Figure 7

Internal-primer SINE PCR was conducted to examine the species distribution of HAmo SINE. The PCR was run in a total volume of 25 μl with 25 cycles of 95°C for 40s, 58°C for 40s, and 72°C for 40s. Four ten-fold DNA: 0.1ng, 1ng, 10ng, 100ng were used as template for each species. The expected fragment was about 130 bp. A) The investigated species from Cyprinidae in this paper. From number 1-17: 1. Hypophthalmichthys molitrix; 2. Aristichthys nobilis; 3. Distoechodon hupeinensis; 4. Xenocypris argentea; 5. Cutler alburnus; 6. Leuciscus tumensis; 7. Ctenopharyngodon idellus; 8. Mylopharyngodon piceus; 9. Squaliobarbus curriculus; 10. Elopichthys bambusa; 11. Rhodeus ocellatus; 12. Saurogobio dabryi; 13. Cyprinus carpio; 14. Shizothorax grahami; 15. Barbodes opisthoptera; 16. Opsariichthys bidens; 17. Garra orientalis. B) Four species were used for representing four families of order Cypriniforms: Myxocyprinus asiaticus (Catostomidae), Misgurnus anguillicaudatus (Cobitidae), Gyrinocheilus aymonieri (Gyrinocheilidae) and Hemimyzon abbreviata (Homalopteridae). C) In Siluriformes, two species were used: Gagata dolichonema (Sisoridae), Pseudobagrus truncatus (Bagridae). Other orders:18. Polypterus delhezi (Polypteriformes); 19. Lepisosteus osseus (Lepidosteiformes); 20. Atractosteus tropicus (Lepidosteiformes); 21. Atractosteus spatula (Lepidosteiformes); 22. Lepisosteus platyrhincus (Lepidosteiformes); 23. Lepisosteus platostomus (Lepidosteiformes). 24. Polyodon spathala (Acipenseriformes); 25. Osteoglossiforms; 26. Acipenser sinensis (Acipenseriformes).