Analyses of the splicing pattern of tra (A) and dsx (B) pre-mRNAs of A. sp.1 aff. fraterculus in XX pseudomales developed from eggs injected with Aotra2 dsRNA. F and M indicates normal female and male respectively. The sequences of the TraAo41 and TraAo44 primers used, the locations of which are shown with arrows in Figure 4A, are described in Ruiz et al. ; the sequences of the dsxA26, dsxAo32F and dsxAo35M primers used, the locations of which is shown with arrows in Figure 4B, are described in Ruiz et al. .