Expression of Wnt genes in Glomeris. Stage 3 embryo double stained for Wnt6 and en (a). Higher magnification of ventral trunk segments T1 to T3 of same embryo in (a) showing abutting expression of Wnt6 and en (b). Expression of Wnt16 and en in a stage 3 embryo (c). Higher magnification of ventral trunk segments T1 to T3 of same embryo in (c) showing abutting segmental expression of Wnt16 and en (d). Expression of WntA and en in a stage 5 embryo (e). Higher magnification of ventral trunk segments T1 to T4 of same embryo in (e) showing abutting segmental expression of WntA and en (f). Stage 5 embryo stained for Wnt7 (g). Arrow indicates expression in the midgut. Note that Gm-Wnt7 expression appears to be restricted to embryos older than approximately stage 3. Expression of Wnt8 in a stage 0.3 embryo (h). Faint expression of Gm-Wnt8 at the posterior is out of focus in this picture. Coloration in the middle of the embryo is in the yolk; this artificial staining appears when over-staining Glomeris embryos, which was necessary to detect specific Wnt8 transcripts. Note that Gm-Wnt8 expression appears to be restricted to embryos younger than approximately stage 1. Expression of Wnt11 in a stage 0.3 embryo (i) and a stage 3 embryo (j). Expression of Gm-Wnt11 is restricted to the anal valves (av) and the growing appendages (i), (j). Expression of Glomeris wg is described elsewhere [32, 33]. All embryos are shown with anterior to the left. Embryos in (a) to (f) are flat mounted. Abbreviations: an, antennal segment; av, anal valve; lb, labrum; md, mandibulary segment; mx, maxillary segment; OC, optic lobes; pmd, pre-mandibulary segment; pmx, post-maxillary segment; T1-T4, trunk segments one to four.