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Table 2 Analysis of molecular variance (AMOVA) using mitochondrial control region sequences for 299 individuals of Galaxias maculatus collected over the study area (all samples) and a subset of locations from northern Chilean Patagonia (90 individuals).

From: Surviving historical Patagonian landscapes and climate: molecular insights from Galaxias maculatus

 

% of variation (df)

Grouping

Among groups

Among populations within groups

Within populations

All samples

   

   1A. Lake/River

61.00 (35)

-

39.00 (263)

   1B. River drainage system

29.94 (18)

32.08 (16)

37.98 (254)

   1C. Current ocean drainage

3.68 (1)

58.76 (33)

37.55 (254)

   1D. Ancient ocean (pre-LGM) drainage

15.55 (1)

49.41 (33)

35.04 (254)

Chile

   

   2A. River basin headwaters

6.64 (1)

58.06 (15)

35.3 (110)

   2B. Collection location

13.93 (1)

51.73 (15)

34.34 (110)

  1. All samples were grouped according to (A) lake/river from which individuals were collected, (B) the freshwater basin containing each lake/river, and ocean (Pacific or Atlantic) into which each basin, (C) currently drains, or (D) histocially drained [i.e. ancient refers to the conditions before the last glacial maximum (LGM) approximately 10,000-20,000 BP]. Further post-hoc regional analysis was conducted for a subset of collection locations in Chile between latitudes 34°S and 40°S. We grouped each population as "coastal" or "Andean" for AMOVA analysis based on two criteria: (2A) location of headwaters and (2B) location of collection site (Table 1). Variation is reported as a percentage of the total with degrees of freedom (d.f.).