Figure 3

Mapping of the WD40 repeat deletions. A/On this schematic representation of the het-R WD repeat domain dashed lines delineate the WD40 repeat sequences and BglII restriction sites are labelled B1 to B6. At the top are indicated the size of the expected BglII generated restriction fragments and below are indicated the size of the non repeat fragments from the end of the amplicon to the beginning of the repeated sequences. B/For the three most represented subclasses of mutants, the intervals in which deletion occur are represented. Top labels refer to the intervals defined (additional file 2). Numbers below each interval represent the number of observed vs the number of expected mutants is generated at random. C/Representative agarose gel presenting the BglII restriction profiles of the wild type het-R allele along with selected mutants. F4, N5 and E4 belong to the -1 subclass, L16 to the -2 subclass and M14 to the -3 subclass.