Table 4 Oligonucleotides used for site-directed mutagenesis, allele-specific PCR and quantitative PCR
From: Positive selection at high temperature reduces gene transcription in the bacteriophage ϕX174
Primer Name | Location* | Sequence (5' to 3') | Purpose |
|---|---|---|---|
319F | 314-338 | CTCTTTTTGACATTTTAAAAGAGCG** | Mutagenesis |
319R | 338-314 | CGCTCTTTTAAAATGTCAAAAAGAG | Mutagenesis |
321F | 314-338 | CTCTTGTCGACATTTTAAAAGAGCG | Mutagenesis |
321R | 338-314 | CGCTCTTTTAAAATGTCGACAAGAG | Mutagenesis |
323F | 314-338 | CTCTTGTTGGCATTTTAAAAGAGCG | Mutagenesis |
323R | 338-314 | CGCTCTTTTAAAATGCCAACAAGAG | Mutagenesis |
324F | 314-338 | CTCTTGTTGATATTTTAAAAGAGCG | Mutagenesis |
324R | 338-314 | CGCTCTTTTAAAATATCAACAAGAG | Mutagenesis |
2605F | 2605-2626 | CAGGTTGTTTCTGTTGGTGCTG | Amplification |
2953R | 2937-2953 | CCGCCAGCAATAGCACC | Amplification |
Anc_asF | 305-324 | GTAGAGATTCTCTTGTTGAC | Allele-specific |
mut323_asF | 305-323 | GTAGAGATTCTCTTGTTGG | Allele-specific |
mut324_asF | 304-324 | GGTAGAGATTCTCTTGTTGAT | Allele-specific |
phiXpD_asR | 625-603 | GCAATAAACTCAACAGGAGCAGG | Amplification |
phiXpB_F | 5337-5360 | CTCAAATTTATGCGCGCTTCGATA | qPCR Primer |
phiXpB_M | 5377-7 | CCAACCTGCAGAGTTT | qPCR Probe |
phiXpB_R | 27-7 | TTCTGCGTCATGGAAGCGATA | qPCR Primer |
phiXpD_F | 400-424 | TTACTGAACAATCCGTACGTTTCCA | qPCR Primer |
phiXpD_M | 427-443 | CCGCTTTGGCCTCTATT | qPCR Probe |
phiXpD_R | 466-457 | ACGGCAGAAGCCTGAATGAG | qPCR Primer |