Transcript and protein profiling identify candidate gene sets of potential adaptive significance in New Zealand Pachycladon

Table 5 Glucosinolate hydrolysis products (nmol/g fresh weight, mean ± SD) of P. novae-zelandiae and P. cheesemanii

Note that P. novae-zelandiae is capable of converting its main glucosinoalates (S-2-hydroxy-3-butenyl and allyl) into its corresponding nitriles ((S)-3-hydroxy-pent-4-enenitril) and epithionitriles (diastereomers 1 and 2 of (S)-3-hydroxy-3-((R, S)-thiiran-2-yl)propanenitril, 2-((R, S)-thiiran-2-yl)ethanenitril) whereas P. cheesemanii is not. Despite minor glucosinolates being converted to isothiocyanates in P. novae-zelandiae, 65% of its glucosinolate breakdown products are nitriles/epithionitriles. In P. cheesemanii, 99.5% of glucosinolates are converted to isothiocyanates indicating nitrile/epithionitrile forming activity in P. novae-zelandiae but not P. cheesemanii.
Abbreviations: goitrin, goitrin; allyl-ITC, 2-propenylisothiocyanate; 3-but-ITC, 3-butenylisothiocyanate; 3MTP-ITC, 3-methylthiopropylisothiocyanate; 3MSOP-ITC, 3-methylsulfinylpropylisothiocyanate; 4MTB-ITC, 4-methylthiopropylisothiocynate; 4MSOB-ITC, 4-methylsulfinylbutylisothiocyanate; 8MTO-ITC, 8-methylthiooctylisothiocyanate; 9MTN-ITC, 9-methylthiononyl-isothiocyanate; 2OH3but-CN, S-3-hydroxy-pent-4-enenitrile; epithio-2OH3B-I, diastereomer 1 of (S)-3-hydroxy-3-((R, S)-thiiran-2-yl)propanenitril; epithio-2OH3B-II, diastereomer 2 of (S)-3-hydroxy-3-((R, S)-thiiran-2-yl)propanenitril; epithio-allyl, 2-((R, S)-thiiran-2-yl)ethanenitril; 3MTP-CN, 4-methylthiobutanenitrile; 4MTB-CN, 5-methylthiopentanenitrile.

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