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Table 4 Sequence of primers used to amplify the gene fragments

From: Estimation of divergence time between two sibling species of the Anopheles (Kerteszia) cruziicomplex using a multilocus approach

Locus Primers name Sequence of primers
Clock 5'CLKdeg3 5'-SNGGNTAYGAYTAYTAYCA-3'
  3'CLKdeg10 5'-TCNGTYTGNARCCADATCCA-3'
  5'cruziiclock 5'-TTGACGATCTGGAAAAGGTG-3'
  3'cruziiclock 5'-CTTGGTCAGGAAGCGATAGT-3'
cycle 5'CYCdeg1 5'-ARMGNMGNMGNGAYAARATGAA-3'
  3'CYCdeg1 5'-ACYTTNCCDATRTCYTTNGGRTG-3'
  5'cruziicycle 5'-CACCTACATCACCGAACTG-3'
  3'cruziicycle 5'-GACTCGGAAACGTACAGGATA-3'
Rp49 5'aquaRP1 5'-GTGAAGAAGCGGACGAAGAAGTT-3'
  3'aeaquaRP1b 5'-TCATCAGCACCTCCAGCTC-3'
RpS2 5'cruziiRP_S2 5'-GGCTACTGGGGTAACAAGA-3'
  3'cruziiRP_S2 5'-CAGRACGGAACCGCACTT-3'
RpS29 5'cruziiRP_S29b 5'-TCGCATCCSCGTAAATA-3'
  3'cruziiRP_S29 5'-TTCCKGAAGCCAATATCCT-3'
  1. Degenerate and specific primers used to amplify the different gene fragments in the two An. cruzii sibling species.
  2. The sequences of primer pairs 5'CYCdeg1 + 3'CYCdeg1 and 5'aquaRP1 + 3'aeaquaRP1b are from [39] and [40], respectively. Degenerated primers were used in preliminary amplifications to isolate initial fragments of the cycle and Clock genes. Sequence of these fragments allowed the design of the specific primers used in the population genetics analysis.