biol Sci. 1976, 193: 275-294.View ArticlePubMedGoogle Scholar
- Fernández Iriarte P, Hasson E: The role of the use of different host plants in the maintenance of the inversion polymorphism in the cactophilic Drosophila buzzatii. Evolution. 2000, 54: 1295-1302.View ArticlePubMedGoogle Scholar
- Noor MAF, Grams KL, Bertucci LA, Reiland J: Chromosomal inversions and the reproductive isolation of species. Proc Natl Acad Sci USA. 2001, 98: 12084-12088. 10.1073/pnas.221274498.PubMed CentralView ArticlePubMedGoogle Scholar
- Rieseberg LH: Chromosomal rearrangements and speciation. Trends Ecol Evol. 2001, 16: 351-358. 10.1016/S0169-5347(01)02187-5.View ArticlePubMedGoogle Scholar
- Rohlf FJ: TpsDig, v 1.31 Free software. 2001, Ecology & Evolution, SUNY, Stony Brook, [http://life.bio.sunysb.edu/morph/index.html]Google Scholar
- Kuhl FP, Giardina CR: Elliptic Fourier features of a closed contour. Computer Graphics and Image Processing. 1982, 18: 236-258. 10.1016/0146-664X(82)90034-X.View ArticleGoogle Scholar
- Iwata H, Ukai Y: SHAPE: A Computer Program Package for Quantitative Evaluation of Biological Shapes Based on Elliptic Fourier Descriptors. J Hered. 2002, 93: 384-385. 10.1093/jhered/93.5.384.View ArticlePubMedGoogle Scholar
- Ferson S, Rohlf FJ, Koehn RK: Measuring shape variation of two-dimensional outlines. Syst Zool. 1985, 34: 59-68. 10.2307/2413345.View ArticleGoogle Scholar
- Rohlf FJ: Fitting curves to outlines. Proceedings of the Michigan morphometrics workshop. Edited by: Rohlf FJ, Bookstein FL. 1990, 167-177.Google Scholar
- Lestrel PE: Fourier Descriptors and Their Applications in Biology. 1997, Cambridge University Press. UK, 480-View ArticleGoogle Scholar
- Rohlf FJ, Archie JW: A comparison of Fourier methods for the description of wing shape in mosquitoes (Diptera: Culicidae). Systematic Biology. 1984, 33: 302-317.Google Scholar
- Soto I: Use of elliptic Fourier descriptors for quantification of male genitalia morphology. Drosoph Inf Serv. 2005, 88: 42-45.Google Scholar
- StatSoft, Inc. STATISTICA (data analysis software system), version 6. 2001, [http://www.statsoft.com]
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.