Figure 6

Microarray design comparing fertile and non-fertile lines. (A) Design for the microarray experiment comparing gene expression in one fertile and four non-fertile lines. The fertile line bears an introgression that may or may not include the light-gray region, but definitely excludes the portion where factor 1 is located (83B4-84B1). Black arrows indicate inversion previously known in D. simulans and D. mauritiana, in relation to D. melanogaster. (B) Microarray design showing all the comparisons among lines. In our design, four independently obtained lines with non-fertile phenotypes were compared with a fertile reference. These lines only differ in the presence of a small segment of D. mauritiana where factor 1 is present. In the fertile line this segment is present in heterozygosity with the homologous segment from D. simulans, whereas the segment is homozygous D. mauritiana/D. mauritiana in the four non-fertile lines.