Transcript ID/Query | CDS Length | 0-2 hr | 2-4 hr | 4-8 hr | 8-12 hr |
---|
AAEL011410-RA | 1419 | 0 (0) | 250 (366) | 405 (728) | 0 (0) |
AAEL014967-RA | 1419 | 0 (0) | 240 (352) | 436 (784) | 1 (4) |
AAEL012472-RA | 1536 | 62 (193) | 1103 (1493) | 311 (516) | 140 (516) |
- Notes: 0-2, 2-4, 4-8, and 8-12 hr indicate time ranges from which RNA was isolated from developing Ae. aegypti embryos. Raw values of the three transcripts in the four samples (number of hits from BLAST using an E-value cutoff of 1e-7) are given at left. Normalized values (in parentheses) are calculated as the raw values divided by the transcript (CDS) length, then divided by the total number of reads matching all annotated mRNA transcripts from each sample, and multiplied by 1e10. The total number of reads matching all annotated mRNA transcripts from each sample time point was: 0-2 hr (2087707); 2-4 hr (4810435); 4-8 hr (3920821); 8-12 hr (1767898). See Additional File 1 for a summary of the Illumina data. A FASTA file of all Illumina sequence hits that match the three kinesin light chain transcripts in all four samples is provided as Additional File 2.