Different GIF1 and OsCIN1 expression patterns. (A) Levels of the GIF1 and OsCIN1 transcripts were detected by RT-PCR in different tissues. (B) Levels of the GIF1 and OsCIN1 transcripts during grain filling. Note that OsCIN1 was constitutively expressed in developing grains. The experiments were repeated twice with similar results, Ubi-1 was used as a loading control for RT-PCR. DAP, day after pollination; Cyc, PCR cycles.