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Table 1 Characteristics of adaptive substitutions in the D promoter of ϕX174 that arose in previous evolution experiments.

From: Positive selection at high temperature reduces gene transcription in the bacteriophage ϕX174

Strain

Mutation

Change in Protein C

# of Exps

Host*

Temperature, °C*

mut319

G 319 T

V63F

4**

Salmonella typhimurium

43.5

   

2, 1

Escherichia coli

37, 43.5

mut321

T 321 C

none

1

Salmonella typhimurium

43.5

mut323

A 323 G

N64G

3

Salmonella typhimurium

43.5

   

1

Escherichia coli

43.5

mut324

C 324 T

none

1

Shigella sonnei

42

   

1

Escherichia coli

43.5

  1. *Conditions under which substitutions arose [HA Wichman, pers. comm.; [3538]]
  2. ** Also, one reversion after switch to E. coli host at 43.5°C.