Maximum parsimonious (50% majority-rule consensus) trees of the ncp GS gene. a. For the diploid species Achillea setacea and A. asplenifolia only based on 13 consensus sequences and two equally most parsimonious trees (tree length = 119, CI = 0.8824, RI = 0.9343). b. For all the studied diploid and tetraploid species and populations based on 80 consensus sequences and 8700 equally most parsimonious trees (tree length = 403, CI = 0.4491, RI = 0.8649). Bootstrap supports (>50%) from MP/NJ analyse are shown above/below the major branches. Label for the sequence (terminal node) is written as "taxa abbreviation # population code (number of individuals/number of clones)". Abbreviations: asp = A. asplenifolia-2x, set = A. setacea-2x, col = A. collina-4x, mixA = mixed populations of A. asplenifolia-2x and suspected A. asplenifolia x collina-4x backcross individuals, mixS = mixed populations of A. setacea-2x and suspected A. setacea × collina-4x backcross individuals.