Cyanobacterial contribution to the genomes of the plastid-lacking protists
© Maruyama et al; licensee BioMed Central Ltd. 2009
Received: 13 March 2009
Accepted: 11 August 2009
Published: 11 August 2009
Eukaryotic genes with cyanobacterial ancestry in plastid-lacking protists have been regarded as important evolutionary markers implicating the presence of plastids in the early evolution of eukaryotes. Although recent genomic surveys demonstrated the presence of cyanobacterial and algal ancestry genes in the genomes of plastid-lacking protists, comparative analyses on the origin and distribution of those genes are still limited.
We identified 12 gene families with cyanobacterial ancestry in the genomes of a taxonomically wide range of plastid-lacking eukaryotes (Phytophthora [Chromalveolata], Naegleria [Excavata], Dictyostelium [Amoebozoa], Saccharomyces and Monosiga [Opisthokonta]) using a novel phylogenetic pipeline. The eukaryotic gene clades with cyanobacterial ancestry were mostly composed of genes from bikonts (Archaeplastida, Chromalveolata, Rhizaria and Excavata). We failed to find genes with cyanobacterial ancestry in Saccharomyces and Dictyostelium, except for a photorespiratory enzyme conserved among fungi. Meanwhile, we found several Monosiga genes with cyanobacterial ancestry, which were unrelated to other Opisthokonta genes.
Our data demonstrate that a considerable number of genes with cyanobacterial ancestry have contributed to the genome composition of the plastid-lacking protists, especially bikonts. The origins of those genes might be due to lateral gene transfer events, or an ancient primary or secondary endosymbiosis before the diversification of bikonts. Our data also show that all genes identified in this study constitute multi-gene families with punctate distribution among eukaryotes, suggesting that the transferred genes could have survived through rounds of gene family expansion and differential reduction.
Cyanobacterial ancestors gave rise to plastids (chloroplasts) in the ancestor of a eukaryotic lineage. The birth of the plastid had an impact on eukaryotic genome evolution, by way of endosymbiotic gene transfer (EGT), a particular form of lateral gene transfer (LGT) from endosymbionts into the phylogenetically discontiguous host genome . Subsequently, an algal ancestor gave rise to secondary plastids in several punctate lineages of eukaryotes. A number of these secondarily phototrophic lineages lost their photosynthetic ability and further diverged into secondarily heterotrophic, plastid-lacking protists [2, 3].
Chromalveolata is a large taxonomic group of eukaryotes, encompassing secondary phototrophs and secondarily heterotrophic protists , and the 'chromalveolate hypothesis' argues that this group originated from a common ancestor harboring the chlorophyll c-containing secondary plastid derived from a red alga (Figure 1) . Among the secondarily heterotrophic chromalveolates, several lineages have retained remnant chloroplasts for non-photosynthetic metabolic pathways, e.g. apicoplasts in apicomplexan parasites . Recent genomic surveys revealed the presence of plastid-derived genes, and further suggested the presence of cryptic secondary plastids in non-photosynthetic alveolate protists [25, 26]. Furthermore, re-examination of the whole genome sequences suggested the existence of algal genes in ciliates, another plastid-lacking alveolate lineage, which could support the photosynthetic ancestry of ciliates . Oomycetes are plastid-lacking stramenopiles, or chromists, classified into Chromalveolata . Although whole genome sequence analysis showed that a number of genes with affinity to photosynthetic organisms (cyanobacteria and algae) are encoded in the nuclear genome, most of these 'plastid imprints' candidates were only suggested by similarity search and phylogenetic analyses have not yet led to fully recovering the expected tree topology . Considering the uncertain phylogenetic affinity of the 'best hit' in similarity search , reassessment of the genome information is important to determine whether the evolutionary history of oomycetes is comparable to ciliates .
One candidate of 'plastid imprints' in oomycetes has been confirmed by studies reporting the phylogeny of gnd genes, which encode 6-phosphogluconate dehydrogenase, showing that some plastid-lacking protists have plant-like, cyanobacterium-derived gnd genes [20, 21, 30]. These analyses suggested that the gnd genes with cyanobacterial ancestry were acquired early in eukaryotic evolution, either via ancient eukaryote-to-eukaryote LGT, or primary EGT that occurred earlier than had ever been thought . Additionally, the phylogeny of gnd genes demonstrated that cyanobacterial genes are also present in several Excavata protists, e.g. the heterolobosean amoebo-flagellate Naegleria gruberi. Naegleria gruberi is a non-parasitic heterotrophic species related to N. fowleri, which is the causative agent of primary amoebic meningoencephalitis in mammals . Although the phylogenetic relationship within Excavata is still unclear, Heterolobosea, together with Jakobida, is likely to be a sister group of Euglenozoa [18, 32].
To address how many genes have cyanobacterial ancestry in plastid-lacking protists, and whether cyanobacterial ancestry is limited to this gnd gene or also found in other genes, we conducted a phylogenomic analysis using genome sequence data of a taxonomically wide range of plastid-lacking eukaryotes. Here we present a gene mining study with a novel pipeline automatically producing and summarizing one-by-one phylogenetic trees, and show phylogenetic analyses of resultant candidate genes with cyanobacterial ancestry, using the whole genome sequence data from a wide range of eukaryotic lineages.
Summary of eukaryote-type genes with cyanobacterial ancestry identified in this study
Uroporphyrin III methyltransferase
Cobalamin-independent methionine synthase
Amino acid decarboxylase
Cobalamin synthesis protein
Amino acid aminotransferase
Glyoxalase I family protein-like
We found that Uroporphyrin III methyltransferase gene homologs (Figure 2) consisted of two large subfamilies of genes with cyanobacterial ancestry, and that oomycete genes were included only in one of them. Given that both subfamilies include green plants, red algae, chromalveolates and cyanobacteria, it is likely that they diverged within the ancestral cyanobacteria and transferred into eukaryotic hosts via primary and secondary endosymbioses. Both of the subfamilies were concurrently present in the cyanobacterial and green algal genomes. In land plants, red algae, diatoms, haptophytes and the plastid-lacking oomycetes, one of the subfamilies might be lost along with the loss of the plastid. The Thalassiosira homolog formed a monophyletic group with green plants, rather than red algae, suggesting that it was acquired independently of the secondary plastid of the red lineage. In this study, the bacteriovorous choanoflagellate Monosiga brevicollis gene and the proteobacterial genes (Gluconobacter, Alteromonas and Nitrosomonas) were treated as 'apparently LGT-derived genes', incongruously showing affinities to photosynthetic bikonts .
Genes encoding cobalamin-independent methionine synthase in green and red algae, diatoms, and oomycetes formed a monophyletic group with cyanobacterial homologs, while the land plants and the red alga Cyanidioschyzon homologs were placed in different clades unrelated to cyanobacteria (Figure 3). Close association between diatom and oomycete genes suggested the deep ancestry of the genes in the chromalveolate lineage. We failed to find the homologs in the prasinophytes Ostreococcus and Micromonas, suggesting that this gene family was dispensable in some plant lineages.
One of the genes with cyanobacterial ancestry found in N. gruberi is pyridoxal-dependent amino acid decarboxylase gene (Figure 4). The tree indicated that green plants were split into different eukaryotic clades. Naegleria and chromalveolate genes showed robust monophyly with green plants, included in a cyanobacterial gene clade. The tree showed that land plants possessed another subfamily, associated with red algal and fungal genes, apparently of non-cyanobacterial origin. We also identified genes with cyanobacterial ancestry from Naegleria in an oxidoreductase gene family that included genes encoding Rieske iron-sulfur cluster 55 kDa protein of chloroplast inner membrane translocon (TIC55), chlorophyll a oxidase (CAO), Lethal-leaf spot 1 (LLS1, which is synonymous with pheophorbide a oxygenase (PAO)) and accelerated cell death 1 (ACD1) (Figure 5) [34, 35]. All the members of this family in land plants were hypothesized to be located at the inner membrane of the chloroplast, and to be involved in chlorophyll metabolism . The phylogenetic tree of the TIC55-like gene family showed intricate distribution of cyanobacterial, green plant and chromalveolate genes.
In other trees of the genes identified in this study (Additional files 2, 3, 4, 5, 6, 7, 8 and 9), gene clades with cyanobacterial ancestry were mostly composed of bikonts genes, besides the choanoflagellate M. brevicollis genes (see Discussion).
We identified eight and seven genes with cyanobacterial ancestry in the genome sequences of the oomycete P. ramorum and the heterolobosean N. gruberi, respectively (Table 1). It was reported that the apicomplexan Cryptosporidium 'recently' lost their secondary plastid, and retained two to seven putative plastid-derived genes in the genome . This number is comparable to our result of the gene mining study using oomycete and heterolobosean genomes. In addition, our system resolved the hidden diversity of the gene family repertoire in eukaryotic genomes by one-by-one gene phylogenies.
Secondary EGT scenario
Although the phylogenetic positions of Cryptophyceae and Haptophyta are still debatable [e.g. [17, 37–41]], the chromalveolate hypothesis has been reinstated to support the evolutionary scenario that the plastid-lacking protists oomycetes and ciliates once might have had a plastid . According to this hypothesis, the genes with cyanobacterial ancestry found in the oomycete genomes were acquired via secondary EGT in the common ancestor of Chromalveolata, from the red algal ancestor of secondary plastids. This explanation is also applicable under the alternative hypothesis for chromalveolate plastids, which proposes that a tertiary endosymbiont of the haptophyte/cryptophyceae lineage is the origin of the stramenopile/alveolate plastids . The phylogenetic tree of the photorespiratory glycerate kinase genes, suggesting the red algal origin of the Phytophthora genes (Additional file 7), is consistent with the chromalveolate hypothesis. However, several other gene trees in this study showed oomycete genes with green lineage affinity, not red algae (e.g. Additional files 2, 3 &4). Recently, Frommolt et al.  demonstrated that, out of 16 genes involved in carotenoid biosynthesis from chromalveolate algae, one third (5/16) of plastid-targeted, nuclear-encoded genes are most closely related to green algal homologs. Reyes-Prieto, Moustafa and Bhattacharya  identified 16 genes of possible algal origin in the ciliates Tetrahymena thermophila and Paramecium tetraurelia, and 7/16 of their trees show a close relationship between green plants and Chromalveolata. Frommolt et al.  attributed the close relationships between green plants and chromalveolate genes to the secondary endosymbiosis of an ancient green plant (e.g. prasinophyte), based on the hypothesis on the monophyly of the Archaeplastida [16, 40]. This explanation might be also applicable to the plant-like genes in ciliates .
While Heterolobosea and Euglenozoa are often united as the morphologically defined taxon, Discicristata, within Excavata , recent morphological and molecular phylogenetic analyses suggest that the heteroloboseans (e.g. Naegleria) never possessed the secondary plastid of green lineage and share the same origin with Euglenida . Molecular phylogenetic analyses showed that Excavata is separated from other secondary plastid-containing eukaryotes (Chromalveolata and Rhizaria) [18, 40]. Therefore, it is unlikely that the genes with cyanobacterial ancestry found in the heterolobosean nuclear genomes originated from the plastid cognate with any known secondary plastids in extant photosynthetic eukaryotes. The amino acid decarboxylase gene (Figure 4) and the gnd gene (Additional file 3)  trees demonstrated the presence of genes with cyanobacterial ancestry in other heterolobosean species than N. gruberi, suggesting that the ancestor of the genus Naegleria possessed this gene family. Furthermore, although ML bootstrap support or Bayesian posterior probability (BI) values were not always sufficient, the Naegleria genes occupy relatively basal phylogenetic positions within the bikonts clade in all seven trees (Figures 4 and 5; Additional files 3, 4, 6, 8, and 9). Thus it is possible that the genes with cyanobacterial ancestry were introduced en bloc in the ancestor of Heterolobosea, via a batch gene transfer, in a concerted manner. One possible origin of such a concerted gene transfer is secondary EGT from a photosynthetic eukaryote with a basal phylogenetic position within bikonts. However, as discussed above, it is unlikely that Heterolobosea experienced secondary endosymbiosis and acquired genes common to the extant secondary plastid-containing eukaryotes via secondary EGT.
Ancient eukaryote-to-eukaryote LGT or primary EGT scenarios
Recently, a hypothesis for the non-monophyly of Archaeplastida was proposed based on the phylogenetic analyses of slowly evolving nuclear-encoded genes [17, 19]. This non-monophyly hypothesis could be also considered within the scope of the primary EGT scenario. It is notable that a number of the trees in this study (Figure 2; Additional files 2, 3, 4, 6, and 8) showed intriguing topologies, depicting the split of Archaeplastida and inclusion of Chromalveolata and Excavata genes within it, as shown in the previously reported multiple slowly-evolving gene phylogeny  and gnd gene phylogeny [20, 21]. These results are consistent with the hypothesis for the non-monophyly of Archaeplastida, and suggest that the oomycete and heterolobosean genes with cyanobacterial ancestry might reflect the host nuclear genome phylogeny. On the other hand, the genes found in the marine choanoflagellate M. brevicollis were positioned within the bikonts clade, but not associated with the genes from other Opisthokonta relatives (Metazoa and fungi), suggesting that the tree topologies were probably not reflective of the host phylogeny  but eukaryote-to-eukaryote LGT (Figure 2; Additional files 4, 8, and 9). No gene with cyanobacterial ancestry was found in D. discoideum (Amoebozoa), and only one gene in S. cerevisiae (Opisthokonta). These results are also consistent with the ancient primary EGT scenario.
A photorespiratory gene with cyanobacterial ancestry in fungi
Our analysis using the genome data of the budding yeast S. cerevisiae identified one gene with cyanobacterial ancestry, encoding the glycerate kinase for photorespiration (Additional file 7). Given that photorespiration is essential for cyanobacteria and plants, it is likely that the glycerate kinases in plants and cyanobacteria are phylogenetically and physiologically related to photorespiration [47, 48]. A previous study on glycerate kinases showed that, regardless of the complete absence of photorespiratory metabolism in fungi, the gene product from the budding yeast Saccharomyces showed similar enzymatic activity and substrate specificity compared with the Arabidopsis gene, suggesting that the plant and fungal genes catalyze the same reaction in different contexts of the metabolic pathway . Another example of plant-type genes in fungi was reported in a phylogenetic study of the genes encoding high-affinity nitrate transporter NRT2, which suggested that fungi probably acquired the NRT2 genes via LGT from one of the chromalveolate lineages . Meanwhile, our data showed that the fungal clade was located outside the clade of plants plus oomycetes (Additional file 7), suggesting that fungal glycerate kinase genes with cyanobacterial ancestry likely originated from an LGT event from an ancestor of cyanobacteria, or eukaryote-to-eukaryote LGT from an ancestor of Archaeplastida (or bikonts). One likely explanation for the presence of photorespiratory genes in oomycetes is that the ancestor of Chromalveolata possessed this gene family, but some photosynthetic descendants lost this gene family or replaced it with other genes during the course of lineage-dependent customization of photorespiratory pathways [[50, 51]; for discussion on carbon assimilation in diatoms], while oomycetes retained the genes without any replacement.
Gene family expansion and differential reduction
Another conclusion of this analysis is that rounds of gene family expansion and selective reduction are important factors in making eukaryotic genome phylogeny look like a complicated mosaic (Figure 6). It is likely that the alteration of gene family repertoire contributed to the restructuring of the intracellular metabolome and a reduction of the dispensable gene families. Our data showed that all the genes identified in this study were members of multiple gene families. Algae and plastid-lacking protists retained only members of subfamilies (e.g. Figure 2 and Additional file 8), suggesting that the punctate distribution might be a corollary of the common mechanism by which genes with cyanobacterial ancestry were retained in their genomes. The presence of genes from multiple subfamilies in one organism supports this idea (e.g. two Uroporphyrin III methyltransferase subfamilies in prasinophytes and Volvox in Figure 2). Discontinuous loss or gain of a metabolic pathway in a lineage might be another factor in punctate distribution; e.g. the oxidative pentose pathway, and the cyanobacterial gnd genes functioning therein, were present in most bikonts but lost in the ciliate Tetrahymena [21, 52]. A recent study on pyridoxal-dependent amino acid aminotransferase reported that, besides the ancestrally eukaryotic enzymes, land plants possess a distinct subfamily of prokaryote-type chloroplast-targeted enzymes . Our data with richer taxon sampling identified another prokaryote-type subfamily with cyanobacterial ancestry (Additional file 8), illustrating the hidden evolutionary diversity of protist and algal metabolomes.
Our results showed that many genes with cyanobacterial ancestry identified in this study were found only in complete genome sequences, suggesting that these genes might be difficult to discover by expressed sequence tag (EST) library sequencing, probably due to the low-level expression of these genes. Although the whole genome data from excavate parasites (e.g. Trypanosoma, Giardia and Trichomonas) are available, they seem to be unsuited for the gene mining study because of the unusual nucleotide substitutions (see Methods). At the stage of starting the present gene mining study, N. gruberi was the only species with whole genome data released within the non-parasitic excavates, and thereby the excavate genes with cyanobacterial ancestry were mostly from N. gruberi. More genome data from plastid-lacking protists from Excavata and Rhizaria as well as Archaeplastida, especially red algae and glaucophytes, are needed to unravel the evolutionary history of plastids, and plastid-lacking protists.
The comparative analyses of the genome sequence data of the plastid-lacking eukaryotes demonstrated the potentially significant contributions of ancestral or extant cyanobacteria to the eukaryotic genomes, which probably occurred via LGT or ancient primary EGT events. Furthermore, the automated phylogenetic analyses revealed the diversity and punctate distribution of gene families within the genomes in the unicellular microbes. More genome data of the plastid-lacking Excavata and Rhizaria will make the evolutionary history clear and support our hypotheses.
The genome sequence data of P. ramorum, N. gruberi and M. brevicollis was produced by the US Department of Energy Joint Genome Institute (JGI) . D. discoideum genome data (9 Nov 2007) at dictyBase  and S. cerevisiae genome data  were used for phylogenetic analysis. Red algal data were retrieved from the Cyanidioschyzon merolae , Galdieria sulphuraria  genome databases, and other algal data were from Aureococcus anophagefferens, Emiliania huxleyi, Micromonas pusilla, Micromonas sp. RCC299, Ostreococcus tauri, Ostreococcus sp. RCC809, Phaeodactylum tricornutum, Phytophthora sojae, Thalassiosira pseudonana and Volvox carteri genome databases on JGI. EST sequences of several protists were obtained from TBestDB  and all other sequences were from the NCBI GenBank refseq database . We excluded amitochondrial and/or parasitic eukaryotes, which might cause long branch attraction due to unusual nucleotide substitutions [61, 62]. Fragments of N. fowleri amino acid decarboxylase gene [DDBJ: AB491948] were amplified from genomic DNA using degenerated primers based on the conserved amino acid motif YHHFGYP for the forward primer (TAYCAYCAYTTIGGITAYCC) and WQLACEG for the reverse primer (CCYTCRCAIGCIARYTGCCA). PCR products were directly sequenced using an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) with a BigDye Terminator Cycle Sequencing Ready Reaction kit v. 3.1 (Applied Biosystems).
A genome-wide phylogenetic program was made with several bench-made BioRuby scripts (Additional file 1), referring to the previously reported phylogenomic pipeline used in the macronuclear genome analysis of Tetrahymena thermophila . For the first screening, query amino acid sequences were automatically subjected to BLAST searching using NCBI netblast  and EFetch utilities , extracting the genes showing the highest E-value to a cyanobacterial counterpart among 'Bacteria' by BLASTP. For the second step, these genes were subjected to BLASTP analysis against 'refseq-protein' to fetch homologous sequences with E-values less than 0.001, up to 500 hits at a maximum. Multiple alignments were then performed using MUSCLE , which automatically removed ambiguously aligned sites or sequences with too many gaps. Bootstrapped neighbor-joining trees were produced using QuickTree . Trees were output in the PostScript format using the newicktops program in the NJplot package  with sizes and colors of OTU names modified according to the NCBI taxonomy database  to simplify the subsequent visual checking process. Genomes of several bacterial genera were intensively sequenced and many homologous sequences from closely related species and strains (e.g. Escherichia, Bacillus) appeared on the trees. To diminish the sampling bias, the output files of QuickTree were also used to parse tree topology and detect a monophyletic clade exclusively composed of OTUs from a single genus using Bio::Tree class methods in BioRuby scripts. One representative OTU was automatically selected in such single-genus clades, the other OTUs were removed, and the trees were re-constructed for visual checking. In addition to the automatic process, trees for genes listed in the putative photosynthetic endosymbiont-derived genes , but not detected in our analysis, were manually re-constructed. Non-cyanobacterial prokaryotic genes taxonomically unrelated to, but placed within, the cyanobacterial clade were interpreted as 'apparently LGT-derived genes' with cyanobacterial ancestry.
Candidate cyanobacteria-related genes were manually selected, their homologs were collected from major groups of the three domains of life, and then subjected to multiple protein sequence alignments using MUSCLE. Phylogenetic analyses were performed with a maximum likelihood (ML) method using RAxML  and with a Bayesian interference (BI) method using MrBayes . ML and BI were based on the WAG substitution matrix with options of four gamma-distributed rate categories and estimate of invariable sites (plus empirical base frequencies in ML). ML branch support was evaluated with 1000 bootstrap replicates, and BI posterior probability values were calculated from the MCMC run data, which summarized when the average standard deviation of split frequencies reached less than 0.01. Except for cyanobacterial genes of which no homologs were found in other prokaryotes (e.g. Additional file 2), or of which monophyly was confirmed by previous studies (e.g. Additional file 3), threshold values to assess the monophyly of cyanobacterial gene clades were 50% on ML bootstrap or 0.9 on BI posterior probability values.
Bayesian posterior probability
endosymbiotic gene transfer
expressed sequence tag
lateral gene transfer
Operational Taxonomic Unit
Rieske iron-sulfur cluster 55 kDa protein of chloroplast inner membrane translocon.
We thank Dr. Kenji Yagita and Dr. Takuro Endo for kindly providing the genomic DNA from N. fowleri strain Nf 66. Computation time was provided by the Super Computer System, Human Genome Center, Institute of Medical Science, University of Tokyo. This work was supported by Grants-in-Aid for Research Fellowships for Young Scientists (No.20-9894 to SM) from the Japan Society for the Promotion of Science; Creative Scientific Research (No. 16GS0304 to HN) and Scientific Research (No. 20247032 to HN) from The Ministry of Education, Culture, Sports, Science, and Technology, Japan.
- Timmis JN, Ayliffe MA, Huang CY, Martin W: Endosymbiotic gene transfer: organelle genomes forge eukaryotic chromosomes. Nat Rev Genet. 2004, 5: 123-135. 10.1038/nrg1271.View ArticlePubMedGoogle Scholar
- Delwiche CF: Tracing the thread of plastid diversity through the tapestry of life. Am Nat. 1999, 154: 164-177. 10.1086/303291.View ArticleGoogle Scholar
- Bhattacharya D, Yoon HS, Hackett JD: Photosynthetic eukaryotes unite: endosymbiosis connects the dots. Bioessays. 2004, 26: 50-60. 10.1002/bies.10376.View ArticlePubMedGoogle Scholar
- Baldauf SL: The deep roots of eukaryotes. Science. 2003, 300: 1703-1706. 10.1126/science.1085544.View ArticlePubMedGoogle Scholar
- Stechmann A, Cavalier-Smith T: Rooting the eukaryote tree by using a derived gene fusion. Science. 2002, 297: 89-91. 10.1126/science.1071196.View ArticlePubMedGoogle Scholar
- Stechmann A, Cavalier-Smith T: The root of the eukaryote tree pinpointed. Curr Biol. 2003, 13: 665-666. 10.1016/S0960-9822(03)00602-X.View ArticleGoogle Scholar
- Nozaki H, Matsuzaki M, Misumi O, Kuroiwa H, Higashiyama T, Kuroiwa T: Phylogenetic implications of the CAD complex from the primitive red alga Cyanidioschyzon merolae (Cyanidiales, Rhodophyta). J Phycol. 2005, 41: 652-657. 10.1111/j.1529-8817.2005.00079.x.View ArticleGoogle Scholar
- Richards TA, Cavalier-Smith T: Myosin domain evolution and the primary divergence of eukaryotes. Nature. 2005, 436: 1113-1118. 10.1038/nature03949.View ArticlePubMedGoogle Scholar
- Roger AJ, Simpson AGB: Revisiting the root of the eukaryote tree. Curr Biol. 2009, 19: 165-167. 10.1016/j.cub.2008.12.032.View ArticleGoogle Scholar
- Adl SM, Simpson AG, Farmer MA, Andersen RA, Anderson OR, Barta JR, Bowser SS, Brugerolle G, Fensome RA, Fredericq S, James TY, Karpov S, Kugrens P, Krug J, Lane CE, Lewis LA, Lodge J, Lynn DH, Mann DG, McCourt RM, Mendoza L, Moestrup O, Mozley-Standridge SE, Nerad TA, Shearer CA, Smirnov AV, Spiegel FW, Taylor MF: The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J Eukaryot Microbiol. 2005, 52: 399-451. 10.1111/j.1550-7408.2005.00053.x.View ArticlePubMedGoogle Scholar
- Matsuzaki M, Misumi O, Shin-I T, Maruyama S, Takahara M, Miyagishima SY, Mori T, Nishida K, Yagisawa F, Nishida K, Yoshida Y, Nishimura Y, Nakao S, Kobayashi T, Momoyama Y, Higashiyama T, Minoda A, Sano M, Nomoto H, Oishi K, Hayashi H, Ohta F, Nishizaka S, Haga S, Miura S, Morishita T, Kabeya Y, Terasawa K, Suzuki Y, Ishii Y, et al: Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature. 2004, 428: 653-657. 10.1038/nature02398.View ArticlePubMedGoogle Scholar
- Reyes-Prieto A, Bhattacharya D: Phylogeny of calvin cycle enzymes supports plantae monophyly. Mol Phylogenet Evol. 2007, 45: 384-391. 10.1016/j.ympev.2007.02.026.View ArticlePubMedGoogle Scholar
- Tyra HM, Linka M, Weber AP, Bhattacharya D: Host origin of plastid solute transporters in the first photosynthetic eukaryotes. Genome Biol. 2007, 8: R212-10.1186/gb-2007-8-10-r212.PubMed CentralView ArticlePubMedGoogle Scholar
- Larkum AW, Lockhart PJ, Howe CJ: Shopping for plastids. Trends Plant Sci. 2007, 12: 189-195. 10.1016/j.tplants.2007.03.011.View ArticlePubMedGoogle Scholar
- Stiller JW: Plastid endosymbiosis, genome evolution and the origin of green plants. Trends Plant Sci. 2007, 12: 391-396. 10.1016/j.tplants.2007.08.002.View ArticlePubMedGoogle Scholar
- Rodríguez-Ezpeleta N, Brinkmann H, Burey SC, Roure B, Burger G, Löffelhardt W, Bohnert HJ, Philippe H, Lang BF: Monophyly of primary photosynthetic eukaryotes: green plants, red algae, and glaucophytes. Curr Biol. 2005, 15: 1325-1330. 10.1016/j.cub.2005.06.040.View ArticlePubMedGoogle Scholar
- Nozaki H, Iseki M, Hasegawa M, Misawa K, Nakada T, Sasaki N, Watanabe M: Phylogeny of primary photosynthetic eukaryotes as deduced from slowly evolving nuclear genes. Mol Biol Evol. 2007, 24: 1592-1595. 10.1093/molbev/msm091.View ArticlePubMedGoogle Scholar
- Hampl V, Hug L, Leigh JW, Dacks JB, Lang BF, Simpson AGB, Roger AJ: Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic "supergroups". Proc Nat Acad Sci USA. 2009, 106: 3859-3864. 10.1073/pnas.0807880106.PubMed CentralView ArticlePubMedGoogle Scholar
- Nozaki H: A new scenario of plastid evolution: plastid primary endosymbiosis before the divergence of the "Plantae," emended. J Plant Res. 2005, 118: 247-255. 10.1007/s10265-005-0219-1.View ArticlePubMedGoogle Scholar
- Andersson JO, Roger AJ: A cyanobacterial gene in nonphotosynthetic protists – an early chloroplast acquisition in eukaryotes?. Curr Biol. 2002, 12: 115-119. 10.1016/S0960-9822(01)00649-2.View ArticlePubMedGoogle Scholar
- Maruyama S, Misawa K, Iseki M, Watanabe M, Nozaki H: Origins of a cyanobacterial 6-phosphogluconate dehydrogenase in plastid-lacking eukaryotes. BMC Evol Biol. 2008, 8: 151-10.1186/1471-2148-8-151.PubMed CentralView ArticlePubMedGoogle Scholar
- Sanchez-Puerta MV, Delwiche CF: A hypothesis for plastid evolution in chromalveolates. J Phycol. 2008, 44: 1097-1107. 10.1111/j.1529-8817.2008.00559.x.View ArticleGoogle Scholar
- Cavalier-Smith T: Principles of protein and lipid targeting in secondary symbiogenesis: euglenoid, dinoflagellate, and sporozoan plastid origins and the eukaryote family tree. J Eukaryot Microbiol. 1999, 46: 347-366. 10.1111/j.1550-7408.1999.tb04614.x.View ArticlePubMedGoogle Scholar
- Foth BJ, McFadden GI: The apicoplast: a plastid in Plasmodium falciparum and other apicomplexan parasites. Int Rev Cytol. 2003, 224: 57-110. 10.1016/S0074-7696(05)24003-2.View ArticlePubMedGoogle Scholar
- Matsuzaki M, Kuroiwa H, Kuroiwa T, Kita K, Nozaki H: A cryptic algal group unveiled: a plastid biosynthesis pathway in the oyster parasite Perkinsus marinus. Mol Biol Evol. 2008, 25: 1167-1179. 10.1093/molbev/msn064.View ArticlePubMedGoogle Scholar
- Slamovits CH, Keeling PJ: Plastid-derived genes in the non-photosynthetic alveolate Oxyrrhis marina. Mol Biol Evol. 2008, 25: 1297-1306. 10.1093/molbev/msn075.View ArticlePubMedGoogle Scholar
- Reyes-Prieto A, Moustafa A, Bhattacharya D: Multiple genes of apparent algal origin suggest ciliates may once have been photosynthetic. Curr Biol. 2008, 18: 956-962. 10.1016/j.cub.2008.05.042.PubMed CentralView ArticlePubMedGoogle Scholar
- Tyler BM, Tripathy S, Zhang X, Dehal P, Jiang RH, Aerts A, Arredondo FD, Baxter L, Bensasson D, Beynon JL, Chapman J, Damasceno CM, Dorrance AE, Dou D, Dickerman AW, Dubchak IL, Garbelotto M, Gijzen M, Gordon SG, Govers F, Grunwald NJ, Huang W, Ivors KL, Jones RW, Kamoun S, Krampis K, Lamour KH, Lee MK, McDonald WH, Medina M, et al: Phytophthora genome sequences uncover evolutionary origins and mechanisms of pathogenesis. Science. 2006, 313: 1261-1266. 10.1126/science.1128796.View ArticlePubMedGoogle Scholar
- Koski LB, Golding GB: The closest BLAST hit is often not the nearest neighbor. J Mol Evol. 2001, 52: 540-542.View ArticlePubMedGoogle Scholar
- Nozaki H, Matsuzaki M, Misumi O, Kuroiwa H, Hasegawa M, Higashiyama T, Shin-I T, Kohara Y, Ogasawara N, Kuroiwa T: Cyanobacterial genes transmitted to the nucleus before divergence of red algae in the Chromista. J Mol Evol. 2004, 59: 103-113. 10.1007/s00239-003-2611-1.View ArticlePubMedGoogle Scholar
- Schuster FL, Visvesvara GS: Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol. 2004, 34: 1001-1027. 10.1016/j.ijpara.2004.06.004.View ArticlePubMedGoogle Scholar
- Simpson AG, Perley TA, Lara E: Lateral transfer of the gene for a widely used marker, alpha-tubulin, indicated by a multi-protein study of the phylogenetic position of Andalucia (Excavata). Mol Phylogenet Evol. 2008, 47: 366-377. 10.1016/j.ympev.2007.11.035.View ArticlePubMedGoogle Scholar
- Archibald JM, Rogers MB, Toop M, Ishida K, Keeling PJ: Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga Bigelowiella natans. Proc Natl Acad Sci USA. 2003, 100: 7678-7683. 10.1073/pnas.1230951100.PubMed CentralView ArticlePubMedGoogle Scholar
- Gray J, Wardzala E, Yang M, Reinbothe S, Haller S, Pauli F: A small family of LLS1-related non-heme oxygenases in plants with an origin amongst oxygenic photosynthesizers. Plant Mol Biol. 2004, 54: 39-54. 10.1023/B:PLAN.0000028766.61559.4c.View ArticlePubMedGoogle Scholar
- Gross J, Bhattacharya D: Revaluating the evolution of the Toc and Tic protein translocons. Trends Plant Sci. 2009, 14: 13-20. 10.1016/j.tplants.2008.10.003.View ArticlePubMedGoogle Scholar
- Huang J, Mullapudi N, Lancto CA, Scott M, Abrahamsen MS, Kissinger JC: Phylogenomic evidence supports past endosymbiosis, intracellular and horizontal gene transfer in Cryptosporidium parvum. Genome Biol. 2004, 5: R88-10.1186/gb-2004-5-11-r88.PubMed CentralView ArticlePubMedGoogle Scholar
- Moreira D, Heyden von der S, Bass D, López-García P, Chao E, Cavalier-Smith T: Global eukaryote phylogeny: combined small- and large-subunit ribosomal DNA trees support monophyly of Rhizaria, Retaria and Excavata. Mol Phylogenet Evol. 2007, 44: 255-266. 10.1016/j.ympev.2006.11.001.View ArticlePubMedGoogle Scholar
- Hackett JD, Yoon HS, Li S, Reyes-Prieto A, Rümmele SE, Bhattacharya D: Phylogenomic analysis supports the monophyly of cryptophytes and haptophytes and the association of rhizaria with chromalveolates. Mol Biol Evol. 2007, 24: 1702-1713. 10.1093/molbev/msm089.View ArticlePubMedGoogle Scholar
- Patron NJ, Inagaki Y, Keeling PJ: Multiple gene phylogenies support the monophyly of cryptomonad and haptophyte host lineages. Curr Biol. 2007, 17: 887-891. 10.1016/j.cub.2007.03.069.View ArticlePubMedGoogle Scholar
- Burki F, Shalchian-Tabrizi K, Pawlowski J: Phylogenomics reveals a new 'megagroup' including most photosynthetic eukaryotes. Biol Lett. 2008, 4: 366-369. 10.1098/rsbl.2008.0224.PubMed CentralView ArticlePubMedGoogle Scholar
- Minge M, Silberman JD, Orr R, Cavalier-Smith T, Shalchian-Tabrizi K, Burki F, Skjæveland Å, Jakobsen KS: Evolutionary position of breviate amoebae and the primary eukaryote divergence. Proc Biol Sci. 2009, 276: 597-604. 10.1098/rspb.2008.1358.View ArticlePubMedGoogle Scholar
- Frommolt R, Werner S, Paulsen H, Goss R, Wilhelm C, Zauner S, Maier UG, Grossman AR, Bhattacharya D, Lohr M: Ancient recruitment by chromists of green algal genes encoding enzymes for carotenoid biosynthesis. Mol Biol Evol. 2008, 25: 2653-2667. 10.1093/molbev/msn206.View ArticlePubMedGoogle Scholar
- Leander BS: Did trypanosomatid parasites have photosynthetic ancestors?. Trends Microbiol. 2004, 12: 251-258. 10.1016/j.tim.2004.04.001.View ArticlePubMedGoogle Scholar
- Keeling PJ, Inagaki Y: A class of eukaryotic GTPase with a punctate distribution suggesting multiple functional replacements of translation elongation factor 1alpha. Proc Natl Acad Sci USA. 2004, 101: 15380-15385. 10.1073/pnas.0404505101.PubMed CentralView ArticlePubMedGoogle Scholar
- Rogers MB, Watkins RF, Harper JT, Durnford DG, Gray MW, Keeling PJ: A complex and punctate distribution of three eukaryotic genes derived by lateral gene transfer. BMC Evol Biol. 2007, 7: 89-10.1186/1471-2148-7-89.PubMed CentralView ArticlePubMedGoogle Scholar
- Shalchian-Tabrizi K, Minge MA, Espelund M, Orr R, Ruden T, Jakobsen KS, Cavalier-Smith T: Multigene phylogeny of choanozoa and the origin of animals. PLoS ONE. 2008, 3: e2098-10.1371/journal.pone.0002098.PubMed CentralView ArticlePubMedGoogle Scholar
- Boldt R, Edner C, Kolukisaoglu U, Hagemann M, Weckwerth W, Wienkoop S, Morgenthal K, Bauwe H: D-GLYCERATE 3-KINASE, the last unknown enzyme in the photorespiratory cycle in Arabidopsis, belongs to a novel kinase family. Plant Cell. 2005, 17: 2413-2420. 10.1105/tpc.105.033993.PubMed CentralView ArticlePubMedGoogle Scholar
- Eisenhut M, Ruth W, Haimovich M, Bauwe H, Kaplan A, Hagemann M: The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants. Proc Natl Acad Sci USA. 2008, 105: 17199-17204. 10.1073/pnas.0807043105.PubMed CentralView ArticlePubMedGoogle Scholar
- Slot JC, Hallstrom KN, Matheny PB, Hibbett DS: Diversification of NRT2 and the origin of its fungal homolog. Mol Biol Evol. 2007, 24: 1731-43. 10.1093/molbev/msm098.View ArticlePubMedGoogle Scholar
- Wilhelm C, Büchel C, Fisahn J, Goss R, Jakob T, Laroche J, Lavaud J, Lohr M, Riebesell U, Stehfest K, Valentin K, Kroth PG: The regulation of carbon and nutrient assimilation in diatoms is significantly different from green algae. Protist. 2006, 157: 91-124. 10.1016/j.protis.2006.02.003.View ArticlePubMedGoogle Scholar
- Roberts K, Granum E, Leegood RC, Raven JA: C3 and C4 pathways of photosynthetic carbon assimilation in marine diatoms are under genetic, not environmental, control. Plant Physiol. 2007, 145: 230-235. 10.1104/pp.107.102616.PubMed CentralView ArticlePubMedGoogle Scholar
- Eldan MB, Blum J: Presence of Nonoxidative Enzymes of the Pentose Phosphate Shunt in Tetrahymena. J Eurkaryot Microbiol. 1975, 22: 145-149. 10.1111/j.1550-7408.1975.tb00962.x.Google Scholar
- de la Torre F, De Santis L, Suárez MF, Crespillo R, Cánovas FM: Identification and functional analysis of a prokaryotic-type aspartate aminotransferase: implications for plant amino acid metabolism. Plant J. 2006, 46: 414-425. 10.1111/j.1365-313X.2006.02713.x.View ArticlePubMedGoogle Scholar
- DOE Joint Genome Institute. [http://www.jgi.doe.gov/]
- DictyBase: An Online Informatics Resource for Dictyostelium. [http://dictybase.org/]
- MIPS Comprehensive Yeast Genome Database. [http://mips.gsf.de/genre/proj/yeast/]
- Cyanidioschyzon merolae Genome Database. [http://merolae.biol.s.u-tokyo.ac.jp/]
- Galdieria sulphuraria Genome Database. [http://genomics.msu.edu/galdieria/]
- Taxonomically broad EST database TBestDB. [http://tbestdb.bcm.umontreal.ca/]
- National Center for Biotechnology Information (NCBI). [http://www.ncbi.nlm.nih.gov/]
- Stiller JW, Duffield EC, Hall BD: Amitochondriate amoebae and the evolution of DNA-dependent RNA polymerase II. Proc Natl Acad Sci USA. 1998, 95: 11769-11774. 10.1073/pnas.95.20.11769.PubMed CentralView ArticlePubMedGoogle Scholar
- Stiller JW, Riley J, Hall BD: Are red algae plants? A critical evaluation of three key molecular data sets. J Mol Evol. 2001, 52: 527-539.View ArticlePubMedGoogle Scholar
- Eisen JA, Coyne RS, Wu M, Wu D, Thiagarajan M, Wortman JR, Badger JH, Ren Q, Amedeo P, Jones KM, Tallon LJ, Delcher AL, Salzberg SL, Silva JC, Haas BJ, Majoros WH, Farzad M, Carlton JM, Smith RK, Garg J, Pearlman RE, Karrer KM, Sun L, Manning G, Elde NC, Turkewitz AP, Asai DJ, Wilkes DE, Wang Y, Cai H, et al: Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. PLoS Biol. 2006, 4: e286-10.1371/journal.pbio.0040286.PubMed CentralView ArticlePubMedGoogle Scholar
- NCBI netblast. [http://www.ncbi.nlm.nih.gov/BLAST/download.shtml]
- NCBI EFetch utilities. [http://eutils.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?]
- Edgar RC: MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, 32: 1792-1797. 10.1093/nar/gkh340.PubMed CentralView ArticlePubMedGoogle Scholar
- Howe K, Bateman A, Durbin R: QuickTree: building huge Neighbour-Joining trees of protein sequences. Bioinformatics. 2002, 18: 1546-1547. 10.1093/bioinformatics/18.11.1546.View ArticlePubMedGoogle Scholar
- Perrière G, Gouy M: WWW-query: an on-line retrieval system for biological sequence banks. Biochimie. 1996, 78: 364-369. 10.1016/0300-9084(96)84768-7.View ArticlePubMedGoogle Scholar
- NCBI taxonomy database. [http://www.ncbi.nlm.nih.gov/Taxonomy]
- Stamatakis A, Hoover P, Rougemont J: A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol. 2008, 57: 758-771. 10.1080/10635150802429642.View ArticlePubMedGoogle Scholar
- Huelsenbeck JP, Ronquist F: MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001, 17: 754-755. 10.1093/bioinformatics/17.8.754.View ArticlePubMedGoogle Scholar