Figure 3From: Evolution of plant phage-type RNA polymerases: the genome of the basal angiosperm Nuphar advena encodes two mitochondrial and one plastid phage-type RNA polymerasesGFP fusion constructs for targeting experiments. Amino-terminal RpoT sequences (white bars) were translationally fused to GFP S65C (green bars) in plasmid pOL (see "Methods"). The lengths of the fragments are given by nucleotide numbers (+1 is the 5' end of the 5'-UTR). The translation start is indicated by Met or Met* (CUG-coded start codon); the crossed Met (Met*) position designates the mutation introduced at that position to prevent initiation of translation.Back to article page