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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Quasispecies-like behavior observed in catalytic RNA populations evolving in a test tube

Figure 2

The quasispecies formed during the evolution of lineage 6H. Networks among genotypes were constructed using Network v. 4.5.1.0. Different clouds can be observed in each burst evaluated: (A) burst 5; (B) burst 35; and (C) burst 50. Additionally, a consolidated alignment of all sequences in the lineage shows a different network outcome (d). Spheres represent mutants present at different frequencies, denoted by the length of the radius. The color of the sphere is different than yellow if the mutant persists through bursts or recur in a different lineage. The colors are maintained in all the networks drawn. The distance between spheres is representative of the Hamming distance between the sequences. The red spheres are calculated median vectors that represent non-sampled or predicted ancestral individuals [64]. The name of the master sequences in each burst is given as follows: (A) wildtype = B16-19 ligase ribozyme; (B) MS1; and (C) MS2, according to their order of appearance.

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