Metazoan Wnt genes. The Wnt subfamilies (1 to 11, 16 and A) found in the various metazoans are represented by coloured boxes. Grey boxes indicate the loss of particular Wnt subfamilies and boxes with question marks indicate Wnts not found in some animals, but which cannot be definitively described as 'lost' because the relevant genomes have not been sequenced or require more comprehensive annotation. Duplicated Wnts are represented by two overlapping boxes. Note that Wnt8 is also called WntD in Drosophila and Tribolium. The phylogenetic relationships of the various animals is indicated by the tree on the left [14–16, 21]. The asterisk indicates that for WntA an orthologue was isolated from another spider, Cupiennius. Note that the complete Achaearanea Wnt6 sequence was only identified subsequent to the phylogenetic analysis.