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Table 3 List of the primers used in this study

From: Unsuspected diversity of Niphargus amphipods in the chemoautotrophic cave ecosystem of Frasassi, central Italy

Marker

Direction

Purpose

Sequence

Reference

COI

Forward

PCR + sequencing

5'-GGTCAACAAATCATAAAGATATTGG-3'

[66]

COI

Reverse

PCR + sequencing

5'-TAAACTTCAGGGTGACCAAAAAATCA-3'

[66]

12S

Forward

PCR + sequencing

5'-GCCAGCAGCCGCGGTTA-3'

[67]

12S

Reverse

PCR + sequencing

5'-CCTACTTTGTTACGACTTAT-3'

[67]

28S

Forward

PCR + sequencing

5'-CAAGTACCGTGAGGGAAAGTT-3'

[68]

28S

Reverse

PCR + sequencing

5'-AGGGAAACTTCGGAGGGAACC-3'

[68]

28S

Forward

sequencing

5'-AAACACGGGCCAAGGAGTAT-3'

this article

28S

Reverse

sequencing

5'-TATACTCCTTGGCCCGTGTT-3'

this article

ITS

Forward

PCR + sequencing

5'-TCCGAACTGGTGCACTTAGA-3'

this article

ITS

Reverse

PCR + sequencing

5'-TCCAAGCTCCATTGGCTTAT-3'

this article

ITS

Forward

sequencing

5'-CGCTGCCATTCTCACACTTA-3'

this article

ITS

Reverse

sequencing

5'-ACTCTGAGCGGTGGATCACT-3'

this article

ITS

Forward

sequencing

5'-AAGGCTATAGCTGGCGATCA-3'

this article

ITS

Reverse

sequencing

5'-TCAGCGGGTAACCTCTCCTA-3'

this article