Table 1 Primers used for 3' and 5'RACE.
From: Molecular evolution of the crustacean hyperglycemic hormone family in ecdysozoans
Primer | Species | Sequence (5' to 3') | Tm (°C) |
|---|---|---|---|
DamITP-Up1 | Daphnia magna | TCCAGCGGCCATCATTCGTTGTCC | 71 |
DamITP-Up2 | Daphnia magna | CCTCGACCGCATTTGCCAAGACTG | 71 |
DamITP-Down1 | Daphnia magna | GGACAACGAATGATGGCCGCTGGA | 71 |
DamITP-Down2 | Daphnia magna | ACTGGGAAGCACTGCGAGCAAAACC | 72 |
FocITP-Up1 | Folsomia candida | GCCAAGCTGGACAGAATTTGCGACGAT | 73 |
FocITP-Up2 | Folsomia candida | ACAACTCCACTCGCTATGCAGGTCGAA | 73 |
FocITP-Down1 | Folsomia candida | TTCGACCTGCATAGCGAGTGGAGTTGT | 73 |
FocITP-Down2 | Folsomia candida | ATCGTCGCAAATTCTGTCCAGCTTGGC | 73 |