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Table 1 Kinetic characteristics of GST-bfAANAT and GST-bfAANAT ' for selected amines.

From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

 

Substrate

 

Phenylethylamine

Tyramine

Serotonin

Tryptamine

bfAANAT

α

δ'

α

δ'

α

δ'

α

δ'

V max

17.8 ± 1.1

4.9 ± 3

2.06 ± 0.28

0.78 ± .06

1.60 ± 0.1

0.56 ± 0.06

4.7 ± 0.8

2.7 ± 0.25

K m

3 ± 0.6

45 ± 36

8.2 ± 2.70

0.80 ± 0.28

0.01 ± 0.01

<0.01

8.1 ± 3.6

2.5 ± 0.7

 

Substrate

 

Octopamine

Propylamine

Butylamine

Putrescine

bfAANAT

α

δ'

α

δ'

α

δ'

α

δ'

V max

2.1 ± 0.2

0.80 ± 0.05

>10

0.73 ± 0.07

32.5 ± 4.1

0.7 ± 0.05

1.34 ± 0.32

5.11 ± 0.11

K m

6.8 ± 1.9

0.05 ± 0.01

>20

0.90 ± 0.37

47.5 ± 8.0

0.37 ± 0.23

5.2 ± 4.0

3.68 ± 0.22

  1. Activity was measured at pH 9.0. Values are calculated from data in Figure 7. V max values are nmol/h/pmol enzyme and are given as the mean ± S.E.M. (N = 3 to 6). For further details see Methods.