Figure 3

Defining the kinesin repertoire of the last common eukaryotic ancestor (LCEA). We considered 5 rooted eukaryotic trees to infer conservative estimates of the minimal ancestral repertoire of kinesins present in the LCEA using Dollo parsimony: A) 'Metamonada-first'; B) 'Discicristata-first'; C) 'Excavata-first'; D) root between unikonts and bikonts; E) Dollo most parsimonious tree necessary to explain the extant distribution (boxed in red). The unconstrained most parsimonious tree gives an unrealistic eukaryotic tree topology and therefore is likely to underestimate the LCEA repertoire (see Results and Discussion). The parsimony scores under the two alternative datasets are shown for all 5 topologies. Also shown are the results of SH alternative topology tests for the four alternative models under the polytomy favored by the analysis of Burki et al. [26] (see Results and Discussion). Paralog families and kinesin architectures, which must have been present in the LCEA given the tree topology are shown beneath each tree. Kinesin paralogs are colored blue for families (K1-20) and green for non-families (X1-14; see Figure 1). Kinesin protein domain architectures are shown in black (see Figure 2). These analyses indicate minimally 18 to 29 kinesin characters (paralogs/architectures) in the LCEA. Kinesin characters present in the LCEA under the 4 leading models of the eukaryotic tree topology, A-D (the minimal ancestral repertoire - MAR) are marked in bold.