|  | Estimated divergence age (Ma) ± SD [Min - Max values of 95% confidence interval] | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
Node | Divergence events | Calibration A | Calibration B | Calibration C | Calibration D | Calibration E | Calibration F | Average (MD) of calibration A-F | Van Bocxlaer et al.[2]*1 | Van der Meijden et al.[3] |
 |  | (from the Aln-2d + Tree1a combination) | (from the Aln-3d + Tree1b combination) |  |  |  | ||||
I | Split of microhylid lineage from other ranoids | 143.2 ± 12.5 [121.6-170.3] | 134.6 ± 15.6 [106.4-167.3] | 130.0 ± 14.2 [103.8-159.3] | 136.6 ± 10.3 [118.1-158.6] | 133.0 ± 10.0 [115.3-154.4] | 118.5 ± 11.5 [98.0-142.8] | 132.6 (9.6) | 127.3 ± 9.7 [109.9 - 147.9] | 116 ± 17 [87 - 153] |
II | Initial divergence of living microhylid subfamilies (Split of Phrynomerinae) | 90.3 ± 8.5 [77.6-110.6] | 82.3 ± 11.8 [61.6-107.6] | 78.9 ± 10.6 [59.9-101.4] | 92.3 ± 7.1 [80.3-108.0] | 89.3 ± 6.8 [78.0-104.5] | 75.4 ± 9.3 [58.7-95.1] | 84.8 (8.4) | 88.0 ± 6.5 [77.0 - 102.2] | 66 ± 11*3 [47 - 90] |
III | 2 nd basal split of microhylid subfamilies (Split of Hoplophryninae) | 88.7 ± 8.4 [76.3-108.6] | 80.7 ± 11.6 [60.3-105.7] | 77.4 ± 10.5 [58.7-99.6] | 87.7 ± 6.4 [77.1-102.1] | 84.6 ± 6.1 [74.7-98.6] | 70.9 ± 8.8 [55.1-89.6] | 81.7 (8.2) | 83.9 ± 5.9 [74.2 - 97.1] | NS (57 - 66)*4 |
IV | Split of Gastrophryninae + (Cophylinae + Scaphiophryninae) clade from other microhylids | 85.8 ± 8.0 [74.2-105.1] | 78.0 ± 11.3 [58.2-102.2] | 74.8 ± 10.2 [56.6-96.4] | 83.8 ± 5.8 [74.3-97.1] | 80.8 ± 5.6 [72.0-93.8] | 67.3 ± 8.5 [52.2-85.3] | 78.4 (8.1) | NA | NA |
V | Split of Kalophryninae from Asterophryinae + (Dyscophinae + Microhylinae) clade | 81.4 ± 7.7 [70.7-100.1] | 73.9 ± 10.8 [54.8-97.1] | 70.9 ± 9.8 [53.4-91.6] | 81.8 ± 5.6 [72.7-94.5] | 78.8 ± 5.3 [70.5-91.3] | 65.3 ± 8.3 [50.5-83.0] | 75.3 (7.9) | 76.8 ± 5.0*2 [68.9 - 88.7] | NA |
VI | Split of Asterophryinae from Dyscophinae + Microhylinae clade | 76.6 ± 7.1 [67.2-94.2] | 69.3 ± 10.3 [51.2-91.3] | 66.5 ± 9.3 [49.9-86.2] | 76.8 ± 5.0 [68.9-88.6] | 73.9 ± 4.9 [66.9-85.7] | 60.8 ± 7.9 [46.8-77.7] | 70.6 (7.7) | 73.6 ± 4.5 [66.7 - 84.5] | 57 ± 10 [40 - 79] |
VII | Split of Dyscophinae and Microhylinae | 73.6 ± 6.8 [65.3-90.7] | 66.5 ± 10.0 [49.0-87.9] | 63.7 ± 9.0 [47.7-83.0] | 72.6 ± 4.4 [66.4-83.3] | 69.7 ± 4.2 [65.1-80.6] | 56.8 ± 7.5 [43.5-72.9] | 67.2 (7.6) | 68.7 ± 3.4 [65.1 - 77.6] | 55 ± 10 [39 - 76] |
VIII | Split of Gastrophryninae from Cophylinae + Scaphiophryninae clade | 83.9 ± 8.0 [72.1-103.0] | 76.3 ± 11.1 [56.7-100.2] | 73.1 ± 10.0 [55.1-94.3] | 80.4 ± 6.0 [70.1-93.8] | 77.6 ± 5.8 [67.8-90.7] | 64.7 ± 8.3 [49.8-82.3] | 76.0 (7.9) | NA | NA |
IX | Split of Cophylinae and Scaphiophryninae | 72.1 ± 7.8 [59.8-90.0] | 65.4 ± 10.1 [47.8-87.3] | 62.7 ± 9.2 [46.4-82.3] | 71.2 ± 6.8 [59.0-85.4] | 68.9 ± 6.6 [57.1-83.0] | 57.5 ± 8.1 [43.1-74.8] | 66.3 (6.8) | NA | 53 ± 9 [38 - 74] |
X | Split of Gastrophrynoides from other asterophryines | 53.7 ± 6.5 [43.1-68.5] | 48.5 ± 8.2 [34.3-66.2] | 46.5 ± 7.5 [33.4-62.6] | 50.1 ± 5.5 [40.3-61.7] | 48.4 ± 5.3 [39.0-59.8] | 39.7 ± 6.3 [28.7-53.3] | 47.8 (5.4) | NA | NA |
XI | Initial divergence of non- Gastrophrynoides asterophryines | 22.7 ± 4.3 [15.5-32.3] | 20.5 ± 4.6 [12.9-30.7] | 19.7 ± 4.2 [12.6-29.1] | 30.4 ± 4.3 [22.8-39.6] | 29.4 ± 4.2 [22.0-38.6] | 24.1 ± 4.5 [16.5-33.8] | 24.5 (5.4) | 26.8 ± 4.0 [19.8 - 35.3] | 20 ± 5 [12 - 30] |