The three strains that persisted at low relatedness did not cheat the other five strains but cheated each other. Cheating assays of the persisting strains against the population of the five out-competed strains. The points represent the mean of three assays with 95% CI. All persisting strains tended decrease in frequency, though not significantly (A). Cheating assays among the three persisting strains. Each point represents the mean of six assays with 95% CI and is expressed as the change in frequency of the strain listed in bold (B).