Multiple genetic switches spontaneously modulating bacterial mutability

Table 2 DNA repeats within mutL and mutS genes of S. typhimurium LT2 and synonymous substitution of selected bases

Gene	Location	DNA repeats^a	Allele	Substituted bases^b	Protein	Description
mutL	201-218	GCTGGCGCTGGCGCTGGCc	mutL ^Locked-1	GCT TGCCCTGGCGCTGGCc	LALALA	lies in a region that forms a lid over the ATP-binding pocket of MutL protein
			mutL ^L1-UL	GCT GGCGCTGGCGCTGGCc
	190-195	AAAAAA	mutL ^Locked-2	AA GAAA	KK	lies in a relatively disordered structure of ATP-binding pocket of MutL protein
	491-496	gAAAAAA	mutL ^Locked-3	gA GAAGA	EK	lies in α helic E of MutL protein
	735-743	CACCACCACg	mutL ^Locked-4	CAC GACTACg	TTT	lies in a relatively disordered structure of MutL C-terminal demonization domain
	82-90	AAAGAGCTG	mutL ^Locked-C	AAAGAGCTT	KEL	lies in the α helic A of MutL protein
6bpΔmutL	201-212	GCTGGCGCTGGc	6bpΔmutL^Locked	GCT TGCGCTGGCc	LALA	lies in a region that forms a lid over the ATP-binding pocket of MutL protein
mutS	1189-1195	AAAAAAA	mutS ^Locked	AA GAAAA	KK	lies in the C-terminal end of helic α16, which forms the core domain of the MutS protein

a, The first codon encoding the protein is underlined.
b, Italic characters represent modified bases; characters in small case indicate neighboring base of the sequence.

ISSN: 2730-7182