Figure 1

Genetic lineages within the Pyura stolonifera species complex. A) regions in which members of the species complex were collected for this study (see Table 1 for details); B) minimum evolution tree based on combined sequence data from 4 loci; support for nodes is indicated as bootstrap values (≥ 50%) from minimum evolution and parsimony analyses, and as posterior probabilities (≥ 95%) from Bayesian inference; C) haplotype network constructed from ANT sequences of P. herdmani and D) linearised neighbor-joining phylogeny based on sequences of the COI gene of P. herdmani; bootstrap values are indicated, and P. stolonifera was used as outgroup (not shown); E) haplotype network of ANT sequences of Pyura sp. and F) haplotype network of COI sequences of P. dalbyi. (Acronyms: ANT = nuclear Adenine Nucleotide Transporter gene; COI = mitochondrial cytochrome oxidase subunit I gene; SE = southeastern).