Age and temperature specific offspring production in natural isolates CB4856 and CB4857. A) The cold-seeking isolate CB4856 produces more offspring than the hot-seeking isolate CB4857 at 15°C (F
= 13.42, p = 0.0019). B) In contrast, at 23°C, LRS does not differ between CB4856 and CB4857 (F
= 14.52, p = 0.8779), but CB4857 produces significantly more offspring than CB4856 at ages 2 and 3 days which positively impacts r. Differences between strains at each age were analyzed using ANOVA with strain as the main effect. *p < 0.05, **p < 0.001, ***p < 0.0001.