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Table 2 Amplification of shared single copy nuclear genes in Brassicaceae

From: Identification of shared single copy nuclear genes in Arabidopsis, Populus, Vitis and Oryzaand their phylogenetic utility across various taxonomic levels

Marker At2 g21870 At2 g32520 At3 g47810 At4 g31720 At4 g33250 At5 g47570 At5 g63135 At5 g63135 At2 g13360 At4 g15790 At4 g37830 At5 g23290
RT-PCR             
Brassica rapa 1 1 1 1 1 1 1 1 1 0 1 1
Brassica oleracea 1 1 1 1 1 1 1 1 1 0 1 1
Moricandria arvensis 1 1 1 1 1 1 1 1 1 0 1 1
Brassica repanda 1 1 1 1 1 1 1 1 1 1 1 1
Erucastrum canariense 1 1 1 1 1 1 0 1 1 1 0 1
Schouwia thebaica 1 1 1 1 1 1 0 1 1 1 0 1
Sisymbrium irio 1 1 1 1 1 1 1 1 1 0 1 1
Arabidopsis thaliana 1 1 1 1 1 1 0 1 1 0 1 1
Olimarabidopsis pumila 1 1 1 1 1 1 0 1 1 0 1 1
Chorispora tenella 1 1 1 1 1 1 0 1 1 0 1 1
Athionema saxatile 1 1 1 1 1 1 0 1 1 1 0 1
Medicago truncatula 1 1 1 1 1 1 0 1 1 1 0 1
Band Size in bp 450 450 350 250 210 300 250 300 900 150 150 150
PCR             
Brassica rapa 3 1 1 3 3 3 2 1 1 1 1 2
Brassica oleracea 2 1 1 2 2 2 1 1 1 2 1 1
Sisymbrium irio 1 1 0 1 1 1 1 1 1 1 1 2
Arabidopsis thaliana 1 1 1 1 1 2 1 1 1 1 1 2
Olimarabidopsis pumila 1 1 0 1 2 1 2 1 1 1 1 2
Band Sizes in bp 700 1000 2000 1300 1200 2000 2000 3000 500 1000 1500 700
   800 600 600 1100 700 400 300 200 500   
   700      200      
  1. PCR and RT-PCR amplification results from Brassicaceae showing number and size of bands. The number of bands amplified is indicated (0-5).