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Figure 6 | BMC Evolutionary Biology

Figure 6

From: Interference with histidyl-tRNA synthetase by a CRISPR spacer sequence as a factor in the evolution of Pelobacter carbinolicus

Figure 6

Growth of G. sulfurreducens with hisS of P. carbinolicus is inhibited by spacer #1. (a) Growth on NBAF medium by reduction of fumarate. (b) Growth on FWAFC medium by reduction of Fe(III) citrate. The strains shown are wild type G. sulfurreducens DL1 (black squares); DL1(pMA35-2) with two copies of spacer #1 in a plasmid-borne chimeric CRISPR (white squares); transgenic strain MA159, which has hisS of P. carbinolicus (black diamonds); MA159(pMA35-2) with both the hisS transgene and two copies of spacer #1 (white diamonds); and MA159(pMA35-0) with the hisS transgene and a CRISPR repeat without spacer #1 (grey diamonds).

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