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Table 6 Drosophila β2 cis-regulatory sequences.

From: Tubulin evolution in insects: gene duplication and subfunctionalization provide specialized isoforms in a functionally constrained gene family

Species

B2UE1

B2UE2

Inr

B2DE1

ATG

D. melanogaster

(-51)

ATCGTAGTAGCCTA

(-32)

GAACAT

(-3)

TTCAGTT

(+51)

AAAATTATACGTTTAAAT

+172

D. ananassae

(-103)

ACCCGAGTATCGTT

(-57)

GAACAG

(-3)

TCCACCT

(+47)

AAAATTGTACGTTAAAAA

+212

D. erecta

(-51)

ATCGTAGTAGCCCA

(-32)

GAACAT

(-3)

TTCAGTC

(+51)

AAAATTATACGTTTAAAT

+211

D. grimshawi

(-328)

ATCAGAATTGTTCG

(-256)

GAATAT

(-3)

CTCATTC

(+49)

AAAATTAAACGTGAAAAA

+155

D. mojavensis

(-51)

ATCCCAGTAGTTCC

(-32)

GTACAT

(-3)

CTCATTC

(+48)

AAAATTATACGTTAAAAT

+187

D. persimilis

(-304)

CATGTAGAGACCCA

(-55)

GAACAA

(-3)

CTCATTC

(+43)

TAACTTAAAAAATTCATT

+196

D. pseudoobscura

(-304)

CATGTAGAGACCCA

(-55)

GAACAA

(-3)

CTCATTC

(+43)

TAACTTAAAAAATTCATT

+195

D. sechellia

(-51)

ATCGCAGTAGCCTA

(-32)

GAACAT

(-3)

TTCAGTT

(+51)

AAAATTATACGTTTAAAT

+169

D. simulans

(-51)

ATCGCAGTAGCCTA

(-32)

GAACAT

(-3)

TTCAGTT

(+51)

AAAATTATACGTTTAAAT

+165

D. virilis

(-51)

ATCGAAGTAGTCTA

(-32)

GGACAT

(-3)

CTCATTC

(+48)

AAAATTATACGTAAAAAT

+169

D. willistoni

(-189)

ATCGAAGAATATTA

(-165)

GAACAT

-(3)

TCCAGCT

(+46)

AAAATTATTCGTACAAAA

+205

D. yakuba

(-51)

ATCGTAGTAGCCCA

(-32)

GAATAT

(-3)

CTCAGTC

(+51)

AAAATTATACGTTTAAAT

+155

Anopheles gambiae

(-79)

GCCGTACGTGCCGG

(-52)

GAACCT

(-3)

TCCATTC

(+45)

AAACTAGAAATTTGTGTA

+188

  1. The cis-regulatory elements required for β2 tubulin expression in the testis at appropriate levels have been identified in D. melanogaster; these sequences (when identifiable) are presented for other Drosophila species. Numerical positions indicating sequence position relative to transcription start site (= +1). The B2UE1 element is required for testis-specific gene expression, the B2UE2 and B2DE1 elements for proper expression levels, the Inr element is part of the β2 core promotor, and ATG is the start of β2 coding sequence.