The medaka novel immune-type receptor (NITR) gene clusters reveal an extraordinary degree of divergence in variable domains
© Desai et al; licensee BioMed Central Ltd. 2008
Received: 02 April 2008
Accepted: 19 June 2008
Published: 19 June 2008
Novel immune-type receptor (NITR) genes are members of diversified multigene families that are found in bony fish and encode type I transmembrane proteins containing one or two extracellular immunoglobulin (Ig) domains. The majority of NITRs can be classified as inhibitory receptors that possess cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs). A much smaller number of NITRs can be classified as activating receptors by the lack of cytoplasmic ITIMs and presence of a positively charged residue within their transmembrane domain, which permits partnering with an activating adaptor protein.
Forty-four NITR genes in medaka (Oryzias latipes) are located in three gene clusters on chromosomes 10, 18 and 21 and can be organized into 24 families including inhibitory and activating forms. The particularly large dataset acquired in medaka makes direct comparison possible to another complete dataset acquired in zebrafish in which NITRs are localized in two clusters on different chromosomes. The two largest medaka NITR gene clusters share conserved synteny with the two zebrafish NITR gene clusters. Shared synteny between NITRs and CD8A/CD8B is limited but consistent with a potential common ancestry.
Comprehensive phylogenetic analyses between the complete datasets of NITRs from medaka and zebrafish indicate multiple species-specific expansions of different families of NITRs. The patterns of sequence variation among gene family members are consistent with recent birth-and-death events. Similar effects have been observed with mammalian immunoglobulin (Ig), T cell antigen receptor (TCR) and killer cell immunoglobulin-like receptor (KIR) genes. NITRs likely diverged along an independent pathway from that of the somatically rearranging antigen binding receptors but have undergone parallel evolution of V family diversity.
The two major forms of novel immune-type receptors (NITRs) possess either one N-terminal ectodomain of the Ig variable (V) type, which may contain a joining (J) or J-like sequence or an N-terminal V domain plus a second C-terminal Ig domain of the intermediate (I) type. Most NITRs are predicted to encode type I transmembrane cell surface proteins that possess extracellular immunoglobulin (Ig) domains and can be classified as inhibitory or activating. A smaller number of NITR genes lack a transmembrane domain and are predicted to be secreted proteins [1, 2]. NITRs are expressed in several different hematopoietic lineages [3, 4] and their overall structure and cytoplasmic signaling resemble the mammalian natural killer cell receptors (e.g. KIRs) [1, 5, 6]. NITRs have been reported to bind allogeneic cell surface targets, although their precise ligand specificity is not clear [Cannon et al., unpublished observations and ]. Other observations, including upregulation of transcription in response to pathogens, support a role for NITRs in immunity .
NITRs were identified initially in the compact genome of the Southern pufferfish (Spheroides nephelus); more than 26 NITR genes were shown to be encoded in a single gene cluster [9, 10]. Subsequent analysis of the zebrafish (Danio rerio) genome revealed one large NITR gene cluster encoding 36 NITRs on chromosome 7 and a second smaller NITR gene cluster encoding 3 NITRs on chromosome 14 [2, 11]. NITR cDNAs also have been characterized from channel catfish (Ictalurus punctatus), rainbow trout (Oncorhynchus mykiss) and Japanese flounder (Paralichthys olivaceus) [3, 4, 12, 13] and it is assumed that they are present in all bony fish. The majority of NITRs possess cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs); inhibitory functions have been confirmed in vitro . A smaller number of NITRs possess a positively charged residue within their transmembrane domain and have been shown to interact with Dap12 through which they mediate an activating function .
We herein report the identification of 44 medaka (Oryzias latipes) NITR genes, which can be classified into 24 families including inhibitory and activating forms; this represents nearly twice the number of NITR families that have been identified in any other species. The resolution of this dataset of NITRs along with the previous genomic resolution of NITRs in zebrafish permits a comprehensive phylogenetic analysis as well as the examination of shared synteny. The NITRs emerge as independent families of immune-type receptors in which the evolution of the V regions exhibit considerable similarity to that seen in multigene families encoding Ig, TCRs and KIRs.
Medaka encode 44 NITRs on three chromosomes
Two major classes of NITR gene organization
All of the NITR genes currently described from the Southern pufferfish and 11 NITR genes from a second pufferfish species, Tetraodon nigroviridis (Additional file 3), encode two Ig domains (V and I domains) in a single exon . In contrast, all of the two-Ig domain NITR genes described from zebrafish encode the V and I domains in two exons . Both types of NITR gene organization are present in medaka (Figure 2). The medaka NITR genes encoding two Ig domains in a single exon are all located on chromosomes 18 and 21; the medaka NITR genes encoding two Ig domains in two exons are located on chromosome 10 with the exception of the NITR9 genes, which are on chromosome 18.
Twenty-one full-length NITR cDNAs were cloned from medaka kidney and spleen by 3' RACE using nested primers that include the predicted translational start codon (Additional Files 4 and 5) in order to verify the exon-intron boundaries for the NITRs. The predicted exon-intron boundaries most often were verified; however, in several cases, predicted mRNA splice sites were inexact, reflected slight differences in cDNA sequence. It is likely that the genome from inbred Hd-rR medaka and cDNAs from the out-bred orange-red medaka line possess different alleles for certain NITRs. For example, the identification of a NITR6 cDNA from the orange-red line confirms that the NITR6 genes possess a cytoplasmic tail; however, the cDNA cytoplasmic sequence is too divergent to confidently predict these exons from the Hd-rR genome. In addition the orange-red NITR12 cDNA encodes additional nucleotide sequences that are not present in the Hd-rR genome resulting in a stop codon preventing translation of an ITIM-like sequence (itim) in the cDNA (Additional File 6).
Structural features of medaka NITRs
NITR gene clusters in medaka and zebrafish share conserved synteny
Evidence for conserved synteny between NITR gene clusters
CHRNB3 (2 of 2)
FGFRL1 (2 of 2)
A bony fish SPON2/FGFRL1/NITR locus shares conserved synteny with the SPON2/FGFRL1/CD8 locus in Xenopusand chicken
NITRs have not been identified outside of bony fish and their evolutionary origins remain unresolved. The genomes of human, chicken, Xenopus tropicalis and a third fish species stickleback (Gasterosteus aculeatus) were queried in an effort to identify genomic regions, which share conserved synteny with the NITR gene clusters. In medaka, zebrafish and stickleback, one NITR gene cluster is linked to SPON2 and FGFRL1 orthologs. In chicken and Xenopus the SPON2 and FGFRL1 genes are physically linked to the CD8A/CD8B locus (Figure 5). SPON2/FGFRL1 and CD8A/CD8B genes are on different chromosomes in human. In medaka, zebrafish and stickleback the CD8A/CD8B locus does not display conserved synteny to human (Additional File 10). The observation that SPON2 and FGFRL are linked to the CD8 locus in chicken and Xenopus and that SPON2 and FGFRL orthologs are linked to a NITR gene cluster in medaka, zebrafish and stickleback is consistent with a common ancestry for these immune-type molecules.
Medaka NITR24 is tightly linked to a CTLA4ortholog
The NITR24 locus on medaka chromosome 21 is separated from the V-domain receptor CTLA4 by ~0.1 Mb. A small number of predicted olfactory receptor genes lie between CTLA4 and NITR24 (Additional File 11). Although mammalian CTLA4 is tightly linked to CD28, a structurally similar receptor, these genes do not appear to be closely linked in bony fish . Neither NITR gene cluster in zebrafish is linked to CTLA4 or CD28.
Species-specific expansions of NITR families
In general, most NITRs from a single species lack clear orthologs in other species. The absence of such comparison sets skews multi-species phylogenetic comparisons as not all NITR members from each species (and family) have been identified. Three major inferences can be made from the data described here (Additional File 12) and other observations predicted previously: 1) the activating receptors, medaka NITR9, zebrafish Nitr9, and catfish IpNITR2, group together suggesting a common ancestry; 2) the majority of the NITRs encoding both V and I domains within a single exon (medaka, Southern pufferfish and Tetraodon) group together, suggesting that they are likely derived from a common ancestral gene; and 3) the majority of the NITRs, which lack an I domain (from medaka, zebrafish, catfish, and Tetraodon), group together, suggesting that they too may be derived from a common ancestral gene.
Interpreting the evolution of NITRs within teleost species
Forty-four different NITR genes, the majority of which are inhibitory, are present in the medaka genome. Perhaps the most striking observation relates to the high degree of variation between these V domains within medaka and their diversification from NITRs in other fish species. This finding is particularly striking given the exceptionally large number of V families in medaka, i.e., the greater the number of V families present, the higher the expectations of identifying orthologous sequences. The medaka/zebrafish chromosomes 18/7 and 10/14, which encode the larger NITR gene clusters, exhibit a high degree of conserved synteny . Medaka chromosomes 10 and 18, and zebrafish chromosomes 7 and 14 likely derive from a hypothetical ancestral chromosome termed "f" which was duplicated prior to the divergence of medaka and zebrafish . Although the nucleotide identity is low between medaka and zebrafish NITR genes (confounding the confident assignment of genetic orthologs), conserved synteny between the two primary medaka and zebrafish NITR gene clusters is impressive.
Comparisons of predicted amino acid sequence structures within and between different species indicate that NITRs are undergoing family-specific expansions and individual genes are undergoing birth and death events. As the NITR gene products are highly diverse within a single species (Figures 3 and 4) and approximately 10% of the NITRs described in medaka can be classified as pseudogenes (Figure 1), it is less likely that concerted evolution played a primary role in establishing the current NITR sequences . Birth and death events of NITR genes likely have been occurring continuously after the divergence of medaka and zebrafish ~323 MYA . Similar observations have been made concerning the V gene segments of the Ig and TCR genes  and the KIR genes within primate species [reviewed by ]. The KIR gene family is of particular value in comparative interpretations as they also represent type I, Ig domain-containing membrane proteins with potential for inhibitory and activating signalling. KIRs are highly polymorphic and polygenic within single species and drastically diverged in gene sequence and number between different primates . The nature of diversity in KIRs is markedly similar to that observed in the NITRs of medaka and zebrafish. The studies reported here suggest that particularly extensive expansion and diversification of the NITRs have occurred in bony fish. Deducing the status of the NITR gene family from the genomes of more basal fish species (such as gar or sturgeon) will be highly informative regarding the origins of diversity within the NITR family.
Possible links between NITRs and mammalian receptors
All evidence to date strongly suggests that NITRs are restricted to bony fish. Although NITRs possess authentic V (and frequently V-J) domains reminiscent of TCR, their overall structure, genomic organization and the presence of both activating and inhibitory forms, has led to the hypothesis that they may be functional equivalents to mammalian NK receptors, resembling in some way KIRs. We previously reported that the zebrafish NITR gene cluster on chromosome 7 shares a limited syntenic relationship with the mammalian leukocyte receptor cluster (LRC) that includes the KIRs . The new data presented here introduces two alternative models in which the NITRs share a common ancestry with CD8A/CD8B or with CD28/CTLA4. An expansion of CD8A-like genes was recently described within the chicken CD8A/CD8B locus; the reported structural similarity between the V-like domains of the CD8A-like receptors and NITRs supports the former model . Although intriguing, the latter model is not as well supported. Specifically, in mammals CD28 and CTLA4 effect opposing roles in T-cell stimulation and are encoded by adjacent genes. However, the CD28 and CTLA4 genes are not physically linked in teleosts. Linkage of an NITR gene to CTLA4 has not been identified outside of medaka; in zebrafish the NITR gene clusters are on chromosomes 7 and 14 [2, 11]. CTLA4 and CD28 genes in zebrafish are located on chromosomes 9 and 1, respectively .
KIRs, CD8, CD28 and CTLA4 are expressed by cytotoxic lymphocytes and NITRs are similarly expressed in lymphoid populations including NK-like cytotoxic cells [unpublished observations and [3, 4]]. In general, inhibitory natural killer cells receptors (NKRs) repress cytotoxicity by recognition of MHC or other markers of "self" on candidate target cells whereas activating NKRs induce cytotoxicity by detecting a loss of "self" or a gain of "non-self" (e.g. viral or stress-related peptides). In at least one case, a NITR specifically engages a non-self determinant (Cannon et al., unpublished observations), suggesting the possibility that they may be involved in cytotoxic interactions. Ongoing efforts to identify NITR ligands and define cell populations that express NITRs in vivo will prove crucial in deciphering their role in immunity. In particular, such studies will determine whether their function is analogous to mammalian processes (NK recognition) or represents a unique adaptation within bony fish.
The medaka genome encodes 44 NITR genes in 3 gene clusters. NITR genes in this species are divergent in sequence but structurally similar to those characterized in other fish species and include both inhibitory and activating forms. Comparative genomics reveals three possible scenarios in which the NITRs share a common genetic ancestry with CD8, CD28/CTLA4 or KIRs. A comparison of the complete sets of NITRs from medaka and zebrafish indicate that: 1) the two largest NITR gene clusters in medaka and zebrafish are derived from a whole chromosome duplication event, 2) as a gene family, the NITRs have experienced recent, species-specific "birth and death" events and 3) NITR family expansions have led to high levels of sequence variation between fish species.
Using zebrafish nitr1c and nitr3a as queries, putative medaka NITR genes were identified by searching version 1.0 of the medaka genome . Manual annotation of these medaka genes was performed using FSPLICE to predict splice sites (GT/AG) and FGENESH+ to predict structures based on homology with zebrafish and other medaka NITR genes: "fish" specific parameters were applied to both programs which are included in the MolQuest software suite . SEQtools was used to visualize the predicted transcripts and their three-frame translations . In addition, V and I domain predictions were validated by searching the NCBI non-redundant database with BLAST , while transmembrane predictions were validated using the TMHMM 2.0 server . Lastly, groups of medaka NITRs were defined through BLASTCLUST (single-linkage) based on sharing 70% identity within the peptide sequence of the V-domain. Orthologous genes and their genomic positions were identified in medaka, zebrafish, stickleback, Xenopus tropicalis, chicken, mouse and human genomes using ContigView software within the Ensembl genome browser .
Published NITR V or I domains were aligned by ClustalW  and neighbor-joining trees  were constructed from pairwise Poisson correction distances with 2000 bootstrap replications by MEGA2.1 software . NITR sequences described from medaka, zebrafish, Southern pufferfish, channel catfish, rainbow trout, Japanese flounder and Tetraodon (Additional file 3) were included in alignments [2–4, 10–14].
Rapid Amplification of cDNA ends
Tissues from young adult (6–9 month), out-bred, orange-red medaka were dissected directly into RNA-Bee and total RNA purified as recommended by the manufacturer (Tel-Test, Friendswood, TX). Two μg of RNA (1 μg from kidney and 1 μg from spleen) were reverse transcribed using the oligo dT primer and Superscript™ III Reverse Transcriptase supplied with the GeneRacer™ kit (Invitrogen, Carlsbad, CA). Nested, forward primers were designed against the exons encoding predicted leader sequences of medaka NITRs and 3' RACE was completed as recommended in the GeneRacer kit. Primer sequences are listed in Additional File 4. PCR products were cloned into pCRII-TOPO (Invitrogen) or pGEM-T Easy (Promega, Madison, WI) and sequenced.
Sequence data described in this article have been deposited with the GenBank database under accession numbers: NITR1, transcript variant 1 [GenBank:EU419354]; NITR1, transcript variant 2 [GenBank:EU419355]; NITR1, transcript variant 3 [GenBank:EU419356]; NITR2, transcript variant 1 [GenBank:EU419357]; NITR3, transcript variant 1 [GenBank:EU419358]; NITR4, transcript variant 1 [GenBank:EU419359]; NITR5, transcript variant 1 [GenBank:EU419360]; NITR5, transcript variant 2 [GenBank:EU419361]; NITR6, transcript variant 1 [GenBank:EU419362]; NITR8, transcript variant 1 [GenBank:EU419363]; NITR9, transcript variant 1 [GenBank:EU419364]; NITR11, transcript variant 1 [GenBank:EU419365]; NITR12, transcript variant 1 [GenBank:EU419366]; NITR14, transcript variant 1 [GenBank:EU419367]; NITR17, transcript variant 1 [GenBank:EU419368]; NITR18, transcript variant 1 [GenBank:EU419369]; NITR20, transcript variant 1 [GenBank:EU419370]; NITR22, transcript variant 1 [GenBank:EU419371]; NITR24, transcript variant 1 [GenBank:EU419372]; NITR24, transcript variant 2 [GenBank:EU419373]; and NITR24, transcript variant 3 [GenBank:EU419374].
We are indebted to Dave Hinton, Seth Kullman and Sheran Law for medaka tissues. We thank Chris Amemiya for very helpful discussions and advice on phylogenetic relationships, Jacques Robert and Alexander Taranin for sharing the chromosomal location of Xenopus tropicalis CD8B and Barbara Pryor for editorial assistance. This work was supported by grant R01 AI057559 from NIH.
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