From sea to land and beyond – New insights into the evolution of euthyneuran Gastropoda (Mollusca)
© Klussmann-Kolb et al; licensee BioMed Central Ltd. 2008
Received: 02 August 2007
Accepted: 25 February 2008
Published: 25 February 2008
The Euthyneura are considered to be the most successful and diverse group of Gastropoda. Phylogenetically, they are riven with controversy. Previous morphology-based phylogenetic studies have been greatly hampered by rampant parallelism in morphological characters or by incomplete taxon sampling. Based on sequences of nuclear 18S rRNA and 28S rRNA as well as mitochondrial 16S rRNA and COI DNA from 56 taxa, we reconstructed the phylogeny of Euthyneura utilising Maximum Likelihood and Bayesian inference methods. The evolution of colonization of freshwater and terrestrial habitats by pulmonate Euthyneura, considered crucial in the evolution of this group of Gastropoda, is reconstructed with Bayesian approaches.
We found several well supported clades within Euthyneura, however, we could not confirm the traditional classification, since Pulmonata are paraphyletic and Opistobranchia are either polyphyletic or paraphyletic with several clades clearly distinguishable. Sacoglossa appear separately from the rest of the Opisthobranchia as sister taxon to basal Pulmonata. Within Pulmonata, Basommatophora are paraphyletic and Hygrophila and Eupulmonata form monophyletic clades. Pyramidelloidea are placed within Euthyneura rendering the Euthyneura paraphyletic.
Based on the current phylogeny, it can be proposed for the first time that invasion of freshwater by Pulmonata is a unique evolutionary event and has taken place directly from the marine environment via an aquatic pathway. The origin of colonisation of terrestrial habitats is seeded in marginal zones and has probably occurred via estuaries or semi-terrestrial habitats such as mangroves.
Within the phylum Mollusca, Gastropoda represent the largest and most diverse group in terms of species numbers, niche selection and life history strategies. They have been traditionally classified into three main subclasses: Prosobranchia, Opisthobranchia and Pulmonata [1–3]. Within Gastropoda, Opisthobranchia and Pulmonata have been united as Euthyneura and have since Spengel  been contrasted to the Streptoneura (= Prosobranchia). The latter, however, are commonly accepted as being paraphyletic .
Although a plethora of morphological and anatomical data on Gastropoda have accumulated over the last centuries, it was not until the 1980s that the development of cladistic methodology allowed for analysing these data in a phylogenetic framework e. g. [5–9]. Nevertheless, euthyneuran gastropods have often been neglected in these studies.
Several new studies based on morphological data have indicated that Euthyneura is a taxon clearly distinct from the remaining Gastropoda, belonging to a larger monophyletic group, the Heterobranchia. The latter also include the paraphyletic Heterostropha . Euthyneura are characterised by several autapomorphies . However, difficulties with respect to establishing a natural system of Euthyneura are well known  and can be attributed to the large number of homoplasies and convergent evolution of character traits.
Recent phylogenetic studies have focussed on several subgroups of Euthyneura, producing partly conflicting results.
Pulmonata have been both analysed morphologically [6, 13] and on the basis of molecular data [14, 15]. They have mostly been recovered monophyletic [6, 8, 11, 13, 16]. However, phylogenetic relationships of subgroups within Pulmonata have not been conclusively resolved yet. [11, 17].
Opisthobranchia are often rendered paraphyletic in phylogenetic analyses regardless of morphological or molecular systematic approaches [11, 18–22]. Moreover, positions of enigmatic taxa within the phylogenetic system of Opisthobranchia, such as the Sacoglossa, Acochlidiacea, Umbraculoidea or Acteonoidea also remain unresolved so far [21, 22].
To date, no sound phylogenetic hypothesis for the Euthyneura exists. Recent morphological analyses by Dayrat and Tillier  yielded very poor resolution within Euthyneura and demonstrated the need to explore new datasets in order to critically analyse the phylogeny of this controversial group of gastropods. Upcoming molecular systematic studies have mostly utilised single genetic markers comprising partial sequences only  or have not included all major lineages particularly respective to Pulmonata . These studies only provided limited new insights into the phylogeny of Euthyneura. However, a sound phylogenetic hypothesis of a taxon is the prerequisite to reconstruct evolutionary changes in the group of interest.
To this end, we present an extensive phylogenetic analysis of the relationships of Euthyneura by using a multi-gene dataset including nuclear and mitochondrial genes. For the first time a broad taxon sampling of all major euthyneuran subgroups is considered. Based on the phylogenetic hypotheses proposed here, we discuss evolutionary trends within Euthyneura. In particular, we propose new hypotheses how invasion of freshwater and terrestrial habitats as major evolutionary events of the Gastropoda, has occurred.
Sequence alignment and statistical tests
Information on taxon sampling. Taxon names, localities, accession numbers provided (taxonomic classification in suprafamilial categories follows Bouchet et al. ); sequences generated in current study are marked with an asterisk; -: missing sequences
Salinator cf. fragilis
Phallomedusa solida (formerly Salinator solida)
New Zealand, Wellington
Chilina sp. 1
Chilina sp. 2
New Zealand, Waikato
New Zealand, Wellington
Pneumoderma cf. atlantica
Scotia Arc, Atlantic
Scotia Arc, Atlantic
Spain Mediterranean Sea
New Zealand, North Island
New Zealand, North Island
Information on sequence alignments of the different datasets and models of sequence evolution for Bayesian analyses
Gene region and taxon set
Number of taxa
Length of alignment (after removal of ambiguous positions)
Excluded nucleotide positions
Model of sequence evolution
18S rRNA (large taxon set)
α = 0.3539
pinv = 0.3977
18S rRNA (reduced taxon set)
α = 0.3202
pinv = 0.4220
28S rRNA (large taxon set)
α = 0.5337
pinv = 0.2449
28S rRNA (reduced taxon set)
α = 0.3905
pinv = 0.0889
16S rRNA (large taxon set)
α = 0.6450
pinv = 0.2421
16S rRNA (reduced taxon set)
α = 0.5878
pinv = 0.2173
CO1 (large taxon set)
α = 0.4170
pinv = 0.2722
CO1 (reduced taxon set)
α = 0.3975
pinv = 0.3077
CO1 (without 3rd codon position) (large taxon set)
3rd codon positions
α = 0.3496
pinv = 0.3879
CO1 (without 3rd codon position) (reduced taxon set)
3rd codon positions
α = 0.3435
pinv = 0.4759
Performance of the chi-square-test in PAUP yielded a homogeneous base composition in the 28S-alignments (P = 0.999). The 18S- (P = 0.001) and 16S- alignments (P = 0.007) showed heterogeneous base composition mainly due to the sequences of Nudipleura (Bathydoris clavigera, Dendronotus dalli, Pleurobranchus peroni, Tomthompsonia antarctica) (for 18S) and Orbitestellidae (for 16S). The COI-alignment showed heterogeneous base composition when using all three codon positions (P = 0.000). However, we identified substitution saturation in the third codon position (Iss 0.830 >Iss·c 0.771 for large taxon set; Iss 0.753 >Iss·c 0.737 for reduced taxon set) and subsequently removed third codon positions from the alignments. The P-value of the chi-square test then changed to 1.00 indicating homogeneous base composition for all taxa.
Additionally, we observed substitution saturation in the 16S-alignments (Iss 1.071 >Iss·c 0.784 for large taxon set; Iss 1.02 >Iss·c 0.750 for reduced taxon set).
The relative rate test revealed that evolutionary rates are different in the investigated taxa and genetic markers. This is especially true for the two ribosomal genes 18S rRNA and 28S rRNA, where the Nudipleura show extremely high Z-Scores (e. g. 18S: Dendronotus dalli vs Orbitestella sp. = 12.19; 28S: Dendronotus dalli vs Turbonilla sp. = 5.34).
Investigation of differences in incongruence length between the four different sequence partitions and two taxon sets revealed that combination of the partitions improves phylogenetic signal with a p value of 0.01 (10.000 replicates).
We performed different analyses which provided mostly congruent results regarding phylogenetic relationships of subgroups within Euthyneura.
The first striking result of this study is the inclusion of the taxon Turbonilla sp. (Pyramidelloidea) within Euthyneura as sister taxon to the Amphiboloidea (Figs. 1, 2 and 3). Within the Euthyneura Opisthobranchia are polyphyletic (Figs. 1 and 3) or paraphyletic (Fig. 2). The Opisthobranchia comprise several distinct clades. The most basal offshoot within Euthyneura is a clade composed of Acteonoidea and Nudipleura, both forming a sister group relationship. However, the internal branches leading towards Acteonoidea and Nudipleura are relatively long. Additionally, terminal branches of Bathydoris clavigera and especially Dendronotus dalli are extremely long indicating high substitution rates in these taxa. Therefore, this result should be considered with caution.
The second opisthobranch clade comprises the Cephalaspidea as sister to the Umbraculoidea plus Aplysiomorpha (Figs. 2 and 3). When Thecosomata and Gymnosomata are included (Fig. 1), they represent the immediate sister taxon to the Aplysiomorpha. The latter three taxa are sister to Umbraculoidea. The Sacoglossa are recovered closely related to the Pulmonata as sister to the Siphonariidae (Figs. 1 and 3). However, support for this sistergroup relationship is non existent in the analyses of the reduced dataset. Nevertheless, inclusion of Sacoglossa within Pulmonata is well supported in all analyses, rendering the Pulmonata paraphyletic. Inclusion of the enigmatic Acochlidiacea in the large taxon placed them within Pulmonata (Fig. 1).
Within Pulmonata we recovered monophyly for Eupulmonata. Whereas the Bayesian statistical support for a monophyletic Eupulmonata is rather low in the analysis of the large taxon set (Fig. 1) possibly due to missing data in these taxa (especially in Stylommatophora, see Table 1), the combined analyses of the reduced taxon set showed a high posterior probability (Fig. 2) and additionally high bootstrap support for a monophyletic Eupulmonata (Fig. 3). Within Eupulmonata, Onchidioidea are a well supported monophyletic clade whereas the Bayesian posterior probability for a monophyletic Stylommatophora is low in the analysis of the large taxon set probably due to the reasons mentioned above. Ellobioidea are poorly supported and their monophyly cannot be recovered unequivocally. Basommatophora are paraphyletic. Within Basommatophora, Siphonariidae and Hygrophila are well supported monophyletic taxa.
Two clades can be distinguished within Hygrophila. The first clade consists of Chilina spp. and Latia neritoides whereas the second clade is comprised of higher limnic Basommatophora. The relationships in this latter clade differ in the different analyses. Within higher limnic Basommatophora only a close relationship of Bulinus tropicus and Planorbis planorbis is consistent and well supported in all analyses.
Reconstruction of character evolution
Based on the phylogenetic hypothesis for Euthyneura deduced with a Bayesian analysis of the reduced data set we reconstructed the ancestral character states and character evolution relating to habitat types in euthyneuran subgroups.
With the current study we present a comprehensive molecular phylogenetic analysis of Euthyneura including representatives of all major subgroups. We used different approaches to infer a phylogenetic hypothesis for this taxon of Gastropoda. These approaches yielded for the most part constant results respective to the deeper nodes in the tree and indicated only minor differences at generic or familial level.
The unification of Opisthobranchia and Pulmonata in the group Euthyneura has been widely accepted since its original definition by Spengel . Consequently, the monophyly of Euthyneura has mostly been accepted in recent systematic and phylogenetic investigations [5, 9, 11–13, 15, 23, 24]. Although the defining character of Euthyneura, euthyneury, is considered to be a result of multiple convergence [16, 17], several autapomorphic characters supporting monophyly have been proposed for Euthyneura [11, 16, 17].
Our results contradict a monophyletic Euthyneura, since Pyramidelloidea are included within euthyneurans as sister group to the Amphiboloidea. This is in congruence with Grande et al.  who found Pyramidelloidea deeply nested within Pulmonata in their molecular phylogenetic analysis. Although not having included Pyramidelloidea in his molecular phylogenetic analysis of Euthyneura, Thollesson  proposed a possible synapomorphy of Pyramidelloidea and Euthyneura in the presence of a gap in the helix G16 of the 16S rRNA gene, thus supporting inclusion of Pyramidelloidea within Euthyneura as proposed here. Studies considering additional pyramidelloid and other basal heterobranch taxa currently undertaken in the group of the senior author will hopefully shed more light on the position of the enigmatic Pyramidelloidea within Heterobranchia (Euthyneura plus Heterostropha sensu Ponder and Waren ). Additionally, new character complexes, such as secondary structures of ribosomal genes, could provide further valuable phylogenetic information for the taxa in question, as has been demonstrated recently by Lydeard et al. .
Although the separation between Opisthobranchia and Pulmonata is rather distinct because of e. g. characters of the nervous system, it has been difficult to propose sound autapomorphies for a monophyletic Opisthobranchia . Although the subgroups of Opisthobranchia can be clearly distinguished and appear to be monophyletic [21, 26–29], monophyly of the Opisthobranchia altogether could not be shown by most recent phylogenetic analyses, regardless of whether they used morphological or molecular data [18, 19, 21, 22, 30]. The Opisthobranchia appear to be paraphyletic (cp. e. g. [7, 18]) rather than a natural grouping. Our data confirm this latter assumption by rendering the Opisthobranchia either polyphyletic or paraphyletic (depending on the analysis). Filtering our MCMC trees of the Bayesian analysis of the reduced dataset (including all genes) under the constraint of a monophyletic Opisthobranchia revealed that out of 100.000 trees not one single tree supports a monophyletic Opisthobranchia. Application of the Approximately Unbiased test  to both reduced datasets also rejected a monophyletic Opisthobranchia at the 5% significance level. In contrast, we can distinguish several clades within Opisthobranchia clearly distinct from each other gradually leading to the pulmonate level of organisation.
One such clade comprises the Nudipleura and Acteonoidea which in our trees appears at the base of the Euthyneura. Acteonoidea have traditionally been regarded as the most primitive opisthobranchs or have even been excluded from Opisthobranchia [22, 27, 32] whereas Nudipleura are derived [21, 22]. However, this unexpected grouping of both taxa has recently continuously been revealed by molecular systematic studies. [20, 21, 33]. Nevertheless, statistical support in our analyses is rather low and good synapomorphies for this sister group relationship are still warranted. Moreover, we observed deviant base composition and rate heterogeneity in Nudipleura which also could attribute to the basal position of this taxon and possibly artificially groups them with Acteonoidea.
A second well supported opisthobranch clade in the current analysis of the large taxon set comprises the Cephalaspidea, Umbraculoidea and Aplysiomorpha plus Thecosomata and Gymnosomata (Pteropoda). This close grouping of Cephalaspidea, Aplysiomorpha and Pteropoda has been suggested lately by several studies [17, 33]. The position of Umbraculoidea within this clade, although well supported in our current analyses, should be considered with caution since only one umbraculoid taxon has been included. Moreover, no morphological synapomorphies are known to support a close relationship of Umbraculoidea and Aplysiomorpha/Pteropoda. More umbraculoid taxa must be considered in future studies to clarify the phylogenetic position of this taxon.
The phylogenetic position of Sacoglossa within Opisthobranchia/Euthyneura has been a matter of debate [20–22]. Although morphologically well defined as a monophylum [26, 27], different analyses assign Sacoglossa equivocally to different clades within the Euthyneura. Our data suggest a close affinity to primitive Pulmonata. However, Sacoglossa are definitely a taxon that needs to be paid more attention to in future phylogenetic studies.
The same holds true for the Acochlidiacea, an enigmatic taxon inhabiting interstitial marine habitats and even freshwater systems, a feature unique within Opisthobranchia. Due to their very small size (often less than 1mm in length) hardly anything is known about their morphology and life history. Recent morphological investigations utilising modern computer generated 3-D reconstructions [34, 35] have shed some light on the anatomy of these enigmatic gastropods, however, more studies are warranted. The phylogenetic position of the Acochlidiacea is still unclarified. Vonnemann et al.  found them as a basal taxon within Opisthobranchia, while our results imply a position within Pulmonata.
The monophyly of Pulmonata is widely accepted on the basis of morphological characters [6, 7, 11, 13, 16]. In contrast, our data suggest paraphyly of Pulmonata due to the inclusion of Sacoglossa, Pyramidelloidea and possibly Acochlidiacea. If our phylogenetic hypothesis is correct, one must postulate that pulmonate autapomorphies (such as acquisition of a pneumostome and pulmonary vessels as well as the presence of a procerebrum and dorsal bodies) have secondarily been reduced in Sacoglossa, Acochlidiacea and Pyramidelloidea.
Within Pulmonata we can distinguish two monophyletic clades: Eupulmonata and Hygrophila. The unification of Siphonarioidea and Amphiboloidea in the Thalassophila based on morphological data  could not be confirmed by our data.
The Eupulmonata comprise a group of marine, semi-terrestrial and truly terrestrial gastropods showing a high diversity with regards to species number and life history characters. Monophyly of this taxon is strongly supported by Wade and Mordan  and the present results, however no morphological apomorphy is known to date [9, 10]. Relationships within Eupulmonata have been disputed in the past. Monophyly of Stylommatophora is undoubted [11, 13–15, 17, 37] and can be supported by several autapomorphies [11, 13]. Our results also show a monophyletic Stylommatophora. Haszprunar and Huber  proposed a close relationship of Ellobiidae, Trimusculidae and Stylommatophora on the basis of apomorphic characters of the nervous system, whereas Dayrat and Tillier [11, 12] consider Stylommatophora and Systellommatophora (Onchidioidea and Veronicelloidea) to be closely related. Neither of these two hypotheses are confirmed by our findings since we find a close relationship between ellobioid taxa, Otinoidea, Onchidioidea and Trimusculoidea. The phylogenetic status of the Ellobioidea cannot be conclusively clarified from our data since they appear to be paraphyletic in the two analyses utilising all sequence data. Exclusion of 16S-sequences and 3rd codon positions of COI-sequences, however, recovers them monophyletic.
The Basommatophora sensu Nordsieck  and Bouchet et al.  are paraphyletic in our analyses. No topology in our set of 100.000 MCMC trees of the Bayesian analysis of the reduced dataset supports a monophyletic Basommatophora. Moreover, testing an alternative tree topology with a constrained monophyletic Basommatophora with the Approximately Unbiased test  rejected monophyly of Basommatophora at the significance level of 5%. This is not surprising since the monophyly of Basommatophora has already been disputed by Hubendick . Within Basommatophora the Hygrophila are a well supported and defined group of pulmonates. Our results support earlier hypotheses of a common origin of these freshwater taxa [12, 13, 17, 40]. For the first time, however, crucial taxa like Latia are included in a molecular study. Within Hygrophila two clades can be distinguished; on the one hand the Chilinoidea (sensu Boss , including Chilinidae and Latiidae), and on the other the higher limnic Basommatophora. Chilina and Latia are sister-species, a relationship that has already been mentioned earlier [13, 39, 41]. Higher limnic Basommatophora can be divided into four well distinguishable families  while interfamilial relationships of these taxa vary in the current analyses thus rendering them beyond the scope of this study. These relationships are discussed in other publications [42, 43].
Former taxonomic classifications of Pulmonata united a group of primitive taxa (Ellobiidae, Otinidae, Amphibolidae, Siphonariidae, Chilinidae and Latiidae) in the Archaeopulmonata . Other authors followed this classification [6, 3, 45]. However, our results clearly indicate a paraphyletic nature of these taxa and therefore we propose the disuse of the name Archaeopulmonata.
Colonisation of freshwater and terrestrial habitats
One key step in the evolution of Euthyneura was the invasion of freshwater and terrestrial habitats by Pulmonata resulting in a multitude of taxa worldwide adapted to these habitats. It is undisputed that the first pulmonate gastropods were marine organisms [12, 46]. Marine pulmonates can be found in truly marine submersed environments (such as Williamia) and, more frequently, in upper littoral zones (Siphonaria, Myosotella, Trimusculus). Certain taxa even occur in estuaries or mangroves (Onchidiidae, certain Ellobiidae, Amphibolidae). Terrestrial pulmonates inhabit a wide spectrum of habitats, ranging from caves to lowlands to mountains and from boreal to tropical ecological zones. The same holds true for freshwater taxa, which occur in different types of limnic environments worldwide.
Our reconstruction of character evolution relating to habitat type (Fig. 4) shows that within Pulmonata the freshwater habitat has only been conquered once by the Hygrophila. Calculation of posterior probabilities for the different habitat types at specific nodes indicates that the ancestor of Hygrophila probably already lived in a freshwater habitat. Acknowledging that there are extremely few onchidioidean species reported to live in brackish or freshwater it would not change the picture that the major radiation into freshwater happened only once leading to the Hygrophila. The same is true for the few acochlidiaceans that are known from freshwater habitats. Freshwater members of both taxa were not included in our data set, for the time being, we would also hypothesize a similar evolutionary pathway excluding a terrestrial step.
In non-pulmonates, the situation is different. 'Prosobranchs', such as the superfamily Cerithoidea, comprise predominantly marine taxa. Nevertheless, freshwater was invaded independently in several cerithoid lineages . Even the evolution of life history traits such as viviparity in the freshwater invaders was correlated with this evolutionary step .
The ancestor of Eupulmonata and Hygrophila, in contrast, appears to have lived in a marginal zone like supralittoral zones, estuaries or mangroves. Therefore we conclude, that colonisation of freshwater in Pulmonata occurred via an aquatic pathway directly from the marine realm and not via a terrestrial step.
The terrestrial habitats have most probably also been invaded via marginal zones, as can be seen from the Bayesian reconstruction of ancestral character states in Eupulmonata. Involvement of freshwater systems in colonisation of terrestrial habitats is less likely, since the freshwater taxa (Hygrophila) are clearly separated from Eupulmonata and the posterior probability for a freshwater habitat is basically non-existant at the base of the Eupulmonata.
Terrestriality on the contrary is thought to have evolved much more than 10 times independently (discussed in Barker ). Barker  already emphasized that recent ellobiid terrestriality was clearly derived from marine littoral ancestors and not secondarily from terrestrial ancestors.
Invasion of marine habitats by Otina ovata within Eupulmonata and Turbonilla sp. obviously is the result of a secondary colonisation originating in marginal zones.
Euthyneura are considered the crown group of Gastropoda. Within this taxon Gastropoda have reached their peak in species richness and ecological diversity. This obvious evolutionary success can probably be attributed to several factors. Marine Opisthobranchia, e. g., have evolved several clades specialised on less utilised food resources such as sponges or cnidarians. A key innovation in the evolution of Pulmonata was the colonization of freshwater and terrestrial habitats.
Previously published phylogenies [11, 17, 19] did not allow an exact inference of possible pathways respective to pulmonate distribution. Up to now, it was not clear whether the invasion of freshwater happened directly from the marine environment or via a terrestrial step. Based on our phylogenetic hypothesis of Euthyneura and especially Pulmonata we are now able to trace an evolutionary scenario regarding colonisation of different habitats by these Gastropoda.
Our study on the phylogeny of Euthyneura has clearly shown, that traditional classification of these Gastropoda needs to be reconsidered. We were able to present well supported clades and reconstructed the evolutionary history of these clades regarding invasion of habitats and occupation of ecological niches. This should serve as the basis for future discussions on the evolution of this successful group of gastropod molluscs.
Taxon sampling and specimen collection
A total of 53 representative taxa of most major extant lineages of Euthyneura were included in the current phylogenetic study. Additionally, we included two basal heterobranch species (Turbonilla sp. and Orbitestella sp.) as well as the caenogastropod Littorina littorea. Specimens were collected from different locations worldwide (for details see Table 1). They were collected from the field by hand, snorkelling or scuba diving, preserved in 80–100% ethanol and stored cooled until further processing.
For some taxa (Stylommatophora, Amphibolidae, Ellobiidae, Opisthobranchia and Littorinidae) we obtained published sequences from Genbank). Accession numbers of all sequences used in the analyses are listed in Table 1.
DNA extraction, PCR and sequencing
Genomic DNA was extracted from muscle tissue or entire animals using the DNeasy Tissue Kit (Qiagen, Hilden, Germany) or the protocol given in . Voucher depositions are listed in Additional file 1. We amplified fragments of four genes, including 18S rDNA, 28S rDNA, 16S rDNA and COI. For details of primers used and PCR protocols see Additional file 2.
After the amplification products were purified from an agarose gel using the QIAquick Gel Extraction Kit from Qiagen (Hilden, Germany). The DNA was subjected to cycle sequencing using the ABI Prism Big Dye terminator kit (Perking-Elmer, Norwalk, CT, USA) or the CEQ DTCS Quick Start Kit (Krefeld, Germany). DNA sequences were obtained with an ABI 377 automated DNA sequencer at the Scientific Research Lab, Frankfurt/Main and by using a CEQ 2000 Beckmann Coulter sequencer. All fragments were sequenced in both directions using the same primer sequences as used for the PCR. For 18S and 28S several internal primers were used (Additional file 3).
Sequence alignments were performed with CLUSTAL W implemented in the software package BioEdit Version 7.0.5 . Default parameters were used but subsequent manual correction was necessary and was performed with BioEdit. Table 2 provides an overview on the number of taxa and lengths of the sequence alignments used in the analyses. Several base positions have been excluded from the alignments of 18S rRNA, 28S rRNA and 16S rRNA (for details see Table 2) prior to reconstructing phylogeny due to inserts in certain taxa or due to high variability within the alignments (especially in the 28S- and 16S-fragment).
We investigated base compositions by means of the software PAUP 4.0b10 . To test the degree of substitution saturation we used the test of Xia et al. , as implemented in the software package DAMBE . A relative rate test was performed with the software k2WuLi  in order to test for rate heterogeneity in the sequences. In order to examine whether there are significant differences in incongruence length between the four datasets, a homogeneity-partition test implemented in PAUP 4.0b10  was performed. Alternative tree topologies were tested for the reduced taxon sets using the Approximately Unbiased (AU) test . The likelihood at each nucleotide position was calculated for each alternative topology (constrained monophyletic Opisthobranchia, constrained monophyletic Basommatophora) as well as the topology under scrutiny using PAUP 4.0b10 . Likelihoods were used to calculate p-values using CONSEL version 0.1 .
Prior to phylogenetic analyses, we determined the optimal model of sequence evolution for each of the four partitions and each of the taxon sets using Modeltest 3.7  based on the Akaike information criterion (for details of the models determined refer to Table 2).
We applied several different analyses to our data. First, we used a concatenated alignment of all four partitions including all taxa for which sequences were available (large taxon sets). Missing sequences for certain taxa in certain partitions were coded as missing data in the respective alignments.
Secondly, we also followed a more conservative approach by analysing a combined dataset of all four partitions with only those taxa for which sequences of all partitions were available (reduced taxon set, 34 taxa). For all analyses Bayesian inference methods were applied with Mr Bayes 3_03b , using separate models of evolution for each of the four partions. The tree space was explored using four chains of a Markov Chain Monte Carlo algorithm (one cold, three heated) for 1,000000 generations. Likelihoods of the trees started at around -67470 and quickly converged upon a stable value of about -54600 after approximately 7,000 generations. Thus we computed a 50% majority rule consensus tree with the first 1,000 trees (= 10,000 generations) ignored as burn-in. Posterior probabilities were calculated for each node in the tree. We consider a BPP value of 0.95 and higher to give good support to a node.
Additional Maximum likelihood analyses were conducted for all data sets using RAxML-VI-HPC version 4.0.0 ). This program allows analyzing large combined data sets and uses a GTR model based approach with internal estimation of all free model parameters for all data partitions. Bootstrapping with 100 replicates was performed.
Trees were rooted with the caenogastropod Littorina littorea.
Reconstruction of character evolution
We reconstructed character evolution regarding habitat types with a Bayesian approach as implemented in the BayesTraits software package . The BayesMultiState option allows to automatically find posterior distribution of models of evolution using the reversible-jump MCMC method. Ancestral character states were reconstructed for selected nodes in the phylogeny resulting in respective probabilities of all character states for that node. Data on habitat types colonised by the investigated taxa were taken from the literature. Information on coding for individual taxa can be taken from Additional file 4. Rate deviation was set to 8.0. A hyperprior approach was used with exponential prior seeded from a uniform on the intervall 0 to 30. Thus, acceptance rates between 20 and 30 % were achieved, which are in the preferred range.
We are indebted to many colleagues who helped in collecting material from all over the world: Lydia Bousset (Thiverval-Grignon, FRA), the late David Brown (London, UK), Benoit Dayrat (Merced, USA), Andrzej Falniowski (Krakow, POL), Jeff Goddard (Santa Barbara, USA), Martin Haase (Greifwald, GER), Marc Hasenbank (Wellington, NZ), Alan Hodgson (Grahamstown, SA), Aslak Jørgensen (Charlottenlund, DK), Michael Schrödl and Enrico Schwabe (Munich, GER), Sid Staubach (Frankfurt, GER), Magda Szarowska (Krakow, POL), Richard Willan (Darwin, AUS), Tim Wollesen (Frankfurt, GER).
DNA samples and few sequences of Opisthobranchia were provided by Verena Vonnemann (Hamburg, GER). Claudia Nesselhauf (Frankfurt, GER) helped in sequencing some of the material.
Heiko Schmidt (Vienna) was helpful in applying the AU test.
We are thankful to Benoit Dayrat and three anonymous referees for valuable comments.
This project was funded by the German Science Foundation (KL 1303/1-3, KL 1303/4-1) and by the Hessian Ministry of Science and Culture to AKK and by the Freunde und Förderer of Frankfurt University to BS.
- Thiele J: Handbuch der systematischen Weichtierkunde. II. Subclassis Opisthobranchia. 1931, Jena: Gustav Fischer Verlag
- Fretter V, Graham A: British Prosobranch Molluscs. 1962, London: Ray Society
- Boss KJ: Mollusca. Synopsis and classification of living organisms. Edited by: Parker SP. 1982, New York: McGraw Hill Book Company, 945-1166.
- Spengel JW: Die Geruchsorgane und das Nervensystem der Mollusken. Z Wiss Zool. 1881, 35: 333-383.
- Ponder WF, Lindberg D: Towards a phylogeny of gastropod molluscs: an analysis using morphological characters. Zool J Linn Soc. 1997, 119: 83-265. 10.1006/zjls.1996.0066.View Article
- Tillier S: Relationships of gymnomorph gastropods (Mollusca: Gastropoda). Zool J Linn Soc. 1984, 82: 345-362.View Article
- Haszprunar G: The Heterobranchia – a new concept of the phylogeny of the higher Gastropoda. Z Zool Syst Evolutionsforsch. 1985, 23: 15-37.View Article
- Haszprunar G: On the origin and evolution of major gastropod groups, with special reference to the streptoneura. J Moll Stud. 1988, 54: 367-441. 10.1093/mollus/54.4.367.View Article
- Salvini-Plawen L, Steiner G: Synapomorphies and plesiomorphies in higher classification of Mollusca. Origin and evolutionary radiation of the Mollusca. Edited by: Taylor J. 1996, Oxford: Oxford University Press, 29-51.
- Ponder WF, Waren A: Classification of the Caenogastropoda and Heterostropha – a list of the family-group names and higher taxa. Malacol Rev. 1988, 4 (Suppl 4): 288-328.
- Dayrat B, Tillier S: Evolutionary relationships of euthyneuran gastropods (Mollusca): a cladistic re-evaluation of morphological characters. Zool J Linn Soc. 2002, 135: 403-470. 10.1046/j.1096-3642.2002.00018.x.View Article
- Dayrat B, Tillier S: Goals and limits of phylogenetics – The Euthyneuran Gastropods. Molecular Systematics and phyleogeography of molluscs. Edited by: Lydeard C, Lindberg D. 2003, Washington: Smithsonian Books, 161-184.
- Nordsieck H: Phylogeny and system of Pulmonata. Arch Moll. 1992, 121: 31-52.
- Tillier S, Masselot M, Tillier A: Phylogenetic relationships of the pulomante gastropods from rRNA sequences, and tempo and age of the stylommatophoran radiation. Origin and Evolutionary Radiation of the Mollusca. Edited by: Taylor J. 1996, Oxford: Oxford University Press, 267-284.
- Wade CM, Mordan PB: Evolution within the gastropod molluscs; using the ribosomal RNA gene-cluster as an indicator of phylogenetic relationships. J Moll Stud. 2000, 66: 565-570. 10.1093/mollus/66.4.565.View Article
- Haszprunar G, Huber G: On the central nervous system of Smeagolidae and Rhodopidae, two families questionably allied to the Gymnomorpha (Gastropoda, Euthyneura). J Zool. 1990, 220: 185-199.View Article
- Dayrat B, Tillier A, Lecointre G, Tillier S: New clades of euthyneuran Gastropods (Mollusca) from 28S rRNA sequences. Mol Phyl Evol. 2001, 19: 225-235. 10.1006/mpev.2001.0926.View Article
- Thollesson M: Phylogenetic analysis of Euthyneura (Gastropoda) by means of the 16S rRNA gene: use of a 'fast'gene for 'higher-level'phylogenies. Proc R Soc Lond B. 1999, 266: 75-83. 10.1098/rspb.1999.0606.View Article
- Grande C, Templado J, Cervera JL, Zardoya R: Molecular Phylogeny of Euthyneura (Mollusca:Gastropoda). Mol Biol Evol. 2004, 21: 303-313. 10.1093/molbev/msh016.View ArticlePubMed
- Grande C, Templado J, Cervera JL, Zardoya R: Phylogenetic relationships among Opisthobranchia (Mollusca: Gastropoda) based on mitochondrial cox 1, trnV, and rrnL genes. Mol Phyl Evol. 2004, 33: 378-388. 10.1016/j.ympev.2004.06.008.View Article
- Vonnemann V, Schrödl M, Klussmann-Kolb A, Wägele H: Reconstruction of the phylogeny of the Opisthobranchia (Mollusca, Gastropoda) by means of 18S and 28S rRNA gene sequences. J Moll Stud. 2005, 71: 113-125. 10.1093/mollus/eyi014.View Article
- Wägele H, Klussmann-Kolb A: Opisthobranchia (Mollusca, Gastropoda) - more than just slimy slugs. Shell reduction and its implications on defence and foraging. Front Zool. 2005, 2 (1): 3-10.1186/1742-9994-2-3.PubMed CentralView ArticlePubMed
- Tillier S, Masselot M, Guerdoux J, Tillier A: Monophyly of major gastropod taxa tested from partial 28S rRNA sequences, with emphasis on Euthyneura and Hot-Vent Limpets Peltosporoidea. Nautilus. 1994, 122-140. Suppl 2
- Yoon SH, Kim W: Phylogeny of some gastropod molluscs derived from 18S rRNA sequences with emphasis on Euthyneura. Nautilus. 2000, 114: 84-92.
- Lydeard C, Holznagel WE, Ueshima R, Kurabayashi A: Systematic implications of extreme loss or reduction of mitochondrial LSU rRNA helical-loop structure in gastropods. Malacologia. 2002, 44: 349-352.
- Jensen KR: Phylogenetic systematics and classification of the Sacoglossa (Mollusca, Gastropoda, Opisthobranchia). Phil Trans R Soc Lond B. 1996, 351: 91-122. 10.1098/rstb.1996.0006.View Article
- Mikkelsen PM: The evolutionary relationships of Cephalaspidea s. l. (Gastropoda: Opisthobranchia): a phylogenetic analysis. Malacologia. 1996, 37: 375-442.
- Wägele H, Willan R: Phylogeny of the Nudibranchia. Zool J Linn Soc. 2000, 130: 83-181. 10.1006/zjls.1999.0214.View Article
- Medina M, Walsh PJ: Molecular systematics of the order Anaspidea based on mitochondrial DNA sequences (12S, 16S, and COI). Mol Phyl Evol. 2000, 15: 41-58. 10.1006/mpev.1999.0736.View Article
- Wägele H, Vonnemann V, Wägele W: Toward a phylogeny of the Opisthobranchia. Molecular Systematics and Phylogeography of Mollusks. Edited by: Lydeard C, Lindberg DR. 2003, Washington: Smithsonian Books, 185-228.
- Shimodaira H: An Approximately Unbiased Test of Phylogenetic Tree Selection. Syst Biol. 2002, 51: 492-508. 10.1080/10635150290069913.View ArticlePubMed
- Mikkelsen PM: Shelled opisthobranchs. Adv Mar Biol. 2002, 42: 67-136.View ArticlePubMed
- Klussmann-Kolb A, Dinapoli A: Systematic position of the pelagic Thecosomata and Gymnosomata within Opisthobranchia (Mollusca, Gastropoda) – revival of the Pteropoda. J Zool Syst Evol Res. 2006, 44: 118-129. 10.1111/j.1439-0469.2006.00351.x.View Article
- Sommerfeldt N, Schrödl M: Microanatomy of Hedylopsis ballantinei, a new interstitial acochlidian gastropod from the Red Sea, and its significance for phylogeny. J Moll Stud. 2005, 71: 153-165. 10.1093/mollus/eyi018.View Article
- Neusser TP, Heß M, Haszprunar G, Schrödl M: Computer-based three-dimensional reconstruction of the anatomy of Microhedyle remanei (Marcus, 1953), an interstitial acochlidian gastropod from Bermuda. J Morph. 2006, 267: 231-247. 10.1002/jmor.10398.View ArticlePubMed
- Knudsen B, Kohn AB, Nahir B, MacFadden CS, Moroz LL: Complete DNA sequence of the mitochondrial genome of the sea-slug, Aplysia californica : Conservation of the gene order in Euthyneura. Mol Phyl Evol. 2006, 38: 459-469. 10.1016/j.ympev.2005.08.017.View Article
- Wade CM, Mordan PB, Naggs F: Evolutionary relationships among the pulmonate land snails and slugs (Pulmonata, Stylommatophora). Biol J Linn Soc. 2006, 87: 593-610. 10.1111/j.1095-8312.2006.00596.x.View Article
- Bouchet P, Rocroi P, Fryda J, Hausdorf B, Ponder W, Valdes A, Waren A: Classification and Nomenclator of Gastropod Families. Malacologia. 2005, 47: 1-397.
- Hubendick B: Systematics and comparative morphology of the Basommatophora. Pulmonates. Systematics, evolution and ecology. Edited by: Fretter V, Peake J. 1978, New York, London, San Francisco: Academic Press, 24: 1-47.
- Salvini-Plawen L: Origin, phylogeny and classification of the phylum Mollusca. Iberus. 1990, 9: 1-33.
- Vaught KC: A classification of the living Mollusca. 1989, Melbourne, F.L : American Malacologists, Inc
- Albrecht C, Wilke T, Kuhn K, Streit B: Convergent evolution of shell shape in freshwater limpets: the African genus Burnupia. Zool J Linn Soc. 2004, 140: 577-588. 10.1111/j.1096-3642.2003.00108.x.View Article
- Albrecht C, Kuhn K, Streit B: A molecular phylogeny of Planorboidea (Gastropoda: Pulmonata): insights from enhanced taxon sampling. Zool Scr. 2007, 36: 27-39. 10.1111/j.1463-6409.2006.00258.x.View Article
- Morton JE: The evolution of the Ellobiidae with a discussion on the origin of the Pulmonata. Proc Zool Soc Lond. 1955, 125: 127-168.View Article
- Harbeck K: Die Evolution der Archaeopulmonata. Zoo Verh Leiden. 1996, 305: 3-133.
- Hubendick B: Phylogenie und Tiergeographie der Siphonariidae. Zur Kenntnis der Phylogenie in der Ordnung Basommatophora und des Ursprungs der Pulmonatengruppe. Zool Bid Upp. 1945, 24: 1-216.
- Lydeard C, Holznagel WE, Glaubrecht M, Ponder WF: Molecular phylogeny and evidence for multiple origins of freshwater gastropods of the circum-global, diverse superfamily Cerithioidea (Mollusca: Caenogastropoda). Mol Phyl Evol. 2002, 22: 399-406. 10.1006/mpev.2001.1072.View Article
- Köhler F, von Rintelen, Meyer A, Glaubrecht M: Multiple origin of viviparity in Southeast Asian gastropods (Cerithioidea: Pachychilidae) and its evolutionary implications. Evolution. 2004, 58: 2215-2226.View ArticlePubMed
- Barker GM: Gastropods on Land: Phylogeny, Diversity and Adaptive Morphology. I. The biology of terrestrial Mollusca. Edited by: Barker GM. 2001, Oxford: CABI Publishing, 1-146.View Article
- Hall TA: BioEdit: a user-friendly biological sequences alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser. 1999, 41: 95-98.
- Swofford DL: PAUP. Phylogenetic analysis using parsimony (and other methods). Version 4_10b. Sunderland, Mass: Sinauer Associates. 2002
- Xia X, Xie Z, Salemi M, Chen L, Wang Y: An index of substitution saturation and its application. Mol Phyl Evol. 2003, 26: 1-7. 10.1016/S1055-7903(02)00326-3.View Article
- Xia X, Xie Z: DAMBE: data analysis in molecular biology and evolution. J Hered. 2001, 92: 371-373. 10.1093/jhered/92.4.371.View ArticlePubMed
- Wu CI, Li WH: Evidence for higher rates of nucleotides substitution in rodents than in man. Proc Nat Acad Sci. 1985, 82: 1741-1745. 10.1073/pnas.82.6.1741.PubMed CentralView ArticlePubMed
- Shimodaira H, Hasegawa M: CONSEL: for assessing the confidence of phylogenetic tree selection. Bioinformatics. 2001, 17: 1246-1247. 10.1093/bioinformatics/17.12.1246.View ArticlePubMed
- Posada D, Crandall KA: Modeltest: testing the model of DNA substitution. Bioinformatics. 1998, 14: 817-818. 10.1093/bioinformatics/14.9.817.View ArticlePubMed
- Huelsenbeck JP, Ronquist F: MrBAYES; Bayesian inference for phylogeny. Bioinformatics. 2001, 17: 754-755. 10.1093/bioinformatics/17.8.754.View ArticlePubMed
- Stammatakis A: RaxML-VI-HPC: Maximum Likelihood-based Phylogenetic Analyses with Thousands of Taxa and Mixed Models. Bioinformatics. 2006, 22: 2688-2690. 10.1093/bioinformatics/btl446.View Article
- Pagel M, Meade A: Bayesian analysis of correlated evolution of discrete characters by reversible-jump Markov chain Monte Carlo. Am Nat. 2006, 147: 808-825. 10.1086/503444.View Article
- Wollscheid E, Wägele H: Initial results on the molecular phylogeny of the Nudibranchia (Gastropoda, Opisthobranchia) based on 18S rDNA data. Mol Phyl Evol. 1999, 13: 215-226. 10.1006/mpev.1999.0664.View Article
- Winnepenninckx B, Backeljau T, De Wachter R: Small ribosomal subunit RNA and the phylogeny of Mollusca. Nautilus. 1994, 98-110. Suppl 2
- Bargues MD, Mas-Coma S: Phylogenetic analysis of lymnaeid snails based on 18S rDNA sequences. Mol Biol Evol. 1997, 14: 569-577.View ArticlePubMed
- Simon S, Frati F, Beckenbach A, Crespi B, Li H, Flook P: Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Ent Soc Am. 1994, 87: 651-701.View Article
- Schwenk K, Sand A, Boersma M, Brehm M, Mader E, Offerhaus D, Spaak P: Genetic markers, genealogies and biogeographic patterns in the Cladocera. Aqu Ecol. 1998, 32: 37-51. 10.1023/A:1009939901198.View Article
- Folmer O, Black M, Heah W, Lutz R, Vrijenhoek R: DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994, 3: 294-299.PubMed
- Stothard JR, Rollinson D: Partial DNA sequences from the mitochondrial cytochrome oxidase subunit I (COI) gene can differentiate the intermediate snail hosts Bulinus globosus und B. nasutus (Gastropoda: Planorbidae). J Nat Hist. 1997, 31: 727-737. 10.1080/00222939700770361.View Article
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