Molecular evolution and the role of oxidative stress in the expansion and functional diversification of cytosolic glutathione transferases

Table 5 Sites under positive selection detected by the BEB approach in PAML (bold, PP >0.9) and by ConTest (underlined, p < 0.05).

Alpha	Mu	Omega	Theta	Location	Amino acids	Experimentally confirmed functional relevance
12				interdomain interface	G, A, V, I, T
36				C-terminal domain interface	G, N, Q, K, E, H
	104			substrate binding pocket	A, V, L, I, T, F
103				substrate binding pocket	G, L, M, T, N, E
		128		substrate binding pocket	G, A, L, I, T, R
111				substrate binding pocket	V, L, I, T, F, Y , H	Residue 110 [46]
112					I, C, M, T, D, K	[46]
	126			interdomain interface	G, Q, R, K, E
	129			substrate binding pocket	Q, K, E
	130				G, A, T, S, Q, E
	166			substrate binding pocket	L, I, M, Q, R
			186	substrate binding pocket	V, L, E, K, R,
	192			N-terminal domain interface	K, E
			204	substrate binding pocket	A, S, K, R, E
208				substrate binding pocket	A, V, L, M, T, D, E, P
	210			interdomain interface	G, A, L, M, S, T, N	[30, 47]
212				interdomain interface	A, S, N, Q, K, Y	Residue 210 [46]
	214			substrate binding pocket	V, I, M, T, Q, K, F, H
			234	C-terminal	S, R	[48]
	218				I, M, T, N, K, R, E, D
			237	C-terminal	A, L, C, T, D

ISSN: 2730-7182