Table 2 Copy number estimation of SINE by quantitative RT-PCR

Plasmid (Hmo_41)

0.2 μg/μl

11.10

Y = -0.2989x+14.22

0.02 μg/μl

16.30

Y = -0.2989x+14.22

0.002 μg/μl

18.36

R ^2 = 0.997; E = 0.99

0.0002 μg/μl

21.72

Results:

plasimid DNA size = 2× 103 bp

copy number of plasmid per μl

= 6.5× 10¹¹ (2 ug/ul)

0.00002 μg/μl

25.30

Sample

Con/(ug/ul)

CT

PCNH

Avg.

Xenocypris argentea

0.115

13.32

183221.1127

3.0 × 10⁵

0.0115

15.44

425899.8786

Cutler alburnus

0.9

12.12

53470.08164

3.5× 10⁴

0.09

17.11

17242.48462

Elopichthys bambusa

0.133

15.32

39996.47484

4.0 × 10⁴

0.0133

18.69

39330.43191

Squaliobarbus curriculus

(Genome size≈ 1 pg)

0.1

13.83

148331.8306

1.7 × 10⁵

0.01

16.8

192088.4962

Mylopharyngodon piceus

(Genome size≈ 1 pg)

0.36

15.04

17916.89943

1.7× 10⁴

0.036

18.46

17022.5593

Ctenopharyngodon idellus

(Genome size≈ 1 pg)

0.314

15.32

16941.18202

1.7 × 10⁴

0.0314

18.69

16659.06829

Leuciscus tumensis

0.104

13.32

202600.2689

3.3× 10⁵

0.0104

15.44

470946.9811

Saurogobio dabryi

0.03

11.91

1853536.326

1.7× 10⁶

0.003

15.51

1555826.276

Rhodeus ocellatus

(Genome size≈ 1 pg)

0.092

13.91

152592.9712

2.2× 10⁵

0.0092

16.23

309091.1208

Cyprinus carpio

(Genome size≈ 2 pg)

0.0033

22.06

31174.78108

3.1× 10⁴

0.00033

25.39

31511.30668

Shizothorax grahami

0.08

10.66

3286149.864

5.1× 10⁶

0.008

12.87

7179899.5