Successfully resisting a pathogen is rarely costly in Daphnia magna

Table 2 Details of experimental designs presented in the current study.

			Exposure					Food
#	Host clones	Parasite strains	spore nb	nb	time (days)	age (days)	Jar size (mL)	Sp.	qty (×10⁶)	T°(°C)	rep	ind/jar	Cont.	Expe time (Days)
1	GG3,GG4, GG7,GG13	Sp1,Sp8	50000	1	2	5	60	C	3.5	20	35	1	c. D.	last death
		Sp1	200000
2	GG4,GG7	Sp1	20000	1, 2, 4	2	5, 11, 19, 27	60	C	3.5	20	40	1	c. D.	last death
3	GG4	Sp1	2500	1, 2	10^a	1, 5^a	60	C	2.1	15, 20,25	30	1	c. D. H₂O	60 days^a
4	GG3	Sp1	5000	1	5	1	60	S	5	20	24/72	1	c. D.	38 days
			50000
5	<20 clones, recently wild-caught^b	Mix wild spores	50000	1	5	1	200	S	5	20	70	5	-	35
			100000

^aat 20°C, degree-day equivalent for other T°
^b Hosts were collected from a Scottish population in Summer 2003, see Duncan and Little Evolution 2007, 61(4):796-803
For each experiment (referred to by their number: #), the D. magna clones (Host clones) and the P. ramosa strains (Parasite strains) used are indicated. The protocol used for the exposure(s) is given, described by the number of spores per Daphnia added (spore nb), the number (nb), the length (time) and the age (age) of the individuals when each exposure was performed. The environmental conditions of the experiment are detailed. For food, the algae species (Sp.: C for Chlorella, S for Scenedesmus) and the quantity (qty, in millions of cells) are indicated. The temperature(s) at which the experiment was performed (T°), the number of replicates per treatment (rep), the number of individuals per jar (ind/jar), the jar size, the controls used [Cont.: crushed unexposed Daphnia (c. D.) or sterile water (H₂O)] and the total length of the experiment (Expe time) are also given.

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