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Figure 5 | BMC Evolutionary Biology

Figure 5

From: Tubulin evolution in insects: gene duplication and subfunctionalization provide specialized isoforms in a functionally constrained gene family

Figure 5

β2 mRNA expression in Drosophila testis tissue. Testis expression of Beta 2 message was tested in D. ananassae, D. willistoni, D. persimilis, and D. psuedoobscura, species in which the B2UE1 testis cis-regulatory sequence was not identifiable (Table 6). All were found to express Beta 2 in the testis, indicating compensatory mutation in testis cis-regulation has occurred to maintain their testis expression. Lane Numbers (L to R) 1. Ladder (bottom most band = 500 bp). 2. blank 3. D. willistoni RT-PCR 4. D. willistoni Taq PCR 5. D. ananassae RT-PCR 6. D. ananassae Taq PCR 7. D. persimilis RT-PCR 8. D. persimilis Taq PCR 9. D. pseudoobscura RT-PCR 10. D. pseudoobscura Taq PCR.

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