Table 2 Primer pairs and annealing temperatures successfully used for primer walking.
Primer Pair | Primer Sequence(3'-5') | Annealing Temperature(°C) |
|---|---|---|
SR 16S-f | CTT ATG TTT TTT TTA GTT TGC GAC CTC | 62 |
SR cob-f | GGG GGA GTG ATT GCT TTG TTG C | 64 |
SR cox3-f | CAA CAG GGT TTC ACG GAA TAC ACG | 65 |
SR 16S-r | GAG GTC GCA AAC TAA AAA AAA CAT AAG | 62 |
SR cobf-rev | TGT TGG AGA TCT AAC AGA ATA AGC AC | 60.1 |
SR co1f-rev | CTT TCT GAG ATA AAA GTA GGT CCT GG | 61.6 |