- Research article
- Open Access
Fitness costs associated with unnecessary virulence factors and life history traits: evolutionary insights from the potato late blight pathogen Phytophthora infestans
© Montarry et al; licensee BioMed Central Ltd. 2010
- Received: 24 February 2010
- Accepted: 16 September 2010
- Published: 16 September 2010
In gene-for-gene models of plant-pathogen interactions, the existence of fitness costs associated with unnecessary virulence factors still represents an issue, both in evolutionary biology and agricultural sciences. Measuring such costs experimentally has proven difficult, especially in pathogens not readily amenable to genetic transformation, since the creation of isogenic lines differing only by the presence or absence of avirulence genes cannot be achieved in many organisms. Here, we circumvented this difficulty by comparing fitness traits in groups of Phytophthora infestans isolates sharing the same multilocus fingerprint, but differing by their virulence/avirulence spectrum.
Fitness was assessed from calculations derived from the basic reproduction number, combining several life history traits (latent period, spore density and lesion growth rate) evaluated on leaflets of the potato cultivar Bintje, which is free of resistance genes. A statistically significant fitness cost was found in isolates virulent to the R10 resistance gene. That cost was due to a lower spore production in virulent isolates; however, the latent period was shorter in virulent isolates. Similar trends, although not statistically significant, were observed for the other genes tested.
The data likely reflect the adaptive response of the pathogen to the cost associated with virulence. They suggest strong trade-offs between life history traits related to pathogenicity and adaptive biology of pathogens.
- Virulent Isolate
- Fitness Cost
- Basic Reproduction Number
- Spore Density
- Avirulence Gene
Costs of adaptation play a major role in host-parasite coevolution, and thus take a central place in evolutionary theory. Many plant parasites interact with their hosts according to the widely accepted gene-for-gene model, which predicts that successful disease resistance is triggered only if a resistance (R) gene product in the host plant recognizes, directly or indirectly, a specific avirulence (Avr) gene product from the pathogen [1, 2]. In such systems, where resistance and pathogenicity (here termed virulence, according to Vanderplank ) are inherited as single genes , there are many recorded examples of directional selection leading to invasion of pathogen populations by virulent isolates, and hence resistance breakdown [e.g. [3, 5]]. However, such invasion has been slow in other cases [6, 7], suggesting that mutation from avirulence to virulence can have different fitness consequences depending on genes and populations.
A mutation from avirulence to virulence is always associated with an increase in fitness (i.e. the combined ability of an organism to survive and reproduce ) provided the R gene is present in at least part of the host population, because the virulent pathogens will be the only ones to exploit that fraction of the host population. However, mutation in an avirulence gene may also imply fitness costs, for instance if virulent pathogens perform less well (i.e. have a lower aggressiveness, or quantitative pathogenicity - see Pariaud et al. ) on hosts without the R-gene. Vanderplank  advocated this mechanism to explain that polymorphism for virulence can be maintained in pathogen populations exposed to hosts with R genes. Recent work  has shown that a cost of virulence increases the frequency of the corresponding resistance, creating frequency-dependent selection acting directly on host resistance or pathogen virulence, and would thus increase the value of the resistance gene in plant breeding.
An argument to explain that parasites would keep a molecule that allows them to be recognized by the host, and thus work as an avirulence effector, is that Avr proteins perform some essential function, and therefore must be retained . Avirulence genes from plant pathogenic viruses, bacteria and fungi do not share any strong structural homogeneity, but encode very diverse proteins with a range of demonstrated or postulated physiological functions [13–15]. Many of these genes are involved in life history traits which have a measurable effect on fitness, so that their mutations can impose a cost to virulence .
Fitness costs associated with unnecessary virulence factors were indeed detected in several plant pathogens. For instance, virulent strains of Xanthomonas oryzae pv. oryzae, obtained through mutagenesis, caused shorter lesions than the wild-type (avirulent) strain on a rice line containing no resistance genes . Similarly, a TuMV (Turnip Mosaic Virus) isolate avirulent on TuRB01 out-competed virulent isolates in co-inoculation experiments in a susceptible host background , suggesting a fitness cost to TuMV isolates overcoming resistance genes of Brassica crops. Detection of virulence fitness cost is possible in both agricultural and wild plant pathosystems, and in both controlled inoculation experiments and field surveys. For instance, Huang et al.  demonstrated that ascospores of the Brassica blackleg pathogen Leptosphaeria maculans avirulent to RLm4 produced more and larger lesions than virulent ascospores (avrLm4) on leaves of two Brassica napus cultivars lacking the corresponding resistance gene RLm4, and related that observation to the increasing frequency of AvrLm4 in two consecutive seasons of field experiments. In a similar way, Thrall and Burdon  described a trade-off between spore production and virulence in wild populations of the flax rust fungus Melampsora lini, so that selection favors virulent strains of M. lini in resistant Linum marginale populations and avirulent strains in susceptible populations. However, in many other cases, the detection of measurable fitness costs to virulence has been difficult or impossible , which suggests either that such costs can be low in some pathogens, or that these pathogens evolved compensatory mechanisms able to restore the fitness of virulent genotypes.
Two main approaches have been taken to evaluate the fitness cost imposed on pathogen isolates by mutation to virulence: to determine from population surveys whether virulent isolates decrease in frequency in the absence of the corresponding R-gene [21, 12], or to directly determine if inactivation of an avirulence gene implies a fitness penalty . Here, we used a third approach, similar to that of Bahri et al.  and consisting in comparing aggressiveness components in a near-isogenic collection of Phytophthora infestans (the potato late blight pathogen), i.e. in pathogen isolates showing the same allelic combination at ten microsatellite loci but polymorphic for virulence. Since French populations of P. infestans have been shown to be best adapted to the cultivar Bintje [24, 25], which is free of known race-specific resistance genes, we measured the performance of isolates on this cultivar.
While Bahri et al.  performed pairwise competition experiments to assess isolates' fitness, we measured classical aggressiveness components (latent period, spore density and lesion growth rate) in vitro on detached leaflets, and aggregated them into a fitness measure corresponding to the basic reproduction number (R0) in mathematical and evolutionary epidemiology.
Allelic combination at each of ten microsatellite loci of a quasi-isogenic collection of Phytophthora infestans isolates chosen to measure fitness costs associated with unnecessary virulence factors.
Virulence determination revealed 27 virulence phenotypes among the 132 quasi-isogenic isolates. The most common virulence profiles were 1-3-4-7-8-11 (i.e. isolates able to infect and sporulate on hosts possessing any combination of the race-specific resistance genes R1, R3, R4, R7, R8 and R11) (17%), 1-3-4-7-11 (17%), 1-3-4-7 (14%), 1-3-4-7-10-11 (10%), 1-3-4-7-8-10-11 (9%), 1-3-4-7-8 (7%), 1-3-4-7-10 (3%), 3-4-7 (3%) and 4-7 (2%). The other phenotypes were represented by only one or two isolates. Virulence phenotypes were usually complex, the mean number of virulence factors per isolate being 5. This allowed us to test the correlation between fitness and virulence complexity (ranging from two to eight virulence factors), and to compare the fitness of virulent and avirulent isolates for several virulence factors. That comparison was impossible for virulence to R2 and R5, because no isolate in the collection was virulent to either of these R-genes.
Fitness [as given by equation (1)] was computed for 1/μ = 80: the pathogen has 80 days to exploit a leaflet, which corresponds to the harvest or the natural leaflet death. The mean leaflet size X was set at 18 cm2, based on measures made on one hundred leaflets of the cultivar Bintje using a planimeter.
Mean values of aggressiveness components (LP - latent period, days; SD - spore density, or number of spores per cm2; LGR - lesion growth rate, in cm2per day), as measured on the potato cultivar Bintje.
Av (n = 20)
V (n = 112)
(F 1,130 ; P)
Av (n = 13)
V (n = 119)
(F 1,130 ; P)
Av (n = 5)
V (n = 127)
(F 1,130 ; P)
Av (n = 128)
V (n = 4)
(F 1,130 ; P)
Av (n = 5)
V (n = 127)
(F 1,130 ; P)
Av (n = 80)
V (n = 52)
(F 1,130 ; P)
Av (n = 94)
V (n = 38)
(F 1,130 ; P)
Av (n = 50)
V (n = 82)
(F 1,130 ; P)
The general objectives of this paper were to determine if fitness costs were associated with each of the main potato R-genes in Phytophthora infestans, and to identify the aggressiveness components concerned.
Fitness costs: why so few?
A fitness cost was detected for R10, and specifically explained by a lower spore density in virulent isolates. However, virulent isolates showed a shorter latent period than avirulent isolates. This unexpected observation will be discussed below. Regarding the other virulence genes, the situation was less clear. A consistent trend nevertheless existed for virulent isolates sporulating less, growing slower but having a shorter latent period than avirulent ones. The fact that virulence complexity (the number of virulence factors an isolate possesses) correlates with fitness raised the issue of whether the test would have been significant if the virulent and avirulent sample sizes had been more evenly balanced.
On the other hand, it is also possible that there is no virulence cost associated with all R-genes except R10. This hypothesis is coherent with recent molecular data on the distribution of avirulence genes in the P. infestans genome: avirulence genes (with the signature RxLR motif) are present in very high copy numbers in the genome , so a mutated copy (virulent) could be associated with a non-mutated copy (avirulent) which could assure the initial function of the gene. This hypothesis is also consistent with population data. Indeed, if a gene imposes a fitness cost, its frequency should decrease in natural pathogen populations, at least when cultivars carrying the corresponding R gene are absent or withdrawn; conversely, virulent factors which are not costly can persist in populations. Andrivon  and Lebreton et al.  found the same complex virulence phenotypes (i.e. 18.104.22.168.8.11, 22.214.171.124.11 and 126.96.36.199) to dominate in P. infestans populations collected in French potato production areas, despite the absence of local selection by the corresponding R genes . That stability of virulence phenotypes over a long period, at the French but also at a world-wide spatial scale [32, 33], led to hypothesize that most phenotypes carry "fossil virulences" , which do not reflect local selection but selection in the pathogen's center of origin, prior to migration.
Another possibility, that Bahri et al.  also mentioned for the yellow rust pathogen Puccinia striiformis, is that virulence genes are not selected against because costs are compensated. Schoustra et al. , working with the filamentous ascomycete Aspergillus nidulans, found that fludioxonil-resistant isolates presented a severe fitness cost in the absence of the drug, but that this cost was afterwards waived without loss of the resistance. More generally, compensatory mutations have been detected, often in a fortuitous way, during genetic analyses of different viruses of animals or plants (see Luna et al.  and references therein).
Fitness and aggressiveness components: an evolutionary explanation for an apparent biological contradiction?
There remains to be explained why virulent isolates often have a significantly lower latent period. A possible interpretation is the following. Let us assume the pathogen has to allocate host resources to either growth (mycelium) or reproduction (spores), and that possessing a virulence gene makes it more difficult to make mycelium. Then, reinvesting some resources otherwise allocated to mycelium into spore production would make sense, and would reduce the time to make a spore (the latent period). In other words, the shorter latent period in virulent isolates might well reflect an adaptive response from the pathogen to the cost of virulence. This question now merits to be analyzed in a more generic way, using both a theoretical modeling approach (e.g. ) and the analysis of experimental data from a range of pathosystems, including biotrophs, hemibiotrophs and necrotrophs.
Fitness costs: small, but cumulative
A previous report on P. infestans indicated that race complexity (i.e. the number of R genes an isolate is able to overcome) had no effect on aggressiveness of isolates collected in Germany and in the Netherlands: laboratory and field tests did not show any decreased fitness of the more complex races compared to less complex races or to race 0 . By contrast, our data, gathered on genetically related isolates, showed that fitness was negatively correlated with virulence complexity. This suggests that i) the small but consistent differences in aggressiveness observed for each individual gene are indeed cumulative, and may end-up in selection against the most complex races, and that ii) the rest of the pathogen genome (i.e. genes not directly related to pathogenicity) could be involved in the restoration of fitness costs due to unnecessary virulence.
Interestingly, a quadratic and concave regression model fitted the data as well as a linear model (Figure 1), suggesting that the marginal loss of fitness increases with every additional virulence. This finding, as observed earlier in a bacterial pathogen after serial knock-out of effector-coding genes , is consistent with theoretical multi-locus models explaining the persistence of polymorphisms in gene-for-gene interactions, and promotes diversity in resistance and avirulence genes . It could thus explain the stability of polymorphisms in P. infestans populations, although these are dominated by clonal lineages.
Having such insights on the way virulent isolates respond to fitness costs may help making more durable the management of resistance genes. There is now both theoretical and experimental evidence supporting the idea to combine race-specific and quantitative resistance to enhance the durability of major genes [41–43]. Furthermore, we have shown that the fitness cost to the pathogen increases with the number of virulences, which could be an argument to build R-gene pyramids and benefit from a lower fitness of multi-virulent isolates (i.e. residual effects of defeated R-genes ). The cost of virulence to R10 could have been exploited in a such a pyramid of several R genes, or in genotypes combining R10 with high levels of partial resistance. Unfortunately, this is no longer possible, because R10 was originally introduced alone in susceptible genetic backgrounds, and has thus selected for virulent isolates that now constitute a very large fraction of the pathogen populations in most parts of the world (see for instance [32, 33]). However, it points to the fact that race-specific resistance genes should be protected by such a combining strategy as soon as they are introduced into breeding material.
P. infestans belongs to the Oomycetes, a group of filamentous protists closely related to the brown algae . P. infestans can infect all parts of the potato plant (i.e. shoots, stems, leaves, berries and tubers), leading to serious yield losses. It is characterized by a primarily aerial life cycle  with large multiplication rates  and polycyclic epidemics favoring rapid response to selection, which may however be counterbalanced by the large dispersion capacity of the asexual sporangia containing the infective zoospores . P. infestans is a heterothallic species: sexual reproduction requires the simultaneous presence of hyphae of the two opposite mating types, designated A1 and A2 . While infected tubers are the most common source of inoculum at the beginning of the season in temperate climates , infections can also start from oospores that result from the sexual cycle.
Phytophthora infestans isolates were collected during two consecutive years (2004 and 2005) in the two major French potato production areas, Brittany and northern France. Axenic cultures were established from 596 isolates. Each single-lesion isolate was obtained by placing 1 cm2 pieces of infected tissue on tuber slices of potato cultivar Bintje, kept in plastic trays for six days at 15°C. Pure axenic cultures were then obtained by transferring small pieces of mycelium growing on the upper side of the potato slice to pea agar supplemented with 200 mg.L-1 ampicillin, 30 mg.L-1 rifamycin, and 0.4 ml.L-1 pimaricin. After approximately 10 days at 15°C, growing colonies were transferred to pea agar without antibiotics, and subsequently maintained by serial transfers.
Each of the 596 isolates of P. infestans was grown separately in pea broth autoclaved for 20 min at 120°C. After 10 to 15 days of incubation at 18°C, mycelium was washed three times in sterile water, and lyophilized. DNA was extracted as described by Lebreton et al.  and stored in TE buffer containing 10 M Tris-HCL and 0.1 M EDTA (pH 8.0). DNA concentration and purity were estimated using a spectrofluorimeter (SpectraMax M2).
Alleles at 10 polymorphic microsatellite loci - Pi4B, Pi4G and PiG11 developed by Knapova and Gisi ; and Pi02, Pi04, Pi16, Pi33, Pi56, Pi63 and Pi70 developed by Lees et al.  - were amplified in Polymerase Chain Reactions (PCR) performed in a 12.5 μL volume containing between 20 and 200 ng of DNA of P. infestans, 2.5 μL of 5× PCR Buffer (Promega), 0.3 mM of each dNTP, 2.5 mM of MgCl2 (Promega), 0.3 μM each of forward and reverse primers, and 1.25 U of Taq DNA polymerase (GoTaq® flexi DNA polymerase, Promega). PCRs were performed in a MJ Research thermocycler under the following conditions: each reaction started with a cycle of 2 min at 95°C, followed by 30 cycles of 20 s at 95°C, 25 s at 56°C (for PiG11), 58°C (for Pi02, Pi04, Pi16, Pi33, Pi56, Pi63, Pi70 and Pi4B) or 60°C (for Pi4G) and 60 s at 72°C, and finished with an elongation cycle of 5 min at 72°C. In order to detect simultaneously the alleles at several loci, primers were labeled with one of three fluorescent dyes: FAM (PiG11, Pi33, Pi 63, Pi70, Pi02, and Pi4B), NED (Pi56, Pi04, Pi4G) and HEX (Pi16). Amplification products were pooled into three groups, based on expected allele sizes: PiG11, Pi56 and Pi33; Pi63, Pi04, and Pi70; and Pi02, Pi4G, Pi16 and Pi4B, respectively. Ten μL samples, comprising 9.84 μL of deionized formamide Hi-Di™ (Applied Biosystems), 0.06 μL of 400 HD ROX™ Size standard (Applied Biosystems), and 0.1 μL of PCR multiplexed product, were loaded into an ABI Prism 3130xl DNA sequencer run according to manufacturer's instructions (Applied Biosystems). DNA fragments were automatically sized with the GeneMapper™ 3.5 software. Allele sizes were calibrated to the allele sizes of reference isolates kindly provided by Drs Lees and Cooke of the Scottish Crop Research Institute, UK .
Potato plant material
Plants of the potato cultivar Bintje were grown from certified seed tubers in 13 cm pots (one tuber per pot) filled with 1:1:1 sand-peat-compost mixture, in a glasshouse regulated at 15-20°C (night/day temperatures) and 16 h of photoperiod. Plants were watered with a nutrient solution (Hakaphos; NPK 15/10/15) once a week.
Sporangial suspensions of each of the 132 isolates of the quasi-isogenic collection were prepared from 20-days-old cultures on pea agar, and adjusted to 5.104 sporangia per mL using a haemacytometer. Before inoculation, sporangial suspensions were kept at 4°C for approximately 4 hours to promote zoospore liberation.
Virulence phenotype determination
Virulence patterns were determined using the international late blight differential set of potato clones, each having one of the R1-R11 race-specific resistance genes, and 2 susceptible cultivars (Bintje and Craigs Royal). Recent work has shown that the R3 differential (CEBECO-4642-1) actually contains two closely linked genes, R3a and R3b . Virulence to each of these could not be assessed separately, due to the lack of available differential hosts with only one of these two genes. Therefore, virulence to R3 was regarded as a single factor. Virulence to R9 could not be assessed because the corresponding differential clone was not present in our test set; to our knowledge, this gene has never been introduced into commercial European cultivars. Since the physiological age of plants can affect the expression of resistance genes , all tests were performed using leaflets detached from 6-8-week-old plants. Each leaflet was placed abaxial face up on a moist filter paper in a clear plastic dish, and inoculated by depositing a 20 μL drop of the sporangial suspension (containing ca. 1000 spores) on each side of the midrib. Two leaflets per isolate and differential host were inoculated. Dishes containing the inoculated leaflets were deposited in clear plastic boxes, and incubated in an illuminated incubator for 7 days at 18°C/15°C day/night with 16 h day length. After incubation, each inoculation site was scored for the presence or absence of a sporulating lesion. This method has proved reliable to assess virulence of a range of diversified isolates in a ring test across 12 European laboratories .
Each isolate was tested for aggressiveness on the cultivar Bintje. Six leaflets were detached from 6-8-week-old plants, and placed abaxial face up on the lids of inverted Petri dishes containing 12 g.L-1 water agar (two leaflets per dish). Each leaflet was inoculated by depositing a 20 μL drop of the sporangial suspension as close as possible to the leaflet centre. Inoculated leaflets were then incubated as described for the virulence tests. The latent period (LP) was determined by observing daily the appearance of sporangia. Seven days after inoculation, lesion diameters were measured in two perpendicular directions and lesion size was computed assuming an elliptic shape. Then, leaflets were washed in 10 mL Isoton II (saline buffer), and the sporangia production per leaflet was determined with a Coulter Z2 counter (Beckman Coulter France, Villepinte, France). This allowed measuring the spore density (SD), i.e. the number of sporangia produced per cm2 of lesion, and assessing the lesion growth rate (LGR), assumed constant in cm2 per day . More accurately, the lesion size was divided by the time elapsed since it started growing, at time LP (the lesion represents the area having sporulated).
An epidemiological model was developed to derive the basic reproduction number of the pathogen (R0, equal to the number of secondary infections generated by each lesion). This allowed to aggregate measures made on leaflets (LP, SD and LGR) into a single fitness estimate.
where B is the "birth" rate and 1/μ represents the time available to the pathogen to exploit a leaflet (since harvest or natural death make it finite). The three epidemiological parameters, denoted β, λ and α are defined as the transmission rate, the latent period and the inverse of the infectious period, respectively. Clearly, λ = LP. Let X be the underlying leaflet size (a constant) in this model. Since α is the inverse of the time actually taken to exploit a leaflet after the latent period has elapsed, α = LGR/X. To estimate β, let Y be the probability for a released spore to come in contact with a particular host times the infection success rate. Then, β = Y. σ, where σ is the number of sporangia produced per unit time per infectious host (see Madden et al. ), that is, σ = SD.LGR.
The effect of virulence/avirulence (vir/avir effect) on the aggressiveness components (LP, SD and LGR) and on the fitness (F) distributions was tested for each R-gene through a one-way ANOVA using the GLM procedure of the SAS statistical software, v. 8.1 (SAS Institute, Cary, NC). The Shapiro-Wilk test showed that residuals have a normal distribution. The fitness value was calculated for each of the 132 isolates from the experimental data, using fixed values of X (18 cm2, mean leaflet size in cv Bintje) and 1/μ (80 days, time available to the pathogen to exploit a leaflet since harvest or natural death). Additionally, the relationship between fitness (F) or aggressiveness components (LP, SD and LGR) and virulence complexity (i.e. the number of R-genes overcome by the isolate) was tested with the Spearman's rank correlation rho using the statistical freeware R, version 2.9.2 (R Development Core Team 2009), to detect compensatory or additive effects of costs from different virulence factors. The model selection based on Akaike's information criteria, AIC , was used in order to determine which parameterized model (linear or quadratic) best fitted the data.
The authors thank E. Lesne for establishing isolate collections, H. Douchy and B. Marquer for participating in the biological tests, and G. Mialdéa for help with the molecular characterization. The molecular characterization was performed on the Biogenouest® facilities. Financial support from ACVNPT (Association des Créateurs de Variétés Nouvelles de Pomme de Terre) and from the European Integrated Project BioEXPLOIT [Contract 513959 (FOOD)] are gratefully acknowledged. This work is part of an INRA-BBSRC funded project entitled 'Epidemiological and evolutionary models for invasion and persistence of plant diseases.' FMH also acknowledges partial funding from Agropolis Fondation and the INRA SPE Department. We are very grateful to Dr. Frédéric FABRE and to two anonymous referees for valuable comments on a previous version of this paper.
- Flor HH: The complementary genic systems in flax and flax rust. Adv Genet. 1956, 8: 29-54. full_text.View ArticleGoogle Scholar
- Flor HH: Current status of gene-for-gene concept. Annu Rev Phytopathol. 1971, 9: 275-296. 10.1146/annurev.py.09.090171.001423.View ArticleGoogle Scholar
- Vanderplank JE: Plant Diseases: Epidemics and Control. 1963, New York: Academic PressGoogle Scholar
- Pedley KF, Martin GB: Molecular basis of Pto-mediated resistance to bacterial speck disease in tomato. Annu Rev Phytopathol. 2003, 41: 215-243. 10.1146/annurev.phyto.41.121602.143032.View ArticlePubMedGoogle Scholar
- Wolfe MS: Can the strategic use of disease resistant hosts protect their inherent durability?. Durability of Disease Resistance. Edited by: Jacobs T, Parlevliet JE. 1993, Dordrecht: Kluwer Academic Publishers, 83-96.View ArticleGoogle Scholar
- Johnson R: Classical plant breeding for durable resistance to diseases. J Plant Pathol. 2000, 82: 3-7.Google Scholar
- Boyd LA: Can the durability of resistance be predicted?. J Sci Food Agr. 2006, 86: 2523-2526. 10.1002/jsfa.2648.View ArticleGoogle Scholar
- Crow JF: Basic concepts in population, quantitative, and evolutionary genetics. 1986, New York: Academic PressGoogle Scholar
- Pariaud B, Ravigné V, Halkett F, Goyeau H, Carlier J, Lannou C: Aggressiveness and its role in the adaptation of fungal plant pathogens. Plant Pathol. 2009, 58: 409-424. 10.1111/j.1365-3059.2009.02039.x.View ArticleGoogle Scholar
- Vanderplank JE: Disease Resistance in Plants. 1968, New York: Academic PressGoogle Scholar
- Tellier A, Brown JKM: Stability of genetic polymorphism in host-parasite interactions. Proc Royal Soc London B- Biol Sci. 2007, 274: 809-817. 10.1098/rspb.2006.0281.View ArticleGoogle Scholar
- Vera-Cruz CM, Bai J, Ona I, Leung H, Nelson RJ, Mew TW, Leach JE: Predicting durability of a disease resistance gene based on an assessment of the fitness loss and epidemiological consequences of avirulence gene mutation. Proc Natl Acad Sci USA. 2000, 97: 13500-13505. 10.1073/pnas.250271997.PubMed CentralView ArticlePubMedGoogle Scholar
- White FF, Yang B, Johnson LB: Prospects for understanding avirulence gene function. Curr Opin Plant Biol. 2000, 3: 291-298. 10.1016/S1369-5266(00)00082-0.View ArticlePubMedGoogle Scholar
- Luderer R, Joosten MHAJ: Avirulence proteins of plant pathogens: determinants of victory and defeat. Mol Plant Pathol. 2001, 2: 355-364. 10.1046/j.1464-6722.2001.00086.x.View ArticlePubMedGoogle Scholar
- Skamnioti P, Ridout CJ: Microbial avirulence determinants: guided missiles or antigenic flak?. Mol Plant Pathol. 2005, 6: 551-559. 10.1111/j.1364-3703.2005.00302.x.View ArticlePubMedGoogle Scholar
- Leach JE, Cruz CMV, Bai JF, Leung H: Pathogen fitness penalty as a predictor of durability of disease resistance genes. Annu Rev Phytopathol. 2001, 39: 187-224. 10.1146/annurev.phyto.39.1.187.View ArticlePubMedGoogle Scholar
- Bai J, Choi SH, Ponciano G, Leung H, Leach JE: Xanthomonas oryzae pv. oryzae avirulence genes contribute differently and specifically to pathogen aggressiveness. Mol Plant-Microbe Interact. 2000, 13: 1322-1329. 10.1094/MPMI.2000.13.12.1322.View ArticlePubMedGoogle Scholar
- Jenner CE, Wang X, Ponz F, Walsh JA: A fitness cost for Turnip mosaic virus to overcome host resistance. Virus Res. 2002, 86: 1-6. 10.1016/S0168-1702(02)00031-X.View ArticlePubMedGoogle Scholar
- Huang YJ, Li ZQ, Evans N, Rouxel T, Fitt BDL, Balesdent MH: Fitness cost associated with loss of the AvrLm4 avirulence function in Leptosphaeria maculans (phoma stem canker of oilseed rape). Eur J Plant Pathol. 2006, 114: 77-89. 10.1007/s10658-005-2643-4.View ArticleGoogle Scholar
- Thrall PH, Burdon JJ: Evolution of virulence in a plant host-pathogen metapopulation. Science. 2003, 299: 1735-1737. 10.1126/science.1080070.View ArticlePubMedGoogle Scholar
- Leung H, Nelson RJ, Leach JE: Population structure of plant pathogenic fungi and bacteria. Advances in Plant Pathology. Edited by: Andrews JH, Tommenup IC. 1993, New York: Academic Press, 10: 157-205.Google Scholar
- Burdon JJ: Genetic variation in pathogen populations and its implications for adaptation to host resistance. Durability of Disease Resistance. Edited by: Jacobs T, Parlevliet JE. 1993, Dordrecht: Kluwer Academic Publishers, 41-56.View ArticleGoogle Scholar
- Bahri B, Kaltz O, Leconte M, de Vallavieille-Pope C, Enjalbert J: Tracking costs of virulence in natural populations of the wheat pathogen, Puccinia striiformis f. sp. tritici. BMC Evol Biol. 2009, 9: 26-10.1186/1471-2148-9-26.PubMed CentralView ArticlePubMedGoogle Scholar
- Andrivon D, Pilet F, Montarry J, Hafidi M, Corbière R, Achbani EH, Pellé R, Ellissèche D: Adaptation of Phytophthora infestans to partial resistance in potato: Evidence from French and Moroccan populations. Phytopathology. 2007, 97: 338-343. 10.1094/PHYTO-97-3-0338.View ArticlePubMedGoogle Scholar
- Montarry J, Glais I, Corbiere R, Andrivon D: Adaptation to the most abundant host genotype in an agricultural plant-pathogen system - potato late blight. J Evol Biol. 2008, 21: 1397-1407. 10.1111/j.1420-9101.2008.01557.x.View ArticlePubMedGoogle Scholar
- Arnaud-Haond S, Belkhir K: GENCLONE: a computer program to analyse genotypic data, test for clonality and describe spatial clonal organization. Mol Ecol Notes. 2007, 7: 15-17. 10.1111/j.1471-8286.2006.01522.x.View ArticleGoogle Scholar
- Montarry J, Andrivon D, Glais I, Corbière R, Mialdea G, Delmotte F: Microsatellite markers reveal two admixed genetic groups and an ongoing displacement within the French population of the invasive plant pathogen Phytophthora infestans. Mol Ecol. 2010, 19: 1965-1977. 10.1111/j.1365-294X.2010.04619.x.View ArticlePubMedGoogle Scholar
- Haas BJ, Kamoun S, Zody MC, Jiang RHY, Handsaker RE, Cano LM, Grabherr M, Kodira CD, Raffaele S, Torto-Alalibo T, et al: Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature. 2009, 461: 393-398. 10.1038/nature08358.View ArticlePubMedGoogle Scholar
- Andrivon D: Races of Phytophthora infestans in France, 1991-1993. Potato Res. 1994, 37: 279-286. 10.1007/BF02360520.View ArticleGoogle Scholar
- Lebreton L, Laurent C, Andrivon D: Evolution of Phytophthora infestans populations in the two most important potato production areas in France during 1992-1996. Plant Pathol. 1998, 47: 427-439. 10.1046/j.1365-3059.1998.00265.x.View ArticleGoogle Scholar
- Andrivon D, Corbière R, Lebreton L, Pilet F, Montarry J, Pellé R, Ellissèche D: Host adaptation in Phytophthora infestans: a review from a population biology perspective. Plant Breed Seed Sci. 2004, 50: 15-27.Google Scholar
- Andrivon D: Race structure and dynamics in populations of Phytophthora infestans. Can J Bot. 1994, 72: 1681-1687. 10.1139/b94-206.View ArticleGoogle Scholar
- Derie ML, Inglis DA: Persistence of complex virulences in populations of Phytophthora infestans in Western Washington. Phytopathology. 2001, 91: 606-612. 10.1094/PHYTO.2001.91.6.606.View ArticlePubMedGoogle Scholar
- Andrivon D, de Vallavieille-Pope C: Race diversity and complexity in selected populations of fungal biotrophic pathogens of cereals. Phytopathology. 1995, 85: 897-905. 10.1094/Phyto-85-897.View ArticleGoogle Scholar
- Schoustra SE, Debets AJM, Slakhorst M, Hoekstra RF: Reducing the cost of resistance; experimental evolution in the filamentous fungus Aspergillus nidulans. J Evol Biol. 2006, 19: 1115-1127. 10.1111/j.1420-9101.2006.01102.x.View ArticlePubMedGoogle Scholar
- Luna E, Rodriguez-Huete A, Rincon V, Mateo R, Mateu MG: A systematic study of the genetic response of a variable virus to the introduction of deleterious mutations in a functional capsid region. J Virol. 2009, 83: 10140-10151. 10.1128/JVI.00903-09.PubMed CentralView ArticlePubMedGoogle Scholar
- van den Berg F, Bacaer N, Metz JAJ, Lannou C, van den Bosch F: Periodic host absence can select for higher or lower parasite transmission rates. Evol Ecol. 2010Google Scholar
- Schöber B, Turkensteen LJ: Recent and future developments in potato fungal pathology. Neth J Plant Pathol. 1992, 98 (suppl 2): 73-83. 10.1007/BF01974474.View ArticleGoogle Scholar
- Wichmann G, Bergelson J: Effector genes of Xanthamonas axonopodis pv. vesicatoria promote transmission and enhance other fitness traits in the field. Genetics. 2004, 166: 693-706. 10.1534/genetics.166.2.693.PubMed CentralView ArticlePubMedGoogle Scholar
- Tellier A, Brown JKM: Polymorphism in multilocus host-parasite coevolutionary interactions. Genetics. 2007, 177: 1777-1790. 10.1534/genetics.107.074393.PubMed CentralView ArticlePubMedGoogle Scholar
- Palloix A, Ayme V, Moury B: Durability of plant major resistance genes to pathogens depends on the genetic background, experimental evidence and consequences for breeding strategies. New Phytol. 2009, 183: 190-199. 10.1111/j.1469-8137.2009.02827.x.View ArticlePubMedGoogle Scholar
- Brun H, Chevre AM, Fitt B, Powers S, Besnard AL, Ermel M, Huteau V, Marquer B, Eber F, Renard M, Andrivon D: Quantitative resistance increases the durability of qualitative resistance to Leptosphaeria maculans in Brassica napus. New Phytol. 2010, 185: 285-299. 10.1111/j.1469-8137.2009.03049.x.View ArticlePubMedGoogle Scholar
- McDonald B: How can we achieve durable disease resistance in agricultural ecosystems?. New Phytol. 2010, 185: 3-5. 10.1111/j.1469-8137.2009.03108.x.View ArticlePubMedGoogle Scholar
- Pedersen WL, Leath S: Pyramiding major genes for resistance to maintain residual effects. Annu Rev Phytopathol. 1988, 26: 369-378. 10.1146/annurev.py.26.090188.002101.View ArticleGoogle Scholar
- Kroon LPNM, Bakker FT, van den Bosch GBM, Bonants PJM, Flier WG: Phylogenetic analysis of Phytophthora species based on mitochondrial and nuclear DNA sequences. Fungal Genet Biol. 2004, 41: 766-782. 10.1016/j.fgb.2004.03.007.View ArticlePubMedGoogle Scholar
- Alexopoulos CJ, Mims CW, Blackwell M: Phytophthora infestans. Introductory Mycology. 1994, John Wiley and Sons, 717-720. 4Google Scholar
- Harrison JG: Effects of the aerial environment on late blight of potato foliage - a review. Plant Pathol. 1992, 41: 384-416. 10.1111/j.1365-3059.1992.tb02435.x.View ArticleGoogle Scholar
- Bourke PMA: Emergence of potato blight, 1843-46. Nature. 1964, 203: 805-808. 10.1038/203805a0.View ArticleGoogle Scholar
- Smoot JJ, Gough FJ, Lamey HA, Eichenmuller JJ, Gallegly ME: Production and germination of oospores of Phytophthora infestans. Phytopathology. 1958, 48: 165-171.Google Scholar
- Zwankhuizen MJ, Govers F, Zadoks JC: Development of potato late blight epidemics: disease foci, disease gradients and infection sources. Phytopathology. 1998, 88: 754-763. 10.1094/PHYTO.19188.8.131.524.View ArticlePubMedGoogle Scholar
- Knapova G, Gisi U: Phenotypic and genotypic structure of Phytophthora infestans populations on potato and tomato in France and Switzerland. Plant Pathol. 2002, 51: 641-653. 10.1046/j.1365-3059.2002.00750.x.View ArticleGoogle Scholar
- Lees AK, Wattier R, Shaw DS, Sullivan L, Williams NA, Cooke DEL: Novel microsatellite markers for the analysis of Phytophthora infestans populations. Plant Pathol. 2006, 55: 311-319. 10.1111/j.1365-3059.2006.01359.x.View ArticleGoogle Scholar
- Huang SW, Vleeshouvers V, Werij JS, Hutten RCB, van Eck HJ, Visser RGF, Jacobsen E: The R3 resistance to Phytophthora infestans in potato is conferred by two closely linked R genes with distinct specificities. Mol Plant-Microbe Interact. 2004, 17: 428-435. 10.1094/MPMI.2004.17.4.428.View ArticlePubMedGoogle Scholar
- Stewart HE: Effect of plant age and inoculum concentration on expression of major gene resistance to Phytophthora infestans in detached potato leaflets. Mycol Res. 1990, 94: 823-826. 10.1016/S0953-7562(09)81385-3.View ArticleGoogle Scholar
- Andrivon D, Avendaño-Córcoles J, Cameron AM, Carnegie SF, Cooke LR, Corbière R, Detourné D, Dowley LJ, Evans D, Forisekova K, Griffin DG, Hannukkala A, Lees AK, Lebecka R, Niepold F, Polgar Z, Shaw DS, Thompson J, Trognitz B, van Raaij HMG, Zimnoch-Guzowska E: Virulence of Phytophthora infestans: its stability and variability assessed in a ring test across European laboratories. Plant Pathol. Google Scholar
- Latijnhouwers M, Ligterink W, Vleeshouwers VGAA, Van West P, Govers F: A G-alpha subunit controls zoospore motility and virulence in the potato late blight pathogen Phytophthora infestans. Mol Microbiol. 2004, 51: 925-936. 10.1046/j.1365-2958.2003.03893.x.View ArticlePubMedGoogle Scholar
- Brauer F: Compartmental models in epidemiology. Matematical epidemiology. Lecture Notes in Matematics. Edited by: Brauer et al. 2008, Springer series, 1945: 19-79.Google Scholar
- Madden LV, Hughes G, van den Bosch F: The study of plant disease epidemics. 2007, St Paul, MN: APS PressGoogle Scholar
- van den Driessche P, Watmough J: Further notes on the basic reproduction number. Matematical epidemiology. Lecture Notes in Matematics. Edited by: Brauer et al. 2008, Springer series, 1945: 81-130.Google Scholar
- Diekmann OA: A beginners guide to adaptive dynamics. Mathematical modeling of population dynamics. 2004, Warszawa: Banach Center Publications, Institute of Mathematics, Polish Academy of Sciences, 63: 47-86.Google Scholar
- Johnson JB, Omland KS: Model selection in ecology and evolution. Trends Ecol Evol. 2004, 19: 101-108. 10.1016/j.tree.2003.10.013.View ArticlePubMedGoogle Scholar
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