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Figure 5 | BMC Evolutionary Biology

Figure 5

From: Lineage-specific co-evolution of the Egf receptor/ligand signaling system

Figure 5

Western blot analysis of medaka Egfra activation by human EGF and by medaka Egfr ligands. A) Melan-a WT, melan-a Ola-egfra and melan-a HER cells were stimulated for 30 minutes with 100 ng/ml of recombinant human EGF at 37°C. P-Tyr: phospho-tyrosine antibody; P-Akt: phospho-Akt antibody; Akt: Akt antibody; P-Erk1/2: phospho-Erk1/2 antibody; Erk2: Erk2 antibody. B) Melan-a cells WT or melan-a Ola-egfra stimulated for 30 minutes at 37°C with overnight conditioned supernatant of 293-T cells expressing vector alone, medaka egf, medaka areg or medaka hbegf. P-Tyr: phospho-tyrosine antibody; P-Akt: phospho-Akt antibody; Akt: Akt antibody; P-Erk1/2: phospho Erk1/2; Erk2: Erk2 antibody. The anti-P-Tyr antibody recognizes a band of the size of Egfra, thus indicating activated receptor.

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