Table 1 Plasmids and strains used in this study
From: Multiple genetic switches spontaneously modulating bacterial mutability
Plasmid or strain | Genotype and/or description | Reference of source |
|---|---|---|
pGEM-T easy | A-T cloning vector, ApR | Promega |
pHSG415 | temperature sensitive, CmR, KmR, ApR | Reference: [26] |
SGSC1417 | S. typhimurium LT7 non-mutator, wild type | Reference: [41] |
SGSC1412 | S. typhimurium LT2 non-mutator, wild type | Reference: [41] |
9052D1 | S. typhimurium LT7 mutator, genome unchanged, 6bpΔmutL | This study |
9052D1R | 9052D1, spontaneous mutL revertant | This study |
LT2mutLL1 | SGSC1412, mutL replaced by mutLLocked-1 | This study |
LT2mutLL2 | SGSC1412, mutL replaced by mutLLocked-2 | This study |
LT2mutLL3 | SGSC1412, mutL replaced by mutLLocked-3 | This study |
LT2mutLL4 | SGSC1412, mutL replaced by mutLLocked-4 | This study |
LT2mutLL1U | SGSC1412L1, mutLLocked-1 replaced by mutLL1-UL | This study |
LT2mutLLC | SGSC1412, mutL replaced by mutLLocked-C | This study |
LT26bpΔmutL | SGSC1412, mutL replaced by 6bpΔmutL | This study |
LT26bpΔmutLL | SGSC1412, mutL replaced by 6bpΔmutLLocked | This study |
LT2mutSL | SGSC1412, mutS replaced by mutSLocked | This study |